Objective:Angelica(A.)sinensis is used as a traditional medical herb for the treatment of neurodegeneration,aging,and inflammation in Asia.A.sinensis optimal formula(AOF)is the best combination in A.sinensis that has ...Objective:Angelica(A.)sinensis is used as a traditional medical herb for the treatment of neurodegeneration,aging,and inflammation in Asia.A.sinensis optimal formula(AOF)is the best combination in A.sinensis that has been screened to rescue the cognitive ability in P-amyloid peptide(Ap25-35)-treated Alzheimer’s disease(AD)rats.The objective of this study was to investigate the effect of AOF on the learning and memory of AD rats as well as to explore the underlying mechanisms.展开更多
Background We showed in our previous study that the N-terminal 17-mer peptide of amyloid precursor protein （APP17-mer peptide）,an active peptide segment with trophic and antioxidative effects,protects skin fibroblas...Background We showed in our previous study that the N-terminal 17-mer peptide of amyloid precursor protein （APP17-mer peptide）,an active peptide segment with trophic and antioxidative effects,protects skin fibroblasts against ultraviolet （UV） damage and downregulates matrix metalloproteinase 1 （MMP-1） expression.The aim of the current study was to explore the protective effects of P165,the N-terminal 5-mer peptide analog of amyloid precursor protein that is resistant to enzymolysis,on UVA-induced damage in human dermal fibroblasts （HDFs）.Methods HDFs were cultured in Dulbecco＆#39;s modified Eagle＆#39;s medium without and with P165 （concentrations were 1,10,and 100 μJmol/L）.Then,15 J/cm2 UVA irradiation was used to obtain the UV-irradiated model.Cell proliferation was analyzed using MTT kit.The collagen type Ⅰ and MMP-1 contents in cell lysate were determined by enzyme-linked immunosorbent assay （ELISA）.Fluorometric assays were performed to detect the formation of intracellular reactive oxygen species （ROS） in the cells.Results P165 significantly protected the HDFs against UVA-induced cytotoxicity.Compared with the UVA-irradiated control,1,10,and 100 μmol/L P165 elevated cell proliferation by 14.98% （P〈0.05）,17.52% （P〈0.01） and 28.34% （P〈0.001）,respectively.Simultaneously,10 and 100 μmol/L P165 increased collagen type Ⅰ content （both P〈0.05）.Moreover,P165 treatment （all concentrations） also markedly suppressed the UVA-induced MMP-1 expression （all P〈0.001）.P165 at 1,10,and 100 μmol/L also reduced UVA-induced ROS generation by 11.27%,13.69% （both P〈0.05）,and 25.48% （P〈0.001）,respectively.Conclusions P165 could protect the HDFs against UVA-induced photodamage,including cytotoxicity,and MMP-1 generation.Furthermore,it also increased the collagen type Ⅰ content in the cells.The inhibitory effect on intracellular ROS generation might be involved in these photoprotective effects.Thus,P165 may be a useful candidate in the prevention and treatment of skin photoaging.展开更多
Serum amyloid P component(SAP)is present in seminal plasma,on spermatozoa,and in different tissues of the male reproductive tract,but its function is not known.The aims of this study were to determine if the concentra...Serum amyloid P component(SAP)is present in seminal plasma,on spermatozoa,and in different tissues of the male reproductive tract,but its function is not known.The aims of this study were to determine if the concentration of SAP in seminal plasma is associated with commonly assessed semen parameters and to investigate if SAP could be a new,indirect biomarker for these parameters.In a cross-sectional study of 203 young volunteers,the concentration of SAP in seminal plasma was measured with a in-house developed enzyme-linked immunosorbent assay.Scatter plots,Pearson’s correlation coefficients(r),and linear regression models were produced,and SAP showed a statistically significant correlation with sperm concentration(r=0.75),sperm number(r=0.68),semen volume(r=-0.19),progressive sperm motility(r=0.24),and sperm immotility(r=-0.20).When the study group was dichotomized,SAP could be used to discriminate samples with a sperm concentration<or≥5×10^(6)ml^(-1),15×10^(6)ml^(-1),or 40×10^(6)ml^(-1),and in receiver operating characteristic curves,the corresponding areas under the curves were 0.97,0.93,and 0.82,respectively,with P<0.001 for all three cutoff values studied.The concentration of SAP in seminal plasma showed a strong,positive correlation with the concentration of spermatozoa in semen.SAP may be used as a new indirect potential biomarker for sperm concentration in fresh and in frozen,stored samples.In addition,it is envisaged that the assay could be developed into a home fertility test to differentiate between a low and a normal sperm concentration.展开更多
基金This project was supported by the National Natural Science Foundation of China(No.81960828).
