Research progress on chemical constituents and pharmacological activities of Amomum longiligulare T.L.Wu

In order to develop and utilize the tropical medicinal resource Amomum(A.)longiligulare T.L.Wu more reasonably and effectively,this paper summarizes the research progress in chemical constituents and pharmacological activities of A.longiligulare by consulting the literatures,so as to provide a certain theoretical basis for exploring its material basis.The chemical constituents of A.longiligulare mainly include volatile oil,diphenylheptane and flavonoids,which have good efficacy in anti-peptic ulcer,as well as antioxidant,analgesic and other pharmacological activities.This paper briefly discusses the reasons for the differences in the composition of volatile oil studied by different scholars.By summarizing its pharmacological activities,it is found that its various pharmacological activities may be the basis of its anti-peptic ulcer.

Optimization of extraction process of Amomum longiligulare T.L.Wu polysaccharides by response surface methodology

Objective:To optimize the technological conditions of ultrasonic-assisted extraction of Amomum longiligulare T.L.Wu polysaccharides and increase the yield of polysaccharides.Methods:The polysaccharide was prepared by water extraction and alcohol precipitation method,and the yield of polysaccharide was taken as an index.The effects of ultrasonic time,extraction times,ultrasonic temperature,and water-to-material ratio on polysaccharide yield were investigated through a single factor experiment.Combined experimental design and response surface analysis were used to optimize the extraction process of Amomum longiligulare T.L.Wu polysaccharides.Results:The optimum conditions of ultrasonic-assisted extraction were determined as follows:extraction time of 29 min,three extraction times,water bath extraction temperature of 68℃,water-to-material ratio of 15:1;under these conditions,the polysaccharide yield was 10.69%.Conclusion:The results are close to the predicted values of the model.This optimization test is effective and feasible,and provides a reference for the related research of Amomum longiligulare T.L.Wu.

海南砂仁挥发油抗炎镇痛止泻的实验研究

目的:研究海南砂仁挥发油的抗炎、镇痛及止泻作用。方法:观察不同剂量的海南砂仁挥发油对二甲苯致小鼠耳肿胀和卡拉胶所致大鼠足肿胀的影响,对小鼠热板法痛阈值和冰醋酸所致小鼠扭体次数的影响以及对番泻叶和蓖麻油所致小鼠腹泻的影响。结果:海南砂仁挥发油对二甲苯致小鼠耳肿胀和卡拉胶所致大鼠足肿胀均有抑制作用,其中高剂量组作用与吲哚美辛相当。海南砂仁挥发油对热致痛小鼠可延长其痛阈时间,高剂量组较为明显;对醋酸致痛小鼠,各剂量组可减少其扭体次数,其作用无显著性差异。海南砂仁挥发油对番泻叶所致腹泻有效,对蓖麻油所致腹泻无效。结论:海南砂仁挥发油有一定的抗炎、镇痛和止泻作用,可能是其治疗溃疡性结肠炎的机理之一。

不同产地绿壳砂仁挥发油组分比较

目的比较7种绿壳砂仁挥发油化学成分。方法采用GC-MS技术结合NIST08数据库对7种绿壳砂仁挥发油进行分析鉴定,并用面积归一法确定各成分相对含有量。结果 7种绿壳砂仁挥发油化学组成及含有量各不相同。其中西双版纳产绿壳砂仁中乙酸龙脑酯含有量最高。结论不同产地的绿壳砂仁可能因生长环境不同,化学成分组成存在一定差异。以乙酸龙脑酯计西双版纳产绿壳砂仁品质最佳。

海南砂仁脂溶性成分的GC-MS分析

目的:研究海南砂仁的脂溶性成分。方法:利用溶剂分步萃取,得到各萃取部位;采用气相色谱-质谱(GC-MS)联用技术对其石油醚部位的成分进行测定,并与标准图谱对照,确定化合物成分,经色谱峰面积归一化法确定各成分的相对百分含量。结果:从海南砂仁石油醚萃取部位中共分离出76个组分,鉴定了其中的56种成分,所鉴定的成分占总脂溶性成分的68.81%,其中相对质量分数较高的成分有对苯二酚(7.74%)、Z,E-2,13-十八碳二烯-1-醇(6.86%)、E,E-2,13-十八碳二烯-1-醇(5.44%)、4-十一烷基苯酚(3.71%)、(Z)-9-十八碳烯醛(3.65%)、顺式-异油酸(3.39%)及(E,E)-大牻牛儿-3,7(11),9-三烯-6-酮(3.05%)等。结论:本研究首次对海南砂仁的石油醚部位的化学成分进行了分析,该研究为海南砂仁的进一步开发和利用提供了基础研究数据。

砂仁与代用品建砂仁的鉴别

目的论述砂仁与建砂仁的鉴别特征,为临床使用提供参考。方法查阅文献,从历代古书记录、来源、地理分布、外观性状、显微结构、化学成分及紫外光谱法等几个方面论述阳春砂仁,绿壳砂仁,海南砂仁与建砂仁的区别。结果砂仁与建砂仁的在来源方面、地理分布、外观性状、显微结构、化学成分以及药用功效等几个方面均有不同。结论根据植物来源、性状、显微理化鉴别的特征和紫外光谱法,可以明显的鉴别出砂仁与代用品建砂仁。

海南砂种苗繁育技术规程

从种子繁殖、分株繁殖和组培繁殖的育苗技术,以及种苗病虫害防治、种苗出圃和包装运输等方面,明确规定了海南砂的种子苗、分株苗和组培苗生产技术规程,为海南砂种苗标准化生产提供依据。

海南砂仁超临界萃取物中化学成分的GC-MS分析

目的研究海南砂仁超临界CO_2萃取物中的化学成分。方法采用超临界CO2流体萃取法提取海南砂仁挥发油,并通过气相色谱-质谱联用技术对其成分进行测定,结合计算机检索技术对分离的化合物进行鉴定,同时采用峰而积归一化法确定各成分的相对百分含量。结果从海南砂仁油中共分离出38个组分,鉴定了其中的35种成分,所鉴定的成分占总挥发油成分的95.55%,其中相对百分含量较高的有2-(3-羟基-4-甲氧基苯基)-3,7-二甲氧基-4H-苯并吡喃-4-酮(17.38%)、顺-9-十八碳烯醛(7.21%)、2-十一碳烯-10-十一碳炔酸酯(6.79%)、E,E,Z-1,3,12-十九碳三烯-5,14-二醇(5.97%)等。结论从海南砂仁萃取物中分离鉴定了35种成分,它们的含量占超临界萃取成分总面积的95.55%。