We developed and implemented a methodology that allowed extracting and evaluating high molecular weight polysaccharides present in the gel of Aloe barbadensis Miller. One of the fractions evaluated revealed the presen...We developed and implemented a methodology that allowed extracting and evaluating high molecular weight polysaccharides present in the gel of Aloe barbadensis Miller. One of the fractions evaluated revealed the presence of high molecular weight carbohydrates (200 kDa) with a behavior similar to that of acemannan and another fraction with compounds of molecular weights between 17 and 47 kDa. We quantified the concentration of acemannan for two different growing periods. The concentration of acemannan in the high molecular weight fraction was 99.97 ppm in the rainy season and 106.03 ppm in the dry season. The concentration of acemannan in the fraction of low molecular weight was 9.364 ppm during the season of greatest rainfall and 26.939 ppm in the dry season.展开更多
Diabetes is a chronic disease that requires a long term management where oxidative stress plays a pivotal role in disease progression and intensifying secondary complications. In spite of all the research on diabetes ...Diabetes is a chronic disease that requires a long term management where oxidative stress plays a pivotal role in disease progression and intensifying secondary complications. In spite of all the research on diabetes and recent advances in diabetes treatments, the reality is that there is no cure for diabetes and its devastating complications. While currently available anti-diabetic therapies are effective in reducing blood glucose level, they are not without associated side effects when they are used for a long term applications. As a result, physicians and patients are inclining more towards to a safer therapy with less serious side effects in the form of medicinal foods and botanical alternatives that are suitable for chronic usage. Aloesin, an Aloe chromone, has previously been formulated with an aloe polysaccharide to give a composition called Loesyn, where it showed significant impact in reducing glycosylated hemoglobin, fasting blood glucose, fructosamine and plasma insulin level in humans. Radical scavenging activities of chromones and polysaccharides from Aloe have also been reported. Here we rationalize the relevance of use of Aloesin alone or in a standardized blend with Aloe polysaccharides, as a potential medical food to manage systemic oxidative stress and/or high blood glucose of diabetes.展开更多
文摘We developed and implemented a methodology that allowed extracting and evaluating high molecular weight polysaccharides present in the gel of Aloe barbadensis Miller. One of the fractions evaluated revealed the presence of high molecular weight carbohydrates (200 kDa) with a behavior similar to that of acemannan and another fraction with compounds of molecular weights between 17 and 47 kDa. We quantified the concentration of acemannan for two different growing periods. The concentration of acemannan in the high molecular weight fraction was 99.97 ppm in the rainy season and 106.03 ppm in the dry season. The concentration of acemannan in the fraction of low molecular weight was 9.364 ppm during the season of greatest rainfall and 26.939 ppm in the dry season.
文摘Diabetes is a chronic disease that requires a long term management where oxidative stress plays a pivotal role in disease progression and intensifying secondary complications. In spite of all the research on diabetes and recent advances in diabetes treatments, the reality is that there is no cure for diabetes and its devastating complications. While currently available anti-diabetic therapies are effective in reducing blood glucose level, they are not without associated side effects when they are used for a long term applications. As a result, physicians and patients are inclining more towards to a safer therapy with less serious side effects in the form of medicinal foods and botanical alternatives that are suitable for chronic usage. Aloesin, an Aloe chromone, has previously been formulated with an aloe polysaccharide to give a composition called Loesyn, where it showed significant impact in reducing glycosylated hemoglobin, fasting blood glucose, fructosamine and plasma insulin level in humans. Radical scavenging activities of chromones and polysaccharides from Aloe have also been reported. Here we rationalize the relevance of use of Aloesin alone or in a standardized blend with Aloe polysaccharides, as a potential medical food to manage systemic oxidative stress and/or high blood glucose of diabetes.
文摘目的:研究芦荟多糖的提取、纯化工艺及体外抗肿瘤活性,为芦荟的产业化开发提供实验依据。方法:采用水提醇沉,三氯乙酸法除蛋白,DEAE-52离子交换色谱分离。用Sephacryl S-400凝胶柱层析纯化并确定芦荟多糖的相对分子量,HPLC法检测纯度,GC法鉴别单糖组成及分子摩尔比。以MTT法观察其体外抗肿瘤活性等。结果:从中华芦荟(Aloe vera L.var. chinesis(Haw.)Berger.)凝胶冻干粉中提取分离得到纯多糖AVP,经HPLC检测为单一对称峰,相对分子量为86000,由甘露糖和葡萄糖组成,摩尔比为10.3∶1.0。AVP对人肝癌细胞(SMMC-7721)、人胃癌细胞(BGC-823)及人肺癌细胞(A549)的增殖均有抑制作用。结论:该法分离的芦荟多糖AVP纯度好,收率高达3.6%,可用于进一步的研究和开发,提高芦荟的资源利用率。
