Objective To study the effects and the mechanisms of extract from a leguminous plant （Ammopiptanthus mongolicus cheng f.） （JAl ） in northwest China on inducing apoptosis and inhibiting proliferation of HepG2 hepat...Objective To study the effects and the mechanisms of extract from a leguminous plant （Ammopiptanthus mongolicus cheng f.） （JAl ） in northwest China on inducing apoptosis and inhibiting proliferation of HepG2 hepatocarcinoma cell in vitro. Methods The HepG2 cell line was used as target cells. The effect of 3A 1 on HepG2 cell growth was detected by microculture tetrazolium assay （MTr）, flow cytometry assay, DNA agarose gel electrophoresis and transmission electronic microscopy. The expressive effect of the wt-p53 in HepG2 cells was analyzed with p53 protein test-reagent. Results JAl not only had significant anti-proliferative effects depending upon time and dosage, but also induced apoptosis of HepG2 cells. Apoptotic typical morphological changes were observed in JAl-treated HepG2 cells under transmission electronic microscope, ＂Sub-G 1＂ phase peak occurred in flow cytometry and DNA ＂ladder＂ was found in DNA agarose gel electrophoresis. The expression of the wt-p53 increased in vitro, and 3Al-treated HepG2 and the positive cell percentage of the wt-p53 protein also increased. Conclusions JAl could obviously induce apoptosis and inhibit proliferation of HepG2 cells in vitro, and these effects are closely related with the increase of wt-p53 expression. JAl can be used as a good source of medicinal plant for the treatment of hepatocarcinoma.展开更多
沙冬青是亚洲荒漠唯一的常绿阔叶灌木,目前处于濒临灭绝的境地,准确掌握沙冬青的空间分布将有助于其生境保护和物种恢复。采用ASD Field Spec便携式光谱仪测定新疆沙冬青和蒙古沙冬青的叶片反射率,利用一阶导数和倒高斯模型提取沙冬青...沙冬青是亚洲荒漠唯一的常绿阔叶灌木,目前处于濒临灭绝的境地,准确掌握沙冬青的空间分布将有助于其生境保护和物种恢复。采用ASD Field Spec便携式光谱仪测定新疆沙冬青和蒙古沙冬青的叶片反射率,利用一阶导数和倒高斯模型提取沙冬青的高光谱特征,以探究冬季识别新疆沙冬青群落主要物种的可行性以及2种沙冬青的光谱特征差异。结果表明：① 1月,新疆沙冬青仍具有绿色植物的基本光谱特征,与其群落中的膜果麻黄和琵琶柴光谱反射率在近红外波段（780-1 300 nm）具有明显差异,反射率之差最高可达33%。因此,选择冬季进行沙冬青的遥感识别是可行的;②新疆沙冬青和蒙古沙冬青的光谱反射率曲线波形相似,＂红边＂位置分别为720 nm和719 nm;③倒高斯模型可很好地模拟红边区域的光谱特征,模拟结果表明2种沙冬青均存在＂蓝移＂现象,且新疆沙冬青的＂蓝移＂偏量大于蒙古沙冬青,说明引种环境对沙冬青的生长有胁迫。展开更多
基金This research was supported by the National Key Technologies R&D Program of China (No. 2001BA901A33)
文摘Objective To study the effects and the mechanisms of extract from a leguminous plant （Ammopiptanthus mongolicus cheng f.） （JAl ） in northwest China on inducing apoptosis and inhibiting proliferation of HepG2 hepatocarcinoma cell in vitro. Methods The HepG2 cell line was used as target cells. The effect of 3A 1 on HepG2 cell growth was detected by microculture tetrazolium assay （MTr）, flow cytometry assay, DNA agarose gel electrophoresis and transmission electronic microscopy. The expressive effect of the wt-p53 in HepG2 cells was analyzed with p53 protein test-reagent. Results JAl not only had significant anti-proliferative effects depending upon time and dosage, but also induced apoptosis of HepG2 cells. Apoptotic typical morphological changes were observed in JAl-treated HepG2 cells under transmission electronic microscope, ＂Sub-G 1＂ phase peak occurred in flow cytometry and DNA ＂ladder＂ was found in DNA agarose gel electrophoresis. The expression of the wt-p53 increased in vitro, and 3Al-treated HepG2 and the positive cell percentage of the wt-p53 protein also increased. Conclusions JAl could obviously induce apoptosis and inhibit proliferation of HepG2 cells in vitro, and these effects are closely related with the increase of wt-p53 expression. JAl can be used as a good source of medicinal plant for the treatment of hepatocarcinoma.
