Alhagi sparsifolia Shap. （Fabaceae） is a spiny, perennial herb. The species grows in the salinized, arid regions in North China. This study investigated the response characteristics of the root growth and the dis- t...Alhagi sparsifolia Shap. （Fabaceae） is a spiny, perennial herb. The species grows in the salinized, arid regions in North China. This study investigated the response characteristics of the root growth and the dis- tribution of one-year-old A. sparsifolia seedlings to different groundwater depths in controlled plots. The eco- logical adaptability of the root systems of A. sparsifolia seedlings was examined using the artificial digging method. Results showed that： （1） A. sparsifolia seedlings adapted to an increase in groundwater depth mainly through increasing the penetration depth and growth rate of vertical roots. The vertical roots grew rapidly when soil moisture content reached 3%-9%, but slowly when soil moisture content was 13%-20%. The vertical roots stopped growing when soil moisture content reached 30% （the critical soil moisture point）. （2） The morphological plasticity of roots is an important strategy used by A. sparsifolia seedlings to obtain water and adapt to dry soil conditions. When the groundwater table was shallow, horizontal roots quickly expanded and tillering increased in order to compete for light resources, whereas when the groundwater table was deeper, vertical roots developed quickly to exploit space in the deeper soil layers. （3） The decrease in groundwater depth was probably respon- sible for the root distribution in the shallow soil layers. Root biomass and surface area both decreased with soil depth. One strategy of A. sparsifolia seedlings in dealing with the increase in groundwater depth is to increase root biomass in the deep soil layers. The relationship between the root growth/distribution of A. sparsifolia and the depth of groundwater table can be used as guidance for harvesting A. sparsifolia biomass and managing water resources for forage grasses. It is also of ecological significance as it reveals how desert plants adapt to arid environments.展开更多
Water relations of Alhagi sparsifolia Shap. at the transition zone between oases and sandy desert were studied in the southern fringe of Taklamakan Desert. Results showed that A. sparsifolia maintained the positive tu...Water relations of Alhagi sparsifolia Shap. at the transition zone between oases and sandy desert were studied in the southern fringe of Taklamakan Desert. Results showed that A. sparsifolia maintained the positive turgor during the summer. The steady high predawn water potential (psi(P)) indicated A. sparsifolia had sufficient hydration and water supply in growing season. In July, water deficit caused by drought stress had no effect on the transpiration of A. sparsifolia. Therefore, drought stress is not a main factor affecting the survival of plants. The physiological adaptation to drought of A. sparsifolia was shown mainly at the leaf level by significant difference (DeltaII) and relative water content (RWC) between the osmotic pressure at full turgor and at turgor loss, by occurring of osmotic adjustment, by high percentage of dry mass-related water content ( WCsat), by RWC at turgor loss point ( RWCp) in stable level and low RWC of the saturated symplast (RWCsym). However, the morphological features of transpiring surface reduction and deep root system seem to be the main way for the plant to adapt to the extreme drought environment. Result also suggests that one time of irregular irrigation in summer will not be helpful to recover water status of A. sparsifolia in location where the water table is very low.展开更多
The effect of variation in water supply on woody seedling growth in arid environments remain poorly known.The subshrub Alhagi sparsifolia Shap.（Leguminosae）,distributed in the southern fringe of the Taklimakan Deser...The effect of variation in water supply on woody seedling growth in arid environments remain poorly known.The subshrub Alhagi sparsifolia Shap.（Leguminosae）,distributed in the southern fringe of the Taklimakan Desert,Xinjiang,northwestern China,has evolved deep roots and is exclusively dependent on groundwater,and performs a crucial role for the local ecological safety.In the Cele oasis,we studied the responses of A.sparsifolia seedling roots to water supplement at 10 and 14 weeks under three irrigation treatments （none water supply of 0 m3/m2 （NW）,middle water supply of 0.1 m3/m2 （MW）,and high water supply of 0.2 m3/m2 （HW））.The results showed that the variations of soil water content （SWC） significantly influenced the root growth of A.sparsifolia seedlings.The leaf area,basal diameter and crown diameter were significantly higher in the HW treatment than