Antagonistic Effect of Russula virescens(Schaeff.) Fr.against Bortrytis cinerea

[Objective] The paper was to study the antagonistic effect of Russula virescens （Schaeff.） Ft. against Bortrytis cinerea. [Method] Using the confrontation culture method, the antagonistic effect of mycelium, fermentation broth and mycelial extract of R. virescens against B. cinerea was studied. [Result] The antagonistic ef- fect of R. vivesscens against B. cinerea was mainly manifested as hyperparasitism of mycelium and inhibition effect of metabolites. The inhibition rate of confrontation growth was between 46.9% and 52.3%, and hyperparasitism phenomenon was obvi- ous. The fermentation broth of R. virescens cultured by PDB medium had the strongest growth inhibition effect, and the growth inhibition rate against B. cinerea was the highest of 61.59%. The thermal stability of fermentation broth was good, the mycelial extract of R. virescens had no inhibition effect against the growth of B. cinerea. [Conclusion] The study provided theoretical basis for subsequent differentiation and drug sensitivity of B. cinerea.

Mechanism of antagonistic effects of Andrographis paniculata methanolic extract against Staphylococcus aureus

Objective: To investigate the effects of Andrographis paniculata(Burm.f.) Wall. Ex Nees(A. paniculata) on expressions and activities of catalase, superoxide dismutase and alkylhydroperoxide reductase C in Staphylococcus aureus(S. aureus) with respect to its survival in vitro. Methods: Antioxidative property of methanolic leaves extract of A. paniculata(0.06 mg/m L). Minimum inhibitory concentration(MIC) was determined by its ability to reduce hydrogen peroxide(H_2O_2) toxicity against S. aureus ATCC 25923(3.8×10~8 cfu/m L). Effects of the extract on expressions of kat A(encoding catalase), sod A and sod M [encoding superoxide dismutases(SODs)], and ahp C [encoding alkylhydroperoxide reductase C(AhpC)] in S. aureuswere determined by RT-q PCR and corresponding enzyme activity assays were performed. Nitroblue tetrazolium reduction(NBT) assay was performed to determine effects of the extract on intracellular and extracellular levels of O_2-in S. aureus. Results: Cells challenged with 7.5 mmol/L H_2O_2 showed 0% survival in 30 min whereas 25% survived after treatment with the extract and H_2O_2. Cells that were treated with the extract alone had 43% survival in the same exposure period. Expressions of sod A and sod M genes in extract-treated cells were lowered 0.8-fold and 0.7-fold, respectively with decrease in total SOD activity of 26.8 U compared to untreated cells, 32.4 U(P<0.05). In contrast, extract-treated S. aureus cells showed 3.3-fold increase in kat A expression with corresponding increase in catalase activity of 1.828 U compared to untreated cells which was 1.248 U,(P<0.05). More profoundly, ahp C expression was increased 61-fold in extract-treated cells,(P<0.05) with corresponding increase in Ahp C activity of 0.018 U compared to untreated cells, 0.012U,(P<0.05). Extract-treated cells had significantly lower intra-and extracellular O_2-levels with absorbance readings(A_(575nm)) of 0.340 and 0.524 compared to untreated cells which were 0.516 and 0.928(P<0.05), respectively. Conclusions: Taken together these results suggest that the low MIC of A. paniculatamethanolic leaves extract(0.06 mg/m L) reduce H_2O_2 toxicity and more importantly, was in itself effectively inhibitory against S. aureus. Further, our observations suggest that a probable mode of its inhibitory mechanism against S. aureus is by reducing total SOD activity through downregulation of sod A and sod M expressions.

Screening,identification and antagonistic effect of antagonistic bacteria JTFM1001 against aflatoxin contamination in corn

Aflatoxin is a strong carcinogenic and toxic fungal toxin produced by Aspergillus flavus and other Aspergillus species,and can seriously threaten the health of consumers,thus becoming a global concern.Corn,as an important oil and economic crop,is highly susceptible contaminated by aflatoxin.In this study,antagonistic bacteria with strong inhibitory effect on aflatoxin were screened to provide support for the treatment aflatoxin contamination control in corn.Ten strains which have strong antagonistic effects against A.flavus were isolated from healthy corn from different corn producing areas in China.Among them,the antagonistic bacteria JTFM1001 through corn kernels in vivo and field experiment,the inhibition effect of aflatoxin contamination reached above 70%and 55%,respectively.And the strain was identified as Bacillus subtilis based on its morphological,physiological and biochemical characteristics and phylogenetic analysis of 16S rDNA.In addition,our data showed that it can colonize in the rhizosphere and survive for a long time,forming the dominant flora,with broad application prospect.Finally,we were surprised to find that the antibacterial metabolites secreted by the antagonistic bacteria was one of the mechanisms of its inhibition of A.flavus and aflatoxin.This will provide us with new ideas and perspectives on the effective prevention and control of aflatoxin contamination in corn and corn oil.