文摘Objective:Angelica(A.)sinensis is used as a traditional medical herb for the treatment of neurodegeneration,aging,and inflammation in Asia.A.sinensis optimal formula(AOF)is the best combination in A.sinensis that has been screened to rescue the cognitive ability in P-amyloid peptide(Ap25-35)-treated Alzheimer’s disease(AD)rats.The objective of this study was to investigate the effect of AOF on the learning and memory of AD rats as well as to explore the underlying mechanisms.
基金This work was supported in part by a grant from the National Natural Science Foundation of China
文摘Background We showed in our previous study that the N-terminal 17-mer peptide of amyloid precursor protein （APP17-mer peptide）,an active peptide segment with trophic and antioxidative effects,protects skin fibroblasts against ultraviolet （UV） damage and downregulates matrix metalloproteinase 1 （MMP-1） expression.The aim of the current study was to explore the protective effects of P165,the N-terminal 5-mer peptide analog of amyloid precursor protein that is resistant to enzymolysis,on UVA-induced damage in human dermal fibroblasts （HDFs）.Methods HDFs were cultured in Dulbecco＆#39;s modified Eagle＆#39;s medium without and with P165 （concentrations were 1,10,and 100 μJmol/L）.Then,15 J/cm2 UVA irradiation was used to obtain the UV-irradiated model.Cell proliferation was analyzed using MTT kit.The collagen type Ⅰ and MMP-1 contents in cell lysate were determined by enzyme-linked immunosorbent assay （ELISA）.Fluorometric assays were performed to detect the formation of intracellular reactive oxygen species （ROS） in the cells.Results P165 significantly protected the HDFs against UVA-induced cytotoxicity.Compared with the UVA-irradiated control,1,10,and 100 μmol/L P165 elevated cell proliferation by 14.98% （P〈0.05）,17.52% （P〈0.01） and 28.34% （P〈0.001）,respectively.Simultaneously,10 and 100 μmol/L P165 increased collagen type Ⅰ content （both P〈0.05）.Moreover,P165 treatment （all concentrations） also markedly suppressed the UVA-induced MMP-1 expression （all P〈0.001）.P165 at 1,10,and 100 μmol/L also reduced UVA-induced ROS generation by 11.27%,13.69% （both P〈0.05）,and 25.48% （P〈0.001）,respectively.Conclusions P165 could protect the HDFs against UVA-induced photodamage,including cytotoxicity,and MMP-1 generation.Furthermore,it also increased the collagen type Ⅰ content in the cells.The inhibitory effect on intracellular ROS generation might be involved in these photoprotective effects.Thus,P165 may be a useful candidate in the prevention and treatment of skin photoaging.
文摘Serum amyloid P component(SAP)is present in seminal plasma,on spermatozoa,and in different tissues of the male reproductive tract,but its function is not known.The aims of this study were to determine if the concentration of SAP in seminal plasma is associated with commonly assessed semen parameters and to investigate if SAP could be a new,indirect biomarker for these parameters.In a cross-sectional study of 203 young volunteers,the concentration of SAP in seminal plasma was measured with a in-house developed enzyme-linked immunosorbent assay.Scatter plots,Pearson’s correlation coefficients(r),and linear regression models were produced,and SAP showed a statistically significant correlation with sperm concentration(r=0.75),sperm number(r=0.68),semen volume(r=-0.19),progressive sperm motility(r=0.24),and sperm immotility(r=-0.20).When the study group was dichotomized,SAP could be used to discriminate samples with a sperm concentration<or≥5×10^(6)ml^(-1),15×10^(6)ml^(-1),or 40×10^(6)ml^(-1),and in receiver operating characteristic curves,the corresponding areas under the curves were 0.97,0.93,and 0.82,respectively,with P<0.001 for all three cutoff values studied.The concentration of SAP in seminal plasma showed a strong,positive correlation with the concentration of spermatozoa in semen.SAP may be used as a new indirect potential biomarker for sperm concentration in fresh and in frozen,stored samples.In addition,it is envisaged that the assay could be developed into a home fertility test to differentiate between a low and a normal sperm concentration.
