[Objectives] To clarify the appropriate harvest period of Alpinia oxyphylla Miq. seed and improve the quality of seeds. [Methods] In this experiment,the basic parameters and germination characteristics of A. oxyphylla...[Objectives] To clarify the appropriate harvest period of Alpinia oxyphylla Miq. seed and improve the quality of seeds. [Methods] In this experiment,the basic parameters and germination characteristics of A. oxyphylla Miq. seeds during different harvest periods were analyzed and compared. [Results] The results showed that the A. oxyphylla Miq. fruit picking period was closely related to the seed quality,and the seed maturity was highest 100 d after the flowering of A. oxyphylla Miq.,that is,the A. oxyphylla Miq. fruit skin turned from green to yellow( yellow-green),the peel had a small number of brown spots,it was spicy enough,the fruit dry/fresh weight ratio was greater than0. 34,the seed thousand kernel weight reached 11 g or more,and the dried seed moisture content was 13. 01%. Using the TTC method,the seed viability index was determined to be 85%,and in this case,it was the best time of harvesting seed. The water permeability of A. oxyphylla Miq. seed was poor,and the seed imbibition and water absorption time was long,about 72 h. The maximum water absorption rate was about23. 71% at the imbibition and water absorption stage,and it was inferred that the A. oxyphylla Miq. seeds were hard seeds. The germination time of A. oxyphylla Miq. seeds was long,the mature A. oxyphylla Miq. seeds began to germinate on the 12 th d,and the germination rate reached 75. 56% on the 50 th d. [Conclusions] This study determined the optimum harvest period of A. oxyphylla Miq. seeds,clarified the biological characteristics of slow seed water absorption and long germination cycle,and concluded that the seed coat was the main factor leading to slow seed water absorption and long seed germination cycle.展开更多
[目的]观察益智仁水提取物(A lp in ia oxyphylla M iq.fru it,AOF)对脑老化小鼠海马SOD活力及海马蛋白含量的影响.[方法]选用健康成年昆明种小鼠60只,雌雄各半,随机数字表法将其分成5组:对照组、模型组、脑复康组、AOF低剂量组、AOF高...[目的]观察益智仁水提取物(A lp in ia oxyphylla M iq.fru it,AOF)对脑老化小鼠海马SOD活力及海马蛋白含量的影响.[方法]选用健康成年昆明种小鼠60只,雌雄各半,随机数字表法将其分成5组:对照组、模型组、脑复康组、AOF低剂量组、AOF高剂量组.用D-半乳糖皮下注射诱导小鼠脑老化模型,采用学习记忆行为训练和生化测定结合的方法,观察AOF对小鼠学习记忆能力的影响,测定海马组织超氧化物歧化酶(SOD)和海马蛋白含量.[结果]①AOF对脑老化小鼠学习记忆能力的影响:模型组达标所需训练次数显著高于对照组(p<0.01),AOF低、高剂量组达标所需训练次数显著低于模型组(p<0.01).②AOF对脑老化小鼠海马SOD活力的影响:模型组小鼠海马SOD活力显著低于对照组(p<0.05),AOF低、高剂量组小鼠海马SOD活力显著高于模型组(p<0.05).③AOF对脑老化小鼠海马蛋白含量的影响:与对照组比,模型组小鼠海马蛋白含量显著降低(p<0.01);与模型组相比,AOF低、高剂量组小鼠海马蛋白含量显著增高(p<0.01).[结论]AOF可提高脑老化小鼠海马SOD活力,增加海马蛋白含量,对D-半乳糖诱导脑老化小鼠的学习记忆障碍具有显著的改善作用.展开更多
基金Supported by the Fundamental Research Funds for Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences(16300420170291630042017008)
文摘[Objectives] To clarify the appropriate harvest period of Alpinia oxyphylla Miq. seed and improve the quality of seeds. [Methods] In this experiment,the basic parameters and germination characteristics of A. oxyphylla Miq. seeds during different harvest periods were analyzed and compared. [Results] The results showed that the A. oxyphylla Miq. fruit picking period was closely related to the seed quality,and the seed maturity was highest 100 d after the flowering of A. oxyphylla Miq.,that is,the A. oxyphylla Miq. fruit skin turned from green to yellow( yellow-green),the peel had a small number of brown spots,it was spicy enough,the fruit dry/fresh weight ratio was greater than0. 34,the seed thousand kernel weight reached 11 g or more,and the dried seed moisture content was 13. 01%. Using the TTC method,the seed viability index was determined to be 85%,and in this case,it was the best time of harvesting seed. The water permeability of A. oxyphylla Miq. seed was poor,and the seed imbibition and water absorption time was long,about 72 h. The maximum water absorption rate was about23. 71% at the imbibition and water absorption stage,and it was inferred that the A. oxyphylla Miq. seeds were hard seeds. The germination time of A. oxyphylla Miq. seeds was long,the mature A. oxyphylla Miq. seeds began to germinate on the 12 th d,and the germination rate reached 75. 56% on the 50 th d. [Conclusions] This study determined the optimum harvest period of A. oxyphylla Miq. seeds,clarified the biological characteristics of slow seed water absorption and long germination cycle,and concluded that the seed coat was the main factor leading to slow seed water absorption and long seed germination cycle.
文摘[目的]观察益智仁水提取物(A lp in ia oxyphylla M iq.fru it,AOF)对脑老化小鼠海马SOD活力及海马蛋白含量的影响.[方法]选用健康成年昆明种小鼠60只,雌雄各半,随机数字表法将其分成5组:对照组、模型组、脑复康组、AOF低剂量组、AOF高剂量组.用D-半乳糖皮下注射诱导小鼠脑老化模型,采用学习记忆行为训练和生化测定结合的方法,观察AOF对小鼠学习记忆能力的影响,测定海马组织超氧化物歧化酶(SOD)和海马蛋白含量.[结果]①AOF对脑老化小鼠学习记忆能力的影响:模型组达标所需训练次数显著高于对照组(p<0.01),AOF低、高剂量组达标所需训练次数显著低于模型组(p<0.01).②AOF对脑老化小鼠海马SOD活力的影响:模型组小鼠海马SOD活力显著低于对照组(p<0.05),AOF低、高剂量组小鼠海马SOD活力显著高于模型组(p<0.05).③AOF对脑老化小鼠海马蛋白含量的影响:与对照组比,模型组小鼠海马蛋白含量显著降低(p<0.01);与模型组相比,AOF低、高剂量组小鼠海马蛋白含量显著增高(p<0.01).[结论]AOF可提高脑老化小鼠海马SOD活力,增加海马蛋白含量,对D-半乳糖诱导脑老化小鼠的学习记忆障碍具有显著的改善作用.