Protective Effects of Calcium Antagonists on Cadmium-induced Toxicity in Rats

Protective effects of calcium antagonists, chlorpromazine (CPZ) and nimodepine (NI-MO), on cadmium-induced toxicity were investigated. After giving CdCl2 (0. 44mg Cd/kg,ip), CPZ (5mg/kg, ip) or NIMO (8mg/kg, po) were administered every day to Sprague-Dawley (S. D. ) rats for a week. Then, urinary N- acetyl-β-D- glucosaminidase (NAG ), uri -nary cadmium and bloocl cadmium were measured. The accumulation of cadmium in the kid-ney cortex, content of renal calmodulin, hemoglobin and the ultrastructural damage of proxi-mal convoluted tubules of rats were examined three weeks after the last administration. Re-sults indicated that the calcium antagonists partly protected against toxic effects induced bycadmium in different manners. These data provide further evidence for the new hypothesisthat the cross effect of cadmium and calcium in calmodulin regulated systems may be responsi-ble for the mechanism of cadmium intoxication. 'The results suggested that the calcium antag-onists could be a new and promising approach in the therapy of heavy metaLinduced diseases

Determination of Growth-promoting and Antagonistic Action of Endophytic Bacteria Strains Itb57 and Itb295 of Tobacco

[ Objective ] The paper was to study the growth-promoting and antagonistic action of endophytic bacteria strains Itb57 and Itb295 of tobacco to explore their functions in biological control. [ Method] The growth-promoting effects of bacterial suspension ~ff endophytic bacteria Itb57 and Itb295 on tobacco seedling un- der different treatment modes were studied using potting method in greenhouse. The antagonistic action of bacterial suspension of endophytic bacteria Itb57 and Itb295 on Phytophthora nicotianae, Alternaria alternata and Botrytis cinerea were measured by duel culture method. [ Result] Bacterial suspensions of enduphytic bacteria Itb57 and Itb295 had certain growth-promoting effects on tobacco seedling, which could significantly increase the fresh weight and dry weight in aerial part; the growth-promoting effect of soaking ＋ spraying and irrigating treatment was the best. Itb57 strain had good antagonistic action against P. nicotianae. A. alterna- ta and B. cinerea, while Itb295 strain only had good antifungal effect against P. nicotianae. [ Conclusion] The results provided basis for the study and application of tobacco endophytic bacteria strains Itb57 and Itb295 in biocontrol of tobacco diseases.

Joint effects of microwave and chromium trioxide on root tip cells of Vicia faba

The mutagemc effects of microwave and chromium trioxide （CrO3） on Vicia faba root tip were studied. Micronucleus assay and chromosomal aberration assay were used to determine the mitotic index, the micronucleus frequency and chromosomal aberration frequency of Vicia faba root tip cells induced by microwave and CrO3. The results showed that the micronucleus frequency decreased, and that the mitotic index and chromosomal aberration frequency showed linear dose responses to CrO3, in treatment of microwave for 5 s. In microwave of 25 s, the mitotic index decreased, the micronucleus frequency and chromosomal aberration frequency increased with increase of CrO3 concentration. We concluded that microwave and CrO3 had antagonistic effect on the mitotic index of Vicia faba root tip cells, but had synergetic effect on micronucleus frequency and chromosomal aberration frequency of Viciafaba root tip cells.

Isolation,Screening and Identification of Antagonistic Actinomycetes against Ustilago scitaminea Syd

[Objectives] This study was conducted to obtain actinomycetes strains having antagonistic effect on Ustilago scitaminea Syd.[Methods] At first, actinomycetes strains were isolated from 22 soil samples in Hainan sugarcane regions. Then, antagonistic actinomycetes against U. scitaminea were screened by confrontation culture. Finally, the taxonomic status of antagonistic actinomycetes was determined using 16S rDNA.[Results] From the 22 samples, 984 actinomycetes strains were isolated. From all the isolated strains, 23 antagonistic actinomycetes strains were obtained through primary screening, and one strains with better antagonistic effect was then obtained through secondary screening, and designated FAS. 16S rDNA identification showed that strain FAS shared 99% sequence similarity with Streptomyces cealestis US24. A phylogenetic tree was built with MAGE 7.0 software, and the results showed that strain FAS had the shortest genetic distance with S. caelestis US24. Therefore, the actinomycetes FAS was determined as S. caelestis .[Conclusions] This study provides a new biocontrol method for the biological control of sugarcane smut, thereby ensuring sustainable development of sugarcane industry and sugar industry.

