[Objective] This research aimed to optimize continuously the highly efficient regeneration system of Anthurium andraeanum. [Method] The leaves and petioles of four A. andraeanum varieties were used as explants to inve...[Objective] This research aimed to optimize continuously the highly efficient regeneration system of Anthurium andraeanum. [Method] The leaves and petioles of four A. andraeanum varieties were used as explants to investigate the differences in primary callus induction among different A. andraeanum varieties. [Result]The callus formation capacity of SAM and SST was stronger than that of SDM and SHG. Among the four varieties, the leaf regeneration capacity of SAM, SDM and SHG was stronger than the corresponding petiole regeneration capacity. However,the petiole regeneration capacity of SST was stronger. The optimum medium for petiole callus induction of SST was 1/2 MS ＋ TDZ 4.0 mg/L ＋ 2, 4-D 0.2 mg/L with induction rate of 87.5%; the optimum medium for leaf callus induction of SAM was 1/2 MS ＋ TDZ 2.0 mg/L ＋ 2, 4-D 0.2 mg/L with induction rate more than90%; the optimum medium for leaf callus induction of SDM and SHG was all 1/2MS ＋ ZT 2.0 mg/L ＋ 2, 4-D 0.2 mg/L with induction rates of 59.34% and 79.63%,respectively. [Conclusion] In addition to variety differences, the differences in differentiation ability among different types of calluses should be also taken into account in the establishment and optimization of tissue culture and rapid propagation technology system of A. andraeanum.展开更多
This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture te...This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture technique, the conditions for callus induction, protocorm-like body （PLB） formation and plant regeneration from leaf explants and petiole of A. andraeanum, such as basal medium and plant growth regulator, were investigated. Totipotent callus was induced on a 1/2-strength MS medium containing 0.90 μmol L^-1 2,4-dichlorophenoxyacetic acid （2,4-D） and 8.88μmol L^-1 N6-benzyladenine （BA）. The callus exhibited complete hormone autonomy for growth and differentiation of PLBs. This callus proliferated well and was maintained by subculturing on 1/2 MS medium containing 0.90 μmol L^-1 2,4-D and 4.44 μmol L^-1 BA. On average, 8 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the 1/2 MS medium with 4.44 μmol L^-1 BA after 8 wk of culture. The regenerated PLBs formed shoots and roots on 1/2 MS medium. After 24 wk of culture on these medium, well-developed plantlets for potting were produced. An efficient micropropagation method was established for indirect PLB formation and plant regeneration from leaf and petiole ofA. andraeanum.展开更多
以红掌3个不同部位的材料(叶片、叶柄、茎段)为接种外植体,对红掌的离体培养技术进行了初步的研究。结果表明,不同部位对愈伤组织诱导差异显著,其中叶柄诱导率最高,达到86.7%,为最佳取材部位。诱导愈伤组织培养基以M S+6-BA 2.0 m g.L-1...以红掌3个不同部位的材料(叶片、叶柄、茎段)为接种外植体,对红掌的离体培养技术进行了初步的研究。结果表明,不同部位对愈伤组织诱导差异显著,其中叶柄诱导率最高,达到86.7%,为最佳取材部位。诱导愈伤组织培养基以M S+6-BA 2.0 m g.L-1+2,4-D 0.2 m g.L-1为最好;M S+BA 2.0 m g.L-1+NAA 0.25 m g.-L 1对不定芽分化效果良好。展开更多
利用从观赏植物上截获的相似穿孔线虫Radopholus similis 6个种群,研究在胡萝卜愈伤组织上长期培养繁殖和在红掌Anthurium andraeanum上接种繁殖复壮后,相似穿孔线虫耐低温能力的变化.将经2种方式培养繁殖的相似穿孔线虫接种在胡萝卜愈...利用从观赏植物上截获的相似穿孔线虫Radopholus similis 6个种群,研究在胡萝卜愈伤组织上长期培养繁殖和在红掌Anthurium andraeanum上接种繁殖复壮后,相似穿孔线虫耐低温能力的变化.将经2种方式培养繁殖的相似穿孔线虫接种在胡萝卜愈伤组织上,测定其耐低温能力.结果表明,在胡萝卜愈伤组织上长期(6代)连续培养后,相似穿孔线虫的耐低温能力降低,在低温下的生存能力和繁殖能力明显降低;通过在自然寄主植物红掌根系上接种繁殖后,相似穿孔线虫在低温下的生存和繁殖能力又可得到不同程度的恢复,即耐低温能力得到复壮.胡萝卜愈伤组织上长期培养繁殖对相似穿孔线虫不同群体耐低温能力的影响不同,相似穿孔线虫不同种群在红掌上繁殖后耐低温能力的恢复程度也有所不同.展开更多
