Biomimetic Synthesis of Silver Nanoparticle Using Leaf Extract of Mangifera Indica and Their Antibacterial Efficacy

The aim of this study to focused on bioinspired synthesis of silver nanoparticles(AgNPs)as a viable alternative to eradicate the existing physicochemical processes.In this context,the bioinspired AgNPs were synthesized using leaf extract of M.indica.Optimization of the experimental conditions for the rapid and high yield of AgNPs in minimum investment of time and expense have been carried out along with their antibacterial efficacy evaluated.For this reason,the variation of parameters like the concentration of the silver precursors,reducing agent,time,pH,and temperature of synthesis were realized.Synthesized AgNPs were characterized by UV-Visible spectroscopy,X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FTIR)and transmission electron microscopy(TEM)techniques.UV-Visible spectra gave surface plasmon resonance(SPR)at 440 nm for AgNPs.Fourier transform infrared spectroscopy(FTIR)and X-ray diffraction(XRD)techniques were further confirmed the synthesis and crystalline nature of AgNPs respectively.Transmission electron microscopy(TEM)observed spherical shapes of synthesized AgNPs within range 5～20 nm.The results of the current study indicate that optimization process play apivotal role in the AgNPs synthesis and biogenic synthesized AgNPs might be used against bacterial pathogens.

ε-Poly(L-lysine)-based Hydrogels with Fast-acting and Prolonged Antibacterial Activities

Bacterial infections and the associated morbidity and mortality due to bacterial pathogens in wounds and medical implants have been increasing as most of current coatings cannot fulfill all the requirements including excellent intrinsically antibacterial activity, low cytotoxicity, and favorable physical properties. Herein, we present a kind of antibacterial bydrogel based on e-poly（L-lysine） （EPL） grafted carboxymethyl chitosan （CMC-g-EPL） as the inherently antibacterial matrix and the surplus EPL as highly efficient antimicrobial agent. Such hydrogels possess tunable swelling abilities with water absorption percentages of 800%-2000% and modulus varying from 10 kPa to 100 kPa, and exhibit two-stage excellent antibacterial behavior. First, the free EPL can be released from the hydrogel network for quick and highly efficient bacteria killing with 99.99% of efficacy; second, the grafted EPL endows bydrogel matrix with prolonged intrinsically antibacterial activity, especially when most of free EPL is released from the hydrogel. Overall, we provide a new insight for preparing highly effective antibacterial hydrogels.