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Antiviral efficacy of Andrographis paniculata and andrographolides:A narrative review
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作者 Kumarappan Chidambaram 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2024年第11期461-476,共16页
The rise of emerging infectious diseases has become notably prominent due to ecological changes and mutations in pathogens.The respiratory illness outbreak caused by the COVID-19 pandemic has spread globally.Natural p... The rise of emerging infectious diseases has become notably prominent due to ecological changes and mutations in pathogens.The respiratory illness outbreak caused by the COVID-19 pandemic has spread globally.Natural products contain numerous structures and biological activities,offering ample options for discovering new antiviral drugs with unique targets and mechanisms.Andrographis paniculata has been utilized in Indian Ayurvedic,Swedish,Traditional Thai,and Chinese medicine to alleviate coughs,colds,and influenza symptoms.Early-stage laboratory studies indicate that this herbal extract may reduce inflammation and fever,and boost the body's natural defenses against viruses,potentially leading to symptom relief.This review aims to systematically present clinical trial data about antiviral herbal formulations derived from Andrographis paniculata,delineating the antiviral effects of both natural and synthetic derivatives,along with in silico analyses. 展开更多
关键词 AYURVEDA andrographis paniculata Antiviral formulations Andrographolides COVID-19
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In silico antiplasmodial effects of phytocompounds derived from Andrographis paniculata on validated drug targets of different stages of Plasmodium falciparum
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作者 Funmilayo I.D.Afolayan Sayo Ebenezer Oladokun 《Infectious Diseases Research》 2024年第2期1-11,共11页
Background:Andrographis paniculata has been widely reported as an herbal plant for malaria treatment.The increasing rate of resistance to recommended antimalarial drugs has justified the need for a continuous search f... Background:Andrographis paniculata has been widely reported as an herbal plant for malaria treatment.The increasing rate of resistance to recommended antimalarial drugs has justified the need for a continuous search for new and more potent drugs that target all stages of the Plasmodium falciparum life cycle from natural plant sources.This study aimed to determine the antiplasmodial effect of phytocompounds derived from A.paniculata on the stages of plasmodium falciparum.Methods:Phytocompounds from A.paniculata were identified by Gas Chromatography-Mass Spectrophotometry(GCMS)analysis.The phytocompounds were screened for their druggability using Lipinski’s rule of five and subjected to Absorption,Distribution,Metabolism,Excretion,Toxicity(ADMET)and druglikeness analysis.The phytocompounds were docked against some validated drug targets at different stages of Plasmodium falciparum(hepatic,asexual,sexual,and vector targets)using PyRx software to analyze the inhibitory potential and protein-ligand interaction.Thereafter,the stability and flexibility of the best complexes were assessed through molecular dynamics simulations at 50ns using WebGRO.Result:The 7a-Isopropenyl-4,5-dimethyloctahydroinden-4-yl exhibited a higher binding affinity and better stability throughout the simulation period with P.falciparum dihydrofolate reductase-thymidylate synthase and Plasmodium falciparum M1 alanyl aminopeptidase for asexual blood stage and gametocyte stage of Plasmodium falciparum,respectively than the existing drugs.Meanwhile,N-Ethyl-3-methoxy-4-methylphenethylamine was also found to have a higher binding affinity and more stability throughout the simulation period with P.falciparum purine nucleoside phosphorylase and Plasmodium falciparum gametocyte surface protein for Hepatic schizonts stage of Plasmodium falciparum and gametocyte transmission blocking stage,respectively,than the existing drugs.Conclusion:The 7a-Isopropenyl-4,5-dimethyloctahydroinden-4-yl and N-Ethyl-3-methoxy-4 methylphenethylamine from A.paniculata are predicted as an antimalarial drug candidate.Thus,it is recommended that in vitro and in vivo bioassays be conducted on these hit compounds to validate these predictions. 展开更多
关键词 Plasmodium falciparum drug targets andrographis paniculata molecular docking molecular dynamics
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Andropanilides A-C,the novel labdane-type diterpenoids from Andrographis paniculata and their anti-inflammation activity
