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Andrias davidianus bone peptides alleviates hyperuricemia-induced kidney damage in vitro and in vivo 被引量:1
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作者 Wei Li Haihong Chen +6 位作者 Hongliang Chen Ziyin Li Wei Hu Qinxuan Zhou Bing Xu Yi Wang Xinhui Xing 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第4期1886-1905,共20页
Hyperuricemia(HUA)is a vital risk factor for chronic kidney diseases(CKD)and development of functional foods capable of protecting CKD is of importance.This paper aimed to explore the amelioration effects and mechanis... Hyperuricemia(HUA)is a vital risk factor for chronic kidney diseases(CKD)and development of functional foods capable of protecting CKD is of importance.This paper aimed to explore the amelioration effects and mechanism of Andrias davidianus bone peptides(ADBP)on HUA-induced kidney damage.In the present study,we generated the standard ADBP which contained high hydrophobic amino acid and low molecular peptide contents.In vitro results found that ADBP protected uric acid(UA)-induced HK-2 cells from damage by modulating urate transporters and antioxidant defense.In vivo results indicated that ADBP effectively ameliorated renal injury in HUA-induced CKD mice,evidenced by a remarkable decrease in serum UA,creatinine and blood urea nitrogen,improving kidney UA excretion,antioxidant defense and histological kidney deterioration.Metabolomic analysis highlighted 14 metabolites that could be selected as potential biomarkers and attributed to the amelioration effects of ADBP on CKD mice kidney dysfunction.Intriguingly,ADBP restored the gut microbiome homeostasis in CKD mice,especially with respect to the elevated helpful microbial abundance,and the decreased harmful bacterial abundance.This study demonstrated that ADBP displayed great nephroprotective effects,and has great promise as a food or functional food ingredient for the prevention and treatment of HUA-induced CKD. 展开更多
关键词 andrias davidianus bone peptides HYPERURICEMIA Uric acid Chronic kidney disease Gut microbiota
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Nutritional and medicinal characteristics of Chinese giant salamander (Andrias davidianus) for applications in healthcare industry by artificial cultivation: A review 被引量:34
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作者 Dong He Wenming Zhu +8 位作者 Wen Zenga Jun Lin Yang Ji Yi Wang Chong Zhang Yuan Lu Daoquan Zhao Nan Su Xin-Hui Xing 《Food Science and Human Wellness》 SCIE 2018年第1期1-10,共10页
Andrias davidianus,i.e.Chinese giant salamander(CGS),is one of the largest and oldest amphibians existing in the world and is also one of the valuable biological resources of China.Wild CGS has been threatened with ex... Andrias davidianus,i.e.Chinese giant salamander(CGS),is one of the largest and oldest amphibians existing in the world and is also one of the valuable biological resources of China.Wild CGS has been threatened with extinction in the past decades due to over capturing,deterioration of natural environment,the slow breeding and growth of the wild species in nature.However,in the past twenty years,with the breakthrough and progress of artificial breeding technology by artificial insemination,the number of artificially cultivated CGS has increased rapidly.Artificially cultivated CGS can either be released to the CGS living environment