Study on Cytokines IL-2,IL-6,IL-10 in Patients of Chronic Allergic Rhinitis Treated with Acupuncture

OBJECTIVES: To observe the plasmatic concentration of IL-6, IL-10 and IL-2 in the patient of chronic allergic rhinitis before and after acupuncture therapy. METHODS: Cytokine levels were determined before and after treatment in 30 healthy volunteers (Group A) and 90 patients of chronic allergic rhinitis (Group B) with an increased plasma IL-10 level. Group B was then divided into 3 subgroups: 30 patients treated with real acupuncture (Group B1); 30 patients treated with sham acupuncture (Group B2); 30 non-treated patients (Group B3). RESULTS: The allergic subjects of group B1, compared with controls, showed a significant reduction of IL-10 after a specific treatment with acupuncture (P

A CLINICAL OBSERVATION ON THERAPEUTIC EFFECTS OF ACUPUNCTURE FOR ALLERGIC RHINITIS

The author applied acupuncture to treat52 cases of allergic rhinitis with certaintherapeutic effectiveness confirmed by afollowing-up for one year and more.The resultis reported as follows.

Mechanism of Magnoliae Flos and Xanthii Fructus herb pair in treatment of allergic rhinitis based on network pharmacology

Drug Combination Therapy has retracted this article because the data is falsified,the core targets are wrong and this English article has a high repetition rate compared with a Chinese article.Drug Combination Therapy received the author’s manuscript on 13 April 2022.After the plagiarism check on 13 April 2022,the similarity rate of this article was 15%(Supplementary 1),which was qualified.Then the manuscript was started to be sent for peer-review at 10:10 on 19 April 2022.

Treatment and Prevention of 100 Cases with Allergic Rhinitis by Acupoint Sticking Therapy

Objective: To observe the clinical effects of acupoint sticking therapy in the three ten-day periods in summer and three nine-day periods in winter. Methods: Herbal medicines were prepared into crude herbal powder and made into paste with ginger juice, and were respectively applied to the corresponding acupoints in the three ten-day periods in summer and three nine-day periods in winter. Results: The results showed 45 cases were cured, 50 cases were improved, and 5 cases failed out of 100 cases, with the total effective rate of 95%. Conclusion: The acupoint sticking therapy for the treatment and prevention of allergic rhinitis is simple in method, remarkable in effects, acceptable to the patients, and is worthy of clinical popularization.

Analysis of the Effect of Combined DesensitizingAcupoints Therapy in Treating 419 Cases of Allergic Rhinitis Accompanied Asthma

Objective: To explore the effect of combined acupoint desensitizing therapy.Methods: A total of 419 cases of allergic rhinitis accompanied with asthma were treated by combined acupoint desensitizing therapy with positive allergen extract on acupoints of head and upper back region.Results: After 3 courses of treatment, skin test repeated with responding allergen extract showed that the scope of redness and swelling of skin were smaller than those before treatment,P < 0.01. The3H-TdR incorporation lymphocyte transformation rate, acidophil count, IgA, IgG level and E-rosette formation rate of acupoint desensitizing group were different significantly from those of the two control groups (the acupoint 0.85% normal saline injection group and the subcutaneous desensitizing group),P < 0.01 orP < 0.05. Follow-up study for over 3 years showed that the markedly effective rate of the acupoint desensitizing group was 68.73%, the effective rate 29.12% and total effective rate, 97.85%. The effect was higher than that of the two control groups, P<0.01.Conclusion: Combined acupoint desensitizing therapy is valuable and worthy of popularizing in treating patients of allergic rhinitis accompanied asthma.

Observation on Therapeutic Effects of Cupping Therapy for Allergic Rhinitis

Allergic rhinitis is mainly characterized by the symptoms of sneezing, tickling sensation in the nose, runny nose, and nasal obstruction, and often occurs after invasion of pathogenic wind and cold. The author has treated 50 cases of this disease by applying cupping therapy on Shenque （CV 8）. Now, the report is given as follows.

