SYNERGISM BETWEEN PHOTODYNAMIC THERAPY OF ALUMINUM SULFONATED PHTHALOCYANINE AND HYPERTHERMIA ON MOUSE HEPATOMA IN VIVO AND IN VITRO

Mouse hepatoma ascites was inoculated sc into the footpad of right hindleg. On d 7 after inoculation the tunior site was treated by heat (44 ℃, 25 min), photodynamic therapy (PDT) of aluminum sulfonated phthalocyanine (AlSPc), and PDT plus heat. The hepatoma growth was almost completely inhibited and a high percentage of cure obtained in the combination group.The succinic dehydrogenase (SD), superoxide dismutase (SOD) and glutathione (GSH) of hepatonia cells were not affected by heat alone, but when heat was used following PDT, the SD, SOD and GSH in hepatonia calls were significantly inhibited.

磺化铝酞菁光动力治疗合并热疗对小鼠移植肝癌的协同作用

昆明小鼠右后脚垫或右大腿外侧皮下接种肝癌腹水，随机分组。光动力治疗（PDT）组，接种瘤细胞后第6无尾静脉注入磺化铝酞菁（ALSPc）10～20mg／kg，24小时后肿瘤部位照红光。热疗组，大腿部肿瘤用微波热疗（42．5℃、20分钟）或脚垫部肿瘤浸入恒温水浴内（44℃、25分钟）。合并治疗组，先行PDT治疗，10分钟后进行热疗。单独热疗或单独PDT治疗，小鼠肿瘤受到明显抑制，4O天内有50％左右肿瘤消退。合并治疗组对肿瘤的抑制更为显著，第20天和第40天的肿痛消退率分别比单纯热疗组和单纯PDT组高，统计上差异显著。