to increase the population in nature or legally applied in food and medicinal industry as a feedstock due to the unique nutritional and medicinal values of CGS as recorded historically.In this review,the nutritional components,bioactive components and medicinal activities of the artificially cultivated CGS will be summarized.The mucus,skin,meat and bone of CGS contain many different bioactive substances thereby having various medicinal activities including anti-aging,anti-fatigue,anti-tumor,therapy of burn and anti-infection and other physiological functions.This paper will further discuss the potential applications of the artificially cultivated CGS in healthcare industry and prospects of future technological development. 展开更多
关键词 andrias davidianus Artificial breeding Chinese giant salamander Functional foods Medicinal activity Natural resource protection Nutrition
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Pathological Changes in Andrias davidianus Infected with Chinese Giant Salamander Ranavirus 被引量:2
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作者 Yi GENG Matthew James GRAY +6 位作者 Kaiyu WANG Defang CHEN Ping OUYANG Xiaoli HUANG Changliang HE Zhijun ZHONG Zexiao YANG 《Asian Herpetological Research》 SCIE CSCD 2016年第4期258-264,共7页
Chinese giant salamander ranavirus (CGSRV) is an emerging pathogen in captive populations of the Chinese giant salamander (Andrias davidianus). We processed 140 morbid Chinese giant salamanders from seven captive ... Chinese giant salamander ranavirus (CGSRV) is an emerging pathogen in captive populations of the Chinese giant salamander (Andrias davidianus). We processed 140 morbid Chinese giant salamanders from seven captive breeding populations over five years, and describe the disease associated with CGSRV infection. The most common gross signs were significant swelling of the legs and coelomic cavity, erythema of the legs and ventrum in juveniles; cutaneous erosions and ulcerations in adults, particularly the limbs and the head; and marked petechial or ecchymotic hemorrhages of the internal organs, particularly the liver, spleen and kidney. Histological examination showed degeneration, necrosis, and inflammation in many organs, particularly in the organs where hemorrhage was observed. There was evidence of eosinophilic inclusion bodies in degenerated and necrotic cells. We identified virus particles and empty capsids without viral nucleoid in the inclusion bodies using electron microscopy. Virus particles were hexagonal or round shape, and appeared in paracrystalline arrays, aggregates, or singly. All enveloped viral particles were 140-160 nm. Polymerase chain reaction followed by sequencing verified that the virus particles were CGSRV. These results collectively support that CGSRV was the etiologic agent responsible for these mass die-offs of the Chinese giant salamander. The pathology described herein will be useful in diagnosing cases of ranaviral disease caused by CGSRV, and provide evidence that this pathogen is a significant threat to the Chinese giant salamander. 展开更多
关键词 PATHOLOGY CGSRV andrias davidianus RANAVIRUS China
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Effects of Water Quality on the Distribution of Chinese Giant Salamander(Andrias davidianus)in Guizhou Province,China 被引量:1
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作者 Xue LI Jingcai LV +2 位作者 Shize LI Ning XU Gang WEI 《Agricultural Science & Technology》 CAS 2017年第8期1500-1504,共5页
