Production and Characterization of Recombinant Rat Non-Collagen Domain of α3 Chain of Type IV Collagen α3 (IV) NC1 Antigen

The glomerulonephritis disease is characterized by inflammation of glomeruli or small blood vessels in the kidney that causes kidney diseases. The reason of glomerulonephritis disease is to deposit the anti-GBM auto antibody in the glomerular basement membrane. The type IV collagen is the main component of glomerular basement membrane that has α3 chain of type (IV) collagen of non-collagenous domain which contains N-terminal 7S domain, a triple helical collagenous domain and C-terminal non-collagenous glomerular domain (NC1). The amino terminal of α3 (IV) NC1 that induces the Experimental Autoimmuno Glomerulonephritis (EAG) in rat model has been identified. The recombinant rat α3 (IV) NC1 antigen has nine amino acid spans that are consistent with antibody or T cell epitope that induces in EAG. The research is carried out on the recombinant rat α3 (IV) NC1 production, purification, quantification, and characterization. The circulation of anti-GBM antibody in glomerular basement membrane can be measured by the ELISA assay. In addition, the recombinant rat antigen is secreted in HEK293 cell supernatant that is purified by Anti-FLAG M2 monoclonal IgG antibody affinity column and characterized and quantified by SDS-PAGE gel electrophoresis and Western blotting techniques.

抗酪氨酸酶抗体和抗酪氨酸酶相关蛋白-1抗体检测与快速进展期白癜风疗效的相关性分析

目的探讨快速进展期白癜风口服复方中药联合激素和免疫调节剂的临床疗效与抗酪氨酸酶抗体(TYR IgG)和抗酪氨酸酶相关蛋白-1抗体(TRP-1 IgG)检测结果的关系。方法 68例门诊快速进展期白癜风患者治疗前后用酶联免疫吸附法检测血清TYR IgG和TRP-1 IgG的含量。治疗用复方倍他米松注射液1 m L深部肌肉注射,外用0.1%他克莫司软膏,口服复方中药祛白颗粒。结果白癜风快速进展期治疗有效率为89.71%(61/68)。治疗后TYR IgG和TRP-1 IgG含量较治疗前显著降低(P<0.05)。儿童组(≤14岁)治疗后TYR IgG和治疗前及治疗后TRP-1 IgG水平均低于>14岁组(P<0.05)。面颈部白癜风治疗效果最好。治疗方法不良反应很轻,不影响治疗过程。结论 TYR IgG和TRP-1 IgG检测结果与白癜风治疗效果密切相关,为白癜风的疗效提供了证据,具有指导继续治疗的应用价值。应用激素、免疫调节剂和复方中药治疗进展期白癜风有较好的疗效。

Role of M₃Muscarinic Acethylcholine Receptor Antibodies as a New Marker in Primary Sjögren Syndrome

Aims: This paper investigates the presence of M3 muscarinic acetylcholine receptor autoantibody present in the serum of patients with primary Sj?gren syndrome (pSS). Main methods: We detected the levels of M3mAChR peptide IgG, PGE2, IL-1β in serum of SS patients using the enzyme-linked immune sorbent assay (ELISA). To measure the quantity of nitrite/nitrate, we used Griess reagent system. Key findings: Titres of M3mAChR antibody in sera from SS patients are significantly enhanced compared to healthy subjects (control). The enhancement of these autoantibodies is accompanied by the increase of the levels of PGE2, IL-1β and nitrite/nitrate in serum. Under in vitro conditions, the synthetic human M3 peptide impaires the increment of M3mAChR antibody but not that of nati-Ro/SSA antibody. In positive anti-Ro/SSA antibody patients, the increment of M3mAChR peptide IgG and the measured pro-inflammatory substances is related. Significance: On this basis, anti M3mAChR peptide IgG can be said to act as a modulator of the immune system and to play a role in the host-chronic increment of proinflammatory substances in SS patients with positive Ro/SSA antibody. This association between the antibody and the pathogenesis of SS disease may result in useful predicting SS.

