The aim of this experiment is to study the mechanism of APN in alleviating the Ca2+-overloading in dog model during the process of ischemic reperfusion. In comparison with the sustained ischemic group, the parameters ...The aim of this experiment is to study the mechanism of APN in alleviating the Ca2+-overloading in dog model during the process of ischemic reperfusion. In comparison with the sustained ischemic group, the parameters in the ischemic reperfusion group demonstrated: Ca2+ of ischemic region of myocardial cell increased (P<0.05) , Na+ increased remarkably(P<0. 01), the activity of Ca2+-ATPase dropped remarkably(P<0. 01),and MDA increased significantly(P<0. 01). Whereas in the group pretreated with APN, the Ca2+ in the relevant area reduced (P<0.05), Na+ decreased significantly(P<0. 01), the activity of Ca2+-ATPase and Na+-K+ ATPase increased remarkably(P<0.01) ,and MDA decreased significantly (P<0. 01). These findings indicate that APN may improve the activity of sarcolemma ATPase in alleviating the Ca2+ and Na+-overloading by decreasing the harmful effect of oxygen free radicals.展开更多
In experimental dogs,the effect of APN in alleviating the ischemiareperfusion injury was prominent.Compared with the sustained isthmian group,superoxide dismutast(SOD)in the ischemic region of myocardial tissue in the...In experimental dogs,the effect of APN in alleviating the ischemiareperfusion injury was prominent.Compared with the sustained isthmian group,superoxide dismutast(SOD)in the ischemic region of myocardial tissue in the ischemiareperfusion group was significantly decreased and malondialdehyde(MDA)marktdly increased Ca2+in myocardial cells was increased. and ultrastructural changes of myocardial tisstes were severe in the APN-pretreated ischemia-reperfusion group ,on the contrary,all the above parameters showed reversely,i,e.,SOD increased ,MDA and intracellular Ca2+ decreased.the ultrastructure changes were less distorted.展开更多
Objective:Andrographis paniculata is a well-known medicinal plant in Southeast Asia,India and China.The plant contains andrographolide(AN),a very important phytochemical used in various health problems.However,AN is l...Objective:Andrographis paniculata is a well-known medicinal plant in Southeast Asia,India and China.The plant contains andrographolide(AN),a very important phytochemical used in various health problems.However,AN is low in oral absorption bioavailability of AN due to the rapid clearance and high protein binding capacity.Methods:The present study was aimed to develop a nano-phytovesicular formulation of semi-purified AN extracts from a naturally occurring phospholipid(soya phosphatidylcholine)in order to increase the oral absorption and antihyperglycemic activity in rats.Results:The nano-phyto vesicle of semi-purified AN extracts equivalent to 25 mg/kg AN significantly protected the hyperglycemic condition of rats.The in vitro and in vivo experiments results proved that the nano-phytovesicular system of plant extracts containing AN produced better oral absorption,bioavailability and improved antihyperglycemic activity compared with that of free AN at dose of 50 mg/kg.Conclusion:Hence,the prepared semi-purified extract nano-phytovesicular system is helpful in solving the problem of rapid clearance of AN.展开更多
