Ecological Restoration Technology and Carbon Reduction Paths of Abandoned Mines in Zoige Alpine Grassland Area

At first,the ecological restoration technology of abandoned mines is summarized,and the paths and models of mine ecological restoration in Zoige grassland area under the background of carbon neutrality are analyzed.Moreover,the problems and deficiencies in the current research on mine ecological restoration in Zoige grassland area are initially identified,and the future research trend is prospected to provide a reference for the path of carbon reduction by mine ecological restoration in Zoige grassland area and other alpine grassland areas in the future.

Volume fractal dimension of soil particles and relationships with soil physical-chemical properties and plant species diversity in an alpine grassland under different disturbance degrees

Fractal geometry is an important method in soil science,and many studies have used fractal theory to examine soil properties and the relationships with other eco-environmental factors.However,there have been few studies examining soil particle volume fractal dimension in alpine grasslands.To study the volume fractal dimension of soil particles （D） and its relationships with soil salt,soil nutrient and plant species diversity,we conducted an experiment on an alpine grassland under different disturbance degrees：non-disturbance （N0）,light disturbance （L）,moderate disturbance （M） and heavy disturbance （H）.The results showed that （1） Ds varied from 2.573 to 2.635 among the different disturbance degrees and increased with increasing degrees of disturbance.（2） Shannon-Wiener diversity index,Pielou＇s evenness index and Margalef richness index reached their highest values at the M degree,indicating that moderate disturbance is beneficial to the increase of plant species diversity.（3） In the L and M degrees,there was a significant positive correlation between D and clay content and a significant negative correlation between D and soil organic matter （SOM）.In the H degree,D was significantly and positively correlated with total salt （TS）.The results suggested that to a certain extent,D can be used to characterize the uniformity of soil texture in addition to soil fertility characteristics.（4） For the L degree,there was a significant negative correlation between D and the Shannon-Wiener diversity index; while for the M degree,there was a significant negative correlation between D and Pielou＇s evenness index.

Fluxes of CO_2,CH_4 and N_2O from alpine grassland in the Tibetan Plateau

Using stat ic chamber technique, fluxes of CO 2 , CH 4 and N 2 O were measured in the alpine grassland area from July 2000 to July 2001, d eterminations of mean fluxes showed that CO 2 and N 2 O were gene rally released from the soil, while the alpine grassland accounted for a weak CH 4 sink. Fluxes of CO 2 , CH 4 and N 2 O ranged widely. The highest CO 2 emission occurred in August, whereas a lmost 90% of the whole year emission occurred in the growing season. But the variations of CH 4 and N 2 O fluxes did not show any clear patterns over the one-year-experim ent. During a daily variation, the maximum CO 2 emission occurred at 16:00, and then decreased to the minimum emi ssion in the early morning. Daily pattern analyses indicated that the variation in CO 2 fluxes was positively related to air temperatures (R 2 =0.73) and soil temperatures at a depth of 5 cm (R 2 =0.86), whereas daily variations in CH 4 and N 2 O fluxes were poorly explained by soil temperatures and climatic va riables. CO 2 emissions in this area were much lower than other grasslands in plain areas .

Fluxes of methane,carbon dioxide and nitrous oxide in an alpine wetland and an alpine grassland of the Tianshan Mountains,China

Methane （OH4）, carbon dioxide （CO2） and nitrous oxide （N2O） are known to be major greenhouse gases that contribute to global warming. To identify the flux dynamics of these greenhouse gases is, therefore, of great significance. In this paper, we conducted a comparative study on an alpine grassland and alpine wetland at the Bayinbuluk Grassland Eco-system Research Station, Chinese Academy of Sciences. By using opaque, static, manual stainless steel chambers and gas chromatography, we measured the fluxes of CH4, N2O and CO2 from the grassland and wetland through an in situ monitoring study from May 2010 to October 2012. The mean flux rates of CH4, N2O and CO2 for the experimental alpine wetland in the growing season （from May to October） were estimated at 322.4 μg/（m2.h）, 16.7 μg/（m2.h） and 76.7 mg/（m2.h）, respectively; and the values for the alpine grassland were -88.2 μg/（m2.h）, 12.7 μg/（m2.h）, 57.3 mg/（m2.h）, respectively. The gas fluxes showed large seasonal and annual variations, suggesting weak fluxes in the non-growing season. The relationships between these gas fluxes and environmental factors were analyzed for the two alpine ecosystems. The results showed that air temperature, precipitation, soil temperature and soil moisture can greatly influence the fluxes of CH4, N2O and CO2, but the alpine grassland and alpine wetland showed different feedback mechanisms under the same climate and environmental conditions.

