Cellular growth dynamics and allelopathic activity in coffee cell cultures were examined as follows: First, we compared allelopathic activity of seven woody plant calli, Coffea canephora, Derris indica, Ficus carica L...Cellular growth dynamics and allelopathic activity in coffee cell cultures were examined as follows: First, we compared allelopathic activity of seven woody plant calli, Coffea canephora, Derris indica, Ficus carica L., Juniperus conferta, Prunus persica, Punica granatum, and Sonneratia ovata, using a modified “sandwich method bioassay” and found that coffee callus showed the strongest growth inhibition to lettuce seedling nearly 90% of hypocotyl and 96% of root. This coffee callus actively proliferated, with a 21-fold increase during five weeks of subculture, with a growth curve comprising two typical phases: a lag phase of 0 - 2 weeks of culture and an exponential phase of 3 - 5 weeks of culture. Allelopathic activity varied depending on the growth phase of the coffee callus. The strongest allelopathic activity was detected in 1 - 2-week-old callus showing nearly 100% inhibitory effect on lettuce seedling growth. As the allelopathic activity of coffee calli is extremely high, beyond the natural level in coffee leaves and green beans, we focused on analyzing the allelopathic activity of its aqueous extracts using high-performance liquid chromatography. Several prominent peaks, including two reference alkaloids, theobromine and caffeine, which are known allelochemicals in coffee plants, and three distinct unknown peaks were identified at 270 nm in coffee calli during the lag phase (1 - 2 weeks of culture). The higher value of the total phenolic content in the lag phase also suggested a key biosynthetic pathway in relation to the allelopathic activity of coffee callus will be activated in the lag phase.展开更多
The allelopathic potential of aqueous and organic solvent extacts from Corrigiola telephiifolia Pour which is an aromatic and medicinal plant in mediterranean regions, was investigated under laboratory conditions on t...The allelopathic potential of aqueous and organic solvent extacts from Corrigiola telephiifolia Pour which is an aromatic and medicinal plant in mediterranean regions, was investigated under laboratory conditions on two plant species： Raphanus sativus and Triticum aestivum. The aqueous extract of Corrigiola telephiifolia roots had an important inhibitory effect on the seed germination of the two tested plant species. The dichloromethane and the methanol extracts significantly reduced the growth of radish roots up to 84.44% and 68.85% respectively. The butanol, the dichloromethane and the ethyl acetate extracts had a high inhibitory effect on seed germination and roots growth of wheat. The presence of saponins in butanol extracts and tanins in dichlorometane and ethyl acetate extracts may be responsible of these allelopathic activities. These results suggest that Corrigiola telephiifolia Pour have allelopathic effects and contains potent allelochemicals which should be used for weeds management.展开更多
Phenolic acids are very common compounds in pedosphere. Theobjective of this review was to summarize the current knowledge ofthe behaviors of phenolic acids in plant-soil microbe system. Whenphenolic acids originated ...Phenolic acids are very common compounds in pedosphere. Theobjective of this review was to summarize the current knowledge ofthe behaviors of phenolic acids in plant-soil microbe system. Whenphenolic acids originated form leaching, decomposition and exudationof living and dead plant tissues enter soils, they can reactphysiochemically with soil particle surfaces and/or incorporate intohumic matter. Phenolic acids desorbed from soil particle surfaces andremained in solution phase can be utilized by microbe as carbonsources and absorbed by plants.展开更多
文摘Cellular growth dynamics and allelopathic activity in coffee cell cultures were examined as follows: First, we compared allelopathic activity of seven woody plant calli, Coffea canephora, Derris indica, Ficus carica L., Juniperus conferta, Prunus persica, Punica granatum, and Sonneratia ovata, using a modified “sandwich method bioassay” and found that coffee callus showed the strongest growth inhibition to lettuce seedling nearly 90% of hypocotyl and 96% of root. This coffee callus actively proliferated, with a 21-fold increase during five weeks of subculture, with a growth curve comprising two typical phases: a lag phase of 0 - 2 weeks of culture and an exponential phase of 3 - 5 weeks of culture. Allelopathic activity varied depending on the growth phase of the coffee callus. The strongest allelopathic activity was detected in 1 - 2-week-old callus showing nearly 100% inhibitory effect on lettuce seedling growth. As the allelopathic activity of coffee calli is extremely high, beyond the natural level in coffee leaves and green beans, we focused on analyzing the allelopathic activity of its aqueous extracts using high-performance liquid chromatography. Several prominent peaks, including two reference alkaloids, theobromine and caffeine, which are known allelochemicals in coffee plants, and three distinct unknown peaks were identified at 270 nm in coffee calli during the lag phase (1 - 2 weeks of culture). The higher value of the total phenolic content in the lag phase also suggested a key biosynthetic pathway in relation to the allelopathic activity of coffee callus will be activated in the lag phase.
文摘The allelopathic potential of aqueous and organic solvent extacts from Corrigiola telephiifolia Pour which is an aromatic and medicinal plant in mediterranean regions, was investigated under laboratory conditions on two plant species： Raphanus sativus and Triticum aestivum. The aqueous extract of Corrigiola telephiifolia roots had an important inhibitory effect on the seed germination of the two tested plant species. The dichloromethane and the methanol extracts significantly reduced the growth of radish roots up to 84.44% and 68.85% respectively. The butanol, the dichloromethane and the ethyl acetate extracts had a high inhibitory effect on seed germination and roots growth of wheat. The presence of saponins in butanol extracts and tanins in dichlorometane and ethyl acetate extracts may be responsible of these allelopathic activities. These results suggest that Corrigiola telephiifolia Pour have allelopathic effects and contains potent allelochemicals which should be used for weeds management.
基金Project (No. 5015103) supported by the Laboratory of Material Cycling in Pedosphere, the Chinese Academy of Sciences.
文摘Phenolic acids are very common compounds in pedosphere. Theobjective of this review was to summarize the current knowledge ofthe behaviors of phenolic acids in plant-soil microbe system. Whenphenolic acids originated form leaching, decomposition and exudationof living and dead plant tissues enter soils, they can reactphysiochemically with soil particle surfaces and/or incorporate intohumic matter. Phenolic acids desorbed from soil particle surfaces andremained in solution phase can be utilized by microbe as carbonsources and absorbed by plants.