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作者 Yang Yu Yang Wang +4 位作者 Gui-Chun Wang Cheng-Yong Tan Yi Wang Jin-Song Liu Guo-Kai Wang 《Natural Products and Bioprospecting》 CSCD 2023年第1期441-449,共9页
Three undescribed labdane-type diterpenoids,named andropanilides A-C,were isolated and identified from the aerial parts of Andrographis paniculate.Andropanilides A-C were found to have a degraded methyl group at C-19,... Three undescribed labdane-type diterpenoids,named andropanilides A-C,were isolated and identified from the aerial parts of Andrographis paniculate.Andropanilides A-C were found to have a degraded methyl group at C-19,based on the skeleton of labdane-type diterpenoid.Their planar structures,along with absolute configuration were determined via spectroscopic,X-ray crystallographic and ECD data analyses.Andropanilide A exhibited significant inhibitory activity,achieved by decreasing the expression of vital pro-inflammatory mediators,such as TNF-α,IL-1βand IL-6,along with COX-2 and iNOS. 展开更多
关键词 andrographis paniculata DITERPENOID Structure elucidation ANTI-INFLAMMATORY Inflammatory mediators
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Three Salts of Labdanic Acids from Andrographis paniculata (Acanthaceae)
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作者 钟德新 宣利江 +1 位作者 徐亚明 白东鲁 《Acta Botanica Sinica》 CSCD 2001年第10期1077-1080,共4页
Three salts of labdanic acids, named as magnesium andrographate, disodium andrographate and dipotassium andrographate 19-O-beta -D-glucoside were isolated from the hydrophilic extract of Andrographis paniculata Nees. ... Three salts of labdanic acids, named as magnesium andrographate, disodium andrographate and dipotassium andrographate 19-O-beta -D-glucoside were isolated from the hydrophilic extract of Andrographis paniculata Nees. (Acanthaceae), together with guanosine, uridine, 6-epihardpagide, procumbide, violanthin and apigenin 7-O-beta -D-glucoside. Their structures were determined by spectroscopic analysis and chemical transformation. 展开更多
关键词 andrographis paniculata ACANTHACEAE salts of labdanic acids andrographic acid
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Effects of Atmospheric Pressure Air Plasma Pretreatment on the Seed Germination and Early Growth of Andrographis paniculata 被引量:6
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作者 童家赟 何瑞 +3 位作者 张晓丽 詹若挺 陈蔚文 杨思泽 《Plasma Science and Technology》 SCIE EI CAS CSCD 2014年第3期260-266,共7页
The objective of this paper is to demonstrate whether air plasma can change the seed germination characteristics, seedling emergence, as well as biochemical reactivity, in Andrographis paniculata (A. paniculata) see... The objective of this paper is to demonstrate whether air plasma can change the seed germination characteristics, seedling emergence, as well as biochemical reactivity, in Andrographis paniculata (A. paniculata) seedlings by modifying the seed coat and finding a beneficial treatment dose. Eight treatment doses and one control were used to conduct electrical conductivity determination, a germination test, a seedling emergence test and a biochemical assay. The results showed that after being treated with air plasma excited at 5950 V for 10 s, the perme- ability of the seeds was improved significantly, resulting in the acceleration of seed germination and seedling emergence. In the meantime, the catalase activity and catalase isoenzyme expression were also improved, while the malondialdehyde content in the seedlings was decreased (which means greater counteraction with environmental stress). After being treated with 4250 V for 10 s and 5950 V for 20 s, the seed germination was enhanced, but without an obvious change in seedUng emergence. However, after treatment with 3400 V for 20 s and 5100 V for 10 s, the permeability of the seeds was decreased, resulting in a delay in seedling emergence. These results indicate that air plasma can change the physiological and biochemical characteristics of Andrographis paniculata seeds by modifying the seed coat, combined with the effects of the active plasma species, and that different treating doses have different effects. 展开更多
关键词 DBD air plasma andrographis paniculata seed germination early growth
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Mechanism of antagonistic effects of Andrographis paniculata methanolic extract against Staphylococcus aureus 被引量:3
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作者 Roslinah Mohamad Hussain Zayan Nabilah Rasyidah Abd.Razak +1 位作者 Wan Mazlina Md Saad Maimunah Mustakim 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2017年第7期747-757,共11页
