To study the effects of various concentrations of SmCl3 on the morphology and functions of porcine thyroid cells in vitro, the methods of the electron microscope and radioimmunoassay were used. The investigation showe...To study the effects of various concentrations of SmCl3 on the morphology and functions of porcine thyroid cells in vitro, the methods of the electron microscope and radioimmunoassay were used. The investigation showed that when the thyroid cells were cultured in the presence of 0.001 and 0.01 mmol/L SmCl3, the measured levels of tetraiodothyronine (T4) were much higher than those in control cells and iodine uptake by thyroid cells increased, and the cells appeared to be active functional morphology under the electron microscope. When the thyroid cells were cultured in the presence of 0.1 mmol/L SmCl3, the levels of T4 and iodine uptake by cells decreased as compared with those in control cells, and the morphological changes of the cells presented an inactive functional state. From the evidences mentioned above, it demonstrates that very low concentrations (0.001 and 0.01 mmol/L) of SmCl3 promote synthesis and secretion of thyroid hormone, whereas a higher concentration (0.1 mmol/L of SmCl3) inhibits the synthesis and secretion of thyroid hormone.展开更多
Gold nanoparticles （NPs） have highly efficient multi-photon-induced luminescence. In this paper, we record the two-photon images of gold NPs, lymphoma cell line Karpas 299, and Karpas 299 incubated with 30-nm-diamet...Gold nanoparticles （NPs） have highly efficient multi-photon-induced luminescence. In this paper, we record the two-photon images of gold NPs, lymphoma cell line Karpas 299, and Karpas 299 incubated with 30-nm-diameter gold NPs and ACT-1 antibody conjugates （Au30-ACT-1 conjugates） by using a multi-photon microscopy system. Due to the specific conjugation of ACT-1 antibody and cell membrane receptor CD25, gold NPs are only bound to the surface of cell membrane of Karpas 299. The luminescence intensity of gold NPs is higher than that of cells at 750-nm laser excitation. By comparing the images of Karpas 299 cells incubated with and without gold NPs, it is found that by means of gold NPs, we can get clear cell images with lower excitation power. Their excellent optical and chemical properties make gold NPs an attractive contrast agent for cellular imaging.展开更多
文摘To study the effects of various concentrations of SmCl3 on the morphology and functions of porcine thyroid cells in vitro, the methods of the electron microscope and radioimmunoassay were used. The investigation showed that when the thyroid cells were cultured in the presence of 0.001 and 0.01 mmol/L SmCl3, the measured levels of tetraiodothyronine (T4) were much higher than those in control cells and iodine uptake by thyroid cells increased, and the cells appeared to be active functional morphology under the electron microscope. When the thyroid cells were cultured in the presence of 0.1 mmol/L SmCl3, the levels of T4 and iodine uptake by cells decreased as compared with those in control cells, and the morphological changes of the cells presented an inactive functional state. From the evidences mentioned above, it demonstrates that very low concentrations (0.001 and 0.01 mmol/L) of SmCl3 promote synthesis and secretion of thyroid hormone, whereas a higher concentration (0.1 mmol/L of SmCl3) inhibits the synthesis and secretion of thyroid hormone.
基金supported by a grant from the National Natural Science Foundation of China under Grant No.60578026,60878056.
文摘Gold nanoparticles （NPs） have highly efficient multi-photon-induced luminescence. In this paper, we record the two-photon images of gold NPs, lymphoma cell line Karpas 299, and Karpas 299 incubated with 30-nm-diameter gold NPs and ACT-1 antibody conjugates （Au30-ACT-1 conjugates） by using a multi-photon microscopy system. Due to the specific conjugation of ACT-1 antibody and cell membrane receptor CD25, gold NPs are only bound to the surface of cell membrane of Karpas 299. The luminescence intensity of gold NPs is higher than that of cells at 750-nm laser excitation. By comparing the images of Karpas 299 cells incubated with and without gold NPs, it is found that by means of gold NPs, we can get clear cell images with lower excitation power. Their excellent optical and chemical properties make gold NPs an attractive contrast agent for cellular imaging.
