Adventitious shoots were successfully regenerated from hypocotyl explants of in vitro cultures of Euonymus japonicus Cu zhi. Hypocotyl slices were cultured on Murashige and Skoog （MS） and B5 basal medium supplemente...Adventitious shoots were successfully regenerated from hypocotyl explants of in vitro cultures of Euonymus japonicus Cu zhi. Hypocotyl slices were cultured on Murashige and Skoog （MS） and B5 basal medium supplemented with varied concentration of different plant growth-regulators, e.g., α-naphthaleneacetic acid （NAA） and indole-3-butyric acid （IBA） in combination with 6-benzylaminopurine （6-BA） and kinetin. The study showed that shoots could be directly regenerated from hypocotyl explants without the intervening callus phase; MS medium was more suitable for adventitious shoots regeneration. The ability of hypocotyls segments to produce shoots varied depending upon their position on the seedlings. The highest regeneration rate was obtained with hypocotyl segments near to the cotyledon cultured on MS basal medium supplemented with 1.5 mg L^-1 6-BA and 0.05 mg L^-1 NAA （63.64%）. The regenerated shoots were readily elongated on the same medium as used for multiplication and rooted on half-strength MS basal medium supplemented with 1.0 mg L^-1 IBA and 100 mg L^-1 activated carbon. After being transferred to greenhouse conditions, 96% of the plantlets were successfully acclimatized. This regeneration system is applied for genetic transformation now.展开更多
以加工番茄BO 2和BO 4无菌苗的子叶和下胚轴为外植体,以M S为基本培养基,研究了不同浓度的ZT,6-BA与不同浓度的IAA组合对外植体芽诱导的影响。结果表明,在不同浓度的ZT和IAA组合中,对2个番茄品种的子叶和下胚轴外植体芽诱导均以M S+1.0 ...以加工番茄BO 2和BO 4无菌苗的子叶和下胚轴为外植体,以M S为基本培养基,研究了不同浓度的ZT,6-BA与不同浓度的IAA组合对外植体芽诱导的影响。结果表明,在不同浓度的ZT和IAA组合中,对2个番茄品种的子叶和下胚轴外植体芽诱导均以M S+1.0 m g/L ZT+0.05 m g/L IAA培养基为最好,BO 2品种子叶和下胚轴的出芽率分别为32.7%和24.5%,BO 4品种的分别为37.3%和28.2%;在不同浓度的6-BA和IAA组合中,诱导BO 2外植体出芽率最高的培养基是M S+2.0 m g/L 6-BA+0.2 m g/L IAA,子叶和下胚轴的出芽率分别为27.3%和14.5%;诱导BO 4品种子叶和下胚轴出芽率最高的培养基分别为M S+1.0 m g/L 6-BA+0.2 m g/LIAA和M S+2.0 m g/L 6-BA+0.2 m g/L IAA,子叶和下胚轴的出芽率均为28.2%;在M S+0.1 m g/L IAA培养基上,加工番茄BO 2再生芽1周左右就能长出根,形成完整的小植株。展开更多
文摘Adventitious shoots were successfully regenerated from hypocotyl explants of in vitro cultures of Euonymus japonicus Cu zhi. Hypocotyl slices were cultured on Murashige and Skoog （MS） and B5 basal medium supplemented with varied concentration of different plant growth-regulators, e.g., α-naphthaleneacetic acid （NAA） and indole-3-butyric acid （IBA） in combination with 6-benzylaminopurine （6-BA） and kinetin. The study showed that shoots could be directly regenerated from hypocotyl explants without the intervening callus phase; MS medium was more suitable for adventitious shoots regeneration. The ability of hypocotyls segments to produce shoots varied depending upon their position on the seedlings. The highest regeneration rate was obtained with hypocotyl segments near to the cotyledon cultured on MS basal medium supplemented with 1.5 mg L^-1 6-BA and 0.05 mg L^-1 NAA （63.64%）. The regenerated shoots were readily elongated on the same medium as used for multiplication and rooted on half-strength MS basal medium supplemented with 1.0 mg L^-1 IBA and 100 mg L^-1 activated carbon. After being transferred to greenhouse conditions, 96% of the plantlets were successfully acclimatized. This regeneration system is applied for genetic transformation now.
文摘以加工番茄BO 2和BO 4无菌苗的子叶和下胚轴为外植体,以M S为基本培养基,研究了不同浓度的ZT,6-BA与不同浓度的IAA组合对外植体芽诱导的影响。结果表明,在不同浓度的ZT和IAA组合中,对2个番茄品种的子叶和下胚轴外植体芽诱导均以M S+1.0 m g/L ZT+0.05 m g/L IAA培养基为最好,BO 2品种子叶和下胚轴的出芽率分别为32.7%和24.5%,BO 4品种的分别为37.3%和28.2%;在不同浓度的6-BA和IAA组合中,诱导BO 2外植体出芽率最高的培养基是M S+2.0 m g/L 6-BA+0.2 m g/L IAA,子叶和下胚轴的出芽率分别为27.3%和14.5%;诱导BO 4品种子叶和下胚轴出芽率最高的培养基分别为M S+1.0 m g/L 6-BA+0.2 m g/LIAA和M S+2.0 m g/L 6-BA+0.2 m g/L IAA,子叶和下胚轴的出芽率均为28.2%;在M S+0.1 m g/L IAA培养基上,加工番茄BO 2再生芽1周左右就能长出根,形成完整的小植株。