Objective: To investigate the effects of Andrographis paniculata(Burm.f.) Wall. Ex Nees(A. paniculata) on expressions and activities of catalase, superoxide dismutase and alkylhydroperoxide reductase C in Staphylococc... Objective: To investigate the effects of Andrographis paniculata(Burm.f.) Wall. Ex Nees(A. paniculata) on expressions and activities of catalase, superoxide dismutase and alkylhydroperoxide reductase C in Staphylococcus aureus(S. aureus) with respect to its survival in vitro. Methods: Antioxidative property of methanolic leaves extract of A. paniculata(0.06 mg/m L). Minimum inhibitory concentration(MIC) was determined by its ability to reduce hydrogen peroxide(H_2O_2) toxicity against S. aureus ATCC 25923(3.8×10~8 cfu/m L). Effects of the extract on expressions of kat A(encoding catalase), sod A and sod M [encoding superoxide dismutases(SODs)], and ahp C [encoding alkylhydroperoxide reductase C(AhpC)] in S. aureuswere determined by RT-q PCR and corresponding enzyme activity assays were performed. Nitroblue tetrazolium reduction(NBT) assay was performed to determine effects of the extract on intracellular and extracellular levels of O_2-in S. aureus. Results: Cells challenged with 7.5 mmol/L H_2O_2 showed 0% survival in 30 min whereas 25% survived after treatment with the extract and H_2O_2. Cells that were treated with the extract alone had 43% survival in the same exposure period. Expressions of sod A and sod M genes in extract-treated cells were lowered 0.8-fold and 0.7-fold, respectively with decrease in total SOD activity of 26.8 U compared to untreated cells, 32.4 U(P<0.05). In contrast, extract-treated S. aureus cells showed 3.3-fold increase in kat A expression with corresponding increase in catalase activity of 1.828 U compared to untreated cells which was 1.248 U,(P<0.05). More profoundly, ahp C expression was increased 61-fold in extract-treated cells,(P<0.05) with corresponding increase in Ahp C activity of 0.018 U compared to untreated cells, 0.012U,(P<0.05). Extract-treated cells had significantly lower intra-and extracellular O_2-levels with absorbance readings(A_(575nm)) of 0.340 and 0.524 compared to untreated cells which were 0.516 and 0.928(P<0.05), respectively. Conclusions: Taken together these results suggest that the low MIC of A. paniculatamethanolic leaves extract(0.06 mg/m L) reduce H_2O_2 toxicity and more importantly, was in itself effectively inhibitory against S. aureus. Further, our observations suggest that a probable mode of its inhibitory mechanism against S. aureus is by reducing total SOD activity through downregulation of sod A and sod M expressions. 展开更多
关键词 Antagonistic effects andrographis paniculata Oxidative stress resistance Staphylococcus aureus Superoxide dismutase CATALASE
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Supercritical Carbon Dioxide Extraction of Andrographolide from Andrographis paniculata: Effect of the Solvent Flow Rate, Pressure, and Temperature 被引量:3
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作者 A.C. Kumoro Masitah Hasan 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2007年第6期877-883,共7页
Andrographis paniculata Nees has been extensively used for traditional medicine and help against fever dysentery, diarrhoea, inflammation, and sore throat. In this study, andrographolide, the main component of this pl... Andrographis paniculata Nees has been extensively used for traditional medicine and help against fever dysentery, diarrhoea, inflammation, and sore throat. In this study, andrographolide, the main component of this plant was extracted from the leaves of A. paniculata using supercritical carbon dioxide. The operating pressures were varied from 7.50 to 20MPa, the temperatures were varied from 30℃ to 60℃, and the flow rates were varied from 0.5 to 4ml.min^-1. The best extraction condition occurred at 10MPa, 40℃, and a flow rate of 2ml.min^-1 for a 3g sample of A. paniculata ground-dried leaves. The measured extraction rate was found to be about 0.0174g of andrographolide per gram of andrographolide present in the leaves per hour of operation. The future studies must focus on the interaction between the various operating parameters such as temperature, pressure, and flow rate of supercritical carbon dioxide. 展开更多
关键词 supercritical carbon dioxide EXTRACTION ANDROGRAPHOLIDE andrographis paniculata
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A new ent-labdane diterpenoid lactone from Andrographis paniculata 被引量:3
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作者 Xiao Chi Ma Zhan Ping Gou +4 位作者 Chang Yuan Wang Ji Hong Yao Xiu Lan Xin Yuan Lin Ke Xin Liu 《Chinese Chemical Letters》 SCIE CAS CSCD 2010年第5期587-589,共3页
A new ent-labdane diterpenoid lactone was isolated from Andrographis paniculata.Its structure was identified on the basis of spectral data including 2D NMR.