13 water quality parameters were tested from 38 reaches of 34 counties in Guizhou where Chinese giant salamander(Andrias davidianus) distributed over the past 30 years. Dissolved oxygen and p H were found to be signif... 13 water quality parameters were tested from 38 reaches of 34 counties in Guizhou where Chinese giant salamander(Andrias davidianus) distributed over the past 30 years. Dissolved oxygen and p H were found to be significant determinants of the species distribution in recent years(P<0.05). There was no Chinese giant salamander distribution in the recent five years in rivers with dissolved solids of greater than 415 mg/L, conductivity of greater than 639 us/cm, salinity of greater than 0.31 ppt and total hardness of greater than 150 mg/L. Sensitive to environment, the Chinese giant salamander is an important indicator for environmental quality, so it could be used as one of the environmental indicator. Eutrophication, chemical fertilizer, pesticides and inorganic pollutants may be one of reasons driving wild CGS into extinction. 展开更多
关键词 Chinese giant salamander andrias davidianus) Guizhou province Water quality factors
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Isolation and Properties of Collagen from the Skin of Andrias davidianus
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作者 Li Hua Deng Lin 《Animal Husbandry and Feed Science》 CAS 2014年第6期319-321,324,共4页
[ Objective] To explore and utilize the collagen from the skin of bred Andrias davidianus. [ Method] Collagen was isolated with enzymolysis technology at 4℃, and the compositions and some biochemical properties were ... [ Objective] To explore and utilize the collagen from the skin of bred Andrias davidianus. [ Method] Collagen was isolated with enzymolysis technology at 4℃, and the compositions and some biochemical properties were studied. [ Result] The content of the hydroxyproline in the skin ofA. davidianus was 22.3 %, the collagen extraction ratio was 26.7% ( based on wet weight), the contents of the moisture, crude ash, crude fat, and crude protein were 6.75%, 0.62%, 0.38% and 92.10%, respectively. The collagen contained 8 essential amino acids for adult and 2 essential amino acids for children, the contents of the imlno acid was 171 residues/1 000 residues, and hydroxylation of proline was 51.5%. As ultraviolet (UV) absorption spectrum showed, the strong absorption peak was near 230 nm, according to the SDS - PAGE, it consisted of two diffcremt α chains ( αl and α2) which was characterized to be type I collagen. [ Conclusion] Extraction of collagen from the skin of bred A. davidianus is feasible theoretically, the extraction rate and purity arc very high. 展开更多
关键词 andrias davidianus SKIN COLLAGEN ISOLATION PROPERTY
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Collected Data of Local Ecological Knowledge to Predict Current Status and Potential Threats of salamander(Andrias davidianusin)in Zhengan County of Guizhou Province,China
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作者 Zijing SU Jingcai LYU +1 位作者 Shize LI Gang WEI 《Agricultural Science & Technology》 CAS 2017年第8期1487-1491,1496,共6页
