Potential of Binahong (<i>Anredera cordifolia</i>[Tenore] Steen) in Reducing TNF-<i>α</i>Expression on Regeneration of Pancreas <i>β</i>Cells on White Rats (<i>Rattus norvegicus</i>) Diabetes Mellitus Models

Diabetes mellitus (DM) is a metabolic disorder characterized by increased blood glucose levels (hyperglycemia) due to impaired insulin secretion and/or increased insulin resistance. Providing binahong leaf steeping (Anredera cordifolia [Tenore] Steen) is one alternative treatment for people with diabetes mellitus using medicinal plants. This research was conducted to find out the right concentration by administering binahong leaf steeping to reduce hyperglycemia in STZ induced rat and to analyze administration of binahong leaf steeping in reducing TNF-α expression in pancreatic β cell regeneration. This study used 25 white rats (Rattus novergicus) which were divided into 5 groups: group 1, negative control (K-), group 2, positive control (K+), groups 3, 4, and 5. With STZ induction, each is given a dose of binahong leaf steeping, 50.4 mg/180ml of water;100.8 mg/180ml of water and 151.2 mg/180ml of water for 14 days. The results showed that the administration of binahong leaf steeping to STZ induced rat could reduce glucose rats in a row by 130.00, respectively;117,23 and 112,45 and steeping binahong leaves (Anredera cordifolia [Ten] S) significantly influence the reduction in TNF-α expression in pancreatic β cell regeneration. Thus, this study can be concluded that the right dose to reduce hyperglycemic and regeneration of pancreatic β cells produced by STZ induced rats is 151.2 mg/180ml of water and statistically influences in reducing TNF-α expression in pancreatic β cell regeneration.

藤三七中总黄酮提取工艺的研究

目的:筛选藤三七中总黄酮的提取方法,为进一步研究总黄酮的最佳提取工艺,充分利用和开发藤三七资源提供科学依据。方法:采用分光光度法,以芦丁为标准品测定藤三七中总黄酮含量。在提取过程中通过单因素试验分析了乙醇浓度、料液比、回流时间及回流温度4个主要因素对提取率的影响。在单因素试验的基础上通过正交设计法优化藤三七黄酮提取工艺条件。结果:藤三七总黄酮的最佳提取工艺条件为乙醇体积浓度为40%,料液比为1∶20,回流温度为60℃,提取时间为2h。此条件下藤三七的总黄酮得率为3.93%。结论:总黄酮正交提取工艺的研究,结果比较可靠,含量测定方法简便、准确,可用于藤三七中总黄酮的含量测定。

不同炮制方法对藤三七珠芽总黄酮含量的影响

目的:通过对藤三七珠芽药材进行清蒸、酒蒸,并测定总黄酮含量,为充分利用藤三七珠芽而作出科学参考。方法:以芦丁为标准品,采用紫外分光光度法建立标准曲线Y=9.813X+0.028 7,测定并比较清蒸、酒蒸以及生品藤三七中总黄酮含量,得出藤三七珠芽的最佳使用方法。结果:其总黄酮测定芦丁浓度在0.017 74~0.070 98 mg/mL时,吸光度A在0.2~0.8之间,测得生品藤三七珠芽总黄酮含量为3.99%,蒸制品含量为2.25%,酒蒸含量为2.55%。结论:本研究建立的测定总黄酮的方法专属性强,适用于藤三七珠芽的总黄酮质量测定。生品的总黄酮含量最高,其次是酒蒸,蒸制含量最低。