In this paper,the ultrasonic-assisted extraction process of flavonoid compounds from leaves of Amorpha fruticosa is optimized.In single factor experiments,solid/liquid ratios,ultrasonic power,ethanol concentrations an...In this paper,the ultrasonic-assisted extraction process of flavonoid compounds from leaves of Amorpha fruticosa is optimized.In single factor experiments,solid/liquid ratios,ultrasonic power,ethanol concentrations and extraction cycles were experimental factors.Box–Behnken central composite design and RSM analyzed the effects of the four factors on the yield of total flavonoids.The optimal extraction parameters were solid/liquid ratio 1:50 g/mL,ultrasonic power 316 W,ethanol concentration 50%,4 extraction cycles.In the optimized condition,the estimated value of the regression model was 66.6456 mg/g while the measured value was 66.4329 mg/g.展开更多
We investigated the combined effects of soil moisture and light intensity on the growth, development and ecophysiological characteristics of one-year old Amorpha fruticosa seedlings. Soil moisture and light intensity ...We investigated the combined effects of soil moisture and light intensity on the growth, development and ecophysiological characteristics of one-year old Amorpha fruticosa seedlings. Soil moisture and light intensity influenced the ecophysiological characteristics of Amorpha fruticosa seedlings. Soil moisture resulted in the decreases of growth rate, individual size, net photosynthetic rate, transpiration rate, leaf water loss rate （WLR）, and biomass accumulation of plant parts, and led to increased leaf water saturation deficit （WSD）. Under water stress, more photosynthetic products were allocated to root growth. With decreasing light intensity, net photosynthetic rate, transpiration rate, chla/b, water saturation deficit, water use efficiency, water loss rate and biomass accumulation declined, while Chla, Chlb, Chla＋b and carotenoids （Car） increased and more photosynthetic products were allocated to stem and leaf growth. Maximum growth vigor, net photosynthetic rate and total biomass accumulation in Amorpha fruticosa seedlings was recorded at 75 80% soil water-holding capacity and 100% light density in greenhouse environments.展开更多
A new isoflavone was obstained from the medicinal herb Amorpha fruticosa. It was elucidated asbenzopyran- 12-one, 1,4,10,11 - tetrahydro-6′-[8′-（hydroxymethy1）etheny1]-2,3-dimethoxy-8′-O-β-glucopyranosy1-O-α-D-...A new isoflavone was obstained from the medicinal herb Amorpha fruticosa. It was elucidated asbenzopyran- 12-one, 1,4,10,11 - tetrahydro-6′-[8′-（hydroxymethy1）etheny1]-2,3-dimethoxy-8′-O-β-glucopyranosy1-O-α-D-arabinoside by spectroscopic methods including UV, IR, 1D NMR and 2D NMR techniques. And the activity against acetaminophen-induced hepatotoxicity of this compound was also studied, and found this compound can protect liver obviously from hepatotoxicity induced by acetaminophen （AAP）. ？2009 Ting Guo Kang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.展开更多
A method based on degenerate Oligo primed polymerase chain reaction (PCR) and random amplification of cDNA end (RACE) PCR for cloning a full length cDNA is described. An Amorpha fruticosa cDNA clone encoding UDP gluco...A method based on degenerate Oligo primed polymerase chain reaction (PCR) and random amplification of cDNA end (RACE) PCR for cloning a full length cDNA is described. An Amorpha fruticosa cDNA clone encoding UDP glucose pyrophosphorylase (UGP), a key enzyme producing UDP glucose in the synthesis of sucrose and cellulose, is cloned by using this method. We design 5’ RACE primers based on UGPA1 fragment, which obtains from degenerate PCR. Inverse PCR and nested PCR enable cloning of the remainder 5’ and 3’ end fragments of the gene. The deduced amino acid sequence exhibits significant homology with the other UGP genes cloned. This method is more simple and inexpensive than screening cDNA library, and can be easily adapted to clone other genes.展开更多
基金supported by Central University Basic Research Funds(2572014CA27),(2572018DB01)Heilongjiang Province Natural Fund(C200913)
文摘In this paper,the ultrasonic-assisted extraction process of flavonoid compounds from leaves of Amorpha fruticosa is optimized.In single factor experiments,solid/liquid ratios,ultrasonic power,ethanol concentrations and extraction cycles were experimental factors.Box–Behnken central composite design and RSM analyzed the effects of the four factors on the yield of total flavonoids.The optimal extraction parameters were solid/liquid ratio 1:50 g/mL,ultrasonic power 316 W,ethanol concentration 50%,4 extraction cycles.In the optimized condition,the estimated value of the regression model was 66.6456 mg/g while the measured value was 66.4329 mg/g.
基金supported by National Science Foundation of China (No.31270374)Independent Innovation Foundation of Shandong University (No.2011DX008)+1 种基金Natural Science Foundation of Shandong Province,China (No.2009ZRB01875ZR2010CM062)
文摘We investigated the combined effects of soil moisture and light intensity on the growth, development and ecophysiological characteristics of one-year old Amorpha fruticosa seedlings. Soil moisture and light intensity influenced the ecophysiological characteristics of Amorpha fruticosa seedlings. Soil moisture resulted in the decreases of growth rate, individual size, net photosynthetic rate, transpiration rate, leaf water loss rate （WLR）, and biomass accumulation of plant parts, and led to increased leaf water saturation deficit （WSD）. Under water stress, more photosynthetic products were allocated to root growth. With decreasing light intensity, net photosynthetic rate, transpiration rate, chla/b, water saturation deficit, water use efficiency, water loss rate and biomass accumulation declined, while Chla, Chlb, Chla＋b and carotenoids （Car） increased and more photosynthetic products were allocated to stem and leaf growth. Maximum growth vigor, net photosynthetic rate and total biomass accumulation in Amorpha fruticosa seedlings was recorded at 75 80% soil water-holding capacity and 100% light density in greenhouse environments.
基金supported by the Science and Technology of Dalian(No.2008E11SF168).
文摘A new isoflavone was obstained from the medicinal herb Amorpha fruticosa. It was elucidated asbenzopyran- 12-one, 1,4,10,11 - tetrahydro-6′-[8′-（hydroxymethy1）etheny1]-2,3-dimethoxy-8′-O-β-glucopyranosy1-O-α-D-arabinoside by spectroscopic methods including UV, IR, 1D NMR and 2D NMR techniques. And the activity against acetaminophen-induced hepatotoxicity of this compound was also studied, and found this compound can protect liver obviously from hepatotoxicity induced by acetaminophen （AAP）. ？2009 Ting Guo Kang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
文摘A method based on degenerate Oligo primed polymerase chain reaction (PCR) and random amplification of cDNA end (RACE) PCR for cloning a full length cDNA is described. An Amorpha fruticosa cDNA clone encoding UDP glucose pyrophosphorylase (UGP), a key enzyme producing UDP glucose in the synthesis of sucrose and cellulose, is cloned by using this method. We design 5’ RACE primers based on UGPA1 fragment, which obtains from degenerate PCR. Inverse PCR and nested PCR enable cloning of the remainder 5’ and 3’ end fragments of the gene. The deduced amino acid sequence exhibits significant homology with the other UGP genes cloned. This method is more simple and inexpensive than screening cDNA library, and can be easily adapted to clone other genes.