in the other treatments.The biomass,root surface area （RSA）,root depth and relative growth rate （RGR） of A.sparsifolia roots were all significantly higher in the NW treatment than in the HW and MW treatments at 10 weeks.However,these root parameters were significantly lower in the NW treatment than in the other treatments at 14 weeks.When SWC continued to decline as the experiment went on （until less than 8％ gravimetric SWC）,the seedlings still showed drought tolerance through morphological and physiological responses,but root growth suffered serious water stress compared to better water supply treatments.According to our study,keeping a minimum gravimetric SWC of 8％ might be important for the growth and establishment of A.sparsifolia during the early growth stage.These results will not only enrich our knowledge of the responses of woody seedlings to various water availabilities,but also provide a new insight to successfully establish and manage A.sparsifolia in arid environments,further supporting the sustainable development of oases.展开更多
[ Objective] The aim was to establish effective method for endogenous hormone extraction and explore conditions of chromatographic analysis for three endogenous hormones in rhizome of Alhagi sparsifolia. [ Methed ] Ac...[ Objective] The aim was to establish effective method for endogenous hormone extraction and explore conditions of chromatographic analysis for three endogenous hormones in rhizome of Alhagi sparsifolia. [ Methed ] Activol （GA3 ）, zeatin （ZR） and indole-3-acetic acid （IAA） in rhizome were separated and measured as per RP-HPLC. [Result] The average recovery rates of GA3, ZR and IAA were 98.3%, 90.3% and 101.3%, respectively, indicating that the method is suitable for quantitative analysis with little errors. The chromatographic conditions were as follows： methanol/0. 75% of acetic acid at 45：55 （mobile phase） ; flow speed at 0.7 ml/min; wavelength at 254 nm; column temperature at 25 ℃. [Conclusion] The research preliminarily established HPLC conditions for separation of endogenous hormones in rhizome of Alhagi sparsifolia.展开更多
目的:提取分离骆驼刺茎枝粗多糖,测定其理化性质及抗氧化活性。方法:采用水提醇沉法提取骆驼刺茎枝粗多糖,硫酸苯酚法测定总糖含量,考马斯亮蓝法测定蛋白含量,咔唑硫酸法测定糖醛酸含量,GC法测定单糖组成及相对摩尔比;通过测定骆驼刺茎...目的:提取分离骆驼刺茎枝粗多糖,测定其理化性质及抗氧化活性。方法:采用水提醇沉法提取骆驼刺茎枝粗多糖,硫酸苯酚法测定总糖含量,考马斯亮蓝法测定蛋白含量,咔唑硫酸法测定糖醛酸含量,GC法测定单糖组成及相对摩尔比;通过测定骆驼刺茎枝多糖的还原力,及其对1,1-二苯基-2-三硝基苯肼(DPPH)自由基和羟自由基的清除力,评价骆驼刺茎枝多糖的体外抗氧化活性。结果:水提醇沉制备的骆驼刺茎枝粗多糖总糖含量为73.2%,其中糖醛酸占粗多糖总糖含量的27.2%;蛋白质含量为17.6%;骆驼刺茎枝多糖由Rha、Ara、Xyl、Man、Glc、Gal、Glc A和Gal A 8种单糖组成,相对摩尔比为1.05∶1.00∶1.25∶0.52∶3.05∶1.31∶0.47∶4.78;骆驼刺茎枝多糖的还原力、对DPPH与羟基自由基的清除率随多糖浓度的增大而增强。结论:初步提取分离了骆驼刺茎枝粗多糖,研究了其理化性质和体外抗氧化活性,为骆驼刺的开发利用提供研究基础。展开更多
基金supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-EW-316)the National Natural Science Foundation of China (31070477,30870471)the West Light Foundation of the Chinese Academy of Sciences (XBBS201105)
文摘Alhagi sparsifolia Shap. （Fabaceae） is a spiny, perennial herb. The species grows in the salinized, arid regions in North China. This study investigated the response characteristics of the root growth and the dis- tribution of one-year-old A. sparsifolia seedlings to different groundwater depths in controlled plots. The eco- logical adaptability of the root systems of A. sparsifolia seedlings was examined using the artificial digging method. Results showed that： （1） A. sparsifolia seedlings adapted to an increase in groundwater depth mainly through increasing the penetration depth and growth rate of vertical roots. The vertical roots grew rapidly when soil moisture content reached 3%-9%, but slowly when soil moisture content was 13%-20%. The vertical roots stopped growing when soil moisture content reached 30% （the critical soil moisture point）. （2） The morphological plasticity of roots is an important strategy used by A. sparsifolia seedlings to obtain water and adapt to dry soil conditions. When the groundwater table was shallow, horizontal roots quickly expanded and tillering increased in order to compete for light resources, whereas when the groundwater table was deeper, vertical roots developed quickly to exploit space in the deeper soil layers. （3） The decrease in groundwater depth was probably respon- sible for the root distribution in the shallow soil layers. Root biomass and surface area both decreased with soil depth. One strategy of A. sparsifolia seedlings in dealing with the increase in groundwater depth is to increase root biomass in the deep soil layers. The relationship between the root growth/distribution of A. sparsifolia and the depth of groundwater table can be used as guidance for harvesting A. sparsifolia biomass and managing water resources for forage grasses. It is also of ecological significance as it reveals how desert plants adapt to arid environments.