Acupuncture for allergic rhinitis: a systematic review and meta analysis

Objective： To analyze and review the clinical efficacy of acupuncture（including electroacupuncture） alone for allergic rhinitis（AR） and to compare its efficacy with antihistamines and Chinese patent medicine Bi Yan Kang Tablet. Methods： The search strategy, inclusion and exclusion criteria were made according to the principle of evidence-based medicine. We performed a systematic search on China National Knowledge Infrastructure（CNKI）, Wanfang Academic Journal Full-text Database（Wanfang）, Chongqing VIP Database（CQVIP）, Chinese Biomedical Literature Database（CBM）, Pub Med, Excerpta Medica Database（EMBASE）, Web of Science, Cochrane Library, and Cochrane Central Register of Controlled Trials（CENTRAL） for randomized controlled trials（RCTs） of acupuncture for allergic rhinitis between January 1990 and December 2015. The quality was evaluated by Cochrane Handbook for Systematic Reviews of Interventions Version 5.1, and the meta-analysis was conducted by Rev Man 5.3 version. Results： Twenty eligible RCTs were included into the meta-analysis after selection. Compared with antihistamines, the meta-analysis showed RR=1.241, 95%CI[1.15, 1.33], P〈0.00001, indicating that acupuncture achieved a better total effective rate for AR than antihistamines; MD=–0.93〈0, 95%CI[–1.22, –0.63], P〈0.00001, indicating that acupuncture is better than antihistamines in decreasing the total nasal symptom score（TNSS） in AR patients; and MD=1.46〉0, 95%CI[–10.84, 13.75], P=0.82, indicating that there was no statistical difference between acupuncture and antihistamines in regulating immunoglobulin E（Ig E） in AR patients. Compared with Bi Yan Kang Tablet, the meta-analysis has shown RR=1.50〉1, 95%CI[1.30, 1.73], P〈0.00001, indicating that acupuncture achieved a better total effective rate for AR than Chinese patent medicine Bi Yan Kang Tablet. Conclusion： Acupuncture alone can achieve a better total effective rate for AR than antihistamines and Bi Yan Kang Tablet. It is also better than antihistamines in improving clinical symptom scores; however, whether acupuncture is better than Bi Yan Kang Tablet needs further proof. As far as current data are concerned, there was no statistical difference between acupuncture and antihistamines in improving serum Ig E; further study is needed in this regard. The risk of bias due to absent randomization methods or blinding implementation decreased the evidence level of the overall conclusion.

Intranasal application of Epstein-Barr virus/lipoplex to abrogate eosinophillia in murine model of allergic rhinitis

Background Currently anti-inflammatory therapy with steroids for allergic rhinitis need long-term repeated administration, although it is effective. Gene therapy is being suggested to substitute it. The aim of this study was to investigate nonviral vector mediated exogenous gene expression in COS-7 cells in vitro and the effect of intranasal mouse interleukin （mIL）-12 transgene expression on allergen induced eosinophil infiltration of nasal mucosa in a murine model of allergic rhinitis. Methods In vitro COS-7 cells were infected with Epstein-Barr virus （EBV）/lipoplex. The expression of IL-12 p70 in cell culture supernatant was examined by enzyme-linked immunosorbent assay （ELISA）. In mice with ovalbumin （OVA） induced allergic rhinitis, EBV/lipoplex was administered by nasal drops before OVA challenge once a day from day 1 to day 10. The expression of IL-12 mRNA and protein, the change of eosinophil count in nasal mucosa and serum total IgE were measured 24 hours after the last challenge. Results EBV/lipoplex could effectively transfect COS-7 cells. The expression of IL-12 p70 in cell culture supernatant was significantly more than in blank control. IL-12 via EBV plasmid vector transduction could be overexpressed in vivo. In pGEGmIL-12 treated models, the nasal mucosa revealed a high level of widespread raiL-12 transduction by immunohistochemistry and in situ hybridization. Histological evaluation revealed marked suppression of eosinophil infiltration in nasal mucosa. The eosinophil count in allergic rhinitis group [（26.5±9.8）/high-power field （HPF）] was significantly increased over control group [（0.40±0.52）/HPF] （F=56.94, P〈0.01）, while the count in IL-12 gene therapy group [（4.60±2.63）/HPF] was significantly less than that of allergic group （F=56.9, P〈0.01）. Serum total IgE between in gene therapy mice [（88.83±6.71） ng/ml] and allergic rhinitis mice [（103.1±5.7） ng/ml] showed a significant difference （F= 1216, P〈0.05）. Conclusions Nonviral EBV plasmid vector, pGEGmIL-12 was able to overexpress exogenous gene both in vitro and in murine nasal mucosa in vivo. IL-12 overexpression via EBV/lipoplex could stem allergen induced eosinophil infiltration in nasal mucosa in murine models of allergic rhinitis, which may suggest a new cytokine immunogenetic therapy for allergic rhinitis.

