Polyvinyl alcohol( PVA) is a water-soluble synthetic polymer that is hard to biodegrade. PVA-degrading microorganisms were previously reported as unitary bacteria and most of them have been identified as aerobes. In t...Polyvinyl alcohol( PVA) is a water-soluble synthetic polymer that is hard to biodegrade. PVA-degrading microorganisms were previously reported as unitary bacteria and most of them have been identified as aerobes. In this work,a microbial community was cultured anaerobically and its degradation performance and biodiversity were analyzed. The microbial community was cultured for more than 40 d,which represents a highly efficient degradation performance with a chemical oxygen demand removal efficiency of 88. 48%. Operational taxonomic unit-based analysis of the sequences revealed a highly diverse community in the reactor. To note,metagenome 16s rDNA sequencing delineated 19 phyla and 41 classes. Specifically, proteobacteria, chlamydiae, bacteroidetes,firmicutes,and planctomycetes play key roles in the biodegradation processes. Moreover,the betaproteobacteria class belonging to the proteobacteria phylum was the predominant bacterial members in this community. Our results demonstrated that anaerobic treatment of PVA wastewater is feasible and confers degradation by a highly diverse microbial community.展开更多
It has long been known that abundant symbiotic bacteria exist in the tunic and gut of marine ascidians,and that these play crucial roles in host development,physiological metabolism,and environmental adaptation.Howeve...It has long been known that abundant symbiotic bacteria exist in the tunic and gut of marine ascidians,and that these play crucial roles in host development,physiological metabolism,and environmental adaptation.However,the identity,roles and functions of these symbiotic bacteria are known for only a few strains.In this study,we isolated and cultivated 263 strains of microorganisms from the intestine of the marine ascidian Halocynthia roretzi through a combination of aerobic and anaerobic culture approaches.Most cultivated species,both aerobic and anaerobic,from ascidian stool samples belonged to the genus Bacillus based on 16S rDNA sequencing identification and phylogenetic assays.The distribution of cultured bacteria varied with seasonal changes in environmental conditions.To explore the functions of cultured bacteria,we screened out a strain of Serratia sp.whose extracts showed high antibacterial activity against aquatic pathogens.These findings revealed the potential roles of gut microorganisms in ascidian defense and environmental adaptation,thus providing insights into the interaction and co-evolution between gut bacteria and their hosts.展开更多
基金Science and Technology Plan of Henan Province,China(No.132300410080)North China University of Water Resources and Electric Power Postgraduate Education Innovation Program,China(No.YK2015-13)
文摘Polyvinyl alcohol( PVA) is a water-soluble synthetic polymer that is hard to biodegrade. PVA-degrading microorganisms were previously reported as unitary bacteria and most of them have been identified as aerobes. In this work,a microbial community was cultured anaerobically and its degradation performance and biodiversity were analyzed. The microbial community was cultured for more than 40 d,which represents a highly efficient degradation performance with a chemical oxygen demand removal efficiency of 88. 48%. Operational taxonomic unit-based analysis of the sequences revealed a highly diverse community in the reactor. To note,metagenome 16s rDNA sequencing delineated 19 phyla and 41 classes. Specifically, proteobacteria, chlamydiae, bacteroidetes,firmicutes,and planctomycetes play key roles in the biodegradation processes. Moreover,the betaproteobacteria class belonging to the proteobacteria phylum was the predominant bacterial members in this community. Our results demonstrated that anaerobic treatment of PVA wastewater is feasible and confers degradation by a highly diverse microbial community.
基金supported by the National Key Research and Development Program of China(Grant no.2018YFD0900705)the National Natural Science Foundation of China(Grant no.31970487).
文摘It has long been known that abundant symbiotic bacteria exist in the tunic and gut of marine ascidians,and that these play crucial roles in host development,physiological metabolism,and environmental adaptation.However,the identity,roles and functions of these symbiotic bacteria are known for only a few strains.In this study,we isolated and cultivated 263 strains of microorganisms from the intestine of the marine ascidian Halocynthia roretzi through a combination of aerobic and anaerobic culture approaches.Most cultivated species,both aerobic and anaerobic,from ascidian stool samples belonged to the genus Bacillus based on 16S rDNA sequencing identification and phylogenetic assays.The distribution of cultured bacteria varied with seasonal changes in environmental conditions.To explore the functions of cultured bacteria,we screened out a strain of Serratia sp.whose extracts showed high antibacterial activity against aquatic pathogens.These findings revealed the potential roles of gut microorganisms in ascidian defense and environmental adaptation,thus providing insights into the interaction and co-evolution between gut bacteria and their hosts.