Soil microbial community composition and its driving factors in alpine grasslands along a mountain elevational gradient

Understanding the vertical distribution patterns of soil microbial community and its driving factors in alpine grasslands in the humid regions of the Tibet Plateau might be of great significance for predicting the soil microbial community of this type of vegetation in response to environmental change. Using phospholipid fatty acids （PLFA）, we investigated soil microbial community composition along an elevational gradient （3094-4131 m above sea level） on Mount Yajiageng, and we explored the impact of plant functional groups and soil chemistry on the soil microbial community. Except for Arbuscular Mycorrhizal fungi （AM fungi） biomarker 18：2ω6,9 increasing significantly, other biomarkers did not show a consistent trend with the elevational gradient. Microbial biomass quantified by total PLFAs did not show the elevational trend and had mean values ranging from 1.64 to 4.09 ktmol per g organic carbon （OC）, which had the maximum value at the highest site. Bacterial PLFAs exhibited a similar trend with total PLFAs, and its mean values ranged from 0.82 to 1.81 μmol （g OC）-1. The bacterial to fungal biomass ratios had the minimum value at the highest site, which might be related to temperature and soil total nitrogen （TN）. The ratios of Gram-negative to Gram-positive bacteria had a significantly negative correlation with soil TN and had the maximum value at the highest site. Leguminous plant coverage and soil TN explained 58% of the total variation in the soil microbial community and could achieve the same interpretation as the whole model. Other factors may influence the soil microbial community through interaction with leguminous plant coverage and soil TN. Soil chemistry and plant functional group composition in substantial amounts explained different parts of the variation within the soil microbial community, and the interaction between them had no impact on the soil microbial community maybe beeause long-term grazing greatly reduces litter. In sum, although there were obvious differences in soil microbial communities along the elevation gradient, there were no clear elevational trends found in general. Plant functional groups and soil chemistry respectively affect the different aspects of soil microbial community. Leguminous plant coverage and soil TN had important effects in shaping soil microbial community.

N-P fertilization did not reduce AMF abundance or diversity but alter AMF composition in an alpine grassland infested by a root hemiparasitic plant

Fertilization has been shown to have suppressive effects on arbuscular mycorrhizal fungi(AMF) and root hemiparasites separately in numerous investigations, but its effects on AMF in the presence of root hemiparasites remain untested. In view of the contrasting nutritional effects of AMF and root hemiparasites on host plants, we tested the hypothesis that fertilization may not show strong suppressive effects on AMF when a plant community was infested by abundant hemiparasitic plants. Plants and soil samples were collected from experimental field plots in Bayanbulak Grassland, where N and P fertilizers had been applied for three continuous years for control against a spreading root hemiparasite, Pedicularis kansuensis. Shoot and root biomass of each plant functional group were determined. Root AMF colonization levels, soil spore abundance, and extraradical hyphae length density were measured for three soil depths(0 e10 cm, 10 e20 cm, 20 e30 cm). Partial 18 S r RNA gene sequencing was used to detect AMF diversity and community composition. In addition, we analyzed the relationship between relative abundance of different AMF genera and environmental factors using Spearman's correlation method. In contrast to suppressive effects reported by many previous studies, fertilization showed no significant effects on AMF root colonization or AMF species diversity in the soil. Instead, a marked increase in soil spore abundance and extraradical hyphae length density were observed. However, fertilization altered relative abundance and AMF composition in the soil. Our results support the hypothesis that fertilization does not significantly influence the abundance and diversity of AMF in a plant community infested by P. kansuensis.

