Heterotrophic ammonium removal characteristics of an aerobic heterotrophic nitrifying-denitrifying bacterium, Providencia rettgeri YL

Bacterium Providencia rettgeri YL was found to exhibit an unusual ability to heterotrophically nitrify and aerobically denitrify various concentrations of ammonium （NH4^＋-N）. In order to further understand its removal ability, several experiments were conducted to identify the growth and ammonium removal response at different carbon to nitrogen （C/N） mass ratios, shaking speeds, temperatures, ammonium concentrations and to qualitatively verify the production of nitrogen gas using gas chromatography techniques. Results showed that under optimum conditions （C/N 10, 30℃, 120 r/min）, YL can significantly remove low and high concentrations of ammonium within 12 to 48 h of growth, respectively. The nitrification products hydroxylamine （NHzOH）, nitrite （NO2^-） and nitrate （NO3^-） as well as the denitrification product, nitrogen gas （N2）, were detected under completely aerobic conditions.

Ammonium Removal from Ammonium Rich Solution by Bio Char

When biochar made from waste pallet was added to treated livestock wastewater, the total nitrogen and ammonium ion concentrations decreased, with removal rates over 10 days of about 60% and 30%, respectively. Bacteria were isolated with high ammonium removal ability and they were identified based on their 16S rRNA gene sequences. Anaerobic denitrifying Cronobacter spp. was isolated from the biochar used for water purification. When each strain was cultured in a liquid medium containing ammonium sulfate (initial ammonium concentration 30 mg/L), the highest ammonium removal rates were 83.8% - 96.5%. Organic acids were more effective than carbohydrates as sole carbon sources for nitrogen removal from wastewater. The absorbance at 660 nm increased with nitrogen removal, indicating that cells proliferated, so it was presumed that ammonium was taken up by assimilation.

A comparison study of the start-up of a MnO_x filter for catalytic oxidative removal of ammonium from groundwater and surface water

As an efficient method for ammonium（NH4~＋）removal,contact catalytic oxidation technology has drawn much attention recently,due to its good low temperature resistance and short start-up period.Two identical filters were employed to compare the process for ammonium removal during the start-up period for ammonium removal in groundwater（Filter-N）and surface water（Filter-S）treatment.Two types of source water（groundwater and surface water）were used as the feed waters for the filtration trials.Although the same initiating method was used,Filter-N exhibited much better ammonium removal performance than Filter-S.The differences in catalytic activity among these two filters were probed using X-ray diffraction（XRD）,scanning electron microscopy（SEM）,X-ray photoelectron spectroscopy（XPS）,and compositional analysis.XRD results indicated that different manganese oxide species were formed in Filter-N and Filter-S.Furthermore,the Mn3p XPS spectra taken on the surface of the filter films revealed that the average manganese valence of the inactive manganese oxide film collected from Filter-S（FS-MnOx）was higher than in the film collected from Filter-N（FN-MnOx）.Mn（IV）was identified as the predominant oxidation state in FS-MnOxand Mn（III）was identified as the predominant oxidation state in FN-MnOx.The results of compositional analyses suggested that polyaluminum ferric chloride（PAFC）used during the surface water treatment was an important factor in the mineralogy and reactivity of MnOx.This study provides the theoretical basis for promoting the wide application of the technology and has great practical significance.

Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass Micropterus salmoides culture pond

This work evaluates the application potential of a new indigenous aerobic denitrifi er, strain Pseudomonas CW-2, isolated from a largemouth bass culture pond. The rate of ammonium-N removal by strain CW-2 was approximately 97% at a DO concentration of 5.2 mg/L. Furthermore, when nitrate and ammonia coexisted, the strain gave priority to assimilating ammonia, and thereafter to denitrifi cation. Under optimal cultivation conditions, citrate and acetate were the carbon resources, C/N was 8, dissolved oxygen was 5.2 mg/L, and pH was 7; the removal rate of ammonium reached nearly 90%. The changing patterns of different bacteria in strain CW-2-treated and the control pond water were also compared. Lower levels of ammonia, nitrite, and phosphates were observed in the treated water as compared with the controls. Meanwhile, phylum-level distributions of the bacterial OTUs revealed that P roteobacteria, Bacteroidetes, Planctomycetes, and N itrospirae continuously changed their relative abundances in relation to carbon and the addition of strain CW-2; this finding implies that the conventional denitrifi cation process was weakened under the ef fects of carbon or the presence of strain CW-2. We propose that strain CW-2 is a promising organism for the removal of ammonium in intensive fish culture systems, according to our evaluations of its denitrifi cation performance.

Structural characteristic and ammonium and manganese catalytic activity of two types of filter media in groundwater treatment

Two types of filter media in groundwater treatment were conducted for a comparative study of surface structure and catalytic performance. Natural filter media was adopted from a conventional aeration–filtration groundwater treatment plant, and active filter media as a novel and promising filter media was also adopted. The physicochemical properties of these two kinds of filter media were characterized using numerous analytical techniques,such as X-Ray diffraction（XRD）, scanning electron microscope（SEM）, energy dispersive X-ray（EDX）, X-ray photoelectron spectroscopy（XPS） and Zeta potential. The catalytic activities of these filter media were evaluated for ammonium and manganese oxidation.XRD data showed that both active filter media and natural filter media belonged to birnessite family. A new manganese dioxide（Mn O2） phase（PDF#72-1982） was found in the structure of natural filter media. The SEM micrograph of natural filter media showed honeycomb structures and the active filter media presented plate structures and consisted of stacked particle. These natural filter media presented lower level of some trace elements such as calcium and magnesium, lower degree of crystallinity, lower Mn（III） content and lattice oxygen content than that of active filter media, which were associated with its poor ammonium and manganese catalytic activities. In addition, some γ-Fe2 O3 and Mn CO3 were found in the coating which may hinder the ammonium and manganese catalytic oxidation. This study provides a thorough and comprehensive understanding about the most commonly used filter media in water treatment, which can provide a theoretical guide to practical applications.