Influencing factors on efficacy of summer acupoint application treatment for allergic rhinitis:a retrospective study

OBJECTIVE:Allergic rhinitis(AR) is a common health problem.Summer acupoint application treatment(SAAT) is reported to effectively treat and prevent AR from seasonal onset.In the present study,we aimed to evaluate its effects,especially on the course of AR,through a retrospective study.METHOD:A cross-sectional multicenter study was performed based on patients treated between 2008 and 2009 in 13 clinical centers in China.A total of 1058 outpatients aged ≥2 years with documented AR and ≥1 year SAAT were eligible for enrollment.A case report form(CRF) was completed by both patient and doctor.The CRF was designed to collect data on the patient's history of SAAT,AR condition,and self-reported health condition.The outcomes(dependent variables) were incidence and intensity of AR and concomitant medications used.Data were analyzed with ordinal logistic regression(OLR).RESULTS:Treatment course and seasonal pattern of AR were related to all dependent variables positively.After controlling for sample bias and confounding factors,the findings suggested that a 3-year treatment course had better efficacy(OR/incidence of AR:2.57,95%CI:1.76-3.76;OR/intensity of AR:2.17,95%CI:1.50-3.17;OR/concomitant medications:2.20,95% CI:1.50-3.23) compared with a 2-year or less treatment course.CONCLUSION:The results showed that:1)the length of treatment course was positively associated with the efficacy of SAAT(the longer the treatment course,the better the efficacy);and 2)SAAT was more efficacious in treating seasonal AR than non-seasonal AR.

rherapeutic efficacy observation on acupuncture for persistent allergic rhinitis

Objective： To observe the clinical effect of acupuncture for persistent allergic rhinitis （PAR）. Methods： A total of 154 PAR patients were randomized into a treatment group and a control group, 78 cases in the treatment group received acupuncture at meridian points, while 76 cases in the control group received acupuncture at non-meridian points. The treatment was done every other day, 3 times a week for a total 4 weeks. The total nasal symptom score （TNSS）, the total non-nasal symptom score （TNNSS） and the rhinoconjunctivitis quality of life questionnaire （RO, LQ） were measured before the treatment and after 2 weeks and 4 weeks of treatment, as well as 1 month and 3 months after the treatment to compare the clinical effect between the two groups. Results： After 4 weeks of treatment, the total effective rate in the treatment group was 92.3%, versus 76.3% in the control group, showing a statistically significant difference （P〈0.05）. Intra-group comparisons of TNSS, TNNSS and R0.L0. scores after 4 weeks of treatment showed statistically significant differences （all P〈0.05）. Between-group comparisons of TNSS, TNNSS and RQLQ score after 4 weeks of treatment and in follow-up visits showed statistically significant differences （all P〈0.05）. Conclusion： Acupuncture is effective for PAR, and acupuncture at meridian points can produce a better effect than acupuncture at non-meridian points.

Exploration of Clinical Regularities in Acupuncture and Moxibustion Treatment for Allergic Rhinitis

Objective： To review literature from 1956 to 2007 and explore regularities in points selection and methods for acupuncture-moxibustion treatment of allergic rhinitis. Methods： A comprehensive analysis was made by a method of metrological analysis according to the data from ＂China Modem Acupuncture-moxibustion Information Databank＂. Results： Points Yingxiang （LI 20） and Feishu （BL 13） were most frequently used. Acupuncture and hydro-acupuncture were the major treatment methods. Conclusion： It is suggested that Yingxiang （LI 20）, Yintang （Ex-HN 3）, Feishu （BL 13） and Dazhui （GV 14） be used as a basic prescription for acupuncture-moxibustion treatment of allergic rhinitis, which is combined with point selection based on syndrome differentiation.

Treatment of 31 Cases of Allergic Rhinitis with Acupuncture plus Moving Cupping

Objective： To observe the clinical efficacy of acupuncture and cupping on the back in the treatment of allergic rhinitis. Methods： Thirty-one cases of allergic rhinitis were treated by acupuncture and moving cupping, twice a week; five treatments made up one course and two-course treatments were given. Result： Sixteen cases were cured, 14 cases improved and 1 case failed. Conclusion： Acupuncture plus cupping has positive effects on allergic rhinitis.

Clinical Observation of Warm Needling Moxibustion for Seasonal Allergic Rhinitis

Objective： To observe the clinical therapeutic effects of warm needling moxibustion for seasonal allergic rhinitis. Methods： One hundred and eighty cases of the patients with seasonal allergic rhinitis were randomly divided into two groups, 90 cases in the warm needling group, treated by acupuncture plus warm needling moxibustion, and 90 cases in the acupuncture group, treated by acupuncture only, to observe the therapeutic effects after one course of the treatments. Results： The total effective rate was 95.6% in the warm needling group and 85.6% in the acupuncture group, with significant statistical difference in comparison between the two groups. Conclusion： The total effective rate of the warm needling moxibustion is better than that of the acupuncture treatment in the treatment of seasonal allergic rhinitis.