A remote sensing monitoring method for alpine grasslands desertification in the eastern Qinghai–Tibetan Plateau

Alpine grassland is the typical vegetation in the eastern Qinghai–Tibetan Plateau,which has important ecological service functions,and also supports the development of alpine stock farming.In recent years,under both the natural and human disturbance,alpine grasslands in this area have appeared to different degrees of desertification.A diagnosis of the desertification degree serves as the basis for grassland ecological restoration.This study constructs a comprehensive index based on remote sensing called alpine grassland desertification index(AGDI)to monitor the areas and degree of desertification.The most relevant indicators of desertification,namely,vegetation fraction,aboveground biomass,soil moisture,and land surface temperature,were selected to establish AGDI.The geographical detector is used to reselect and assess these indicators.The results show that the overall verification accuracy of AGDI is 82.05%.In particular,the accuracy of identifying severe desertification is the highest.Our study confirms that the desertification of alpine grasslands in the eastern Qinghai–Tibetan Plateau is characterized by fragmentation.Thus,Landsat-8 OLI data with a spatial resolution of 30 m is more suitable than MODIS data for alpine grasslands desertification monitoring.The research results can provide a methodological reference for monitoring desertification of alpine grasslands and other grassland regions in the world.

Alpine grassland fPAR change over the Northern Tibetan Plateau from 2002 to 2011

In this study, two different methods including Digital Camera and Reference Panel （DCRP） and traditional in situ fPAR observation for measuring the in situ point fPAR of very short alpine grass vegetation were compared, and the Moderate Resolution Imaging Spectroradiometer （MODIS） fPAR products were evaluated and validated by in situ point data on the alpine grassland over the Northern Tibetan Plateau, which is sensitive to climate change and vulnerable to anthropogenic activities. Results showed that the MODIS alpine grassland fPAR product, examined by using DCRP, and traditional in situ fPAR observation had a significant relationship at the spatial and temporal scales. The decadal MODIS fPAR trend analysis showed that, average growing season fPAR increased by 1.2 × 10^-4 per year and in total increased 0.86% from 2002 to 2011 in alpine grassland, when most of the fPAR increments occurred in southeast and center of the Northern Tibetan Plateau, the alpine grassland tended to recover from degradation slightly. However, climatic factors have influenced the various alpine grassland vegetation fPAR over a period of 10 years; precipitation significantly affected the alpine meadow fPAR in the eastern region, whereas temperature considerably influenced the alpine desert steppe fPAR in the west region. These findings suggest that the regional heterogeneity in alpine grassland fPAR results from various environmental factors, except for vegetation characteristics, such as canopy structure and leaf area.

Spatial-temporal dynamics of alpine grassland coverage and its response to climate warming in Mt.Qomolangma Nature Preserve during 2000-2019

The Qinghai-Tibet Plateau(QTP)has the largest and highest alpine grassland ecosystem in the world,which is considered to be the most sensitive and vulnerable ecosystem to climate change.Its dynamic changes and driving mechanism have always been widely researched.The Qomolangma National Nature Preserve(QNNP),with the largest altitude difference in the world,was selected as the study area to analyse the spatial-temporal dynamics of grassland coverage and the different characteristics of elevation gradients at the southern slope(SS)and northern slope(NS)with MODIS MOD13Q1 NDVI and MOD11A2 land surface temperature data from 2000to 2019 using the Mann-Kendall trend test and Theil-Sen slope methods.Further,the response mechanism of grassland coverage to climate warming is discussed.The results revealed that from 2000 to 2019,the grassland coverage change in the study area is mainly stable.The increased area proportion of grassland coverage on the southern slope is significantly higher than that on the northern slope,and the decreased area proportion of grassland coverage on the northern slope is significantly greater than that on the southern slope.The change characteristics of grassland coverage in the QNNP exhibit an obvious elevation gradient;the higher the elevation,the greater the increased area proportion of grassland coverage,particularly on the SS.The land surface temperature can be used as a proxy for analysing the temporal and spatial variation trends of air temperature in the QNNP.With the increase of the altitude,the land surface temperature rise rate on both the southern slope and northern slope exhibited an increasing trend,and the sensitivity of grassland coverage to temperature rise was higher on the northern slope.The water condition was the decisive factor for the horizontal and vertical spatial heterogeneity of the dynamic change of grassland coverage,and the melting of glaciers and thawing of permafrost were important sources of water for grassland growth in the QNNP.Climate warming promotes the growth of grassland in areas with a sufficient water supply,but adversely affects the growth of grassland in areas with insufficient water supplies,which will be further intensified by human activities.

