传染性病毒病是制约鸡养殖业发展的重要因素之一,严重影响鸡群健康和养殖效益。基因编辑技术在家禽抗病育种中的应用是现代禽类育种和疫情防控研究的热点。CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRIS...传染性病毒病是制约鸡养殖业发展的重要因素之一,严重影响鸡群健康和养殖效益。基因编辑技术在家禽抗病育种中的应用是现代禽类育种和疫情防控研究的热点。CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)作为一种高效精准的基因编辑工具,在诸多禽病毒病研究和防控中发挥重要作用。笔者简要综述了CRISPR/Cas9技术靶向敲除鸡病毒受体基因、修饰抗病毒相关基因、改造病毒基因组等方面的应用研究,展望该技术在抗病新品系培育和禽类重组病毒载体疫苗研发等方面的应用前景,为禽类病毒病的基础研究、预防和治疗提供参考依据。展开更多
CRISPR (clustered regularly interspaced short palindromic repeats)‐Cas (CRISPR associated protein) systems serve as the adaptive immune system by which prokaryotes defend themselves against phages. It has also been d...CRISPR (clustered regularly interspaced short palindromic repeats)‐Cas (CRISPR associated protein) systems serve as the adaptive immune system by which prokaryotes defend themselves against phages. It has also been developed into a series of powerful gene‐editing tools. As the natural inhibitors of CRISPR‐Cas systems, anti‐CRISPRs (Acrs) can be used as the “off‐switch” for CRISPR‐Cas systems to limit the off‐target effects caused by Cas9. Since the discovery of CRISPR‐Cas systems, much research has focused on the identification, mechanisms and applications of Acrs. In light of the rapid development and scientific significance of this field, this review summarizes the history and research status of Acrs, and considers future applications.展开更多
A one-pot synthesis method was conceptualized and implemented to develop green carbon-based nanocomposites working as biosensors.Porphyrin was synthesized to adorn the surface of nanocomposites making them highly sens...A one-pot synthesis method was conceptualized and implemented to develop green carbon-based nanocomposites working as biosensors.Porphyrin was synthesized to adorn the surface of nanocomposites making them highly sensitive for giving rise to π-π interactions between the genetic materials,proteins and porphyrin rings.The hydrogen bond formed between the proteins(analytes)and the nitrogen in the porphyrin structure as well as the surface hydroxyl groups was equally probable.In this context,different forms of porphyrins were incorporated to explore the interrelationship between the surface morphology and the ability of detection of genetic material and/or proteins by the aid of the synthesized structures.This phenomenon was conceptualized to optimize the interactions between the biomolecules and the substrate by reaching significant biosensor application in the presence of Anti-cas9 protein and sgRNA(concentration changed between 10 and 500 n mol/L).Almost full quenching of fluorescence emission was observed after addition of 300 n mol/L of Anti-cas9 protein and 250 n mol/L of sgRNA.Surprisingly,CoNi_(2)S_(4)provided 12%-29%cytotoxicity in both HEK-293 and PC12 cell lines.展开更多
文摘传染性病毒病是制约鸡养殖业发展的重要因素之一,严重影响鸡群健康和养殖效益。基因编辑技术在家禽抗病育种中的应用是现代禽类育种和疫情防控研究的热点。CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)作为一种高效精准的基因编辑工具,在诸多禽病毒病研究和防控中发挥重要作用。笔者简要综述了CRISPR/Cas9技术靶向敲除鸡病毒受体基因、修饰抗病毒相关基因、改造病毒基因组等方面的应用研究,展望该技术在抗病新品系培育和禽类重组病毒载体疫苗研发等方面的应用前景,为禽类病毒病的基础研究、预防和治疗提供参考依据。
基金Strategic Priority Research Programs of the Chinese Academy of Sciences,Grant/Award Number:XDA19050301National Natural Science Foundation of China,Grant/Award Number:31601189,81572433 and 81772646+1 种基金Biological Resources Program from Chinese Academy of Sciencesthe Young Elite Scientist Sponsorship Program by CAST,Grant/Award Number:2018QNRC001
文摘CRISPR (clustered regularly interspaced short palindromic repeats)‐Cas (CRISPR associated protein) systems serve as the adaptive immune system by which prokaryotes defend themselves against phages. It has also been developed into a series of powerful gene‐editing tools. As the natural inhibitors of CRISPR‐Cas systems, anti‐CRISPRs (Acrs) can be used as the “off‐switch” for CRISPR‐Cas systems to limit the off‐target effects caused by Cas9. Since the discovery of CRISPR‐Cas systems, much research has focused on the identification, mechanisms and applications of Acrs. In light of the rapid development and scientific significance of this field, this review summarizes the history and research status of Acrs, and considers future applications.
文摘A one-pot synthesis method was conceptualized and implemented to develop green carbon-based nanocomposites working as biosensors.Porphyrin was synthesized to adorn the surface of nanocomposites making them highly sensitive for giving rise to π-π interactions between the genetic materials,proteins and porphyrin rings.The hydrogen bond formed between the proteins(analytes)and the nitrogen in the porphyrin structure as well as the surface hydroxyl groups was equally probable.In this context,different forms of porphyrins were incorporated to explore the interrelationship between the surface morphology and the ability of detection of genetic material and/or proteins by the aid of the synthesized structures.This phenomenon was conceptualized to optimize the interactions between the biomolecules and the substrate by reaching significant biosensor application in the presence of Anti-cas9 protein and sgRNA(concentration changed between 10 and 500 n mol/L).Almost full quenching of fluorescence emission was observed after addition of 300 n mol/L of Anti-cas9 protein and 250 n mol/L of sgRNA.Surprisingly,CoNi_(2)S_(4)provided 12%-29%cytotoxicity in both HEK-293 and PC12 cell lines.