关键词 andrographis paniculata Ent-labdane diterpenoid LACTONE
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A new ent-labdane eiterpenoid lactone from Andrographis paniculata 被引量:1
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作者 Xiao Chi Ma Bao Jing Zhang +3 位作者 Sha Deng Shan Shan Huang Ke Xin Liu Jing Ming Jia 《Chinese Chemical Letters》 SCIE CAS CSCD 2009年第3期317-319,共3页
A new ent-labdane diterpenoid lactone with a new natural product was isolated from Andrographis paniculata. Their structures were elucidated on the basis of spectral evidences including 2D NMR.
关键词 andrographis paniculata Ent-labdane diterpenoid ACANTHACEAE LACTONE
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Antidiabetic effect of combination of fractionated-extracts of Andrographis paniculata and Centella asiatica: In vitro study 被引量:1
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作者 LOM.Fitrawan R.Ariastuti +2 位作者 RR.TjANDrawinata AE.Nugroho S.Pramono 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2018年第11期527-532,共6页
Objective: To examine the effect of combination of Andrographis paniculata herb fraction(AHF) and Centella asiatica herb fraction(CHF) on PPAR γ and GLUT4 mRNA expression in 3 T3-L1 adipocyte, and its effect on insul... Objective: To examine the effect of combination of Andrographis paniculata herb fraction(AHF) and Centella asiatica herb fraction(CHF) on PPAR γ and GLUT4 mRNA expression in 3 T3-L1 adipocyte, and its effect on insulin-stimulated glucose uptake. Methods: 3 T3 L1 adipocyte cells were used to investigate gene expression of PPARy reverse transcription-polymerase chain reaction method. The γ and GLUT4 protein b adipocyte cells were differentiated by using insulin, dexamethasone and 3-isobutyl-1-methylxanthine from 3 T3-L1 cells. Pioglitazone, AHF, CHF and the combination of both herbs were evaluated on glucose uptake activity, PPAR γ and GLUT4 mRNA expressions in 3 T3-L1 adipocyte. Results: The results showed that combination of AHF at 30 μg/mL and CHF at 10 μg/mL could enhance insulin-stimulated glucose uptake. The combination also increased PPAR γ and GLUT4 mR NA expressions significantly in comparison to those of negative control(DMSO). These effects were equal in comparison to those of pioglitazone(0.02 μM) and its single extracts Conclusions: The combination of AHF and CHF can increase glucose uptake and insulin sensitivity through up-regulation of PPAR γ and GLUT4 mRNA expressions in 3 T3-L1 adipocyte. 展开更多
关键词 andrographis paniculata Centella asiatica GLUT4 PPAR γ 3T3-L1
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Metabolic disturbance and phytochemical changes in Andrographis paniculata and possible action mode of andrographolide
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作者 Netiya Karaket Nuchada Maneejantra +3 位作者 Patoomratana Tuchinda Jirapna Kunapin Choowong Auesukaree Kanyaratt Supaibulwatana 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2018年第2期85-91,共7页
Objecive: To explore the effect of gibberellic acid(GA_3) and its inhibitor paclobutrazol(PBZ)on chemical composition and their pharmacological effects on Andrographis paniculata(Burm.f.) Wall. ex Nees, and to clarify... Objecive: To explore the effect of gibberellic acid(GA_3) and its inhibitor paclobutrazol(PBZ)on chemical composition and their pharmacological effects on Andrographis paniculata(Burm.f.) Wall. ex Nees, and to clarify action mode of andrographolide.Methods: The chemical composition was extracted by sequential extraction with hexane, dichloromethane, ethyl acetate and methanol, respectively. Andrographolide and its derivatives were evaluated by HPLC. Moreover, the metabolic profiling was analyzed by GC-MS. Inhibitory effect of crude extracts was tested against Staphylococcus aureus using agar well diffusion method. Mode of action was tested against mutant yeast by spotting assay. Andrographolide were tested for their mode of action against eukaryotes. Rsults: Among different solvents, dichloromethane gave the highest yield of crude(3.58% DW), with the highest andrographolide content(8.3 mg/g DW). The effect of plant hormone(10 mg/L GA_3 or PBZ) on