Diversity of Plant Species of Alpine Grassland in Nakchu of Tibet, China

Characteristics of plant species diversity of two types of grassland communities (alpine meadow and alpine grassland) was examined and the relationship between species diversity and community dynamics was determined using GPS positioning and in situscrutinization of community of alpine grassland in Nakchu prefecture of Tibet Autonomous Region. The result indicated that: ① there was an unobvious difference between grassland communities in terms of richness index of plant, evenness index, dominant index and diversity index. The species diversity index followed the order Kobresia humilis meadow >Stipa purpurea steppe>K. littledalei meadow; ② the original community created high diversity, while the degenerative community suffering from serious disturbance was of low diversity; ③ the diversity of community plants was closely related to changes of species biomass, and the growth and decline of species in the community; ④ grazing disturbance is a key factor of community dynamics, leading to coexistence of various secondary successions of communities, diversified suitable habitats and species diversity. improvements

Effects of freezing intensity on soil solution nitrogen and microbial biomass nitrogen in an alpine grassland ecosystem on the Tibetan Plateau,China

The change of freeze-thaw pattern of the Tibetan Plateau under climate warming is bound to have a profound impact on the soil process of alpine grassland ecosystem;however,the research on the impact of the freeze-thaw action on nitrogen processes of the alpine grassland ecosystem on the Tibetan Plateau has not yet attracted much attention.In this study,the impact of the freezing strength on the soil nitrogen components of alpine grassland on the Tibetan Plateau was studied through laboratory freeze-thaw simulation experiments.The 0–10 cm topsoil was collected from the alpine marsh meadow and alpine meadow in the permafrost region of Beilu River.In the experiment,the soil samples were cultivated at –10℃,–7℃,–5℃,–3℃ and –1℃,respectively for three days and then thawed at 2℃ for one day.The results showed that after the freeze-thaw process,the soil microbial biomass nitrogen significantly decreased while the dissolved organic nitrogen and inorganic nitrogen significantly increased.When the freezing temperature was below –7℃,there was no significant difference between the content of nitrogen components,which implied a change of each nitrogen component might have a response threshold toward the freezing temperature.As the freeze-thaw process can lead to the risk of nitrogen loss in the alpine grassland ecosystem,more attention should be paid to the response of the soil nitrogen cycle of alpine grasslands on the Tibetan Plateau to the freeze-thaw process.

CO_2 processes in an alpine grassland ecosystem on the Tibetan Plateau

In this paper, the CO2 concentrations profile from 1.5 m depth in soil to 32 m height in atmosphere were measured from July 2000 to July 2001 in an alpine grassland ecosystem located in the permafrost area on the Tibetan Plateau, which revealed that CO2 concentrations varied greatly during this study period. Mean concentrations during the whole experiment in the atmosphere were absolutely lower than the CO2 concentrations in soil, which resulted in CO2 emissions from the alpine steppe soil to the atmosphere. The highest CO2 concentration was found at a depth of 1.5 m in soil while the lowest CO2 concentration occurred in the atmosphere. Mean CO2 concentrations in soil generally increased with depth. This was the compositive influence of the increasing soil moistures and decreasing soil pH, which induced the increasing biological activities with depth. Temporally, the CO2 concentrations at different layers in air remained a more steady state because of the atmospheric turbulent milking. During the seasonal variations, CO2 concentrations at surface soil interface showed symmetrical patterns, with the lowest accumulation of CO2 occurring in the late winter and the highest CO2 concentration in the growing seasons.