Chinese giant salamander(CGS, Andrias davidianus) is experiencing the threat of extinction. It is disappearing gradually, because of overharvesting, environmental pollution, specially river pollution, activity of huma... Chinese giant salamander(CGS, Andrias davidianus) is experiencing the threat of extinction. It is disappearing gradually, because of overharvesting, environmental pollution, specially river pollution, activity of human exploitation, and so on. We conducted community interview fieldworks to collect respondent data from Xieba Country(not reserve) situated in Zhengan County of Guizhou Province, where one of the places known by historical giant salamander records in Guizhou. Through the analysis of the collected data, we created quantitative data of use for conservation management on the local status of salamander populations, and we compared our data with those available data of Y. Pan et al., who investigated the status of CGS in 3 reserves in Guizhou Province in 2015.The conclusions have a similar trend, which proved that the collected data were creditable. Through statistical analysis of the comparison data, the number of wild CGS decreased actually followed the increase of artificial framing.Because of the source of breeding to catch wild CGS, overharvesting was likely the most reason to make wild CGS decline. 展开更多
关键词 Local ecological knowledge (LEK) Chinese giant salamander andrias davidianus Zhengan Community interview QUESTIONNAIRE
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Bioinspired Andrias davidianus-Derived wound dressings for localized drug-elution 被引量:2
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作者 Xiang Liu Xiang Mao +9 位作者 Guo Ye Menghong Wang Ke Xue Yan Zhang Hongmei Zhang Xiaoqiao Ning Man Zhao Jinlin Song Yu Shrike Zhang Ximu Zhang 《Bioactive Materials》 SCIE 2022年第9期482-494,共13页
Local drug delivery has received increasing attention in recent years.However,the therapeutic efficacy of local delivery of drugs is still limited under certain scenarios,such as in the oral cavity or in wound beds af... Local drug delivery has received increasing attention in recent years.However,the therapeutic efficacy of local delivery of drugs is still limited under certain scenarios,such as in the oral cavity or in wound beds after resection of tumors.In this study,we introduce a bioinspired adhesive hydrogel derived from the skin secretions of Andrias davidianus(SSAD)as a wound dressing for localized drug elution.The hydrogel was loaded with aminoguanidine or doxorubicin,and its controlled drug release and healing-promoting properties were verified in a diabetic rat palatal mucosal defect model and a C57BL/6 mouse melanoma-bearing model,respectively.The results showed that SSAD hydrogels with different pore sizes could release drugs in a controllable manner and accelerate wound healing.Transcriptome analyses of the palatal mucosa suggested that SSAD could significantly upregulate pathways linked to cell adhesion and extracellular matrix deposition and had the ability to recruit keratinocyte stem cells to defect sites.Taken together,these findings indicate that property-controllable SSAD hydrogels could be a promising biofunctional wound dressing for local drug delivery and promotion of wound healing. 展开更多
关键词 andrias davidianus Hydrogel Drug loading Controlled drug delivery Stem cell recruitment Wound healing
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The Expresion of Quox-1 Gene in the Embryo Development of Andrias davianus