Electrochemical Study of the Synergistic Effect of Two Copper Corrosion Inhibitors, Nicotinic Acid (NAC) and Nicotinamide (NAM) in Two Different Media

Inhibitive properties of NAC and NAM as well as their mixture have been investigated in two different corrosive media through potentiodynamic polarization and electrochemical impedance spectroscopy at 298 K. These electrochemical measurements indicate that the inhibition efficiency increases with the concentration for each of the inhibitors used separately as well as for their mixture. In both cases, the combination behaves as mixed type inhibitor with a great cathodic tendency. NaCl 3.5% highlights an antagonist effect of NAM which reduces the effectiveness of NAC whereas HNO3 1M shows a synergistic effect between them.

Study on the Degradation and Synergistic /antagonistic Antioxidizing Mechanism of Phenolic /aminic Antioxidants and Their Combinations

Oxidation stability of lubricating oil is critical for the smooth operation of industrial equipment such as turbines.Sludge formation is a major issue in turbine operation.Currently,the turbine oil industry employs antioxidants,especially,the combination of aminic and phenolic antioxidants,as the key additives to reduce sludge formation.Aromatic amines,primarily PANA,are considered as the most effective antioxidants in terms of oxidation induction time(OIT)measured by the RPVOT test.However,PANA is also a sludge former.Phenolic antioxidants are normally used in combination with the aminic antioxidants.It is widely believed that there exists a synergistic effect between the phenolic and aminic antioxidants.Regarding the interaction mechanism between the two types of antioxidants,it is generally accepted that phenolics act as a hydrogen donor to help regenerate the consumed aminic antioxidants.This mechanism can only explain some of the phenomena observed in field practice,but there are many other questions remaining unanswered including the antagonistic effect between PANA and phenolics.In this study,we used HPLC,FTIR and LC-MS to monitor the degradation products of the oil systems that were formulated with various aminic and phenolic antioxidants.For the APANA alone system,we identified the formation of dimers and trimers of APANA.For the PANA alone system,we identified the formation of dimers and tetramers.The dimers,trimers,and tetramers of PANA have very limited solubility.These findings therefore help explain why PANA is a sludge former.The oligomerizations(dimerization,trimerization,etc.)regenerate the N-H functionality,thus explaining why the PANA as well as other amines are very effective antioxidants.For the PANA/phenolic system,the oligomers of PANA were greatly reduced.Instead,we identified species that were formed by the recombination of the PANA radicals,or the PANA oligomeric radicals with the phenolic radicals.This,to our knowledge,has not been reported so far.The recombination of aminyl radicals and the phenolic radicals decreases the capability of regenerating the N-H functionality,which helps explain the antagonistic effect observed in the PANA/phenolic system.It also suggests that the phenolic antioxidants are very important in suppressing the sludge formation in the turbine oil system because the recombination products of PANA and phenolics have much better solubility than the PANA oligomers.

再流转中土地经营权的登记效力规则研究

再流转中的两种土地经营权在登记能力、登记效力上采区分原则。再流转阶段,基于土地承包经营权的土地经营权承继土地承包经营权的流转规则而采登记对抗效力,“未经登记,不得对抗善意第三人”应解释为未登记可以对抗恶意第三人以及能对抗善意第三人的必要条件为已登记,登记对抗效力表现为登记公示相较于其他公示方式的优先性与相对公信力,占有使用及向发包人备案作为认定第三人善意的实质调查内容在司法实践中发挥作用。以其他方式承包取得的土地经营权再流转须“经依法登记取得权属证书”为管理性规定,同时暗含登记连续性的目的,采登记生效效力的形式正当性在于此类土地经营权再流转的物权变动在实证法上的相对独立性,进而适用不动产物权公示的原则性规定,实质正当性在于通过市场机制高效利用农村“四荒地”土地资源的价值取向。另外,可结合此类土地经营权再流转的特殊规制目的适用善意取得制度。

