Type 2 diabetes mellitus(T2DM)exacerbates irreversible bone loss in periodontitis,but the mechanism of impaired bone regeneration caused by the abnormal metabolic process of T2DM remains unclear.Exosomes are regarded ...Type 2 diabetes mellitus(T2DM)exacerbates irreversible bone loss in periodontitis,but the mechanism of impaired bone regeneration caused by the abnormal metabolic process of T2DM remains unclear.Exosomes are regarded as the critical mediator in diabetic impairment of regeneration via organ or tissue communication.Here,we find that abnormally elevated exosomes derived from metabolically impaired liver in T2DM are significantly enriched in the periodontal region and induced pyroptosis of periodontal ligament cells(PDLCs).Mechanistically,fatty acid synthase(Fasn),the main differentially expressed molecule in diabetic exosomes results in ectopic fatty acid synthesis in PDLCs and activates the cleavage of gasdermin D.Depletion of liver Fasn effectively mitigates pyroptosis of PDLCs and alleviates bone loss.Our findings elucidate the mechanism of exacerbated bone loss in diabetic periodontitis and reveal the exosome-mediated organ communication in the“liver-bone”axis,which shed light on the prevention and treatment of diabetic bone disorders in the future.展开更多
<strong>Background:</strong> Diabetes increases periodontal disease risk and its complications, which are worsened with age or faulty restorations. <strong>Aim:</strong> Evaluate periodontal he...<strong>Background:</strong> Diabetes increases periodontal disease risk and its complications, which are worsened with age or faulty restorations. <strong>Aim:</strong> Evaluate periodontal health among patients with diabetes treated with different dental restorations. <strong>Materials and Methods:</strong> This comparative cross-sectional study was conducted at the faculty of dentistry, Najran University, KSA, from March 2018 to February 2020. It included 260 diabetics, treated with different dental restorations, divided into four groups, each comprising 65 patients aged 45 - 60 years: Group 1 (G1), no restorations (control group);Group 2 (G2), Class II amalgam restorations;Group 3 (G3), Class II composite resin restorations;and Group 4 (G4), three-unit posterior metal ceramic prostheses. The Ages of all restorations ranged from 8 to 10 years. All groups were assessed for the Plaque Index (PI) and Gingival Index (GI), Overhangs Rate (OR), Alveolar Bone Loss (ABL) (the latter two using panoramic X-ray). HbA1c and duration of participants were also assessed and compared. <strong>Results:</strong> The mean HbA1c results of the groups ranged from 8.1% to 9.5%, and their mean diabetes durations ranged from 7.2 to 12 years. All the groups showed worse periodontal scores, ranging from 2.45 - 2.95 for PI and 2.25 - 2.8 for GI. G2 had higher scores than G1 and G3 (<em>p</em> < 0.001). G4 had the highest two recorded scores (<em>p</em> < 0.001). G2 had a higher rate of overhangs than G3 (<em>p</em> < 0.037). The mean ABL was 44% (SD 6.4) for G1, 56.0% (SD 6.7) for G2, 46.5% (SD 5.8) for G3, and 74.5% (SD 6.4) for G4. The lowest values were recorded in G1 and G3 (<em>p</em> = 0.987). The highest value was observed in G4, which was significantly higher than other groups (<em>p</em> < 0.001). <strong>Conclusion: </strong>The worse periodontal parameters among subjects treated with either three-unit metal ceramic or Class II amalgam restorations indicated severe periodontal destruction. Untreated subjects and subjects treated with Class II composite resin had relatively better periodontal status.展开更多
目的探讨2型糖尿病患者血清骨保护素(osteoprotegerin,OPG)水平与骨密度(bone mineral density,BMD)的关系.方法:选择2型糖尿病患者108例,用双能X线吸收法(DEXA)测定BMD,根据BMD将患者分为3组,骨量正常(A)组33例,骨量减少(B)...目的探讨2型糖尿病患者血清骨保护素(osteoprotegerin,OPG)水平与骨密度(bone mineral density,BMD)的关系.方法:选择2型糖尿病患者108例,用双能X线吸收法(DEXA)测定BMD,根据BMD将患者分为3组,骨量正常(A)组33例,骨量减少(B)组39例及骨质疏松(C)组36例,测定血清OPG水平,并进行各项生化指标的检测,和45例正常人做对照.结果:2型糖尿病组BMD低于对照组(P〈0.05);2型糖尿病组血清OPG水平明显高于对照组(P〈0.01);骨量减少组及骨质疏松组OPG浓度高于骨量正常组(P〈0.05).多元逐步回归分析表明,2型糖尿病患者血清骨保护素与BMD呈负相关(r=-0.168,P〈0.05).结论:2型糖尿病患者较正常人易发生骨质疏松.2型糖尿病患者血清OPG水平与BMD呈负相关,OPG为影响BMD的独立相关因素,OPG参与调节骨代谢过程,可能参与了糖尿病的发生和发展.OPG浓度的测定可能对2型糖尿病患者骨质疏松症的诊断有一定的帮助.展开更多
基金supported by the Major Program of the National Natural Science Foundation of China(81991500,81991502,81991504)Shaanxi provincial key research and development program(2023-ZDLSF-49)the National Postdoctoral Program for Innovative Talents of China(BX20220394).