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作者 Deng Fengjiao Feng Xiaorong +5 位作者 Zheng Junying Liu Ting Yi Qingming Zhang Xiyuan Xiao Hanbing Xue Zhigang 《Wuhan University Journal of Natural Sciences》 CAS 1997年第2期104-107,共4页
Two set of Andrias davidanus fertilized eggs and its seedlings were got by means of artificial re production.The sections of the embryo of Andrias davidianus were made by histological method The expres sion of homolog... Two set of Andrias davidanus fertilized eggs and its seedlings were got by means of artificial re production.The sections of the embryo of Andrias davidianus were made by histological method The expres sion of homologous sequences of Quox 1 gene has been studied by immunohistochemical method with special antibody.Results show that the expression of homologous sequences of Quox 1 gene was detected in the epi dermis of blastula and gastula.The highly expression was investigated in the epidermis at the neural tube phrase and the tail bud stage. 展开更多
关键词 Quox-1 gene andrias davidianus embryo development immunohistochemical method
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Protection and Utilization of Chinese Giant Salamander Germplasm Resources
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作者 Jinxing GU Guoxi LI +3 位作者 Zhenjiang YANG Bianzhi LIU Huihui WU Daoquan ZHAO 《Asian Agricultural Research》 2023年第2期35-38,共4页
Chinese giant salamander(Andrias davidianus)is salamander germplasm resource that only distributed in China.It is the material basis of the evolution principle research,genetic research and artificial breeding.Since t... Chinese giant salamander(Andrias davidianus)is salamander germplasm resource that only distributed in China.It is the material basis of the evolution principle research,genetic research and artificial breeding.Since the 1990s,overharvesting,water pollution and habitat changes have seriously destroyed the germplasm resources of A.davidianus,their population density has decreased since then.Strengthening the protection and rational utilization of A.davidianus germplasm resources are key factors in impacting the healthy,stable and sustainable development of A.davidianus industry.Under the above background,we discussed the current problems with the protection for A.davidianus germplasm resources,put forward suggestions for the conservation of A.davidianus germplasm resources.It is expected to provide a reference to effective protection for A.davidianus germplasm resources,and provide a scientific foundation for further studies,so as to promote the high-quality development of related industry. 展开更多
关键词 Chinese giant salamander(andrias davidianus) Germplasm resources Protection Utilization
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旅游干扰对张家界市大鲵饵料鱼及大鲵种群数量的影响 被引量:1
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作者 罗庆华 陶水秀 +5 位作者 蒋万胜 胡骁 刘柯 陈进豪 付磊 曹威 《生命科学研究》 CAS CSCD 2020年第3期199-207,共9页
为了探究旅游干扰对国家Ⅱ级保护动物--中国大鲵(Andrias davidianus)种群数量及其饵料鱼种类、资源量的影响,对湖南省张家界市重度与轻度旅游干扰下大鲵栖息地的旅游干扰内容、饵料鱼的种类与渔获量、大鲵种群数量进行了同步调查与估算... 为了探究旅游干扰对国家Ⅱ级保护动物--中国大鲵(Andrias davidianus)种群数量及其饵料鱼种类、资源量的影响,对湖南省张家界市重度与轻度旅游干扰下大鲵栖息地的旅游干扰内容、饵料鱼的种类与渔获量、大鲵种群数量进行了同步调查与估算,并比较了旅游重度干扰区在旅游开发前后饵料鱼种类的差异,以及旅游重度与轻度干扰区饵料鱼种类与渔获量的差异。结果表明:在湖南省张家界市的大鲵栖息地共发现饵料鱼类4目10科22属25种;旅游重度干扰区现在饵料鱼的物种数大于旅游开发前,也大于轻度干扰区,但统计上无显著差别;Jaccard系数分析显示重度与轻度干扰区之间饵料鱼物种组成的相似性较小;与轻度干扰区相比,重度干扰区的渔获量较多,但大鲵种群数量较少。以上信息提示,在张家界市目前适度开发和管理模式下,旅游干扰对大鲵饵料鱼资源没有产生明显的负面影响,但重度干扰区可能由于游客活动带来的噪声增加或者洞穴数的减少等其他干扰因素使大鲵种群数量有所下降。 展开更多