白细胞介素1受体颉颃剂抑制脂多糖促奶牛外周血单个核细胞氧化应激损伤作用的研究

试验以脂多糖(LPS)为刺激源,以细胞活力、抗氧化指标和炎症因子为判断指标,探讨白细胞介素1受体颉颃剂(IL-1Ra)通过抑制白细胞介素1β(IL-1β)的活性,对LPS诱导外周血单个核细胞(Peripheral blood mononuclear cells,PBMCs)氧化损伤的缓解作用。试验采用单因子完全随机设计,PBMCs被随机分为7个组(每组6个重复),分别给予不同的处理:第1组是阴性对照组(Neg组),完全培养基培养30 h;第2组损伤组(Dam组),是在完全培养基中培养6 h后,再经10μg/mL的LPS工作液培养24 h;第3至7组(R0.25、R0.5、R1、R5组和R10组)细胞分别经浓度为0.25、0.5、1、5、10 ng/mL的IL-1Ra培养6 h,接着经10μg/mL的LPS工作液培养24 h。结果表明:与Neg组相比,Dam组的细胞活力、抗氧化相关酶[包括总抗氧化能力(T-AOC)以及总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)和硫氧还蛋白还原酶(TrxR)]的活性显著降低,丙二醛(MDA)浓度、炎症因子白细胞介素-6(IL-6)和IL-1β含量以及诱导型一氧化氮合酶(iNOS)活性、一氧化氮(NO)含量均显著升高(P≤0.05)。与Dam组相比,R1组显著逆转了氧化损伤引起的上述抗氧化活性的降低和炎症因子浓度的升高,其他IL-1Ra处理组对上述指标的逆转效果不同程度地低于R1组(P≤0.05)。上述结果说明,LPS通过诱发PBMCs产生大量IL-1β进而导致细胞氧化损伤,IL-1Ra剂量依赖性地缓解了LPS引起的氧化损伤,添加剂量以1 ng/mL为宜。

Electronic Structure of some A3 Adenosine-Receptor Antagonist——A Structure Activity Relationship

DFT quantum chemical computations have been carried out at the B3LYP/6-31G (d) level. Full geometry optimization has been performed and equilibrium geometries for a new series of phenyl thiazoles have been located. Ground state electronic properties, charge density distributions, dipole moments and its components have been calculated and reported. Effect of substituents on the geometry and on the polarization of the studied series of compounds are analyzed and discussed. Some structural features have been pinpointed to underline the affinity and selectivity of the studied compounds as adenosine A3-receptor antagonists. Results of the present work indicate that activity towards A3 receptor sites is directly correlated with both of the polarity and the co-planarity of the thiazole.

Effects of aminoguanidine on nitric oxide production induced by inflammatory cytokines and endotoxin in cultured rat hepatocytes

AIM: To study the effects of aminoguanidine (AG) and two L-arginine analogues N(omega)-nitro-L-arginine methyl ester (L-NAME) and N(omega)-nitro-L-arginine (L-NNA) on nitric oxide (NO) production induced by cytokines (TNF-alpha, IL-1 beta, and IFN-gamma) and bacterial lipopolysaccharide (LPS) mixture (CM) in the cultured rat hepatocytes, and examine their mechanisms action. METHODS: Rat hepatocytes were incubated with AG, L-NAME, L-NNA, Actinomycin D (ActD) and dexamethasone in a medium containing CM (LPS plus TNF-alpha, IL-1 beta, and IFN-gamma) for 24h. NO production in the cultured supernatant was measured with the Griess reaction. Intracellular cGMP level was detected with radioimmunoassy. RESULTS: NO production was markedly blocked by AG and L-NAME in a dose-dependent manner under inflammatory stimuli condition triggered by CM in vitro. The rate of the maximum inhibitory effects of L-NAME (38.9%) was less potent than that obtained with AG(53.7%, P 【 0.05). There was no significant difference between the inhibitory effects of AG and two L-arginine analogues on intracellular cGMP accumulation in rat cultured hepatocytes. Non-specific NOS expression inhibitor dexamethasone (DEX)and iNOS mRNA transcriptional inhibitor ActD also significantly inhibited CM-induced NO production. AG(0.1 mmol x L(-1)) and ActD (0.2 ng x L(-1)) were equipotent in decreasing NO production induced by inflammatory stimuli in vitro, and both effects were more potent than that induced by non-selectivity NOS activity inhibitor L-NAME (0.1 mmol x L(-1)) under similar stimuli conditions (P【0.01). CONCLUSION: AG is a potent selective inhibitor of inducible isoform of NOS,and the mechanism of action may be not only competitive inhibition in the substrate level, but also the gene expression level in rat hepatocytes.

