Allexivirus属的一个新成员——大蒜病毒E的基因组全序列测定和系统树分析

测定了一个从浙江余杭大蒜中分离到的Allexivirus属成员的基因组全序列. 单链正性病毒基因组含8451个核苷酸, 6个ORF. 与其他Allexivirus属成员的基因组全序列的核苷酸同源性仅为62.8%～64.8%; ORF1～ORF6编码蛋白的氨基酸同源性分别为67.6%～78.5%, 55.4%～66.2%, 56.7%～66.4%, 40.3%～55.6%, 66.3%～79.7%和52.2%～68.8%. 进一步分析显示, 此病毒与其他病毒的差异和该属中远缘病毒间的差异相似, 外壳蛋白核心区域氨基酸序列同源性仅为63.9%～79.8%, 远低于该属不同病毒的国际分类标准, 应归类为Allexivirus属的新成员, 命名为大蒜病毒E. 病毒不同编码蛋白序列的进化树分析也进一步证明了该病毒与其他病毒的亲缘关系.

Genome organization and phylogenetic tree analysis of Garlic virus E,a new member of genus Allexivirus

The complete sequence of an Allexivirus iso-lated from garlic plants in Yuhang City, Zhejiang Province, China had been determined. The single-strand, positive RNA genome was 8451 nucleotides in length excluding poly(A) tail. The genome organization of this virus was similar to that of the other Allexiviruses but only with 62.8%-64.8% nucleo-tide acid identities. The amino acid sequences of proteins encoded by ORFl-6 shared 67.6%-78.5%, 55.4%-66.2%, 56.7%-66.4%,40.3%-55.6%,66.3%-79.7%and 52.2%-68.8% identities with those of the others respectively. The homology range between it and the other Allexiviruses was similar to that between the other distinct species in this genus. A more comprehensive comparison using all available CP amino acid sequences showed that it shared only 63.9%-79.8% amino acids identical with the others. Therefore, it had been considered as a new member of the genus, named as garlic virus E (GarV-E). Phylogenetic analysis confirmed GarV-E as a distinct member and the correct names