To explore the relationship of angiogenesis-related angiopoietin-2 gene and its receptor Tie2 with angiogenesis and the biology of hepatocellular carcinoma (HCC), angiopoietin-2 gene, Tie2 and CD34 protein expression ...To explore the relationship of angiogenesis-related angiopoietin-2 gene and its receptor Tie2 with angiogenesis and the biology of hepatocellular carcinoma (HCC), angiopoietin-2 gene, Tie2 and CD34 protein expression in 22 resected HCC, 8 cirrhotic and 8 control liver specimens were investigated by in situ hybridization and immunohistochemistry respectively, and the level of angiopoietin-2 and Tie2 expression in HCC were compared in terms of tumor biological parameters. It was found that CD34 was not expressed in control liver, expressed scarcely in cirrhotic liver (17 8±13 5/HP), but intensively expressed in HCC (86. 3±34. 8/HP, P<0. 01). Tie2 receptor was not expressed in controls, expressed at low level in cirrhotic liver (11. 3±8. 7/HP), while strongly positive in the microvascular endothelia of HCC (52. 4±16. 7/HP, P<0. 01). The level of Tie2 receptor expression in HCC was closely related with tumor diameter, angiogenesis and portal invasion. Angiopoietin-2 gene was not expressed in control liver, expressed mildly in cirrhotic liver (11. 2±9. 7/HP), but extensively in tumor zone (36. 4±17. 5/HP), the level of angiopoietin-2 expression was closely related with angiogenesis, portal invasion and histological grading of HCC. It is concluded that angiogenesis is increased in HCC; angiopoietin-2/Tie2 expression in human hepatic carcinoma is closely related with angiogenesis, which are probably involved in the HCC angiogenesis regulation, promoting the development and metastasis of human hepatic cancer.展开更多
·AIM: To determine the effects of laser photocoagulation on serum levels of angiopoietin-1(Ang-1),angiopoietin-2(Ang-2), soluble angiopoietin receptor Tie-2(Tie-2), Ang-1/Ang-2 ratio and vascular endothelial grow...·AIM: To determine the effects of laser photocoagulation on serum levels of angiopoietin-1(Ang-1),angiopoietin-2(Ang-2), soluble angiopoietin receptor Tie-2(Tie-2), Ang-1/Ang-2 ratio and vascular endothelial growth factor(VEGF) in patients with type 2diabetes mellitus(T2DM) and proliferative diabetic retinopathy(PDR). We also explored the role of the Ang/Tie system in PDR.·METHODS:Totally 160patientswithT2 DM, including50 patients with non-diabetic retinopathy(NDR), 58 patients with non-proliferative diabetic retinopathy(NPDR), and52 patients with PDR were enrolled in this study. Serum Ang-1, Ang-2, Tie-2 receptor and VEGF levels were measured using enzyme-linked immunosorbent assays for all patients and were repeated in 26 patients who underwent laser photocoagulation two months after the procedure.·RESULTS:ThemedianlevelsofAng-2andVEGFinserum were significantly higher in the NPDR group(4.23 ng/mL and 303.2 pg/mL, respectively) compared to the NDR group(2.67 ng/mL and 159.8 pg/mL, respectively, P 【0.01), with the highest level in the PDR group(6.26 ng/mL and531.2 pg/mL, respectively, P 【0.01). The median level of Ang-1 was significantly higher in the NPDR group(10.77ng/mL) compared to the NDR group(9.31 ng/mL) and the PDR groups(9.54 ng/mL)(P 【0.05), while no difference was observed