All-transRetinoic Acid Regulates Th1/Th2 Balance in CD4+T cells When GATA-3 is Deficient

The essential effect of vitamin A on immune function occurs through various mechanisms including direct effect on ThloTh2 balance modulation. However, it is unclear whether or not vitamin A can regulate Thl-Th2 balance under a strong Thl-polarizing condition. Therefore, the purpose of our study was to examine the effect of vitamin A metabolite allotrans retinoic acid （ATRA） on ThloTh2 differentiation in CD4~ T cells under GATA-3 deficiency, which can induce Thl-polarizing condition. In the present study, GATA-3 deficiency T cells were induced by siRNA and checked by real-time quantitative PCR and western blot. GATA-3 deficiency CD4＋ T cells and normal CD4＋ T were treated for 48 h with or without ATRA.

Mechanism of all-transretinoic acid increasing retinoblastoma sensitivity to vincristine

Objective:To explore the mechanism of all-transretinoic acid(ATRA) increasing retinoblastoma(RB) sensitivity to vincristine,and the inhibiting effect of vincristine combined with ATRA treatment on the SO-RB50 cell proliferation.Methods:SO-RB50 cells were cultivated by routine culture method.Different concentrations of vincristine or ATRA were added into culture solution.After 48 h.cell counting kit-8 was used to detect the median inhibitory concentration(IC_(50)) of vincristine combined with ATRT treatment to SO-RB50 cells.SO-RB50 cells were divided into drug combination group,vincristine group,ATRA group and control group.Different drugs were added into the culture solution respectively for cell culture based on the IC_(50) value.Cell counting kit-8 was used to detect the cell proliferation every 24-h cultivation.After continuous determination for 6 d,data was processed to draw the cell growth curve.After drug use for 72 h,flow cytometry was used to detect the proportion of different cell cycles of SO-RB50 cells in each group.After drug use for 48 h,annexin V/propidium iodide method was used to detect the SO-RB50 cell apoptosis in each group.Results:The IC_(50) value of vincristine treatment on the SO-RB50 cells was 0.11 μmol/L,and ATRT was 12.84 μmol/L.The cell growth curve in control group rose gradually along with the extended culture time,but after vincristine and ATRA treatment,the cell growth curve was smooth and steady.The cell increment was the least in drug combination group and its cell growth curve was the smoothest.There was significant difference in A_(450) 48 h and 72 h after treatment(F_(grouping)=77.316,P<0.001;F_(time)=86.985,P<0.001).Compared with control group.A_(450) value in drug combination group,vincristine group,ATRA group was significantly lower(P<0.001).Compared with control group,the G_2/M phase cell proportion in vincristine group was significantly increased,while the G_0/G_1 phase cell proportion was significantly decreased:the G_0/G_1 phase cell proportion in ATRA group was significantly increased,while the S phase cell proportion was significantly decreased(F_(G0/G1)=85.878,F_s=56.455,F_(G2/M)=85.878,P<0.001).After 48 h,there was significant difference in SO-RB50 cell apoptosis rate among groups(F=11.312.P<0.05).The apoptosis rate in drug combination group was significantly higher than that of other groups(P<0.001).Condusion:ATRA can increase the sensitivity of SO-RB50 cells to vincristine.Vincristine combined with ATRA treatment can significantly increase the inhibiting effect on SO-RB50 cells,which may be related with promoting cell apoptosis and involving in cell cycle control.

Combined therapeutic effects of cytotoxic agent and differentiation-inducer on human gastric carcinoma cell line SGC-7901

Objective:To study the combined therapeutic effects of cytotoxic agent and differentiation-inducer on human gas tric carcinoma celll line SGC-7901 in vitro. Methods: The combined therapeutic effects of all-trans retinoic acid (ATRA), interferon a (IFNa)and fluorouracil (5-Fu) on gastric carcinoma cell line ax 7901 were observed when one of the 3, the combination of any 2 of the 3 and combination of all the 3 were administered respectively. The morphological and functional changes of gastric carcinoma cells were studied with MTT assay, flow cytometry, image analysis and determination of CEA content in the culture medium of the cells. Results: The cytostatic rate was increased as shown by the decrease of the rate of colony formation of the cells on culture disc when one agent, the combination of 2 agents and the combination of the 3 were administered progressively. The cells were relatively accumulated in the phase of G0/G1 and synthesis of DNA in he cells was inhibited.The malignant phenotype of the cells disappeared gradually while the characteristics of matUre cells were in creased. Meanwhile, CEA Level in the culture medium was decreased progressively. Apoptosis of the cells was oborved and a large amount of apoptotic apoptotic were found. Conclusion: The administration of the 3 agents in combination result in signif icant inhibition on proliferation, inducing of differention and promotion of apoptosis of gastric caxcinoma cells. The combina tion of cytotoxic agent and differention-inducer exerts significant inhibition on gastric carcinoma cells in vitro.

Central nervous system recurrence in a patient treated for acute promyelocytic leukemia,resulting in sideroblastic anemia:A case report

BACKGROUND Previous cases that have been stated in this article have displayed that around 1%to 7%of patients that have been treated with chemotherapy for acute promyelocytic leukemia developed myelodysplastic syndrome or acute myeloid leukemia.One can see that’s why this case presentation of a 60-year-old man that had a good response to acute promyelocytic leukemia treatment,that later presented with a central nervous system recurrence of acute promyelocytic leukemia and acquired sideroblastic anemia(a form of myelodysplasia)from treatment is a unique case report.CASE SUMMARY The presence of central nervous system relapse in acute promyelocytic leukemia patients is very unlikely compared to recurring mainly in the bone marrow.It is also uncommon to be diagnosed with sideroblastic anemia(form of myelodysplastic syndrome)as a result from treatment for acute promyelocytic leukemia.This case report highlights the detection,treatment/maintenance with idarubicin,all-trans-retinoic-acid,arsenic trioxide,methotrexate,6-mercaptopurine,and ommaya reservoir intrathecal methotrexate administration in a patient that had central nervous system relapse of acute promyelocytic leukemia and acquired sideroblastic anemia.CONCLUSION In essence,first time relapse concerning the central nervous system in treated acute promyelocytic leukemia patients who had a good response to therapy is very uncommon.The acquirement of a myelodysplastic syndrome such as ringed sideroblastic anemia is also rare regarding this patient population.Although such cases are infrequent,this case report represents a unique insight of the detection,treatment,and maintenance of a 60-year-old man diagnosed with acute promyelocytic leukemia,resulting in the acquirement of sideroblastic anemia and central nervous system relapse.