关键词 旅游干扰 饵料鱼 栖息地 中国大鲵(andrias davidianus) 张家界
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大鲵MC1R基因的克隆、序列分析与表达研究
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作者 邓捷 卫小燕 +5 位作者 张晗 马红英 王启军 赵虎 张红星 姜维 《河北渔业》 2020年第10期24-29,34,共7页
为研究MC1R在两栖动物体色形成中的作用,试验结合转录组分析和电子克隆技术得到大鲵(Andrias davidianus)MC1R部分cDNA序列并进行了生物信息学分析,采用实时荧光定量法检测了大鲵MC1R基因在皮肤、肝脏等10个组织中的表达情况及4种不同... 为研究MC1R在两栖动物体色形成中的作用,试验结合转录组分析和电子克隆技术得到大鲵(Andrias davidianus)MC1R部分cDNA序列并进行了生物信息学分析,采用实时荧光定量法检测了大鲵MC1R基因在皮肤、肝脏等10个组织中的表达情况及4种不同体色大鲵皮肤组织中MC1R基因的表达量。结果表明:得到大鲵MC1R部分cDNA长为295 bp,位于该基因编码区。其与人、小鼠等哺乳动物和鸟类MC1R核苷酸同源性在57%~83%。大鲵MC1R基因与粗皮渍螈的亲缘关系最近。该基因在不同组织中均有表达,以皮肤组织表达量最高。4种不同体色大鲵皮肤组织中,黑色皮肤中MC1R基因表达量高于其他体色。说明大鲵MC1R基因在体色形成过程中可能具有与其他两栖动物不同的调控机制。 展开更多
关键词 大鲵(andrias davidianus) 体色 黑色素 黑皮质素受体-1(Melanocortin-1 receptor MC1R)基因 表达
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大鲵虹彩病毒病灭活疫苗安全性与免疫效力研究 被引量:2
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作者 索钰杰 林格 +7 位作者 江南 李逸群 范玉顶 刘文枝 孟彦 薛明洋 曾令兵 周勇 《淡水渔业》 CSCD 北大核心 2021年第4期34-41,共8页
为了探明大鲵虹彩病毒病灭活疫苗的安全性与免疫效力,采用β-丙内酯(β-propiolactone,BPL)灭活鲤上皮瘤细胞(Epithelioma papilloma cyprini,EPC)内增殖的大鲵虹彩病毒(giant salamander iridovirus,GSIV),制备成大鲵虹彩病毒病灭活疫... 为了探明大鲵虹彩病毒病灭活疫苗的安全性与免疫效力,采用β-丙内酯(β-propiolactone,BPL)灭活鲤上皮瘤细胞(Epithelioma papilloma cyprini,EPC)内增殖的大鲵虹彩病毒(giant salamander iridovirus,GSIV),制备成大鲵虹彩病毒病灭活疫苗,利用免疫剂量为1.0×10^(6) TCID_(50)/mL的灭活疫苗腹腔注射健康大鲵(Andrias davidianus),研究疫苗的安全性和免疫效力。结果显示:一次单剂量注射、单剂量重复注射和超剂量注射后,大鲵的行为和摄食均正常,未见不良反应,证明了制备的大鲵虹彩病毒病灭活疫苗安全;用不同剂量的疫苗(0.1、0.2、0.3、0.4、0.5 mL/尾)免疫健康大鲵,在免疫后第28 d用GSIV进行攻毒,当免疫剂量为0.1 mL/尾时,相对免疫保护率为44.44%,其它4种免疫剂量的相对免疫保护率相似,均高于70%。大鲵在免疫后7 d已开始产生免疫应答,21 d血清抗体效价达到最高值252.48±28.01。在免疫后两周内,MyD88基因、TLR9基因和MHCIIA基因均显著上调。免疫后150 d进行攻毒实验,大鲵的相对免疫保护率为62.5%。二次免疫实验中,大鲵分别在第一次免疫7、14、21、28、56 d后进行第二次免疫。结果显示,第一次免疫21 d后进行二次免疫,其血清抗体效价可以较长时间维持在最高水平,最高血清抗体效价为274.37±25.23,第二次免疫后的第150 d用GSIV进行攻毒,存活率为86.67%。研究结果表明,大鲵虹彩病毒病灭活疫苗最佳免疫方式为腹腔注射疫苗0.2 mL/尾后,21 d进行第二次免疫,免疫剂量为0.2 mL/尾。 展开更多
关键词 大鲵(andrias davidianus) 虹彩病毒 灭活疫苗 安全性 免疫效力
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大鲵寄生虫性疾病研究进展 被引量:1
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作者 张晗 邓捷 +5 位作者 赵虎 马红英 王启军 车万宽 张红星 孔飞 《河北渔业》 2021年第2期37-38,46,共3页
中国大鲵(Andrias davidianus)寄生虫性疾病严重影响大鲵养殖产业的可持续发展,本文针对已知的大鲵寄生虫性疾病的致病病原体、疾病症状及防治措施进行了概述。
关键词 中国大鲵(andrias davidianus) 寄生虫性疾病 防治
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一株大鲵虹彩病毒的分离及鉴定
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作者 喻大鹏 程俊 +3 位作者 夏洪丽 夏立群 蔡佳 鲁义善 《淡水渔业》 CSCD 北大核心 2022年第4期11-17,共7页
2020年5月,广东广州某野生动物保护基地爆发疑似病毒引发的疾病。现场采样发现,病鲵体长约2 m,反应迟钝,体表有出血点或溃疡症状。本研究采用胖头鲤肌肉细胞系(fat head minnow epithelial cells,FHM)培养、超薄切片透射电镜观察、病毒... 2020年5月,广东广州某野生动物保护基地爆发疑似病毒引发的疾病。现场采样发现,病鲵体长约2 m,反应迟钝,体表有出血点或溃疡症状。本研究采用胖头鲤肌肉细胞系(fat head minnow epithelial cells,FHM)培养、超薄切片透射电镜观察、病毒主要衣壳蛋白(major capsid protein,MCP)克隆与测序分析等方法,从患病大鲵中分离得到一株病毒,鉴定其属于虹彩病毒科蛙病毒属,命名为大鲵虹彩病毒广州分离株(CGSIV-GZ)。FHM经患病大鲵组织匀浆液接种后出现细胞圆缩、死亡、脱落等典型的细胞病变症状。将感染后的FHM细胞制作超薄切片,通过电镜观察发现,FHM细胞中存在大量直径约100~120 nm具囊膜的正六边形成熟病毒粒子,形态与虹彩病毒相似。根据虹彩病毒MCP基因保守区域序列设计特异性引物对病鲵组织样本进行PCR扩增,获得了431 bp的目的基因片段,测序后经采用BLAST软件分析,发现其与GenBank中的蛙病毒衣壳蛋白基因同源性达96%~99%。确认本次野生动物保护基地大鲵发生大规模死亡是蛙病毒感染所致。 展开更多
关键词 大鲵(andrias davidianus) 虹彩病毒科 透射电镜 胖头鲤肌肉细胞系 分离鉴定
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Genetic Bottlenecks of the Wild Chinese Giant Salamander in Karst Caves 被引量:5
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作者 Jie WANG Hongxing ZHANG +2 位作者 Feng XIE Gang WEI Jianping JIANG 《Asian Herpetological Research》 SCIE CSCD 2017年第3期174-183,共10页