Research on the Soil Carbon Storage of Alpine Grassland under Different Land Uses in Qinghai-Tibet Plateau

In this article, we mainly analysis the soil carbon storage of the alpine grassland under different land uses in Qinghai-Tibet Plateau. The samples of this investigation include six experimental fields which are fenced mowing grassland, artificial grassland, winter and spring grazing meadowland, summer and autumn mild grazing land, summer and autumn moderate grazing pasture and summer and autumn severe grazing land and seven soil layers included 0 cm-5 cm, 5 cm-10 cm, 10 cm-20 cm, 20 cm-30 cm, 30 cm-50 cm, 50 cm-70 cm and 70 cm-100 cm. The results show that the soil carbon storage in different soil layers will gradually reduce and the difference was remarkable （P 〈 0.05）. What is more, the soil carbon storage of alpine grassland under different land uses has following sequence： winter and spring grazing grassland 〉 summer and autumn mild grazing land 〉 artificial grassland 〉 summer and autumn moderate grazing meadowland 〉 summer and autumn severe grazing pasture 〉 fenced mowing meadow, and the significant difference between them is remarkable （P 〈 0.05）.

Coarse fragment content influences estimates of soil C and N stocks of alpine grassland on the northeastern edge of Qinghai-Tibetan Plateau,China

Soil organic carbon(SOC)and total nitrogen(TN)stocks are usually calculated with samples collected using core samplers.Although the calculation considers the effects of gravel in soil samples,other coarse fragments such as stones or boulders in soil may not be collected due to the restricted diameter of core samplers.This would cause an incorrect estimation of soil bulk density and ultimately SOC and TN stocks.In this study,we compared the relative volume of coarse fragment and bulk density of fine earth determined by large size soil sampler with three core samplers.We also investigated the uncertainties in estimation of SOC and TN stocks caused by this soil sampler procedure in three typical alpine grasslands on the northeast edge of the Qinghai-Tibetan Plateau(QTP),China.Results show that(1)the relative volume and size of coarse fragment collected by large size sampler were significantly(p<0.05)higher and larger than those of core samplers,while bulk density of fine earth,SOC and TN stocks show opposite patterns in all grassland types;(2)SOC and TN stocks determined by core samplers were 17%-45%and 18%-46%higher than larger size sampler for three typical alpine grasslands;and(3)bulk density of fine earth,SOC and TN stocks exponentially decreased with the increasing of coarse fragment content.We concluded that core sampler methods significantly underestimated the volume occupied by coarse fragment but overestimated SOC and TN stocks.Thus,corrections should be made to the results from core samplers using large size samplers on regions with gravel and stone-rich soils in future studies.

Drought decreases the positive impact of warming on an alpine grassland community

Temperature and precipitation are the main factors determining plant community succession and aboveground net primary productivity(ANPP)in natural grasslands.However,most climate manipulative experiments have mainly focused on their impacts in isolation,especially in alpine regions.Here we explored the relative effects and interaction of warming and precipitation alteration on succession and ANPP using a 7-year experiment involving warming with precipitation alteration(increase(IP)or decrease(DP))in precipitation relative to ambient precipitation(AP)on the Tibetan Plateau.Our results showed that warming and warm-wet conditions increased species richness,diversity index,height and cover of overall species.Conversely,decreasing precipitation reduced them,but increased S.purpurea and the rate of change in community composition.Importantly,warming mitigated the impacts of decreased precipitation on plant community composition,and the interactive effects of warming and altered precipitation on cover,height and plant ANPP varied with year and plant species.Generally,warming increased community ANPP through increases in forb ANPP or non-dominant species ANPP and biodiversity regardless of change in precipitation.However,decreased precipitation reduced community ANPP via decreases in the ANPP of sedges and forbs and biodiversity.Precipitation alteration affected the relationship between biodiversity and community ANPP regardless of warming(IP<AP<DP).Therefore,generally warming and decreased precipitation have opposite effects on ANPP in the alpine grassland,suggesting that warming mitigated the negative impacts of drought on the ANPP of the alpine grassland.

