[Objectives]To study the antioxidant activity of the ethyl acetate extract of Amomum villosum Lour.[Methods]The removal rate of chelated iron ions,hydroxyl radicals,superoxide anion free radicals and DPPH free radical...[Objectives]To study the antioxidant activity of the ethyl acetate extract of Amomum villosum Lour.[Methods]The removal rate of chelated iron ions,hydroxyl radicals,superoxide anion free radicals and DPPH free radicals by the ethyl acetate extract of A.villosum Lour.was determined by UV spectrophotometer.[Results]0.5000μg/mL ethyl acetate extract of A.villosum Lour.had the strongest ability to chelate with ferrous ions and to remove hydroxyl radicals,superoxide anion free radicals and DPPH free radicals.The ability to chelate with ferrous ions was 95.14%,and the removal rate of the above free radicals was 86.217%,81.44%,and 85.16%.[Conclusions]The ethyl acetate extract of A.villosum Lour.had a strong antioxidant effect,and its antioxidant capacity was related to the sample concentration,which provides a theoretical basis for its application in the development of antioxidant skin care products.展开更多
In order to develop and utilize the tropical medicinal resource Amomum(A.)longiligulare T.L.Wu more reasonably and effectively,this paper summarizes the research progress in chemical constituents and pharmacological a...In order to develop and utilize the tropical medicinal resource Amomum(A.)longiligulare T.L.Wu more reasonably and effectively,this paper summarizes the research progress in chemical constituents and pharmacological activities of A.longiligulare by consulting the literatures,so as to provide a certain theoretical basis for exploring its material basis.The chemical constituents of A.longiligulare mainly include volatile oil,diphenylheptane and flavonoids,which have good efficacy in anti-peptic ulcer,as well as antioxidant,analgesic and other pharmacological activities.This paper briefly discusses the reasons for the differences in the composition of volatile oil studied by different scholars.By summarizing its pharmacological activities,it is found that its various pharmacological activities may be the basis of its anti-peptic ulcer.展开更多
Objective:To optimize the technological conditions of ultrasonic-assisted extraction of Amomum longiligulare T.L.Wu polysaccharides and increase the yield of polysaccharides.Methods:The polysaccharide was prepared by ...Objective:To optimize the technological conditions of ultrasonic-assisted extraction of Amomum longiligulare T.L.Wu polysaccharides and increase the yield of polysaccharides.Methods:The polysaccharide was prepared by water extraction and alcohol precipitation method,and the yield of polysaccharide was taken as an index.The effects of ultrasonic time,extraction times,ultrasonic temperature,and water-to-material ratio on polysaccharide yield were investigated through a single factor experiment.Combined experimental design and response surface analysis were used to optimize the extraction process of Amomum longiligulare T.L.Wu polysaccharides.Results:The optimum conditions of ultrasonic-assisted extraction were determined as follows:extraction time of 29 min,three extraction times,water bath extraction temperature of 68℃,water-to-material ratio of 15:1;under these conditions,the polysaccharide yield was 10.69%.Conclusion:The results are close to the predicted values of the model.This optimization test is effective and feasible,and provides a reference for the related research of Amomum longiligulare T.L.Wu.展开更多
Amomum villosum,Aquilaria sinensis,Morinda officinalis,and Pogostemon cablin are well-known medicinal plants in South China for their particular pharmacological activities,but their endophytic fungi have been rarely r...Amomum villosum,Aquilaria sinensis,Morinda officinalis,and Pogostemon cablin are well-known medicinal plants in South China for their particular pharmacological activities,but their endophytic fungi have been rarely reported.Here,the endophytic fungal communities associated with the leaves,stems,and roots of the four medicinal plant species were investigated by high-throughput sequencing technology.From the four medicinal plant species,a total of 169,149 sequences(reads)of endophytic fungi were harvested and clustered into 791 operational taxonomic units(OTUs)assigned to the Kingdom Fungi.These OTUs taxonomically spanned five phyla,27 classes,75 orders,167 families,and 291 genera.At the