Currently, the most commonly used treatment methods for repairing alveolar furcation defects are periodontal guided tissue regeneration （GTR） and bone grafting. The objective of this study was to investigate the eff...Currently, the most commonly used treatment methods for repairing alveolar furcation defects are periodontal guided tissue regeneration （GTR） and bone grafting. The objective of this study was to investigate the effects of simvastatin/methylcellulose gel on bone regeneration in alveolar defects in miniature pigs. Methods Alveolar defects were produced in 32 teeth （the third and fourth premolars） of 4 miniature pigs. The 32 experimental teeth were divided into 5 groups comprising control （C） and treatment （T） teeth： （1） empty defects without gel （group CO, n=4）; （2） defects injected with methylcellulose gel （group C1, n=4）; （3） defects injected with 0.5 rag/50 pl simvastatin/methylcellulose gel （group T1, n=8）; （4） defects injected with 1.5 mg/50 μl simvastatin/methylcellulose gel （group T2, n=8）; and （5） defects injected with 2.2 mg/50 μl simvastatin/methylcellulose gel （group T3, n=8）. Every week after surgery, the furcation sites were injected once with gel. At the eighth week afte（ surgery, the 4 pigs were sacrificed and underwent macroscopic observation, descriptive histologic examination, and regenerate bone quantitative histologic examination. Results At 8 weeks after surgery, the defect sites in the treatment groups were completely filled in with new bone and fibrous tissue. There was little new bone in the CO and C1 groups, and only a small number of osteoblasts and proliferative vessels could be seen on microscopic examination. Conclusions Miniature pigs are an ideal experimental animal for establishing a model of alveolar defects using a surgical method. Local application of simvastatin/methylcellulose gel can stimulate the regeneration of alveolar bone in furcation defect sites, because it promotes the proliferation of osteoblasts. The best dose of simvastatin gel to stimulate bone regeneration is 0.5 mg.展开更多
Resorption and loss of alveolar bone leads to oral dysfunction and loss of natural or implant teeth. Biomimetic delivery of growth factors based on stem cell recruitment and osteogenic differentiation, as the key step...Resorption and loss of alveolar bone leads to oral dysfunction and loss of natural or implant teeth. Biomimetic delivery of growth factors based on stem cell recruitment and osteogenic differentiation, as the key steps in natural alveolar bone regenerative process, has been an area of intense research in recent years. A mesoporous self-healing hydrogel(DFH) with basic fibroblast growth factor(bFGF) entrapment and transforming growth factor β3(TGFβ3)-loaded chitosan microspheres(CMs) was developed. The formulation was optimized by multiple tests of self-healing, in-bottle inversion, SEM, rheological, swelling rate and in vitro degradation. In vitro tubule formation assays, cell migration assays, and osteogenic differentiation assays confirmed the ability of DFH to promote blood vessels, recruit stem cells, and promote osteogenic differentiation. The optimum DFH formula is 0.05 ml 4ArmPEG-DF(20%) added to 1 ml CsGlu(2%) containing bFGF(80 ng) and TGFβ3-microspheres(5 mg). The results of in vitro release studied by Elisa kit, indicated an 95% release of b FGF in7 d and long-term sustained release of TGFβ3. For alveolar defects rat models, the expression levels of CD29 and CD45, the bone volume fraction, trabecular number, and trabecular thickness of new bone monitored by Micro-CT in DFH treatment groups were significantly higher than others(*P < 0.05, vs Model). HE and Masson staining show the same results.In conclusion, DFH is a design of bionic alveolar remodelling microenvironment, that is in early time microvessels formed by b FGF provide nutritious to recruited endogenous stem cells, then TGFβ3 slowly released speed up the process of new bones formation to common facilitate rat alveolar defect repair. The DFH with higher regenerative efficiency dovetails nicely with great demand due to the requirement of complicated biological processes.展开更多
目的:探讨浓缩生长因子纤维蛋白膜联合Bio-Oss骨粉修复拔牙术后牙槽骨缺损的疗效。方法:选择2018年1月-2020年1月笔者医院收治的因拔除下颌阻生第三磨牙致第二磨牙远中骨缺损患者76例,予以随机数字表法分为对照组(Bio-Oss骨粉植入,38例...目的:探讨浓缩生长因子纤维蛋白膜联合Bio-Oss骨粉修复拔牙术后牙槽骨缺损的疗效。方法:选择2018年1月-2020年1月笔者医院收治的因拔除下颌阻生第三磨牙致第二磨牙远中骨缺损患者76例,予以随机数字表法分为对照组(Bio-Oss骨粉植入,38例)和观察组(CGF膜联合Bio-Oss骨粉植入,38例)。手术后3 d复诊观察患者术后疼痛、开口度情况,比较两组患者术后1周创面愈合情况以及术前、术后1个月和3个月牙槽骨密度、牙槽嵴高度及牙槽嵴宽度,记录术后不良反应。结果:对照组术后3 d VAS评分(3.35±0.47)分,观察组为(2.67±0.42)分,对照组开口度(2.75±0.46)cm,观察组(2.68±0.51)cm,两组比较差异均无统计学意义(P>0.05)。术后1周,观察组创面愈合情况好于对照组,差异有统计学意义(P<0.05);术后1个月、术后3个月,观察组牙槽骨密度、牙槽嵴高度、牙槽嵴宽度均高于对照组,差异有统计学意义(P<0.05)。对照组术后不良反应发生率23.68%,高于观察组的5.26%,差异有统计学意义(P<0.05)。结论:浓缩生长因子纤维蛋白膜联合Bio-Oss骨粉在第二磨牙远中骨缺损修复中发挥着重要作用,值得临床推广应用。展开更多
