Establishment of allergic rhinitis model in mice induced by modified OVA and aluminum adjuvant

Objective:In order to further study allergic rhinitis,the feasibility of the improved method of establishing mouse allergic rhinitis model by OVA combined with aluminum adjuvant was explored.Methods:16 BALB/c mice(SPF grade)were randomly divided into the control group and the OVA model group.In the OVA model group,mice were given intraperitoneal injection of OVA solution for sensitization(100μg/ml)at weeks 1,2,and 3,and each mouse was injected intraperitoneally with 0.2 ml.On the 22nd to 28th days,the mice were given an OVA solution(100 mg/ml)for nasal stimulation,10μl per mouse,and 5μl per nostril for 7 consecutive days.The general behavior,body weight,and survival rate of the mice were observed on days 22-28,respectively.After 15 minutes of challenge on days 25 and 28,the number of sneezing and sniffing within 15 minutes was recorded.On day 29,spleen and thymus indexes of mice were evaluated,and blood was collected from the orbit to isolate serum,the IgE level was measured,and the histological changes of the nasal mucosa of the mice were observed.Results:OVA can cause significant weight loss in mice,and the weight of mice in the OVA model group is significantly lower than that in the control group(P<0.05).On the 25th and 28th days,the number of sneezes in the OVA model group was significantly higher than that in the control group[(19.27±4.89)vs(4.45±1.25);(19.78±4.92)vs(4.52±1.28);P<0.05].The number of nasal scratching in the OVA model group was significantly higher than that in the control group[(23.14±7.17)vs(13.52±7.64);(23.75±7.35)vs(13.60±7.71);P<0.05].The spleen index of the OVA model group was significantly higher than that of the control group[(8.64±1.49)mg/g vs(4.04±0.65)mg/g,P<0.05],and the thymus index of the OVA model group was significantly lower than that of the control group[(0.75±0.54)mg/g vs(1.87±0.65)mg/g,P<0.05].The IgE level of the OVA model group were significantly higher than that of the control group[(932.86±352.34)μg/ml vs(99.82±0.97)μg/ml,P<0.05].The nasal mucosal tissue lesions in the model group were obvious.Conclusion:In this experiment,a modified OVA-aluminum adjuvant was used to successfully establish the mice model of allergic rhinitis.