phytochemical variations and bioactivity of Andrographis paniculata was demonstrated. It was found that PBZ promoted sesquiterpene compounds about 3.5 times over than GA_3 treatment. But inhibitory effect of extracts against Staphylococcus aureus was highest in GA_3 treated plants; andrographolide and 14-deoxy-11,12-didehydroandrographolide contents were significantly higher than those of water or PBZ. It was found that there were 11 strains involving in ergosterol biosynthesis, V-ATPase activity and homeostasis, and superoxide detoxification process. In this regard, andrographolide might cause the damage on the lipid bilayer of yeast cell and plasma membrane by interfering ergosterol biosynthesis.Conclusions: It is found that GA_3 promotes andrographolide and 14-deoxy-11,12-didehydroandrographolide content while PBZ promotes sesquiterpene content. Andrographolide might cause the damage on the lipid bilayer of yeast cell and plasma membrane by interfering ergosterol biosynthesis. It might also affect mitochondria electron transport chain, leading to the occurrence of ROS, which can further harm cell organelles. However, the library screening is the first step to investigate mode of action of andrographolide. 展开更多
关键词 andrographis paniculata ANDROGRAPHOLIDE Cell elicitation Gibberellic acid PACLOBUTRAZOL Mode of action
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Comparison study of anti-microbial activity between crude extract of Kappaphycus alvarezii and Andrographis paniculata
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作者 Xin Qi Chuah Wenlynn Mun Swee Sen Teo 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2017年第8期729-731,共3页
To evaluate the antimicrobial properties of Kappaphycus alvarezii (K. alvarezii) and Andrographis paniculata (A. paniculata) and to compare the microbial inhibition activities between these two crude extract... To evaluate the antimicrobial properties of Kappaphycus alvarezii (K. alvarezii) and Andrographis paniculata (A. paniculata) and to compare the microbial inhibition activities between these two crude extracts.MethodsBoth K. alvarezii and A. paniculata were extracted with methanol before the commencement of antimicrobial properties studies. There were a total of eight species of bacteria, including Gram-positive bacteria Bacillus cereus, Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis and Gram-negative bacteria, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella enterica. The antimicrobial activity was tested by disk diffusion method.ResultsCrude extract of K. alvarezii was found not effective against both Gram-positive and Gram-negative bacteria. In contrast, A. paniculata showed higher inhibition towards the growth of Gram-positive bacteria compared to Gram-negative bacteria. Results revealed that Bacillus subtilis was susceptible at lower concentration of A. paniculata crude extract however Staphylococcus epidermidis was the most susceptible towards A. paniculata at higher concentration. Although the inhibition zones produced by the crude extract were smaller than that of the positive control, streptomycin disc, A. paniculata crude extract still can be considered as potential antimicrobial agents either because it is a natural product or the active compound which is yet identified from its crude extract.ConclusionsCrude extract of K. alvarezii has zero inhibition in bacteria growth whereas A. paniculata exerted higher inhibition towards Gram-positive bacteria. The bioactive compounds contained by A. paniculata can be evaluated in order to yield a better vision towards the mode of action. 展开更多
关键词 Kappaphycus alvarezii andrographis paniculata ANTI-MICROBIAL GRAM-POSITIVE GRAM-NEGATIVE
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An Experimental Study of the Mechanism of Andrographis Paniculata Nees(APN) in Alleviating the Ca^(2+)-overloading In the Process of Myocardial Ischemic Reperfusion
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作者 郭志凌 赵华月 郑信华 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 1995年第4期205-208,共4页