Mechanism of plant-soil feedback in a degraded alpine grassland on the Tibetan Plateau

Although biotic and abiotic factors have been confirmed to be critical factors that affect community dynamics,their interactive effects have yet to be fully considered in grassland degradation.Herein,we tested how soil nutrients and microbes regulated plant-soil feedback(PSF)in a degraded alpine grassland.Our results indicated that soil total carbon(STC;from 17.66 to 12.55 g/kg)and total nitrogen(STN;from 3.16 to 2.74 g/kg)exhibited significant(P<0.05)decrease from non-degraded(ND)to severely degraded(SD).Despite higher nutrients in ND soil generating significantly(P<0.05)positive PSF(0.52)on monocots growth when the soil was sterilized,a high proportion of pathogens(36%)in ND non-sterilized soil resulted in a strong negative PSF on monocots.In contrast,the higher phenotypic plasticity of dicots coupled with a higher abundance of mutualists and saprophytes(70%)strongly promoted their survival and growth in SD with infertile soil.Our findings identified a novel mechanism that there was a functional group shift from monocots with higher vulnerability to soil pathogens in the ND fertile soil to dicots with higher dependence on nutritional mutualists in the degraded infertile soil.The emerging irreversible eco-evolutionary in PSF after degradation might cause a predicament for the restoration of degraded grassland.

Development of long-term spatiotemporal continuous NDVI products for alpine grassland from 1982 to 2020 in the Qinghai–Tibet Plateau,China

Background:The time-series data of the Normalized Difference Vegetation Index(NDVI)is a crucial indicator for global and regional vegetation monitoring.However,the current assessment of global and regional long-term vegetation changes is subject to large uncertainties due to the lack of spatiotemporally continuous time-series data sets.Methods:In this study,a long time-series monthly NDVI data set with a spatial resolution of 250m from 1982 to 2020 was developed by combining Moderate Resolution Imaging Spectroradiometer(MODIS)and AVHRR(Advanced Very High-Resolution Radiometer)time-series NDVI products using the Random Forest(RF)downscaling model.Results:Compared to the MODIS NDVI product,the fused product shows RMSE and mean absolute error ranging from 0 to 0.075 and from 0 to 0.05,respectively,with R^(2)values mostly above 0.7.Conclusions:The long time-series NDVI products generated in this study are reliable in terms of accuracy and have great potential for long-term dynamic monitoring of terrestrial ecosystems on the Qinghai–Tibet Plateau.

Differences in respiration components and their dominant regulating factors across three alpine grasslands on the Qinghai−Tibet Plateau

Greenhouse gases(GHGs)emissions from high-cold terrestrial ecosystems underlain by permafrost on the Qinghai–Tibet Plateau(QTP)have received widespread attention.However,the dominant factors regulating ecosystem respiration(Re)and its components(soil respiration Rs and heterotrophic respiration Rh)and how the differences in carbon emissions from different ecotypes and seasons remain are still unclear.We conducted a 2-year field investigation(August 2018 to October 2020)and applied the structural equation model(SEM)to clarify the changes in the factors controlling the respiration components during different seasons.The results indicate that the R_(e)and its controlling factors in three alpine grassland ecosystems(alpine steppe,alpine meadow,and swamp meadow)vary with seasons.Furthermore,autotrophic respiration(Ra)contributes the most to the seasonal changes in R_(e).The R_(e)gradually increases in the early growing season and rapidly decreases in the late growing season.Rh remains relatively stable during the year.Under these seasonal variations in the respiration components,the dominant factors controlling R_(e)in the nongrowing season are the temperature of the atmosphere–soil interface(heat flux,atmospheric temperature,and soil temperature at 5 cm depth)and microbial activity(microbial carbon and pH)with the variable importance projections>1.5.During the growing season,the dominant factors regulating R_(e),Rs,and Rh are the soil temperature with a standardized direct effect(SDE)of 0.424,soil nutrient conditions(total nitrogen and pH)with SDEs of 0.570–0.614,and microbial activity(microbial carbon)with a SDE of 0.591,respectively.In addition,meteorological conditions have an important impact on the respiration components during the growing season.Specifically,the atmospheric vapor pressure is the dominant factor regulating the three respiration components(standardized total effects=0.44−0.53,p<0.001).The optimal soil water contents during the growing season(water content at which R_(e)reaches the maximum)are 10%in the alpine steppe,13%–15%in the alpine meadow,and 40%–43%in the swamp meadow,respectively.The effect of the soil water content on R_(e)is more important in arid ecosystems(alpine steppe and alpine meadow)than in wet ecosystem(swamp meadow).The alleviation of water limitations in arid ecosystems may potentially increase R_(e).