文摘Currently, the most commonly used treatment methods for repairing alveolar furcation defects are periodontal guided tissue regeneration （GTR） and bone grafting. The objective of this study was to investigate the effects of simvastatin/methylcellulose gel on bone regeneration in alveolar defects in miniature pigs. Methods Alveolar defects were produced in 32 teeth （the third and fourth premolars） of 4 miniature pigs. The 32 experimental teeth were divided into 5 groups comprising control （C） and treatment （T） teeth： （1） empty defects without gel （group CO, n=4）; （2） defects injected with methylcellulose gel （group C1, n=4）; （3） defects injected with 0.5 rag/50 pl simvastatin/methylcellulose gel （group T1, n=8）; （4） defects injected with 1.5 mg/50 μl simvastatin/methylcellulose gel （group T2, n=8）; and （5） defects injected with 2.2 mg/50 μl simvastatin/methylcellulose gel （group T3, n=8）. Every week after surgery, the furcation sites were injected once with gel. At the eighth week afte（ surgery, the 4 pigs were sacrificed and underwent macroscopic observation, descriptive histologic examination, and regenerate bone quantitative histologic examination. Results At 8 weeks after surgery, the defect sites in the treatment groups were completely filled in with new bone and fibrous tissue. There was little new bone in the CO and C1 groups, and only a small number of osteoblasts and proliferative vessels could be seen on microscopic examination. Conclusions Miniature pigs are an ideal experimental animal for establishing a model of alveolar defects using a surgical method. Local application of simvastatin/methylcellulose gel can stimulate the regeneration of alveolar bone in furcation defect sites, because it promotes the proliferation of osteoblasts. The best dose of simvastatin gel to stimulate bone regeneration is 0.5 mg.
基金supported by grants from the Guangzhou Science and Technology Program Key Project(Grant No.201803010044)Guangdong Province College Characteristic Innovation Project(2019KTSCX011)+2 种基金Guangdong Province Natural Sciences Fund Project(2021A1515012480)the Key Areas Research and Development Program of Guangzhou(202103030003)Guangdong Province Special Fund Projects(Yueziranzihe,2021,No.50).
文摘Resorption and loss of alveolar bone leads to oral dysfunction and loss of natural or implant teeth. Biomimetic delivery of growth factors based on stem cell recruitment and osteogenic differentiation, as the key steps in natural alveolar bone regenerative process, has been an area of intense research in recent years. A mesoporous self-healing hydrogel(DFH) with basic fibroblast growth factor(bFGF) entrapment and transforming growth factor β3(TGFβ3)-loaded chitosan microspheres(CMs) was developed. The formulation was optimized by multiple tests of self-healing, in-bottle inversion, SEM, rheological, swelling rate and in vitro degradation. In vitro tubule formation assays, cell migration assays, and osteogenic differentiation assays confirmed the ability of DFH to promote blood vessels, recruit stem cells, and promote osteogenic differentiation. The optimum DFH formula is 0.05 ml 4ArmPEG-DF(20%) added to 1 ml CsGlu(2%) containing bFGF(80 ng) and TGFβ3-microspheres(5 mg). The results of in vitro release studied by Elisa kit, indicated an 95% release of b FGF in7 d and long-term sustained release of TGFβ3. For alveolar defects rat models, the expression levels of CD29 and CD45, the bone volume fraction, trabecular number, and trabecular thickness of new bone monitored by Micro-CT in DFH treatment groups were significantly higher than others(*P < 0.05, vs Model). HE and Masson staining show the same results.In conclusion, DFH is a design of bionic alveolar remodelling microenvironment, that is in early time microvessels formed by b FGF provide nutritious to recruited endogenous stem cells, then TGFβ3 slowly released speed up the process of new bones formation to common facilitate rat alveolar defect repair. The DFH with higher regenerative efficiency dovetails nicely with great demand due to the requirement of complicated biological processes.
文摘目的:探讨浓缩生长因子纤维蛋白膜联合Bio-Oss骨粉修复拔牙术后牙槽骨缺损的疗效。方法:选择2018年1月-2020年1月笔者医院收治的因拔除下颌阻生第三磨牙致第二磨牙远中骨缺损患者76例,予以随机数字表法分为对照组(Bio-Oss骨粉植入,38例)和观察组(CGF膜联合Bio-Oss骨粉植入,38例)。手术后3 d复诊观察患者术后疼痛、开口度情况,比较两组患者术后1周创面愈合情况以及术前、术后1个月和3个月牙槽骨密度、牙槽嵴高度及牙槽嵴宽度,记录术后不良反应。结果:对照组术后3 d VAS评分(3.35±0.47)分,观察组为(2.67±0.42)分,对照组开口度(2.75±0.46)cm,观察组(2.68±0.51)cm,两组比较差异均无统计学意义(P>0.05)。术后1周,观察组创面愈合情况好于对照组,差异有统计学意义(P<0.05);术后1个月、术后3个月,观察组牙槽骨密度、牙槽嵴高度、牙槽嵴宽度均高于对照组,差异有统计学意义(P<0.05)。对照组术后不良反应发生率23.68%,高于观察组的5.26%,差异有统计学意义(P<0.05)。结论:浓缩生长因子纤维蛋白膜联合Bio-Oss骨粉在第二磨牙远中骨缺损修复中发挥着重要作用,值得临床推广应用。