between the PDR and NDR groups. Ang-1/Ang-2 ratio of PDR group was lowest in three groups(1.49 vs 2.69 and 2.90, both P 【0.01). The median level of Tie-2was not significantly different among three groups(P 】0.05).Ang-2 was positively correlated with VEGF and Tie-2 in the PDR and NPDR groups(both P 【0.05). Among the 26 patients who underwent laser photocoagulation, serum Ang-2 and VEGF levels significantly decreased(both P 【0.05), whereas serum Ang-1 level and Ang-1/Ang-2ratio were weakly increased(P 】0.05). The median levels of Ang-2 and VEGF in serum were highest in PDR group,however, Ang-1/Ang-2 ratio of PDR group was lowest in three groups.·CONCLUSION: Laser photocoagulation can reduce serum Ang-2 and VEGF levels. The Ang/Tie system and VEGF play an important role in the development and progression of T2 DM patients with PDR.展开更多
目的观察微波针刺配合生血润肤饮治疗寻常型银屑病的临床疗效。方法将100例寻常型银屑病患者随机分为对照组和观察组,每组50例。对照组采用口服生血润肤饮加减治疗,观察组在对照组基础上加用微波针刺治疗。观察两组治疗前后各项中医症候...目的观察微波针刺配合生血润肤饮治疗寻常型银屑病的临床疗效。方法将100例寻常型银屑病患者随机分为对照组和观察组,每组50例。对照组采用口服生血润肤饮加减治疗,观察组在对照组基础上加用微波针刺治疗。观察两组治疗前后各项中医症候(红斑、鳞屑、浸润增厚及皮损面积)积分、银屑病面积和严重程度指数(psoriasis area and severity index,PASI)评分、血清生物标志物[血管生成素2(angiopoietin 2,ANGPT2)、血管内皮生长因子受体3(vascular endothelial growth factor receptor-3,VEGFR-3)]水平及免疫功能各项指标(CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+))的变化情况,并比较两组临床疗效及不良反应发生率。结果观察组总有效率为96.0%,明显高于对照组的76.0%(P<0.05)。两组治疗后各项中医症候积分、PASI评分、CD8^(+)及血清ANGPT2、VEGFR-3水平均较同组治疗前显著下降,CD4^(+)、CD4^(+)/CD8^(+)均较同组治疗前显著上升,差异均具有统计学意义(P<0.05)。观察组治疗后各项中医症候积分、PASI评分、CD8^(+)及血清ANGPT2、VEGFR-3水平均明显低于对照组,CD4^(+)、CD4^(+)/CD8^(+)均明显高于对照组,差异均具有统计学意义(P<0.05)。对照组治疗期间不良反应发生率为4.0%,观察组为6.0%,两组比较差异无统计学意义(P>0.05)。结论微波针刺配合生血润肤饮是一种治疗寻常型银屑病的有效方法,能有效改善患者临床症状,降低血清ANGPT2及VEGFR-3水平,增强免疫功能,且安全可靠。展开更多
文摘To explore the relationship of angiogenesis-related angiopoietin-2 gene and its receptor Tie2 with angiogenesis and the biology of hepatocellular carcinoma (HCC), angiopoietin-2 gene, Tie2 and CD34 protein expression in 22 resected HCC, 8 cirrhotic and 8 control liver specimens were investigated by in situ hybridization and immunohistochemistry respectively, and the level of angiopoietin-2 and Tie2 expression in HCC were compared in terms of tumor biological parameters. It was found that CD34 was not expressed in control liver, expressed scarcely in cirrhotic liver (17 8±13 5/HP), but intensively expressed in HCC (86. 3±34. 8/HP, P<0. 01). Tie2 receptor was not expressed in controls, expressed at low level in cirrhotic liver (11. 3±8. 7/HP), while strongly positive in the microvascular endothelia of HCC (52. 4±16. 7/HP, P<0. 01). The level of Tie2 receptor expression in HCC was closely related with tumor diameter, angiogenesis and portal invasion. Angiopoietin-2 gene was not expressed in control liver, expressed mildly in cirrhotic liver (11. 2±9. 7/HP), but extensively in tumor zone (36. 4±17. 5/HP), the level of angiopoietin-2 expression was closely related with angiogenesis, portal invasion and histological grading of HCC. It is concluded that angiogenesis is increased in HCC; angiopoietin-2/Tie2 expression in human hepatic carcinoma is closely related with angiogenesis, which are probably involved in the HCC angiogenesis regulation, promoting the development and metastasis of human hepatic cancer.
