Objective:Amphenicols(chloramphenicol,thiamphenicol and forfenicol)can cause aplastic anaemia and other severe side effects to consumers;therefore,it is necessary to inspect their residues in foods of animal origin.Ho...Objective:Amphenicols(chloramphenicol,thiamphenicol and forfenicol)can cause aplastic anaemia and other severe side effects to consumers;therefore,it is necessary to inspect their residues in foods of animal origin.However,there has been no report on the use of amphenicols receptor for the determination of their residues,and none of the previously reported immunoassays for amphenicols can differentiate the specifc species.Materials and Methods:In this study,the ribosomal protein L16 of Escherichia coli was frst expressed,and its intermolecular interaction mechanisms with the three amphenicols was studied using the molecular docking technique.The protein was then combined with three enzymelabelled conjugates to develop a direct competitive array on microplate for determination of the three drugs in egg.Results:Due to the use of principal component analysis to analyse the data,this method could discriminate the three drugs in the range 0.1–10 ng/mL,and the limits of detection for the three drugs were in the range of 0.0002–0.0009 ng/mL.The analysis results for the unknown egg samples were consistent with a liquid chromatography–tandem mass spectrometry method,and the method performances were superior to the previous immunoassays for amphenicols.Conclusion:This is the frst paper reporting the use of ribosomal protein L16 to develop a competitive array for discriminative determination of amphenicols in food samples.展开更多
建立了超高效液相色谱-串联质谱(UPLC-MS/MS)法同时测定水产品中11种酰胺醇和抗病毒类药物残留的检测方法。选择鱼、虾、鳖和贝类作为分析对象,样品采用4%(w/w)NaCl溶液分散基质后,2%(V/V)氨水-乙酸乙酯溶液萃取,提取液浓缩后残留物用80...建立了超高效液相色谱-串联质谱(UPLC-MS/MS)法同时测定水产品中11种酰胺醇和抗病毒类药物残留的检测方法。选择鱼、虾、鳖和贝类作为分析对象,样品采用4%(w/w)NaCl溶液分散基质后,2%(V/V)氨水-乙酸乙酯溶液萃取,提取液浓缩后残留物用80%(V/V)乙腈(含0.2%(V/V)甲酸)复溶,过Oasis PRIME HLB小柱净化后再次浓缩并上机分析。选择ZORBAX RRHD Eclipse Plus C18色谱柱,乙腈与5 mmol/L甲酸铵水溶液为流动相梯度洗脱,正、负源切换多反应监测模式采集质谱数据,同位素内标法定量。结果表明,11种待测药物在一定范围内线性良好,线性相关系数(r2)不低于0.996,方法的定量限为0.20~2.0μg/kg,加标回收率为84.6%~110%,相对标准偏差为1.0%~14%。方法适用于水产品中酰胺醇和抗病毒类药物的痕量分析,并成功用于水产品质量安全风险监测工作。展开更多
基金the National Natural Science Foundation of China(No.32372447)the Natural Science Foundation of Hebei Province(No.C2023204045),China.
文摘Objective:Amphenicols(chloramphenicol,thiamphenicol and forfenicol)can cause aplastic anaemia and other severe side effects to consumers;therefore,it is necessary to inspect their residues in foods of animal origin.However,there has been no report on the use of amphenicols receptor for the determination of their residues,and none of the previously reported immunoassays for amphenicols can differentiate the specifc species.Materials and Methods:In this study,the ribosomal protein L16 of Escherichia coli was frst expressed,and its intermolecular interaction mechanisms with the three amphenicols was studied using the molecular docking technique.The protein was then combined with three enzymelabelled conjugates to develop a direct competitive array on microplate for determination of the three drugs in egg.Results:Due to the use of principal component analysis to analyse the data,this method could discriminate the three drugs in the range 0.1–10 ng/mL,and the limits of detection for the three drugs were in the range of 0.0002–0.0009 ng/mL.The analysis results for the unknown egg samples were consistent with a liquid chromatography–tandem mass spectrometry method,and the method performances were superior to the previous immunoassays for amphenicols.Conclusion:This is the frst paper reporting the use of ribosomal protein L16 to develop a competitive array for discriminative determination of amphenicols in food samples.
文摘建立了超高效液相色谱-串联质谱(UPLC-MS/MS)法同时测定水产品中11种酰胺醇和抗病毒类药物残留的检测方法。选择鱼、虾、鳖和贝类作为分析对象,样品采用4%(w/w)NaCl溶液分散基质后,2%(V/V)氨水-乙酸乙酯溶液萃取,提取液浓缩后残留物用80%(V/V)乙腈(含0.2%(V/V)甲酸)复溶,过Oasis PRIME HLB小柱净化后再次浓缩并上机分析。选择ZORBAX RRHD Eclipse Plus C18色谱柱,乙腈与5 mmol/L甲酸铵水溶液为流动相梯度洗脱,正、负源切换多反应监测模式采集质谱数据,同位素内标法定量。结果表明,11种待测药物在一定范围内线性良好,线性相关系数(r2)不低于0.996,方法的定量限为0.20~2.0μg/kg,加标回收率为84.6%~110%,相对标准偏差为1.0%~14%。方法适用于水产品中酰胺醇和抗病毒类药物的痕量分析,并成功用于水产品质量安全风险监测工作。