Therapeutic observation on acupuncture-moxibustion at different intervals forpersistent allergic rhinitis

Objective:To observe the therapeutic effect of acupuncture-moxibustion at different intervals on persistent allergic rhinitis.Methods:A total of 90 patie nts con forming to the inclusi on criteria were ran domized into three groups n amed A,B and C by randomized block method.Patients in all tMee groups received the same treatment of acupuncture and herbal cake-partitioned moxibustion at the same acupoints,while the treatment frequency was different.Patients in group A received the treatment once a week,group B twice a week and group C three times a week,and all the treatment lasted for 4 weeks.The total nasal symptom score(TNSS),total ocular symptom score(TOSS)and Sino-nasal outcome test-20(SNOT-20)were evaluated before and after treatment.The self-rating score of symptoms was evaluated during treatment and 2 weeks after treatment.Results:The total effective rate was 80.0%in group A,93.3%in group B,and 100.0%in group C.The total effective rate in group A was statistically different from that in group B and group C(both P<0.05),but there was no significant inter-group difference in total effective rate between group B and group C(P>0.05).After treatment,scores of TNSS,TOSS and SNOT-20 in all three groups dropped significantly,and stat:istically different from those before treatment(all P<0.05);between-group comparison showed group B and group C decreased more obvious than group A(all P<0.05).In self-rating score of symptoms,there were no inter-group statistically significant differences in the first 3-week treatment(all P>0.05);after 4-week treatment,the score in group A was higher than that in group B and group C,and showed statistical significant(both P<0.05);at 2 weeks after treatment,the score in group A was higher than that in group B and group C based on an everyday record,showing statistical significanee(both P<0.05).At the 11th day after treatment,the score in group B was higher than that in group C(P<0.05).There were no significant differences between group B and group C at other time points(all P>0.05).Conclusion:All three protocols are effective for allergic rhin itis.With the in crease of treatme nt freque ncy,the therapeutic efficacy with a treatment frequency of twice a week and three times a week is superior to that of once a week.Frequency of three times a week has a better long-term effect than once and twice a week,together with the least fluctuation of symptoms.

Treatment of 82 Cases of Allergic Rhinitis with Aconite Cake-partitioned Moxibustion

Allergic rhinitis is mainly characterized by recurrent sneezing and runny nose. The author has treated 82 cases of allergic rhinitis with aconite cake-partitioned moxibustion and now it is reported as follows.

Mild moxibustion plus loratadine tablets for children with allergic rhinitis:a randomized controlled trial

Objective To explore the efficacy of mild moxibustion plus loratadine tablets for children with allergic rhinitis(AR).Methods A total of 80 children were randomized into a control group and an observation group,with 40 cases in each group.The control group was treated with loratadine tablets,and the observation group was treated with mild moxibustion plus loratadine tablets.Before and after treatment,the total nasal symptom score(TNSS)was evaluated,and the serum eosinophils(EOS)count,and the interleukin(IL)-27 and macrophage migration inhibitory factor(MIF)levels were measured.Clinical efficacy was evaluated after treatment.Results The total effective rate of the observation group was higher than that of the control group(P<0.05).After treatment,the TNSS in both groups decreased(P<0.05),and the TNSS in the observation group was lower than that in the control group(P<0.05);the serum EOS count in both groups decreased(P<0.05),and the serum EOS count in the observation group was lower than that in the control group(P<0.05).The serum IL-27 level in the control group had no statistical difference compared with the same group before treatment(P>0.05),and the serum MIF level decreased after treatment(P<0.05).The serum IL-27 level in the observation group increased after treatment(P<0.05),and the serum MIF level decreased after treatment(P<0.05),and were both statistically different from those in the control group(P<0.05).Conclusion Mild moxibustion plus loratadine tablets is effective in treating children with AR.It can significantly improve the nasal symptoms and reduce the serum EOS count,which may be related to the regulation of the serum IL-27 and MIF levels.