文摘·AIM: To determine the effects of laser photocoagulation on serum levels of angiopoietin-1(Ang-1),angiopoietin-2(Ang-2), soluble angiopoietin receptor Tie-2(Tie-2), Ang-1/Ang-2 ratio and vascular endothelial growth factor(VEGF) in patients with type 2diabetes mellitus(T2DM) and proliferative diabetic retinopathy(PDR). We also explored the role of the Ang/Tie system in PDR.·METHODS:Totally 160patientswithT2 DM, including50 patients with non-diabetic retinopathy(NDR), 58 patients with non-proliferative diabetic retinopathy(NPDR), and52 patients with PDR were enrolled in this study. Serum Ang-1, Ang-2, Tie-2 receptor and VEGF levels were measured using enzyme-linked immunosorbent assays for all patients and were repeated in 26 patients who underwent laser photocoagulation two months after the procedure.·RESULTS:ThemedianlevelsofAng-2andVEGFinserum were significantly higher in the NPDR group(4.23 ng/mL and 303.2 pg/mL, respectively) compared to the NDR group(2.67 ng/mL and 159.8 pg/mL, respectively, P 【0.01), with the highest level in the PDR group(6.26 ng/mL and531.2 pg/mL, respectively, P 【0.01). The median level of Ang-1 was significantly higher in the NPDR group(10.77ng/mL) compared to the NDR group(9.31 ng/mL) and the PDR groups(9.54 ng/mL)(P 【0.05), while no difference was observed between the PDR and NDR groups. Ang-1/Ang-2 ratio of PDR group was lowest in three groups(1.49 vs 2.69 and 2.90, both P 【0.01). The median level of Tie-2was not significantly different among three groups(P 】0.05).Ang-2 was positively correlated with VEGF and Tie-2 in the PDR and NPDR groups(both P 【0.05). Among the 26 patients who underwent laser photocoagulation, serum Ang-2 and VEGF levels significantly decreased(both P 【0.05), whereas serum Ang-1 level and Ang-1/Ang-2ratio were weakly increased(P 】0.05). The median levels of Ang-2 and VEGF in serum were highest in PDR group,however, Ang-1/Ang-2 ratio of PDR group was lowest in three groups.·CONCLUSION: Laser photocoagulation can reduce serum Ang-2 and VEGF levels. The Ang/Tie system and VEGF play an important role in the development and progression of T2 DM patients with PDR.
文摘目的观察微波针刺配合生血润肤饮治疗寻常型银屑病的临床疗效。方法将100例寻常型银屑病患者随机分为对照组和观察组,每组50例。对照组采用口服生血润肤饮加减治疗,观察组在对照组基础上加用微波针刺治疗。观察两组治疗前后各项中医症候(红斑、鳞屑、浸润增厚及皮损面积)积分、银屑病面积和严重程度指数(psoriasis area and severity index,PASI)评分、血清生物标志物[血管生成素2(angiopoietin 2,ANGPT2)、血管内皮生长因子受体3(vascular endothelial growth factor receptor-3,VEGFR-3)]水平及免疫功能各项指标(CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+))的变化情况,并比较两组临床疗效及不良反应发生率。结果观察组总有效率为96.0%,明显高于对照组的76.0%(P<0.05)。两组治疗后各项中医症候积分、PASI评分、CD8^(+)及血清ANGPT2、VEGFR-3水平均较同组治疗前显著下降,CD4^(+)、CD4^(+)/CD8^(+)均较同组治疗前显著上升,差异均具有统计学意义(P<0.05)。观察组治疗后各项中医症候积分、PASI评分、CD8^(+)及血清ANGPT2、VEGFR-3水平均明显低于对照组,CD4^(+)、CD4^(+)/CD8^(+)均明显高于对照组,差异均具有统计学意义(P<0.05)。对照组治疗期间不良反应发生率为4.0%,观察组为6.0%,两组比较差异无统计学意义(P>0.05)。结论微波针刺配合生血润肤饮是一种治疗寻常型银屑病的有效方法,能有效改善患者临床症状,降低血清ANGPT2及VEGFR-3水平,增强免疫功能,且安全可靠。