genus level,Phyllosticta,Candida,Zasmidium,and Cryptococcus were the major genera detected in A.villosum;Nigrospora,Tylopilus,Arthrobotrys,Coniosporium,and Corynespora were the dominant genera isolated in A.sinensis;Ochroconis,Cercospora,Aspergillus,and Cyphellophora were the dominant genera detected in M.officinalis;and Cladophialophora,Meira,Sakaguchia,and Penicillium were the major genera isolated in P.cablin.Among the four medicinal plant species,M.officinalis had the highest endophytic fungal diversity(H′>4.25),however A.sinensis had the lowest(H′<2.37).There were significant differences in endophytic fungal communities among different organs in the same plant species.展开更多
OBJECTIVE:This study was conducted to evaluate the effect of Amomum villosum on longitudinal bone growth.METHODS:Adolescent female Sprague-Dawley rats were divided into 3 groups and treated for 4 days:control(distille...OBJECTIVE:This study was conducted to evaluate the effect of Amomum villosum on longitudinal bone growth.METHODS:Adolescent female Sprague-Dawley rats were divided into 3 groups and treated for 4 days:control(distilled water,p.o.),recombinant human growth hormone(rhGH;100 μg/kg,s.c.),and A.villosum(500 mg/kg,p.o.) groups.On day 3,tetracycline(20 g/kg,i.p.) was injected for growth plate identification.On days 2,3 and 4,5-bromo-2'-deoxyuridine(BrdU)(50 mg/kg,i.p.) was injected to label proliferating cells.On day 5,tibias were dissected and fixed in 4% paraformaldehyde,dehydrated,and sectioned for immunohistochemistry and histomorphometry.RESULTS:The rate of bone growth in the A.villosum and rhGH groups increased to(410 ± 44) and(389 ± 46) μm/day(P<0.01),respectively,as compared with the control(330.7 ± 34.7) μm/day.The thickness of the growth plates also increased to(591 ± 37) and(598 ± 32) μm,respectively,as compared with the control(524± 89) μm(P<0.001).The number of BrdU-positive cells in the chondrocytes of the A.villosum and rhGH groups was also significantly higher(126±24) and(143±18) cells/mm 2,respectively) than in the control(109 ± 25) mm 2(P<0.05).Insulin-like growth factor-1 and bone morphogenetic protein-2 in the A.villosum and rhGH groups were highly expressed in the growth plate as compared with the control samples,indicating increased bone formation.CONCLUSIONS:A.villosum could be used to treat growth retardation during adolescence.展开更多
基金Supported by the Innovation and Entrepreneurship Training Planning Project for University Students in Guangxi Autonomous Region(S202210599128X).
文摘[Objectives]To study the antioxidant activity of the ethyl acetate extract of Amomum villosum Lour.[Methods]The removal rate of chelated iron ions,hydroxyl radicals,superoxide anion free radicals and DPPH free radicals by the ethyl acetate extract of A.villosum Lour.was determined by UV spectrophotometer.[Results]0.5000μg/mL ethyl acetate extract of A.villosum Lour.had the strongest ability to chelate with ferrous ions and to remove hydroxyl radicals,superoxide anion free radicals and DPPH free radicals.The ability to chelate with ferrous ions was 95.14%,and the removal rate of the above free radicals was 86.217%,81.44%,and 85.16%.[Conclusions]The ethyl acetate extract of A.villosum Lour.had a strong antioxidant effect,and its antioxidant capacity was related to the sample concentration,which provides a theoretical basis for its application in the development of antioxidant skin care products.
基金supported by the National Natural Science Foundation of China(81660649),and Innovative Scientific Research Project for Postgraduates of Hainan Medical University(HYYS2020-05)。
文摘In order to develop and utilize the tropical medicinal resource Amomum(A.)longiligulare T.L.Wu more reasonably and effectively,this paper summarizes the research progress in chemical constituents and pharmacological activities of A.longiligulare by consulting the literatures,so as to provide a certain theoretical basis for exploring its material basis.The chemical constituents of A.longiligulare mainly include volatile oil,diphenylheptane and flavonoids,which have good efficacy in anti-peptic ulcer,as well as antioxidant,analgesic and other pharmacological activities.This paper briefly discusses the reasons for the differences in the composition of volatile oil studied by different scholars.By summarizing its pharmacological activities,it is found that its various pharmacological activities may be the basis of its anti-peptic ulcer.