The rarity of limestone cave species due to habitat degradation makes them of special interest in conservation biology.The wild Chinese Giant Salamander Andrias davidianus,an evolutionarily distinct and globally endan... The rarity of limestone cave species due to habitat degradation makes them of special interest in conservation biology.The wild Chinese Giant Salamander Andrias davidianus,an evolutionarily distinct and globally endangered species,are nearly all obligate into living in inaccessible mountain caves now.We detected that only 14-29 breeders,with the effective population size of 9-25(5-44 of 95% confidence interval),were in each of three caves,through genotyping 20 microsatellite loci on larvae that were flushed out of caves.Both heterzygosity excess and M(ratios of allele number to allele size range) tests indicated severe genetic bottlenecks among populations.Both mitochondrial,with only one or two haplotypes of D-loop region(770-771bp) in each population,and nuclear genetic structure showed clear divergence between populations.Considering the long life history,small population size,and genetic differentiationof the Chinese Giant Salamander,putting an end to poaching and recovering the karst ecosystem instead of releasing may be the sole measures to save this severely threated species. 展开更多
关键词 limestone cave small population genetic drift andrias davidianus subterranean habitat
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洛阳伊水大鲵肌肉营养成分分析 被引量:3
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作者 李斌 闫润堃 +3 位作者 普畅畅 刘晓林 王钰鑫 黄勇 《河北渔业》 2021年第2期12-17,共6页
采用常规营养成分分析方法测定了洛阳伊水大鲵(Andrias davidianus)肌肉的粗蛋白质、粗脂肪、氨基酸、脂肪酸、矿物质和微量元素等营养成分。大鲵肌肉中粗蛋白含量为(15.68±0.58)%,粗脂肪含量为(2.43±0.03)%,水分含量为(76.93... 采用常规营养成分分析方法测定了洛阳伊水大鲵(Andrias davidianus)肌肉的粗蛋白质、粗脂肪、氨基酸、脂肪酸、矿物质和微量元素等营养成分。大鲵肌肉中粗蛋白含量为(15.68±0.58)%,粗脂肪含量为(2.43±0.03)%,水分含量为(76.93±2.64)%和粗灰分含量(0.72±0.05)%。肌肉中共检测到18种氨基酸,其中8种人体必需氨基酸总量为6.01%,占氨基酸总量的41.08%;4种鲜味氨基酸总量为6.77%,占氨基酸总量的46.27%。肌肉中不饱和脂肪酸含量高达74.45%,富含EPA和DHA,较一般水生经济动物高。此外大鲵肌肉中含有丰富的矿物质元素,其中钾含量最高,达到了(2800±20.40)mg/kg;微量元素中,锌和铁含量较高,硒也达到了富硒标准,含量为(0.42±0.04)mg/kg,表明洛阳伊水大鲵肌肉具有较高的营养价值,能为人类提供优质蛋白。 展开更多
关键词 伊水大鲵(andrias davidianus) 肌肉 氨基酸 脂肪酸 矿物质 微量元素
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黔南地区成体大鲵生长及生活习性的周年观察
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作者 陈嶙 熊洪林 +2 位作者 玉志文 文狄 黄小娜 《湖北农业科学》 2020年第14期133-136,共4页
对黔南地区成体大鲵(Andrias davidianus)进行了14个月喂养观察试验。结果表明,成体大鲵体长生长和体质量生长是两个完全不同的过程,二者之间反向关系明显;体长生长与水温呈正向关系;体质量生长与水温关系复杂,有高温和低温限制。大鲵... 对黔南地区成体大鲵(Andrias davidianus)进行了14个月喂养观察试验。结果表明,成体大鲵体长生长和体质量生长是两个完全不同的过程,二者之间反向关系明显;体长生长与水温呈正向关系;体质量生长与水温关系复杂,有高温和低温限制。大鲵喜欢阴暗安静的环境,食性范围较广;排出团状或细沙状粪便,且进食越多,排便次数就越多。大鲵具有蜕皮习性,蜕皮次数与体质量生长具有正向关系。黔南地区饲养的大鲵很少冬眠,在生长高峰期投喂充足饵料,能够有效促进增重率;用福寿螺配合饲料喂养鲤鱼,再用活鲤鱼喂养大鲵,可以显著提高人工养殖大鲵的经济效益。 展开更多
关键词 大鲵(andrias davidianus) 生长 生活习性 福寿螺
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Efficient scarless skin regeneration enabled by loading micronized amnion in a bioinspired adhesive wound dressing
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作者 Huicong Zhang Mingyu Song +11 位作者 Changhong Hu Zhiyi Zhang Shuqi Zhang Yan Zhang Yingming Yang Puxu Zhou Liwen Zheng Lan Li Mengjie Mao Yu Shrike Zhang Ping Ji Ximu Zhang 《Aggregate》 2023年第5期96-111,共16页
