The aim of the present work was to evaluate the anaerobic ammonium oxidation （anammox） activity of simultaneous partial nitrification, anammox and denitrification （SNAD） biofilm with different substrate concentrat...The aim of the present work was to evaluate the anaerobic ammonium oxidation （anammox） activity of simultaneous partial nitrification, anammox and denitrification （SNAD） biofilm with different substrate concentrations and pH values. Kaldnes rings taken from the SNAD biofilm reactor were incubated in batch tests to determine the anammox activity. Haldane model was applied to investigate the ammonium inhibition on anammox process. As for nitrite inhibition, the NH4-N removal rate of anammox process remained 87.4% of the maximum rate with the NO2-N concentration of 100 mg/L. Based on the results of Haldane model, no obvious difference in kinetic coefficients was observed under high or low free ammonia （FA） conditions, indicating that ammonium rather than FA was the true inhibitor for anammox process of SNAD biofilm. With the pH value of 7.0, the rmax, KS and K of ammonium were 0.209 kg NO2-N/kg VSS/day, 9.5 mg/L and 422 rag/L, respectively. The suitable pH ranges for anammox process were B.0 to 9.0. These results indicate that the SNAD biofilm performs excellent tolerance to adverse conditions.展开更多
基金supported by the National Water Pollution Control and Management Technology Major Projects(No.2014ZX 07201-011)and(No.2015ZX 07202-013)the National Natural Science Foundation of China(No.51308010)the Research Fund for the Doctoral Program of Higher Education(No.20131103120017)
文摘The aim of the present work was to evaluate the anaerobic ammonium oxidation （anammox） activity of simultaneous partial nitrification, anammox and denitrification （SNAD） biofilm with different substrate concentrations and pH values. Kaldnes rings taken from the SNAD biofilm reactor were incubated in batch tests to determine the anammox activity. Haldane model was applied to investigate the ammonium inhibition on anammox process. As for nitrite inhibition, the NH4-N removal rate of anammox process remained 87.4% of the maximum rate with the NO2-N concentration of 100 mg/L. Based on the results of Haldane model, no obvious difference in kinetic coefficients was observed under high or low free ammonia （FA） conditions, indicating that ammonium rather than FA was the true inhibitor for anammox process of SNAD biofilm. With the pH value of 7.0, the rmax, KS and K of ammonium were 0.209 kg NO2-N/kg VSS/day, 9.5 mg/L and 422 rag/L, respectively. The suitable pH ranges for anammox process were B.0 to 9.0. These results indicate that the SNAD biofilm performs excellent tolerance to adverse conditions.