文摘[Objective] This research aimed to optimize continuously the highly efficient regeneration system of Anthurium andraeanum. [Method] The leaves and petioles of four A. andraeanum varieties were used as explants to investigate the differences in primary callus induction among different A. andraeanum varieties. [Result]The callus formation capacity of SAM and SST was stronger than that of SDM and SHG. Among the four varieties, the leaf regeneration capacity of SAM, SDM and SHG was stronger than the corresponding petiole regeneration capacity. However,the petiole regeneration capacity of SST was stronger. The optimum medium for petiole callus induction of SST was 1/2 MS ＋ TDZ 4.0 mg/L ＋ 2, 4-D 0.2 mg/L with induction rate of 87.5%; the optimum medium for leaf callus induction of SAM was 1/2 MS ＋ TDZ 2.0 mg/L ＋ 2, 4-D 0.2 mg/L with induction rate more than90%; the optimum medium for leaf callus induction of SDM and SHG was all 1/2MS ＋ ZT 2.0 mg/L ＋ 2, 4-D 0.2 mg/L with induction rates of 59.34% and 79.63%,respectively. [Conclusion] In addition to variety differences, the differences in differentiation ability among different types of calluses should be also taken into account in the establishment and optimization of tissue culture and rapid propagation technology system of A. andraeanum.
基金supported by the Natural Science Foundation of Guangdong Province (05300848)Fok Ying Tung Education Foundation (104031)the National Natural Science Foundation of China(30800758)
文摘This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture technique, the conditions for callus induction, protocorm-like body （PLB） formation and plant regeneration from leaf explants and petiole of A. andraeanum, such as basal medium and plant growth regulator, were investigated. Totipotent callus was induced on a 1/2-strength MS medium containing 0.90 μmol L^-1 2,4-dichlorophenoxyacetic acid （2,4-D） and 8.88μmol L^-1 N6-benzyladenine （BA）. The callus exhibited complete hormone autonomy for growth and differentiation of PLBs. This callus proliferated well and was maintained by subculturing on 1/2 MS medium containing 0.90 μmol L^-1 2,4-D and 4.44 μmol L^-1 BA. On average, 8 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the 1/2 MS medium with 4.44 μmol L^-1 BA after 8 wk of culture. The regenerated PLBs formed shoots and roots on 1/2 MS medium. After 24 wk of culture on these medium, well-developed plantlets for potting were produced. An efficient micropropagation method was established for indirect PLB formation and plant regeneration from leaf and petiole ofA. andraeanum.
文摘以红掌3个不同部位的材料(叶片、叶柄、茎段)为接种外植体,对红掌的离体培养技术进行了初步的研究。结果表明,不同部位对愈伤组织诱导差异显著,其中叶柄诱导率最高,达到86.7%,为最佳取材部位。诱导愈伤组织培养基以M S+6-BA 2.0 m g.L-1+2,4-D 0.2 m g.L-1为最好;M S+BA 2.0 m g.L-1+NAA 0.25 m g.-L 1对不定芽分化效果良好。
文摘利用从观赏植物上截获的相似穿孔线虫Radopholus similis 6个种群,研究在胡萝卜愈伤组织上长期培养繁殖和在红掌Anthurium andraeanum上接种繁殖复壮后,相似穿孔线虫耐低温能力的变化.将经2种方式培养繁殖的相似穿孔线虫接种在胡萝卜愈伤组织上,测定其耐低温能力.结果表明,在胡萝卜愈伤组织上长期(6代)连续培养后,相似穿孔线虫的耐低温能力降低,在低温下的生存能力和繁殖能力明显降低;通过在自然寄主植物红掌根系上接种繁殖后,相似穿孔线虫在低温下的生存和繁殖能力又可得到不同程度的恢复,即耐低温能力得到复壮.胡萝卜愈伤组织上长期培养繁殖对相似穿孔线虫不同群体耐低温能力的影响不同,相似穿孔线虫不同种群在红掌上繁殖后耐低温能力的恢复程度也有所不同.