Complete skin reconstruction is a hierarchical,physiological assembly process involving healing of the epidermis,dermis,vasculature,nerves,and cutaneous appendages.To date,few works have reported complete skin regener... Complete skin reconstruction is a hierarchical,physiological assembly process involving healing of the epidermis,dermis,vasculature,nerves,and cutaneous appendages.To date,few works have reported complete skin regeneration,particularly lacking vascular structures and hair follicles after full skin defects.In this study,a hydrogel derived from the skin secretion of Andrias davidianus(SSAD)that features adhesiveness was used as a bioactive scaffold to load micronized amnion(MA).The SSAD hydrogel was found to promote the migration and proliferation of amnion stem cells and human keratinocytes,as well as inhibit their apoptosis in vitro.In a rat full-skin defect model,the regeneration of skin appendages was observed at the wound area,achieving scarless healing.Transcriptome analyses further validated that SSAD could positively regulate cell migration,proliferation,and differentiation.These functions might be attributed to the abundant growth factors present in the SSAD.Synergized by the delivery of MA,SSAD loaded with the MA could achieve a significantly better skin regeneration effect than SSAD or MA used alone,providing a simple yet highly effective means to obtain complete,scarless skin regeneration,suggesting favorable potential for clinical translation. 展开更多
关键词 AMNION andrias davidianus cell delivery cutaneous appendages SCARLESS wound dressing
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Development of an eDNA metabarcoding tool for surveying the world’s largest amphibian 被引量:2
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作者 Jie Wang Ping Liu +3 位作者 Jiang Chang Cheng Li Feng Xie Jianping Jiang 《Current Zoology》 SCIE CAS CSCD 2022年第5期608-614,共7页
Due to the overexploitation of farming,as well as habitat destruction,the wild population of Chinese giant salamander(CGS)Andrias davidianus,a species with seven genetically distinct lineages,has decreased by over 80%... Due to the overexploitation of farming,as well as habitat destruction,the wild population of Chinese giant salamander(CGS)Andrias davidianus,a species with seven genetically distinct lineages,has decreased by over 80%in the past 70 years.Traditional survey methods have proven to be unsuitable for finding this rare and elusive species.We evaluated the efficacy of environmental DNA(eDNA)sampling to detect CGS indirectly from its aquatic environment.We developed several species-specific primer sets;validated their specificity and sensitivity;and assessed their utility in silico,in the laboratory,and at two field sites harboring released farm-bred CGS.We detected the presence of CGS DNA by using polymerase chain reaction and Sanger sequencing.We also sequenced an amplicon mixture of seven haplotype-represented samples using high-throughput sequencing.Our eDNA methods could detect the presence of CGS at moderate densities reported across its range,proving them as a cost-effective way to establish broad-scale patterns of occupancy for CGS.In addition,our primers enabled the detection of mitochondrial lineage mixture or introduced individuals from geographically isolated populations of CGS. 展开更多
关键词 andrias davidianus Chinese giant salamander EDNA metabarcoding population survey
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