A method of reduced CO\|difference spectrum was established to study the cytochrome P450 activity of the whole cell of \%Candida tropicalis\% during the alkane converting process. Using this method, the cytochrome P45...A method of reduced CO\|difference spectrum was established to study the cytochrome P450 activity of the whole cell of \%Candida tropicalis\% during the alkane converting process. Using this method, the cytochrome P450 activities of the whole cells that were cultured in the different concentrations of alkane were studied. The results showed that the 5% alkane could induce the cytochrome P450 activity obviously but not inhibit the growth of cells, so it was determined preliminarily that the alkane concentration of the seed medium was 5%. The cytochrome P450 activities of dicarboxylic acid (DCA) fermentation processing were further studied. During the exponential phase of growth, the cytochrome P450 activity increased smoothly. However, during the phase of production of dicarboxylic acid, the cytochrome P450 activities increased rapidly after a sort decrease. The results still showed that the rate of production of dicarboxylic acid increased with the cytochrome P450 activity.展开更多
The effects of fermentative conditions such as control mode of pH,concentration of cells and dissolved oxygen on fermentation of 1,12-dodecanedioic acid from n-dodecane by a mutant,Candida tropicalis SP-UV-56,were stu...The effects of fermentative conditions such as control mode of pH,concentration of cells and dissolved oxygen on fermentation of 1,12-dodecanedioic acid from n-dodecane by a mutant,Candida tropicalis SP-UV-56,were studied.Suitable concentration of cells is 15g/L～17g/L,dissolved oxygen level is preferably kept at 20%～30%.The control mode of pH by gradually going up adjusted with NaOH solution was suggested while pH range is 7.4～8.4.Under the optimal conditions the DCA12 yield of 166g/L was obtained after 144h cultivation.This shows that the mutant is a high-yield strain for 1,12-dodecanedioic acid production.展开更多
A mutant of Candida tropicalis FYD-2 was obtained from its parental strain SFP-1186 by ultraviolet treatments.On shaking flask,the yield of mixed dicarboxylic acid(DCA) by the mutant was 21.4% higher than that by its ...A mutant of Candida tropicalis FYD-2 was obtained from its parental strain SFP-1186 by ultraviolet treatments.On shaking flask,the yield of mixed dicarboxylic acid(DCA) by the mutant was 21.4% higher than that by its ancestor.The amount of mixed DCA reached 156g/L for 120h incubation in a 10 L autoconrolled fermentor where the culture medium contained 25% n-paraffin.The process of induced and screening mutant was introduced and the time course of fermentation in 10 L fermentor was discussed.展开更多
文摘A method of reduced CO\|difference spectrum was established to study the cytochrome P450 activity of the whole cell of \%Candida tropicalis\% during the alkane converting process. Using this method, the cytochrome P450 activities of the whole cells that were cultured in the different concentrations of alkane were studied. The results showed that the 5% alkane could induce the cytochrome P450 activity obviously but not inhibit the growth of cells, so it was determined preliminarily that the alkane concentration of the seed medium was 5%. The cytochrome P450 activities of dicarboxylic acid (DCA) fermentation processing were further studied. During the exponential phase of growth, the cytochrome P450 activity increased smoothly. However, during the phase of production of dicarboxylic acid, the cytochrome P450 activities increased rapidly after a sort decrease. The results still showed that the rate of production of dicarboxylic acid increased with the cytochrome P450 activity.
文摘The effects of fermentative conditions such as control mode of pH,concentration of cells and dissolved oxygen on fermentation of 1,12-dodecanedioic acid from n-dodecane by a mutant,Candida tropicalis SP-UV-56,were studied.Suitable concentration of cells is 15g/L～17g/L,dissolved oxygen level is preferably kept at 20%～30%.The control mode of pH by gradually going up adjusted with NaOH solution was suggested while pH range is 7.4～8.4.Under the optimal conditions the DCA12 yield of 166g/L was obtained after 144h cultivation.This shows that the mutant is a high-yield strain for 1,12-dodecanedioic acid production.
文摘A mutant of Candida tropicalis FYD-2 was obtained from its parental strain SFP-1186 by ultraviolet treatments.On shaking flask,the yield of mixed dicarboxylic acid(DCA) by the mutant was 21.4% higher than that by its ancestor.The amount of mixed DCA reached 156g/L for 120h incubation in a 10 L autoconrolled fermentor where the culture medium contained 25% n-paraffin.The process of induced and screening mutant was introduced and the time course of fermentation in 10 L fermentor was discussed.
基金sponsored by the Industrialization of Scientific Research Promotion Projects of Collegesand Universities in Jiangsu Province ( No. JH10-12) the Qing-Lan Project of Educational Department ofJiangsu Province ( 2008) the Natural Science Foundation of Jiangsu Province ( No. SBK200921231)
