Objective:To study the chemical constituents of the roots of Angelica dahurica,a well-known Chinese herbal medicine named Baizhi in Chinese.Methods:Compounds were separated by various chromatographies,and the structur...Objective:To study the chemical constituents of the roots of Angelica dahurica,a well-known Chinese herbal medicine named Baizhi in Chinese.Methods:Compounds were separated by various chromatographies,and the structures of new compounds were elucidated based on the analysis of their spectroscopic and spectrometric data(1D,2D NMR,HRESI MS,IR,and UV).The absolute configurations of new compounds were determined by the calculated electronic circular dichroism and chemical derivatization.The inhibitory activities of all isolates against nitric oxide(NO)production were evaluated using lipopolysaccharide-activated RAW 264.7macrophage cells.Results:Seven new 3,4-dihydro-furanocoumarin derivatives(1a/1b,2a/2b,3a/3b,4)together with a known furanocoumarin(5)were isolated from the roots of A.dahurica.The new compounds included three pairs of enantiomers,(4S,2’’R)-angelicadin A(1a)/(4R,2’’S)-angelicadin A(1b),(4S,2’’S)-angelicadin A(2a)/(4R,2’’R)-angelicadin A(2b),and(4S,2’’S)-secoangelicadin A(3a)/(4R,2’’R)-secoangelicadin A(3b),together with(4R,2’’R)-secoangelicadin A methyl ester(4).The known xanthotoxol(5)inhibited the NO production with the half-maximal inhibitory concentration(IC50)value of(32.8±0.8)μmol/L,but all the new compounds showed no inhibitory activities at the concentration of100μmol/L.Conclusion:This is the first report of the discovery of 3,4-dihydro-furanocoumarins from A.dahurica.The results are not only meaningful for the understanding of the chemical constituents of A.dahurica,but also enrich the reservoir of natural products.展开更多
Objective： To investigate the chemical constituents from the roots of Angelica dahurica.Methods： The chemical constituents were isolated and purified by AB-8 macroporous adsorption resin and Sephadex LH-20 column as...Objective： To investigate the chemical constituents from the roots of Angelica dahurica.Methods： The chemical constituents were isolated and purified by AB-8 macroporous adsorption resin and Sephadex LH-20 column as well as semi-preparative reversed phase HPLC.The chemical structures were identified by spectral data.Results： Ten compounds were isolated and identified as xanthoarnol-3-O-β-d-glucopyranoside（1）,angedahuricoside A（2）,angedahuricoside B（3）,isofraxidin-7-O-β-d-glucopyranoside（4）,fraxidin-8-O-β-d-glucopyranoside（5）,（-）-marmesinin（6）,（2 S,3 R）-3-hydroxymarmesinin（7）,hyuganoside V（8）,daucosterol（9）,and sucrose（10）.Conclusion： Compounds 1 –3 are new ones and compounds 4 –6 and 8 are obtained from title plant for the first time.展开更多
基金financially supported by Beijing Natural Science Foundation(No.7202125)。
文摘Objective:To study the chemical constituents of the roots of Angelica dahurica,a well-known Chinese herbal medicine named Baizhi in Chinese.Methods:Compounds were separated by various chromatographies,and the structures of new compounds were elucidated based on the analysis of their spectroscopic and spectrometric data(1D,2D NMR,HRESI MS,IR,and UV).The absolute configurations of new compounds were determined by the calculated electronic circular dichroism and chemical derivatization.The inhibitory activities of all isolates against nitric oxide(NO)production were evaluated using lipopolysaccharide-activated RAW 264.7macrophage cells.Results:Seven new 3,4-dihydro-furanocoumarin derivatives(1a/1b,2a/2b,3a/3b,4)together with a known furanocoumarin(5)were isolated from the roots of A.dahurica.The new compounds included three pairs of enantiomers,(4S,2’’R)-angelicadin A(1a)/(4R,2’’S)-angelicadin A(1b),(4S,2’’S)-angelicadin A(2a)/(4R,2’’R)-angelicadin A(2b),and(4S,2’’S)-secoangelicadin A(3a)/(4R,2’’R)-secoangelicadin A(3b),together with(4R,2’’R)-secoangelicadin A methyl ester(4).The known xanthotoxol(5)inhibited the NO production with the half-maximal inhibitory concentration(IC50)value of(32.8±0.8)μmol/L,but all the new compounds showed no inhibitory activities at the concentration of100μmol/L.Conclusion:This is the first report of the discovery of 3,4-dihydro-furanocoumarins from A.dahurica.The results are not only meaningful for the understanding of the chemical constituents of A.dahurica,but also enrich the reservoir of natural products.