Vegetation characteristics and soil properties of artificially remediated grasslands:The case study of the Shimenhe mining area in Qilian Mountains,northwest China

The mining of limestone mines plays a crucial role in societal and economic advancement.However,mining activities have led to destructive variations in grassland ecology and soil,causing numerous environmental problems,and effective artificial restoration measures have been used to restore grasslands in the Shimenhe mining areas to different degrees.In this study,we investigated,examined and analyzed plant community structure and its correlation with soil properties across varying degrees of alpine grassland restoration in Qilian Mountains Shimenhe restoration mines using the sample method,and studied the changes in species diversity using five diversity indexes(Simpson index,Shannon index,Margalef index,Dominance index and Evenness index).This study showed that the plant community characteristics with high recovered degree(HRD)>middle recovered degree(MRD)>low recovered degree(LRD)>very low recovered degree(VLRD),11 plant genera comprising 11 species across 10 families were identified.Dominant families with robust ecological adaptability included Leguminosae,Rosaceae,Gramineae,Asteraceae,and Salicaceae.The highest Simpson,Shannon,Margalef and Evenness index of HRD grassland community species were 0.82,1.96,1.66 and 0.89,respectively.The highest Dominance index of VLRD grassland community species was 0.34,which required several restoration methods such as spraying and mulching.Soil pH and EC tended to decrease with increasing restoration,SOC,SMC,TP,AP,NH4-N,TN,AN and NO3-N tended to increase and the content of soil environmental factors contributed to vegetation growth across various restoration levels the mine grassland.In conclusion,our study indicated that the community structure gradually diversified and soil properties changed positively with the increase of restoration degrees in the Qilian Mountains Shimenhe mine,and the best results of HRD restoration were obtained.This study provides the theoretical basis for the restoration and conservation of grasslands in mining areas by demonstrating examined the correlation between plant characteristics and soil properties in restored grasslands in alpine mining areas.

Impact of Drought Stress on Net CO_2 Exchange above an Alpine Grassland Ecosystem in the Central Tibetan Plateau

Drought may impact the net ecosystem exchange of CO2 （NEE） between grassland ecosystems and the atmosphere during growth seasons. Here, carbon dioxide exchange and controlling factors in alpine grassland under drought stress in the hinterland of Tibetan Plateau （Damxung, Tibet, China） were investigated. Data were obtained using the covariance eddy technique in 2009. Severe drought stress appeared in the early growing season （May to early July） and September. Drought conditions during the early growing season limited grass production and the green leaf area index （GLAD increased slowly, with an obvious decline in June. When encountering severe water stress, diurnal patterns of NEE in the growth season altered with a peak carbon release around 16：00 h or a second carbon uptake period before sunset. NEE variations in daytime related most closely with O other than PAR when daily averaged @〈0.1 m3 m 3. Seasonal patterns of gross primary production （GPP） and NEE were also influenced by drought： the maximum and minimum of daily-integrated NEE were 0.9 g C m-2 d-1 on 3 July 2009, and -1.3 g C m-2 d-1 on 12 August 2009 with a GPP peak （-2.3 g C m-2 d-1） on the same day, respectively. Monthly NEE from May to July remained as carbon release and increased gradually; peak values of monthly NEE and GPP both appeared in August, but that of ecosystem respiration （R^co） was reached in July. Annual NEE, GPP and Reco of the alpine grassland ecosystem were 52.4, -158.1 and 210.5 g C m-2, respectively. Therefore, the grassland was a moderate source of COs to the atmosphere in this dry year. Interannual variation in NEE was likely related to different water conditions in the growing season. The three greatest contributors to seasonal variation in NEE, GPP and R^co respectively were GLAI〉Ta〉O, GLAI〉O〉PPT, and Ta〉GLAI〉PAR. Seasonality of GLAI explained 60.7% and 76.1% of seasonal variation in NEE and GPP, respectively. GPP or NEE was more sensitive than Reco to variation in GLAI, and ecosystem water conditions.