Clinical study on Jin's three-needle plus Xiang Ju Capsules for allergic rhinitis

Objective：To observe the clinical effects of Jin＇s three-needle plus Xiang Ju Capsules for allergic rhinitis（AR）.Methods：Fifty patients with AR were randomly divided into an acupuncture-medication group and a medication group by the random digital table,25 cases in each group.The acupuncture-medication group was treated with dorsal three-needle and nasal three-needle in Jin＇s three-needle therapy,once every day,plus oral administration of Xiang Ju Capsules,4 pills each time,and three times per day.The medication group was only treated with oral administration of Xiang Ju Capsules,4 pills each time,and three times per day.For both groups,10-day treatment constituted a course.The therapeutic effects were observed after continuous 2 courses.The symptoms and signs are scored before and after treatment,and the therapeutic effects were assessed based upon the changes of the patient＇s symptoms and signs.Results：After treatment,the total effective rate was 96.0% in the acupuncture-medication group and 72.0% in the medication group.The difference in the total effective rate between the two groups was statistically significant（P〈0.05）.Conclusion：The therapeutic effect was remarkable in treating AR of Jin＇s three-needle plus Xiang Ju Capsules,better than oral administration of Xiang Ju Capsules alone.

Treatment of 48 Cases of Perennial Allergic Rhinitis with Thunder-fire Moxibustion

Perennial allergic rhinitis is an allergic reaction of the nasal mucosa. It is characterized by paroxysmal and repeated runny nose, sneeze, stuffy nose, rhinocnesmus and nasal mucosa edema. The author used ＂Doctor ZHAO＇s thunder-fire moxibustion＂ in treating 48 cases of this disease. Now it is reported as follows.

Clinical observation of Zhen'ai needling method in Nei Jing (Classic of Internal Medicine) on improving quality of life in patients with allergic rhinitis

Objective:To observe the effect of Zhen'ai needling method in Nei Jing (Classic of Internal Medicine) on quality of life in patients with allergic rhinitis (AR).Methods:A total of 99 patients with AR who met the inclusion criteria were randomized into a conventional needling group (51 cases) and a Zhen'ai needling group (48 cases).The conventional needling group was treated with acupuncture at Zusanli (ST 36),Hegu (LI 4),Juliao (ST 3),Yingxiang (LI 20),Yintang (GV 29),Shangxing (GV 23) and Baihui (GV 20).The Zhen'ai needling group added acupoints of Zhen'ai needling method {Lieque (LU 7),Shanglianquan [Extra,located at 1 cun above Lianquan (CV 23)] and Tianrong (SI 17)} in addition to points in the conventional needling group.The needles were retained for 30 min.The treatment was performed twice a week.All patients were assessed by total nasal symptom score (TNSS),total ocular symptom score (TOSS) and sino-nasal outcome test-20 (SNOT-20) before and after 15 treatments to evaluate the quality of life.The clinical efficacy was evaluated after 15 treatments.Results:During the treatment,8 cases dropped out in the conventional needling group and 7 cases in the Zhen'ai needling group.There were no significant differences in TNSS,TOSS and SNOT-20 scores between the two groups before treatment (all P＞0.05).After treatment,the total effective rate of the Zhen'ai needling group was 92.7％,versus 88.4％ in the conventional needling group.There was no significant difference between the two groups (P＞0.05).After treatment,there were no significant differences between the two groups in TNSS and TOSS scores (both P＞0.05).The SNOT-20 score of the Zhen'ai needling group was lower than that of the conventional needling group,and the difference was statistically significant (P＜0.05).There were statistical differences between the two groups in the scores of cough and nasal reflux (to the throat) of the NOT-20 scale (both P＜0.05).Conclusion:Both Zhen'ai needling method and conventional acupuncture are effective in the treatment of AR.Zhen'ai needling method has a better effect than conventional acupuncture in improving cough and nasal reflux (to the throat) in AR patients.

Observation on Clinical Effect of Acupuncture for Allergic Rhinitis

Objective:To observe the clinical effect of acupuncture plus acupoint injection and laser radiation on the nasal cavity for allergic rhinitis.Methods:Ninety patients with allergic rhinitis were randomly divided into a treatment group and a control group,45 cases in each group.The treatment group was treated by acupuncture,acupoint injection method and He-Ne laser radiation on the nasal cavity.The control group was treated by Triamcinolone Acetonide nasal spray.The clinical effects were assessed after two courses of treatment.Results:The clinical curative rate was 88.9%and the total effective rate was 100.0%in the treatment group,versus 57.8%and 80.0%in the control group.The clinical curative rate and total effective rate were remarkably higher in the treatment group than those in the control group(P<0.05).In comparison of the therapeutic effects in the different courses between the two groups,the curative rates in the one course and two courses were higher in the treatment group than those in the control group(P<0.05).In the follow-up visit of the cured patients of the two groups for half a year,the recurrence rate was 2.5%in the treatment group versus 34.6%in the control group,with a statistical difference between the two groups(P<0.01).Conclusion:Acupuncture plus acupoint injection and laser radiation on the nasal cavity for allergic rhinitis has better clinical effect and long-term effect,and can obviously shorten the course,enhance the clinical effect and reduce the recurrence rate.