基金The National Natural Science Foundation of China (81473321)Beijing Municipal Natural Science Foundation (7152086)+1 种基金the National Key Technology R & D Program of China (2011BAI07B082012BAI29B02)
文摘Objective： To investigate the chemical constituents from the roots of Angelica dahurica.Methods： The chemical constituents were isolated and purified by AB-8 macroporous adsorption resin and Sephadex LH-20 column as well as semi-preparative reversed phase HPLC.The chemical structures were identified by spectral data.Results： Ten compounds were isolated and identified as xanthoarnol-3-O-β-d-glucopyranoside（1）,angedahuricoside A（2）,angedahuricoside B（3）,isofraxidin-7-O-β-d-glucopyranoside（4）,fraxidin-8-O-β-d-glucopyranoside（5）,（-）-marmesinin（6）,（2 S,3 R）-3-hydroxymarmesinin（7）,hyuganoside V（8）,daucosterol（9）,and sucrose（10）.Conclusion： Compounds 1 –3 are new ones and compounds 4 –6 and 8 are obtained from title plant for the first time.
文摘目的比较不同加工方法及干燥温度对白芷Angelicae Dahuricae Radix饮片外观及香豆素类成分的影响,明确不同加工方法适宜的干燥温度,筛选适宜的白芷产地加工方法。方法采用烘干蒸切法、半鲜切干燥法及鲜切干燥法结合3个干燥温度(40、50、60℃)对新鲜白芷进行加工。采用色差仪量化白芷饮片颜色,HPLC法测定香柑内酯、氧化前胡素、欧前胡素、珊瑚菜素、异欧前胡素5个香豆素成分的含量。利用系统聚类法判定样品颜色和成分差异,基于灰色关联度法探索颜色与成分的关联性,采用秩和比综合评价法(rank sum ratio comprehensive evaluation method,RSR)评价样品质量,并利用环氧化酶-2(cyclooxygenase-2,COX-2)抑制剂筛选试剂盒测定样品体外抗炎活性。结果亮度(L^(*))值以50℃干燥样品最高,60℃干燥样品次之,40℃干燥样品最低,黄蓝(b^(*))值相反。香豆素总量及氧化前胡素含量随干燥温度上升而增加,60℃鲜切干燥样品各成分含量最高,40℃烘干蒸切样品最低。颜色、成分聚类结果均显示40℃烘干蒸切样品及60℃鲜切干燥样品与其余样品差异极明显。灰色关联度结果显示L^(*)值与氧化前胡素、欧前胡素关联性较强;b^(*)值与珊瑚菜素、欧前胡素关联性较强,红绿(a^(*))值与各指标成分关联程度均较低。质量评价结果显示排名前3的样品分别为60℃鲜切干燥样品、50℃鲜切干燥样品、60℃半鲜切干燥样品,40℃烘干蒸切饮片排名最低;体外抗炎活性测定结果与质量评价结果相近,且半数抑制浓度(50%inhibitory concentration,IC_(50))与除佛手柑内酯外的各香豆素类成分均呈显著负相关。结论加工方法及干燥温度对白芷饮片影响显著,其中60℃半鲜切干燥饮片及50℃鲜切干燥饮片颜色较白,抗炎活性较高,生产效率高,是值得推广的白芷饮片。