文摘Water relations of Alhagi sparsifolia Shap. at the transition zone between oases and sandy desert were studied in the southern fringe of Taklamakan Desert. Results showed that A. sparsifolia maintained the positive turgor during the summer. The steady high predawn water potential (psi(P)) indicated A. sparsifolia had sufficient hydration and water supply in growing season. In July, water deficit caused by drought stress had no effect on the transpiration of A. sparsifolia. Therefore, drought stress is not a main factor affecting the survival of plants. The physiological adaptation to drought of A. sparsifolia was shown mainly at the leaf level by significant difference (DeltaII) and relative water content (RWC) between the osmotic pressure at full turgor and at turgor loss, by occurring of osmotic adjustment, by high percentage of dry mass-related water content ( WCsat), by RWC at turgor loss point ( RWCp) in stable level and low RWC of the saturated symplast (RWCsym). However, the morphological features of transpiring surface reduction and deep root system seem to be the main way for the plant to adapt to the extreme drought environment. Result also suggests that one time of irregular irrigation in summer will not be helpful to recover water status of A. sparsifolia in location where the water table is very low.
基金funded by the Knowledge Innovation Program of the Chinese Academy of Sciences(KZCX2-EW-316)the National Natural Science Foundation of China(41001171,31070477,30870471)+2 种基金the Western Light Foundation of the Chinese Academy of Sciences(XBBS201105)the Key Program of Joint Funds of the National Natural Science Foundation of Chinathe Government of Xinjiang Uygur Autonomous Region of China(U1203201)
文摘The effect of variation in water supply on woody seedling growth in arid environments remain poorly known.The subshrub Alhagi sparsifolia Shap.（Leguminosae）,distributed in the southern fringe of the Taklimakan Desert,Xinjiang,northwestern China,has evolved deep roots and is exclusively dependent on groundwater,and performs a crucial role for the local ecological safety.In the Cele oasis,we studied the responses of A.sparsifolia seedling roots to water supplement at 10 and 14 weeks under three irrigation treatments （none water supply of 0 m3/m2 （NW）,middle water supply of 0.1 m3/m2 （MW）,and high water supply of 0.2 m3/m2 （HW））.The results showed that the variations of soil water content （SWC） significantly influenced the root growth of A.sparsifolia seedlings.The leaf area,basal diameter and crown diameter were significantly higher in the HW treatment than in the other treatments.The biomass,root surface area （RSA）,root depth and relative growth rate （RGR） of A.sparsifolia roots were all significantly higher in the NW treatment than in the HW and MW treatments at 10 weeks.However,these root parameters were significantly lower in the NW treatment than in the other treatments at 14 weeks.When SWC continued to decline as the experiment went on （until less than 8％ gravimetric SWC）,the seedlings still showed drought tolerance through morphological and physiological responses,but root growth suffered serious water stress compared to better water supply treatments.According to our study,keeping a minimum gravimetric SWC of 8％ might be important for the growth and establishment of A.sparsifolia during the early growth stage.These results will not only enrich our knowledge of the responses of woody seedlings to various water availabilities,but also provide a new insight to successfully establish and manage A.sparsifolia in arid environments,further supporting the sustainable development of oases.
基金Initial Funding of Doctor in Xinjiang University(7020428027)National Innovation Experiment Program for University Students of China (101075512)
文摘[ Objective] The aim was to establish effective method for endogenous hormone extraction and explore conditions of chromatographic analysis for three endogenous hormones in rhizome of Alhagi sparsifolia. [ Methed ] Activol （GA3 ）, zeatin （ZR） and indole-3-acetic acid （IAA） in rhizome were separated and measured as per RP-HPLC. [Result] The average recovery rates of GA3, ZR and IAA were 98.3%, 90.3% and 101.3%, respectively, indicating that the method is suitable for quantitative analysis with little errors. The chromatographic conditions were as follows： methanol/0. 75% of acetic acid at 45：55 （mobile phase） ; flow speed at 0.7 ml/min; wavelength at 254 nm; column temperature at 25 ℃. [Conclusion] The research preliminarily established HPLC conditions for separation of endogenous hormones in rhizome of Alhagi sparsifolia.
文摘目的:提取分离骆驼刺茎枝粗多糖,测定其理化性质及抗氧化活性。方法:采用水提醇沉法提取骆驼刺茎枝粗多糖,硫酸苯酚法测定总糖含量,考马斯亮蓝法测定蛋白含量,咔唑硫酸法测定糖醛酸含量,GC法测定单糖组成及相对摩尔比;通过测定骆驼刺茎枝多糖的还原力,及其对1,1-二苯基-2-三硝基苯肼(DPPH)自由基和羟自由基的清除力,评价骆驼刺茎枝多糖的体外抗氧化活性。结果:水提醇沉制备的骆驼刺茎枝粗多糖总糖含量为73.2%,其中糖醛酸占粗多糖总糖含量的27.2%;蛋白质含量为17.6%;骆驼刺茎枝多糖由Rha、Ara、Xyl、Man、Glc、Gal、Glc A和Gal A 8种单糖组成,相对摩尔比为1.05∶1.00∶1.25∶0.52∶3.05∶1.31∶0.47∶4.78;骆驼刺茎枝多糖的还原力、对DPPH与羟基自由基的清除率随多糖浓度的增大而增强。结论:初步提取分离了骆驼刺茎枝粗多糖,研究了其理化性质和体外抗氧化活性,为骆驼刺的开发利用提供研究基础。
