Antiviral Oral Liquid(AOL)is an adult medicine in the Chinese Pharmacopoeia used to treat upper respiratory infections such as influenza.It has shown promising clinical efficacy in relieving flu-like symptoms such as ...Antiviral Oral Liquid(AOL)is an adult medicine in the Chinese Pharmacopoeia used to treat upper respiratory infections such as influenza.It has shown promising clinical efficacy in relieving flu-like symptoms such as fever,inflammation,and pharyngalgia both in adults and children.However,the instruction manual does not specify the exact usage and dosage of AOL for children.In this article,we set 6 dosage ranges:0.2,0.5,0.7,0.9,1.1,1.4 mL/kg/d,according to its dosage for adults and the conversion method between adult and children dosage.And six animal models were used to evaluate the effectiveness of AOL in different doses.The results indicated that AOL could reduce the lung index,virus load,and expression of proinflammatory cytokines in the lung.AOL could improve pathological changes and prolong the survival time of mice infected by the Influenza A virus(H1N1)A/PR/8/34 strains at 0.5–0.9 mL/kg/d concentrations in different degrees.The four dose groups of 0.7–1.4 mL/kg/d could significantly inhibit the ear shell swelling caused by xylene and reduce the rabbit body temperature induced by lipopolysaccharide(P<0.01,P<0.05).All the five dosage groups of 0.2–1.1 mL/kg/d could inhibit the increase of peritoneal capillary permeability induced by glacial acetic acid(P<0.01).AOL at 0.7 and 0.9 mL/kg/d reduced the painful writhing times in young mice induced by glacial acetic(P<0.01).These results indicated that the optimal dose of AOL in antiviral,antipyretic,anti-inflammatory,and analgesic effects is 0.7 mL/kg/d.展开更多
Backgroud:To explore the analgesic,anti-inflammatory,and anti-infective effects of Chai Ge fever relief oral liquid and provide evidence for clinical application of Chai Ge fever relief oral liquid.Methods:In this stu...Backgroud:To explore the analgesic,anti-inflammatory,and anti-infective effects of Chai Ge fever relief oral liquid and provide evidence for clinical application of Chai Ge fever relief oral liquid.Methods:In this study,four groups of experiments were designed:analgesic,anti-inflammatory,antiviral,and antibacterial.In the mouse pain model,the analgesic effect of Chai Ge fever relief oral liquid was studied by the writhing method and pain threshold;the anti-inflammatory effect by measuring the level of capillary permeability in the abdominal cavity of mice in different dose groups and the weight of cotton ball granuloma formation in rats;the antiviral effect by measuring the lung index of a pneumonia model of mouse infected with influenza virus;and the antibacterial effect by comparing the difference in the death protection ratio between each dose group and the model group result.Results:In the analgesic experiment,the high and medium dose of Chai Ge fever relief oral liquid could significantly reduce the number of writhing in mice caused by acetic acid,and the pain threshold of mice in the high-and medium-dose groups was significantly increased for 1–3 hours.In the anti-inflammatory experiment,the medium-dose group could significantly inhibit the increase of capillary permeability in the abdominal cavity of mice caused by acetic acid,and the low-dose group could significantly reduce the weight of rat cotton ball granuloma.In the antiviral experiment,the high-and medium-doses of Chai Ge fever relief oral liquid could significantly reduce the lung index of normal mouse pneumonia model of influenza virus infection and achieve a higher inhibition rate.In the anti-infective experiment,the death protection rate of the high-dose group was significantly different from that of the model control group.All three dose groups could significantly prolong the survival days of infected mice.Conclusion:These experimental results prove that in addition to its antipyretic effect,Chai Ge fever relief oral liquid also has analgesic,anti-inflammatory,antiviral,and antibacterial effects.展开更多
基金support of ABSL-2 biosafety laboratory of the Institute of Chinese Materia Medica.National Natural Science Foundation of China(No.82104500)Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(No.CI2021B015).
文摘Antiviral Oral Liquid(AOL)is an adult medicine in the Chinese Pharmacopoeia used to treat upper respiratory infections such as influenza.It has shown promising clinical efficacy in relieving flu-like symptoms such as fever,inflammation,and pharyngalgia both in adults and children.However,the instruction manual does not specify the exact usage and dosage of AOL for children.In this article,we set 6 dosage ranges:0.2,0.5,0.7,0.9,1.1,1.4 mL/kg/d,according to its dosage for adults and the conversion method between adult and children dosage.And six animal models were used to evaluate the effectiveness of AOL in different doses.The results indicated that AOL could reduce the lung index,virus load,and expression of proinflammatory cytokines in the lung.AOL could improve pathological changes and prolong the survival time of mice infected by the Influenza A virus(H1N1)A/PR/8/34 strains at 0.5–0.9 mL/kg/d concentrations in different degrees.The four dose groups of 0.7–1.4 mL/kg/d could significantly inhibit the ear shell swelling caused by xylene and reduce the rabbit body temperature induced by lipopolysaccharide(P<0.01,P<0.05).All the five dosage groups of 0.2–1.1 mL/kg/d could inhibit the increase of peritoneal capillary permeability induced by glacial acetic acid(P<0.01).AOL at 0.7 and 0.9 mL/kg/d reduced the painful writhing times in young mice induced by glacial acetic(P<0.01).These results indicated that the optimal dose of AOL in antiviral,antipyretic,anti-inflammatory,and analgesic effects is 0.7 mL/kg/d.
基金This study was supported by National Natural Science Foundation of China(81773977).
文摘Backgroud:To explore the analgesic,anti-inflammatory,and anti-infective effects of Chai Ge fever relief oral liquid and provide evidence for clinical application of Chai Ge fever relief oral liquid.Methods:In this study,four groups of experiments were designed:analgesic,anti-inflammatory,antiviral,and antibacterial.In the mouse pain model,the analgesic effect of Chai Ge fever relief oral liquid was studied by the writhing method and pain threshold;the anti-inflammatory effect by measuring the level of capillary permeability in the abdominal cavity of mice in different dose groups and the weight of cotton ball granuloma formation in rats;the antiviral effect by measuring the lung index of a pneumonia model of mouse infected with influenza virus;and the antibacterial effect by comparing the difference in the death protection ratio between each dose group and the model group result.Results:In the analgesic experiment,the high and medium dose of Chai Ge fever relief oral liquid could significantly reduce the number of writhing in mice caused by acetic acid,and the pain threshold of mice in the high-and medium-dose groups was significantly increased for 1–3 hours.In the anti-inflammatory experiment,the medium-dose group could significantly inhibit the increase of capillary permeability in the abdominal cavity of mice caused by acetic acid,and the low-dose group could significantly reduce the weight of rat cotton ball granuloma.In the antiviral experiment,the high-and medium-doses of Chai Ge fever relief oral liquid could significantly reduce the lung index of normal mouse pneumonia model of influenza virus infection and achieve a higher inhibition rate.In the anti-infective experiment,the death protection rate of the high-dose group was significantly different from that of the model control group.All three dose groups could significantly prolong the survival days of infected mice.Conclusion:These experimental results prove that in addition to its antipyretic effect,Chai Ge fever relief oral liquid also has analgesic,anti-inflammatory,antiviral,and antibacterial effects.
