How plant density affects maize spike differentiation, kernel set, and grain yield formation in Northeast China?

A two-year field experiment was conducted to evaluate the effects of plant density on tassel and ear differentiation, anthesissilking interval(ASI), and grain yield formation of two types of modern maize hybrids(Zhongdan 909(ZD909) as tolerant hybrid to crowding stress, Jidan 209(JD209) and Neidan 4(ND4) as intolerant hybrids to crowding stress) in Northeast China. Plant densities of 4.50×104(D1), 6.75×104(D2), 9.00×104(D3), 11.25×104(D4), and 13.50×104(D5) plants ha-1had no significant effects on initial time of tassel and ear differentiation of maize. Instead, higher plant density delayed the tassel and ear development during floret differentiation and sexual organ formation stage, subsequently resulting in ASI increments at the rate of 1.2–2.9 days on average for ZD909 in 2013–2014, 0.7–4.2 days for JD209 in 2013, and 0.5–3.7 days for ND4 in 2014, respectively, under the treatments of D2, D3, D4, and D5 compared to that under the D1 treatment. Total florets, silking florets, and silking rates of ear showed slightly decrease trends with the plant density increasing, whereas the normal kernels seriously decreased at the rate of 11.0–44.9% on average for ZD909 in 2013–2014, 2.0–32.6% for JD209 in 2013, and 9.7–28.3% for ND4 in 2014 with the plant density increased compared to that under the D1 treatment due to increased florets abortive rates. It was also observed that 100-kernel weight of ZD909 showed less decrease trend compared that of JD209 and ND4 along with the plant densities increase. As a consequence, ZD909 gained its highest grain yield by 13.7 t ha-1on average at the plant density of 9.00×104 plants ha-1, whereas JD209 and ND4 reached their highest grain yields by 11.7 and 10.2 t ha-1at the plant density of 6.75×104 plants ha-1, respectively. Our experiment demonstrated that hybrids with lower ASI, higher kernel number potential per ear, and relative constant 100-kernel weight(e.g., ZD909) could achieve higher yield under dense planting in high latitude area(e.g., Northeast China).

Breeding for Drought Tolerance in Maize (Zea mays L.)

Drought, like many other environmental stresses, has adverse effects on crop yield including maize (Zea mays L.). Low water availability is one of the major causes for maize yield reductions affecting the majority of the farmed regions around the world. Therefore, the development of drought-tolerant lines becomes increasingly more important. In maize, a major effect of water stress is a delay in silking, resulting in an increase in the anthesis-silking interval, which is an important cause of yield failures. Diverse strategies are used by breeding programs to improve drought tolerance. Conventional breeding has improved the drought tolerance of temperate maize hybrids and the use of managed drought environments, accurate phenotyping, and the identification and deployment of secondary traits has been effective in improving the drought tolerance of tropical maize populations and hybrids as well. The contribution of molecular biology will be potential to identify key genes involved in metabolic pathways related to the stress response. Functional genomics, reverse and forward genetics, and comparative genomics are all being deployed with a view to achieving these goals. However, a multidisciplinary approach, which ties together breeding, physiology and molecular genetics, can bring a synergistic understanding to the response of maize to water deficit and improve the breeding efficiency.