Repair of peripheral nerve defects with chemically extracted acellular nerve allografts loaded with neurotrophic factors-transfected bone marrow mesenchymal stem cells

Chemically extracted acellular nerve allografts loaded with brain-derived neurotrophic fac- tor-transfected or ciliary neurotrophic factor-transfected bone marrow mesenchymal stem cells have been shown to repair sciatic nerve injury better than chemically extracted acellular nerve allografts alone, or chemically extracted acellular nerve allografts loaded with bone marrow mesenchymal stem cells. We hypothesized that these allografts compounded with both brain-derived neurotrophic factor- and ciliary neurotrophic factor-transfected bone marrow mesenchymal stem cells may demonstrate even better effects in the repair of peripheral nerve injury. We cultured bone marrow mesenchymal stem cells expressing brain-derived neuro- trophic factor and/or ciliary neurotrophic factor and used them to treat sciatic nerve injury in rats. We observed an increase in sciatic functional index, triceps wet weight recovery rate, myelin thickness, number of myelinated nerve fibers, amplitude of motor-evoked potentials and nerve conduction velocity, and a shortened latency of motor-evoked potentials when al- lografts loaded with both neurotrophic factors were used, compared with allografts loaded with just one factor. Thus, the combination of both brain-derived neurotrophic factor and cili- ary neurotrophic factor-transfected bone marrow mesenchymal stem cells can greatly improve nerve injury.

大段同种骨移植5年后骨标本的病理表现

目的通过对1例骨肿瘤保肢术5年后因骨折取出的同种骨标本的病理学分析,探讨大段同种骨移植后在体内的修复过程。方法6岁女孩股骨骨肉瘤切除,行深冻辐照同种股骨干移植,长度20cm,术后骨愈合良好,5年后因意外致植入骨中下段斜劈形骨折,手术取出部分骨折片,进行光镜和扫描电镜观察,取未植入的同种骨材料作为对照。结果植入骨外观与对照相似;植入骨内的部分管腔扩大、形态不规则,骨表面及扩大的哈氏管表面见薄层新骨,黏合线清晰,哈氏管附近骨陷窝内见骨细胞;骨折面大量吸收窝,可见破骨细胞和成骨细胞。结论植入骨内已出现再血管化和一定程度的骨吸收及新骨形成。

异体骨修复骨关节缺损后腱-骨固定和愈合的研究进展

目的：探讨异体骨修复骨关节缺损的愈合机理及腱-骨固定和促进腱．骨愈合的进展。方法：查阅相关中外文献，分析异体骨修复骨关节缺损的愈合过程及腱-骨固定和愈合的进展。结果：骨传导骨诱导作用修复骨关节缺损，腱-骨固定的方法各有优缺点。结论：异体骨植入后腱-骨愈合与自体骨的腱-骨愈合过程相似，有诸多因素促进腱-骨愈合的发展。