Detection of the presence of Anastrepha striata and Anastrepha ludens in guava cultures is presently done in Mexico by actually catching the flies in strategically located traps. The objective of this study is to show...Detection of the presence of Anastrepha striata and Anastrepha ludens in guava cultures is presently done in Mexico by actually catching the flies in strategically located traps. The objective of this study is to show that it is possible to locate areas with the presence or absence of flies by remote sensing means, thereby simplifying the traditional way of detection of these plagues. In the field, groups of traps were selected where flies had been captured, as well as areas in which there was no capture in the traps. The radiometric signatures of whole trees and leaves were obtained, showing measurable differences between those specimens affected by the plague and those unaffected. Next we selected a SPOT5 image of 2007 corresponding to the study area in Calvillo, State of Aguascalientes, in Mexico, one of the major areas of guava cultures in the country. A supervised classification of the image allowed for the location of guava cultures in it. The guava culture areas obtained from this classification were validated comparing them to available maps of the cultured areas. Spectral signatures for the classes were derived from the image data. The separability of pairs of classes was also evaluated in order to maximize it. The IR/R (infrared to red ratio) ratio of the image bands was evaluated in 80 × 80 pixels around the locations of five traps where flies had been captured, and around five locations where the traps had not captured flies. Only the pixels where guava cultures were present were included in the analysis, other types of vegetation and soil coverage were rejected. We found that the index distributions with flies captured and those without flies captured cluster in two well-separated groups. We note that plotting the whole distribution of pixels around a trap yields a diagnostic view of the area, and individual index values do not provide such a view, since values with the flies' presence and without these overlap to some extent. Further analyses of other trap locations confirmed this separation and also revealed a third group of intermediate values between the two above, that are interpreted as locations in which the guava cultures are affected by the plague at an early stage of development, where the flies are not captured by the traps since they do not yet hatch. We concluded that it is possible to use remote sensing techniques to identify the presence ofAnastrepha striata and Anastrepha ludens in cultures of Psidium guajava L., even probably at early stages of development of the plague.展开更多
The behavioral responses of virgin and mated female Anastrepha striata Schiner (Diptera: Tephritidae) to guava (Psidium guajava L.) or sweet orange (Citrus sinensis L.) were evaluated separately using multilure...The behavioral responses of virgin and mated female Anastrepha striata Schiner (Diptera: Tephritidae) to guava (Psidium guajava L.) or sweet orange (Citrus sinensis L.) were evaluated separately using multilure traps in two-choice tests in field cages. The results showed that flies were more attracted to guava and sweet orange volatiles than to control (unbaited trap). The physiological state (virgin or mated) of females did not affect their attraction to the fruit volatiles. Combined analysis of gas chromatography coupled with electroantennography (GC-EAD) of volatile extracts of both fruits showed that 1 and 6 compounds from orange and guava, respectively elicited repeatable antennal responses from mated females. The EAD active compounds in guava volatile extracts were identified by gas chromatography-mass spectrometry (GC-MS) as ethyl butyrate, (Z)-3-hexenol, hexanol, ethyl hexanoate, hexyl acetate, and ethyl octanoate. Linalool was identified as the only antennal active compound in sweet orange extracts. In field cage tests, there were no significant differences between the number of mated flies captured by the traps baited with guava extracts and the number caught by traps baited with the 6-component blend that was formulated according to the relative proportions in the guava extracts. Similar results occurred when synthetic linalool was evaluated against orange extracts. From a practical point of view, the compounds identified in this study could be used for monitoring A. striata populations.展开更多
文摘Detection of the presence of Anastrepha striata and Anastrepha ludens in guava cultures is presently done in Mexico by actually catching the flies in strategically located traps. The objective of this study is to show that it is possible to locate areas with the presence or absence of flies by remote sensing means, thereby simplifying the traditional way of detection of these plagues. In the field, groups of traps were selected where flies had been captured, as well as areas in which there was no capture in the traps. The radiometric signatures of whole trees and leaves were obtained, showing measurable differences between those specimens affected by the plague and those unaffected. Next we selected a SPOT5 image of 2007 corresponding to the study area in Calvillo, State of Aguascalientes, in Mexico, one of the major areas of guava cultures in the country. A supervised classification of the image allowed for the location of guava cultures in it. The guava culture areas obtained from this classification were validated comparing them to available maps of the cultured areas. Spectral signatures for the classes were derived from the image data. The separability of pairs of classes was also evaluated in order to maximize it. The IR/R (infrared to red ratio) ratio of the image bands was evaluated in 80 × 80 pixels around the locations of five traps where flies had been captured, and around five locations where the traps had not captured flies. Only the pixels where guava cultures were present were included in the analysis, other types of vegetation and soil coverage were rejected. We found that the index distributions with flies captured and those without flies captured cluster in two well-separated groups. We note that plotting the whole distribution of pixels around a trap yields a diagnostic view of the area, and individual index values do not provide such a view, since values with the flies' presence and without these overlap to some extent. Further analyses of other trap locations confirmed this separation and also revealed a third group of intermediate values between the two above, that are interpreted as locations in which the guava cultures are affected by the plague at an early stage of development, where the flies are not captured by the traps since they do not yet hatch. We concluded that it is possible to use remote sensing techniques to identify the presence ofAnastrepha striata and Anastrepha ludens in cultures of Psidium guajava L., even probably at early stages of development of the plague.
文摘The behavioral responses of virgin and mated female Anastrepha striata Schiner (Diptera: Tephritidae) to guava (Psidium guajava L.) or sweet orange (Citrus sinensis L.) were evaluated separately using multilure traps in two-choice tests in field cages. The results showed that flies were more attracted to guava and sweet orange volatiles than to control (unbaited trap). The physiological state (virgin or mated) of females did not affect their attraction to the fruit volatiles. Combined analysis of gas chromatography coupled with electroantennography (GC-EAD) of volatile extracts of both fruits showed that 1 and 6 compounds from orange and guava, respectively elicited repeatable antennal responses from mated females. The EAD active compounds in guava volatile extracts were identified by gas chromatography-mass spectrometry (GC-MS) as ethyl butyrate, (Z)-3-hexenol, hexanol, ethyl hexanoate, hexyl acetate, and ethyl octanoate. Linalool was identified as the only antennal active compound in sweet orange extracts. In field cage tests, there were no significant differences between the number of mated flies captured by the traps baited with guava extracts and the number caught by traps baited with the 6-component blend that was formulated according to the relative proportions in the guava extracts. Similar results occurred when synthetic linalool was evaluated against orange extracts. From a practical point of view, the compounds identified in this study could be used for monitoring A. striata populations.