植物根际促生菌F13的筛选、鉴定及对豆角促生、抗病的效果

为获得具有药肥双效的多功能植物根际促生菌,从葡萄根际土壤中分离出同时具有溶磷解钾功能的菌株F13。该菌通过形态学观察、生理生化检测和16S rDNA比对,鉴定为铜绿假单胞菌(Pseudomonas aeruginosa)。经平板对峙法测定菌株F13对8种植物病原菌的抑制率为63.26%~81.53%。田间应用中发现,菌株F13发酵液灌根处理对豆角(Vigna unguiculata subsp.sesquipedalis(L.)Verdc.)生长具有显著的促进作用,其中实验组在株高、叶片数、茎粗方面分别高于对照组26.7%、24.4%、11.9%,产量高于对照组39.68%;在植株吸收磷、钾元素方面分别高于对照组11.5%、46.8%;在豆角果实中维生素C、可溶性蛋白含量方面分别高于对照组22.15%、55.55%。此外,菌株F13对豆角白粉病的相对防效达71.92%。因此,菌株F13是一株具有巨大应用潜力的药肥双效植物根际促生菌。

解淀粉芽孢杆菌YK3对沃柑溃疡病的防效及叶际细菌群落相关性的影响

【目的】探究解淀粉芽孢杆菌YK3对沃柑溃疡病的防治效果及对沃柑叶际细菌网络关系的影响,为微生物防治沃柑溃疡病提供实际参考。【方法】采用平板抑菌法分离沃柑溃疡病的拮抗菌,离体生测和盆栽实验研究拮抗菌对沃柑溃疡病的防治效果,并通过高通量测序技术研究接种拮抗菌对沃柑叶际细菌群落组成、相对丰度以及细菌互作关系网络的影响。【结果】分离获得一株发酵液和发酵过滤液均对病原菌有强烈抑制作用的解淀粉芽孢杆菌YK3,该菌株在以有机氮作为氮源时才会产生抑菌物质,不能与铜类农药同时使用。生防测试实验结果表明,仅接种病原菌的病情指数为96.67%,同时接种病原菌和YK3的病情指数为33.33%,YK3对沃柑溃疡病的相对防治效果为65.56%。YK3可以降低沃柑叶际Xanthomonas的相对丰度。仅接种YK3的沃柑叶际细菌群落正相关关系最复杂,其次为同时接种病原菌和YK3处理,接种病原菌处理的沃柑叶际细菌群落正相关关系最弱。同时接种病原菌和YK3处理中Xanthomonas、Bacillus与沃柑叶际土著细菌群落均为负相关关系,但是Xanthomonas和Bacillus与沃柑叶际土著细菌群落的关系网不存在重叠,Xanthomonas和Bacillus独自面对不同的土著细菌的竞争作用。【结论】解淀粉芽孢杆菌YK3对沃柑溃疡病有较好的防治效果,会改变沃柑叶际细菌多样性和结构,使沃柑叶际土著细菌群落的正相关性网络增强,负相关性网络减弱,互作关系网络更密集。

砖红镰刀菌菌株Pa2对草莓黑斑病菌的抑制活性分析

【目的】探究内生真菌砖红镰刀菌(Fusarium lateritium)菌株Pa2对草莓黑斑病菌(Alternaria alternata)的抑制效果,为开发草莓黑斑病生防菌剂奠定理论基础。【方法】以草莓黑斑病为防治对象,通过菌丝生长速率法、孢子萌发法以及SYTOX绿菁染色法分析菌株Pa2对草莓黑斑病菌的菌丝生长、孢子萌发、细胞膜通透性的影响,通过离体果实与叶片测定砖红镰刀菌菌株Pa2对草莓黑斑病菌的防治效果,初步探讨砖红镰刀菌Pa2对草莓黑斑病的抑制活性与防效。【结果】平板对峙试验表明,菌株Pa2对草莓黑斑病菌生长抑制效果达80.96%。菌株Pa2上清液不仅可以破坏菌丝细胞膜,而且对病菌菌丝生长以及孢子萌发的抑制能力随上清液浓度的升高而增强。同时,菌株Pa2能够显著抑制草莓黑斑病菌在果实与叶片上的侵染能力。【结论】砖红镰刀菌菌株Pa2对草莓黑斑病菌具有很好的抑制作用,在草莓黑斑病的生物防治方面具有潜在的应用价值。