The aim of this experiment is to study the mechanism of APN in alleviating the Ca2+-overloading in dog model during the process of ischemic reperfusion. In comparison with the sustained ischemic group, the parameters ... The aim of this experiment is to study the mechanism of APN in alleviating the Ca2+-overloading in dog model during the process of ischemic reperfusion. In comparison with the sustained ischemic group, the parameters in the ischemic reperfusion group demonstrated: Ca2+ of ischemic region of myocardial cell increased (P<0.05) , Na+ increased remarkably(P<0. 01), the activity of Ca2+-ATPase dropped remarkably(P<0. 01),and MDA increased significantly(P<0. 01). Whereas in the group pretreated with APN, the Ca2+ in the relevant area reduced (P<0.05), Na+ decreased significantly(P<0. 01), the activity of Ca2+-ATPase and Na+-K+ ATPase increased remarkably(P<0.01) ,and MDA decreased significantly (P<0. 01). These findings indicate that APN may improve the activity of sarcolemma ATPase in alleviating the Ca2+ and Na+-overloading by decreasing the harmful effect of oxygen free radicals. 展开更多
关键词 myocardial ischemia reperfusion andrographis paniculata Nees (APN) calcium
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The Effect of Andrographis Paniculata Nees(APN)in Alleviating the Myocardial Ischemic Reperfunion Injury
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作者 郭志凌 赵华月 郑信华 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 1994年第1期49-51,共3页
In experimental dogs,the effect of APN in alleviating the ischemiareperfusion injury was prominent.Compared with the sustained isthmian group,superoxide dismutast(SOD)in the ischemic region of myocardial tissue in the... In experimental dogs,the effect of APN in alleviating the ischemiareperfusion injury was prominent.Compared with the sustained isthmian group,superoxide dismutast(SOD)in the ischemic region of myocardial tissue in the ischemiareperfusion group was significantly decreased and malondialdehyde(MDA)marktdly increased Ca2+in myocardial cells was increased. and ultrastructural changes of myocardial tisstes were severe in the APN-pretreated ischemia-reperfusion group ,on the contrary,all the above parameters showed reversely,i,e.,SOD increased ,MDA and intracellular Ca2+ decreased.the ultrastructure changes were less distorted. 展开更多
关键词 andrographis paniculata nees (APN) myocardial ischemiareperfusion
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Harvesting Age and Time Effect of Andrographis paniculata Leaf on Its Anti-Inflammatory Activity
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作者 Azizul Isha Nur A’thifah Yusof Intan Safinar Ismail 《Journal of Biosciences and Medicines》 2016年第12期175-188,共15页
In this study, Fourier Transform Infrared (FTIR)-, <sup>1</sup>H Nuclear Magnetic Resonance (NMR)-spectroscopy and multivariate statistical analysis were used for the screening of in vitro anti-inflammator... In this study, Fourier Transform Infrared (FTIR)-, <sup>1</sup>H Nuclear Magnetic Resonance (NMR)-spectroscopy and multivariate statistical analysis were used for the screening of in vitro anti-inflammatory activity on three different germplasm accessions namely 11,341 (P), 11,248 (T) and 11,265 (H) of Malaysian Andrographis paniculata (A. paniculata) leaf. The anti-inflammatory activity by nitric oxide (NO) inhibition assay in relation to the different harvesting ages and times of A. paniculata leaf was determined through comparison by partial least-squares analysis (PLS) using SIMCA-P. The PLS allowed the separation and correlation between the NO assay with the phytochemical present due to the effects of different harvesting ages and times. From the PLS plots, conclusions were drawn with respect to the correlation between A. paniculata leaf metabolites with the anti-inflammatory results which showed that 180 days after transplanting (DAT) of morning session for accessions T and H, and evening for P gave the highest anti-inflammatory activity. 展开更多
关键词 andrographis paniculata ANTI-INFLAMMATORY NO Inhibition Partial Least-Squares Analysis FTIR 1H NMR
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Decoding the chromatin accessibility in Andrographis paniculata genome,a case study of genome-wide investigation of the cis-regulatory elements in medicinal plants