目的 :研究穿心莲是否具有体外破坏内毒素的作用。方法 :1电镜观察经穿心莲作用后的脂多糖(L PS)构型变化。 2观察穿心莲体外破坏 L PS的量效关系。 3观察穿心莲对 L PS刺激体外腹腔巨噬细胞分泌炎性细胞因子活性的影响。结果 :电镜观...目的 :研究穿心莲是否具有体外破坏内毒素的作用。方法 :1电镜观察经穿心莲作用后的脂多糖(L PS)构型变化。 2观察穿心莲体外破坏 L PS的量效关系。 3观察穿心莲对 L PS刺激体外腹腔巨噬细胞分泌炎性细胞因子活性的影响。结果 :电镜观察证实经穿心莲作用后的 L PS已失去其正常结构 ,呈散乱分布的残破网或短带状 ,而且穿心莲体外破坏 L PS作用在一定范围内随药物浓度的增加而提高。 1g穿心莲约可裂解836 EU的 L PS。同时 ,穿心莲能轻度增加体外正常腹腔巨噬细胞分泌肿瘤坏死因子 α(TNFα)、白介素 1(IL 1)和 IL 6的活性 ,显著抑制经 L PS刺激体外腹腔巨噬细胞分泌大量上述炎性细胞因子的活性 ,其作用在一定范围内呈剂量依赖性。结论 :穿心莲在体外可通过直接破坏内毒素结构和抑制其所介导释放的大量炎性细胞因子活性起到拮抗内毒素的作用。展开更多
文摘The aim of this experiment is to study the mechanism of APN in alleviating the Ca2+-overloading in dog model during the process of ischemic reperfusion. In comparison with the sustained ischemic group, the parameters in the ischemic reperfusion group demonstrated: Ca2+ of ischemic region of myocardial cell increased (P<0.05) , Na+ increased remarkably(P<0. 01), the activity of Ca2+-ATPase dropped remarkably(P<0. 01),and MDA increased significantly(P<0. 01). Whereas in the group pretreated with APN, the Ca2+ in the relevant area reduced (P<0.05), Na+ decreased significantly(P<0. 01), the activity of Ca2+-ATPase and Na+-K+ ATPase increased remarkably(P<0.01) ,and MDA decreased significantly (P<0. 01). These findings indicate that APN may improve the activity of sarcolemma ATPase in alleviating the Ca2+ and Na+-overloading by decreasing the harmful effect of oxygen free radicals.
文摘In experimental dogs,the effect of APN in alleviating the ischemiareperfusion injury was prominent.Compared with the sustained isthmian group,superoxide dismutast(SOD)in the ischemic region of myocardial tissue in the ischemiareperfusion group was significantly decreased and malondialdehyde(MDA)marktdly increased Ca2+in myocardial cells was increased. and ultrastructural changes of myocardial tisstes were severe in the APN-pretreated ischemia-reperfusion group ,on the contrary,all the above parameters showed reversely,i,e.,SOD increased ,MDA and intracellular Ca2+ decreased.the ultrastructure changes were less distorted.
基金financial support through UGC-JRF in Engineering and Technology(Vide-F No.10-01/2008 SA-I)。
文摘Objective:Andrographis paniculata is a well-known medicinal plant in Southeast Asia,India and China.The plant contains andrographolide(AN),a very important phytochemical used in various health problems.However,AN is low in oral absorption bioavailability of AN due to the rapid clearance and high protein binding capacity.Methods:The present study was aimed to develop a nano-phytovesicular formulation of semi-purified AN extracts from a naturally occurring phospholipid(soya phosphatidylcholine)in order to increase the oral absorption and antihyperglycemic activity in rats.Results:The nano-phyto vesicle of semi-purified AN extracts equivalent to 25 mg/kg AN significantly protected the hyperglycemic condition of rats.The in vitro and in vivo experiments results proved that the nano-phytovesicular system of plant extracts containing AN produced better oral absorption,bioavailability and improved antihyperglycemic activity compared with that of free AN at dose of 50 mg/kg.Conclusion:Hence,the prepared semi-purified extract nano-phytovesicular system is helpful in solving the problem of rapid clearance of AN.
文摘目的 :研究穿心莲是否具有体外破坏内毒素的作用。方法 :1电镜观察经穿心莲作用后的脂多糖(L PS)构型变化。 2观察穿心莲体外破坏 L PS的量效关系。 3观察穿心莲对 L PS刺激体外腹腔巨噬细胞分泌炎性细胞因子活性的影响。结果 :电镜观察证实经穿心莲作用后的 L PS已失去其正常结构 ,呈散乱分布的残破网或短带状 ,而且穿心莲体外破坏 L PS作用在一定范围内随药物浓度的增加而提高。 1g穿心莲约可裂解836 EU的 L PS。同时 ,穿心莲能轻度增加体外正常腹腔巨噬细胞分泌肿瘤坏死因子 α(TNFα)、白介素 1(IL 1)和 IL 6的活性 ,显著抑制经 L PS刺激体外腹腔巨噬细胞分泌大量上述炎性细胞因子的活性 ,其作用在一定范围内呈剂量依赖性。结论 :穿心莲在体外可通过直接破坏内毒素结构和抑制其所介导释放的大量炎性细胞因子活性起到拮抗内毒素的作用。