PLA2R、THSD7A与IgG亚型在特发性膜性肾病中的诊断价值

目的探讨特发性膜性肾病(idiopathic membranous nephropathy,IMN)患者血清抗磷脂酶A2受体(phospholipase A2 receptor,PLA2R)抗体水平、肾小球PLA2R、1型血小板反应蛋白7A域(thrombospondin type I domain containing 7A,THSD7A)、免疫球蛋白G(immunoglobulin G,IgG)亚型的表达及其在IMN中的诊断价值。方法选取2016年1月至2019年6月在雅安市人民医院肾内科经肾活检并确诊的IMN患者72例,以同期72例非IMN肾小球疾病患者为对照。采用酶联免疫吸附法检测血清抗PLA2R抗体滴度,免疫荧光法检测肾小球PLA2R、THSD7A及IgG亚型表达。采用受试者工作特征(ROC)曲线分析血清抗PLA2R抗体、肾小球PLA2R、THSD7A、IgG4诊断IMN的价值。结果血清抗PLA2R抗体、肾小球PLA2R、IgG4、THSD7A诊断IMN的灵敏度分别为61.11%、80.56%、97.22%、8.33%,特异度分别为97.22%、100.00%、97.22%、100.00%。血清抗PLA2R抗体和肾小球PLA2R任一指标阳性即诊断IMN的敏感性为83.33%;肾小球PLA2R、THSD7A和IgG4中任一指标阳性即诊断IMN的敏感性为97.22%。结论血清抗PLA2R抗体、肾小球PLA2R、THSD7A及IgG4亚型对于IMN诊断具有较高的临床价值。血清抗PLA2R抗体及肾小球PLA2R抗原的联合检测,肾小球PLA2R、THSD7A与IgG4的联合检测可以增加IMN诊断的敏感性。

白癜风患者血清中抗酪氨酸酶IgG抗体及抗酪氨酸酶相关蛋白1IgG抗体的检测

目的探讨白癜风患者血清中抗酪氨酸酶IgG抗体（TYRIgG）和抗酪氨酸酶相关蛋白1IgG抗体（TRP-1IgG）与白癜风的关系。方法用酶联免疫吸附测定检测260例白癜风患者和50例健康对照血清中TYR1gG和TRP-1IgG的含量。结果白癜风组TYRIgG和（或）TRP-1IgG阳性率57．31％（149／260），TYRIgG阳性率37．3％（97／260），TRP-1IgG阳性率33．5％（87／260），均明显高于对照组（r=25．441、21．630，P〈0．01）。TYRIgG阳性与TRP-1IgG阳性不相关（r=-0．032，P〉0．05）。白癜风组TYRIgG阳性率男女间差异无统计学意义（P〉0．05），但TRP-1IgG阳性率女性高于男性（r=5．811，P〈0．05）。白癜风TYRIgG阳性率≤20岁（35．9％）与〉20岁（38.3％）比较，差异无统计学意义（P〉0．05），而TRP-1IgG阳性率≤20岁组明显高于〉20岁组（x^2=6．498，P〈0．05）。结论检测TYRIgG和TRP-1IgG可为白癜风的诊断和治疗提供一定依据。

多灶性运动神经病的初步研究

目的对６例诊断为多灶性运动神经病（ＭＭＮ）的病例进行研究，并就一些相关问题进行讨论。方法采用ＣｏｕｎｔｅｒｐｏｉｎｔＭＫⅡ肌电图仪进行电生理研究，ＥＬＩＳＡ法测血及脑脊液中抗神经节苷脂ＧＭ１抗体，５例病人行腓肠神经活检。结果６例病人中５例为上肢起病，上肢受累为主，远端重。５例有感觉症状，但仅２例查体有轻度感觉缺失。均表现为无力、肌萎缩和腱反射减弱或消失。所有病人都有运动传导阻滞。４例病人血抗ＧＭ１抗体滴度升高。病理示脱鞘及髓鞘再生。结论结果表明ＭＭＮ为一高度特异的脱鞘性周围神经病，电生理以运动传导阻滞为特点。多数病人对免疫治疗有效。此研究与国外报道一致。

galE变异株及亲代株空肠弯曲菌诱导动物外周神经损伤的对比研究

目的探索空肠弯曲菌(Campylobactejejuni,CJ)致外周神经损伤的关键结构,为CJ感染相关格林巴利综合征的分子模拟理论提供动物实验证据。方法将32只豚鼠随机分为亲代株组(10只)、变异株组(10只)、对照组(6只)、PBS组(6只),分别用亲代株及弗氏佐剂、galE变异株及弗氏佐剂、PBS及弗氏佐剂、单纯PBS进行全身免疫;动态检测血清细胞壁脂寡糖(LOS)IgG及神经节苷脂GM1IgG抗体水平;检查坐骨神经病理改变,包括单纤维分离、半薄切片光镜及电镜检查。结果①免疫后变异株组及亲代株组LOSIgG抗体水平均明显增高,两组间差异无统计学意义;②免疫后14、28d,亲代株组GM1IgG抗体滴度(0.661±0.290,0.984±0.025)明显高于变异株组(0.193±0.078,0.180±0.063),差异均有统计学意义;③变异株组坐骨神经单纤维病变率4.9%(98/2000),明显低于亲代株组(16%,320/2000),差异有统计学意义,后者主要为轴索变性;④亲代株组半薄切片显示以轴索变性为特征的病理改变;变异株组仅见极少数异常,两组间差异有统计学意义;⑤电镜检查证实光镜所见。结论与亲代株相比,galE变异株缺失神经节苷脂GM1样表位,不能诱导动物产生GM1抗体及周围神经损伤,支持CJ感染后格林巴利综合征发生的分子模拟理论。