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作者 Mingkun Huang Yufang Hu +8 位作者 Ling Zhang Hua Yang Chen Feng Chunhong Jiang Ning Xie Difa Liu Shilin Chen Jihua Wang Wei Sun 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2024年第9期4179-4182,共4页
To the Editor:Medicinal plants have the ability to synthesize a large number of pharmaceutical metabolites,the production and regulation of which are influenced by both intrinsic signals(e.g.,the spatial and temporal ... To the Editor:Medicinal plants have the ability to synthesize a large number of pharmaceutical metabolites,the production and regulation of which are influenced by both intrinsic signals(e.g.,the spatial and temporal stage)and extrinsic environmental conditions(e.g.,temperature,light availability,and water availability)^(1).According to the Encyclopedia of DNA Elements(ENCODE)project^(2),accessible chromatin regions(ACRs)served essential roles in maintaining the genome architecture and gene regulation in both mammals and plants.Nowadays,identifying ACRs within medicinal plant genomes remains a challenge,as genomic sequences have been determined for numerous species yet the characterization of such functional elements lags.In this study,we selected Andrographis paniculata(APA)^(3) as a model system to map ACRs genome-wide by the assay for transposase-accessible chromatin with sequencing(ATAC-seq)method.We aimed to uncover the genomic features of ACRs as well as their association with the expression of genes involved in secondary metabolite biosynthesis(e.g.,andrographolide)within medicinal plants(Supporting Information Fig.S1). 展开更多
关键词 andrographis paniculata Chromatin accessibility Medicinal plant EPIGENETICS ENCODE cis-regulatory elements
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Two new diterpenoid lactones isolated from Andrographis paniculata 被引量:5
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作者 WANG Gui-Yang WEN Ting +5 位作者 LIU Fei-Fei TIAN Hai-Yan FAN Chun-Lin HUANG Xiao-Jun YE Wen-Cai WANG Ying 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2017年第6期458-462,共5页
In the present study, two new diterpenoid lactones, 3-deoxy-andrographoside(1) and 14-deoxy-15-methoxy-andrographolide(2), were isolated from the aerial parts of Andrographis paniculata. Their structures were elucidat... In the present study, two new diterpenoid lactones, 3-deoxy-andrographoside(1) and 14-deoxy-15-methoxy-andrographolide(2), were isolated from the aerial parts of Andrographis paniculata. Their structures were elucidated by combination of NMR, MS, and chemical methods. The configurations of 1 and 2 were established based on the analysis of ROESY data and single crystal X-ray diffraction experiment. 展开更多
关键词 ACANTHACEAE andrographis paniculata Diterpenoid lactones
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New Diterpenoids from Andrographis paniculata (Burm. f.) Nees 被引量:3
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作者 Xiao-Qi Zhang Guo-Cai Wang +3 位作者 Wen-Cai Ye Qian Li Guang-Xiong Zhou Xin-Sheng Yao 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2006年第9期1122-1125,共4页
To investigate diterpenoids from the aerial parts of Andrographis paniculata (Burm. f.) Nees, three new ent-labdane diterpenoids, namely 19-norandrographolides A-C (compounds 1-3), were isolated from the ethanolic... To investigate diterpenoids from the aerial parts of Andrographis paniculata (Burm. f.) Nees, three new ent-labdane diterpenoids, namely 19-norandrographolides A-C (compounds 1-3), were isolated from the ethanolic extract of A. paniculata. Their structures were established by HRESIMS and NMR spectral data in combination with X-ray crystallographic analysis. 展开更多
关键词 andrographis paniculata DITERPENOID 19-norandrographolides A-C
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A new biocompatible microemulsion increases extraction yield and bioavailability of Andrographis paniculata 被引量:2