文摘Type 2 diabetes mellitus(T2DM)exacerbates irreversible bone loss in periodontitis,but the mechanism of impaired bone regeneration caused by the abnormal metabolic process of T2DM remains unclear.Exosomes are regarded as the critical mediator in diabetic impairment of regeneration via organ or tissue communication.Here,we find that abnormally elevated exosomes derived from metabolically impaired liver in T2DM are significantly enriched in the periodontal region and induced pyroptosis of periodontal ligament cells(PDLCs).Mechanistically,fatty acid synthase(Fasn),the main differentially expressed molecule in diabetic exosomes results in ectopic fatty acid synthesis in PDLCs and activates the cleavage of gasdermin D.Depletion of liver Fasn effectively mitigates pyroptosis of PDLCs and alleviates bone loss.Our findings elucidate the mechanism of exacerbated bone loss in diabetic periodontitis and reveal the exosome-mediated organ communication in the“liver-bone”axis,which shed light on the prevention and treatment of diabetic bone disorders in the future.
文摘<strong>Background:</strong> Diabetes increases periodontal disease risk and its complications, which are worsened with age or faulty restorations. <strong>Aim:</strong> Evaluate periodontal health among patients with diabetes treated with different dental restorations. <strong>Materials and Methods:</strong> This comparative cross-sectional study was conducted at the faculty of dentistry, Najran University, KSA, from March 2018 to February 2020. It included 260 diabetics, treated with different dental restorations, divided into four groups, each comprising 65 patients aged 45 - 60 years: Group 1 (G1), no restorations (control group);Group 2 (G2), Class II amalgam restorations;Group 3 (G3), Class II composite resin restorations;and Group 4 (G4), three-unit posterior metal ceramic prostheses. The Ages of all restorations ranged from 8 to 10 years. All groups were assessed for the Plaque Index (PI) and Gingival Index (GI), Overhangs Rate (OR), Alveolar Bone Loss (ABL) (the latter two using panoramic X-ray). HbA1c and duration of participants were also assessed and compared. <strong>Results:</strong> The mean HbA1c results of the groups ranged from 8.1% to 9.5%, and their mean diabetes durations ranged from 7.2 to 12 years. All the groups showed worse periodontal scores, ranging from 2.45 - 2.95 for PI and 2.25 - 2.8 for GI. G2 had higher scores than G1 and G3 (<em>p</em> < 0.001). G4 had the highest two recorded scores (<em>p</em> < 0.001). G2 had a higher rate of overhangs than G3 (<em>p</em> < 0.037). The mean ABL was 44% (SD 6.4) for G1, 56.0% (SD 6.7) for G2, 46.5% (SD 5.8) for G3, and 74.5% (SD 6.4) for G4. The lowest values were recorded in G1 and G3 (<em>p</em> = 0.987). The highest value was observed in G4, which was significantly higher than other groups (<em>p</em> < 0.001). <strong>Conclusion: </strong>The worse periodontal parameters among subjects treated with either three-unit metal ceramic or Class II amalgam restorations indicated severe periodontal destruction. Untreated subjects and subjects treated with Class II composite resin had relatively better periodontal status.
文摘目的探讨2型糖尿病患者血清骨保护素(osteoprotegerin,OPG)水平与骨密度(bone mineral density,BMD)的关系.方法:选择2型糖尿病患者108例,用双能X线吸收法(DEXA)测定BMD,根据BMD将患者分为3组,骨量正常(A)组33例,骨量减少(B)组39例及骨质疏松(C)组36例,测定血清OPG水平,并进行各项生化指标的检测,和45例正常人做对照.结果:2型糖尿病组BMD低于对照组(P〈0.05);2型糖尿病组血清OPG水平明显高于对照组(P〈0.01);骨量减少组及骨质疏松组OPG浓度高于骨量正常组(P〈0.05).多元逐步回归分析表明,2型糖尿病患者血清骨保护素与BMD呈负相关(r=-0.168,P〈0.05).结论:2型糖尿病患者较正常人易发生骨质疏松.2型糖尿病患者血清OPG水平与BMD呈负相关,OPG为影响BMD的独立相关因素,OPG参与调节骨代谢过程,可能参与了糖尿病的发生和发展.OPG浓度的测定可能对2型糖尿病患者骨质疏松症的诊断有一定的帮助.