干旱荒漠区三种典型灌丛植物凋落物混合分解效应研究

为探究干旱荒漠区混合凋落物的分解特征,本研究以红砂(Reaumuria soongarica(Pall.)Maxim)、泡泡刺(Nitraria sphaerocarpa Maxim)和珍珠(Salsola passerina Bunge)为研究对象,分析了单一和混合凋落物质量损失率和养分残留率的变化,揭示了混合凋落物分解的加性与非加性效应。结果表明:各凋落物质量损失率随分解时间延长呈增加趋势;各凋落物C和N残留率随分解时间推移为降低趋势,说明C和N养分元素呈释放模式;混合凋落物质量损失率表现为加性效应,C残留率在分解前期为加性效应,分解末期为拮抗的非加性效应,N残留率在分解前期为拮抗和协同的非加性效应,分解末期为加性效应;凋落物混合后,较高的物种丰富度会导致养分残留率的非加性效应增强。综上,混合凋落物分解中养分变化的混合效应因分解时间而不同,混合凋落物物种丰富度会影响养分元素的混合效应,能改善干旱荒漠生态系统的养分循环。

黄瓜霜霉病生防菌ZF515分离鉴定及防效试验

霜霉病是黄瓜栽培中最常见的一种具有毁灭性的病害,常造成巨大的经济损失。生物防治是一种能够有效防治作物病害且环境友好的重要措施。为筛选出有效防治黄瓜霜霉病的生防菌株,从黄瓜根际土样中分离获得121株芽胞杆菌。采用盆栽试验方法筛选获得8株对黄瓜霜霉病具有良好防效的生防菌。使用这8株生防菌在不同地区大棚内进行田间试验,确定生防菌株ZF515对黄瓜霜霉病具有稳定防效,防效高达71.47%。根据形态特征、生理生化特性以及多基因系统发育树分析确定ZF515菌株为贝莱斯芽胞杆菌Bacillus velezensis。采用平板对峙法明确ZF515菌株对8种不同病原真菌均具有抑制作用。综上所述,菌株ZF515对黄瓜霜霉病具有很好的生防潜力,是一株具有开发利用价值的生防菌株。

香蕉抗枯萎病株系内生菌的分离鉴定及抑菌促生效果

【目的】筛选和鉴定从威廉斯香蕉抗枯萎病株系根、球茎及假茎分离得到的可抑制香蕉枯萎病菌(Fusarium oxysporum f.sp.cubense,Foc)内生菌,研究其抑菌促生效果。【方法】通过16S rRNA基因序列测定和生理生化试验,对香蕉抗枯萎病株系内生菌进行种类鉴定和系统发育分析;通过平板对峙试验检测各菌株的抑菌能力,探究拮抗菌分泌抑菌相关活性物质的种类;采用盆栽试验对香蕉抗枯萎病株系内生菌进行生防功能和促生功能的分析,筛选出生防性能优良且兼具促生效果的内生菌,并在大田试验中验证。【结果】分离获得13株代表菌株,其中8株对枯萎病1号生理小种(Foc 1)具有抑菌活性,7株对枯萎病4号生理小种(Foc 4)具有抑菌活性;综合盆栽试验和大田试验中代表菌株的防治效果及促生能力,筛选出贝莱斯芽孢杆菌XG1028、变栖克雷伯氏菌XQJ0301和暹罗芽孢杆菌XG0103共3株具备高抑菌活性以及促生能力的生防菌株。【结论】本研究筛选出的3株菌株丰富了抗香蕉枯萎病微生物种质资源,为防治香蕉枯萎病寻找安全、环保的新型生物防治剂提供参考。

非肽类促性腺激素释放激素拮抗剂在子宫肌瘤治疗中的应用研究进展

非肽类促性腺激素释放激素拮抗剂(gonadotropin-releasing hormone antagonist,GnRH-antagonist)作为GnRH受体的一种高效、选择性拮抗剂,其口服制剂(Elagolix,Relugolix及Linzagolix)已在国外获批上市,在用于子宫肌瘤缩小肌瘤体积、控制月经过多等方面展示了一定疗效,适当配伍反加治疗可以提高药物安全性与患者依从性,相比其他激素类制剂,口服GnRH拮抗剂在子宫肌瘤治疗中展示了良好的应用前景。