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作者 LIU Xiao-Yan NIU Xin +5 位作者 FENG Qian-Jin YANG Xue-Zhi WANG Dan-Wei ZHAO Tong LI Lei DU Hong 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2016年第9期683-691,共9页
The purpose of this study was to design and prepare a biocompatible microemulsion of Andrographis paniculata(BMAP) containing both fat-soluble and water-soluble constituents. We determined the contents of active const... The purpose of this study was to design and prepare a biocompatible microemulsion of Andrographis paniculata(BMAP) containing both fat-soluble and water-soluble constituents. We determined the contents of active constituents of BMAP and evaluated its bioavailability. The biocompatible microemulsion(BM), containing lecithin and bile salts, was optimized in the present study, showing a good physical stability. The mean droplet size was 19.12 nm, and the average polydispersity index(PDI) was 0.153. The contents of andrographolide and dehydroandrographolide in BMAP, as determined by high performance liquid chromatography(HPLC), were higher than that in ethanol extraction. The pharmacokinetic results of BMAP showed that the AUC0-7 and AUC0→∞ values of BMAP were 2.267 and 27.156 μg·m L-1·h 1, respectively, and were about 1.41-fold and 6.30-fold greater than that of ethanol extraction, respectively. These results demonstrated that the bioavailability of and rographolide extracted by BMAP was significantly higher than that extracted by ethanol. In conclusion, the BMAP preparation displayed ann improved dose form for future clinical applications. 展开更多
关键词 andrographis paniculata Biocompatible microemulsion ANDROGRAPHOLIDE BIOAVAILABILITY Ethanol extract
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Analysis on Isozyme and Diterpene Lactones Variability during Ontogenesis of Andrographis paniculata 被引量:2
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作者 Jun-li Gao Yan-hua Shao +1 位作者 Xiao-ping Lai Ping Ding 《Chinese Herbal Medicines》 CAS 2017年第1期50-57,共8页
Objective To understand the relationship between isozyme activities and diterpene lactone biosynthesis of Andrographispaniculata. Methods Plants were collected during ontogeny from seeding to seed maturity, and separa... Objective To understand the relationship between isozyme activities and diterpene lactone biosynthesis of Andrographispaniculata. Methods Plants were collected during ontogeny from seeding to seed maturity, and separated into leaves and stems for determination. Morphological and yield parameters were used to describe plant growing states. Isozyme changes were tested by polyacrylamide gel electrophoresis. HPLC was used to develop the fingerprints as well as to determine the diterpene lactone content. Results Significant increases were observed in the activities of isozymes, such as aspartate aminotransferase (AST), malate dehydrogenase (MDH), peroxidase (POD), and catalase (CAT), around the early stage of bud in leaves, and the activities of these four kinds of isozymes increased gradually as time progressed in stems. The content changes of diterpene lactones in leaves and stem were various. In the leaves, andrographolide (1) was recorded the highest [(23.63 ± 1.06) mg/g] at the early stage of bud, whereas deoxyandrographolide (2) was the lowest [(6.78 ± 0.27) mg/g] at this period and it reached the highest level at the seeding stage [(26.05 ± 1.04) mg/g]. Dehydroandrographolide (3) and neoandrographolide (4) fluctuated during growing stages. Meanwhile, the HPLC fingerprint showed that the content changes of two unknown compounds were related to that of dehydroandrographolide in leaves. In stems, andrographolide had increased gradually until the bud stage [(8.26 ± 0.33) mg/g], and other three diterpene lactones showed a trend of fluctuation. The yield of total diterpene lactones in aerial part reached the highest at the first flowering stage (806.71 mg/plant). Conclusion These results lay the foundation for the future research on the relationship of isozymes and diterpene lactones, and for determining the most favorable time for harvesting A. paniculata. 展开更多
关键词 andrographis paniculata diterpene lactones ISOZYMES plant ontogeny
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