基金National Natural Science Foundation of China(No.81660649)Hainan Medical College Student Innovation and Entrepreneurship Training Program Project(No.201811810007)。
文摘Objective:To optimize the technological conditions of ultrasonic-assisted extraction of Amomum longiligulare T.L.Wu polysaccharides and increase the yield of polysaccharides.Methods:The polysaccharide was prepared by water extraction and alcohol precipitation method,and the yield of polysaccharide was taken as an index.The effects of ultrasonic time,extraction times,ultrasonic temperature,and water-to-material ratio on polysaccharide yield were investigated through a single factor experiment.Combined experimental design and response surface analysis were used to optimize the extraction process of Amomum longiligulare T.L.Wu polysaccharides.Results:The optimum conditions of ultrasonic-assisted extraction were determined as follows:extraction time of 29 min,three extraction times,water bath extraction temperature of 68℃,water-to-material ratio of 15:1;under these conditions,the polysaccharide yield was 10.69%.Conclusion:The results are close to the predicted values of the model.This optimization test is effective and feasible,and provides a reference for the related research of Amomum longiligulare T.L.Wu.
基金supported by the National 973 Preliminary Project(No.2014CB460613)the Field Scientific Experimental Station Project of Guangdong academy of science(No.Sytz201512)+1 种基金the Science and Technology Key Program of Guangzhou,China(201607020017)the Science and Technology Planning Project of Guangdong Province(Nos.2014A030304050 and 2015A030302060).
文摘Amomum villosum,Aquilaria sinensis,Morinda officinalis,and Pogostemon cablin are well-known medicinal plants in South China for their particular pharmacological activities,but their endophytic fungi have been rarely reported.Here,the endophytic fungal communities associated with the leaves,stems,and roots of the four medicinal plant species were investigated by high-throughput sequencing technology.From the four medicinal plant species,a total of 169,149 sequences(reads)of endophytic fungi were harvested and clustered into 791 operational taxonomic units(OTUs)assigned to the Kingdom Fungi.These OTUs taxonomically spanned five phyla,27 classes,75 orders,167 families,and 291 genera.At the genus level,Phyllosticta,Candida,Zasmidium,and Cryptococcus were the major genera detected in A.villosum;Nigrospora,Tylopilus,Arthrobotrys,Coniosporium,and Corynespora were the dominant genera isolated in A.sinensis;Ochroconis,Cercospora,Aspergillus,and Cyphellophora were the dominant genera detected in M.officinalis;and Cladophialophora,Meira,Sakaguchia,and Penicillium were the major genera isolated in P.cablin.Among the four medicinal plant species,M.officinalis had the highest endophytic fungal diversity(H′>4.25),however A.sinensis had the lowest(H′<2.37).There were significant differences in endophytic fungal communities among different organs in the same plant species.
基金Supported by the Fund of Amomum villosum in bone growth
文摘OBJECTIVE:This study was conducted to evaluate the effect of Amomum villosum on longitudinal bone growth.METHODS:Adolescent female Sprague-Dawley rats were divided into 3 groups and treated for 4 days:control(distilled water,p.o.),recombinant human growth hormone(rhGH;100 μg/kg,s.c.),and A.villosum(500 mg/kg,p.o.) groups.On day 3,tetracycline(20 g/kg,i.p.) was injected for growth plate identification.On days 2,3 and 4,5-bromo-2'-deoxyuridine(BrdU)(50 mg/kg,i.p.) was injected to label proliferating cells.On day 5,tibias were dissected and fixed in 4% paraformaldehyde,dehydrated,and sectioned for immunohistochemistry and histomorphometry.RESULTS:The rate of bone growth in the A.villosum and rhGH groups increased to(410 ± 44) and(389 ± 46) μm/day(P<0.01),respectively,as compared with the control(330.7 ± 34.7) μm/day.The thickness of the growth plates also increased to(591 ± 37) and(598 ± 32) μm,respectively,as compared with the control(524± 89) μm(P<0.001).The number of BrdU-positive cells in the chondrocytes of the A.villosum and rhGH groups was also significantly higher(126±24) and(143±18) cells/mm 2,respectively) than in the control(109 ± 25) mm 2(P<0.05).Insulin-like growth factor-1 and bone morphogenetic protein-2 in the A.villosum and rhGH groups were highly expressed in the growth plate as compared with the control samples,indicating increased bone formation.CONCLUSIONS:A.villosum could be used to treat growth retardation during adolescence.
