Aim A liquid chromatographic method for the determination ofcandicidin/FR-008 and related components in fermentation broth has been developed. Methods Therewere four major components in the candicidin/FR-008 complex, ...Aim A liquid chromatographic method for the determination ofcandicidin/FR-008 and related components in fermentation broth has been developed. Methods Therewere four major components in the candicidin/FR-008 complex, which were separated by HPLC under thefollowing conditions: SB-C8 column (4.6 mm x 250 mm, 5 μm) was used, the mobile phase consisted ofacetonitrileam-monium acteate (20 mmol·L^(-1) , pH 4.0) (40:60, V/V) , with a flow rate of 1 .0mL·min^(-1) , the UV detection wavelength was 380 nm, and the whole process was performed at 25℃ .Results The linearity was obtained in the range of 6.25 - 500 μg· mL^(-1) candicidin/FR-008 withthe regression equation of Y = 20 461 x + 30 748 and the correlation coefficient of 0.999 1. Theinstrument precision was 1.84% and the method precision was 3.8%. Conclusion This method isaccurate, rapid and simple; it can be used for determination of candicidin/FR-008 and relatedcomponents in fermentation broth.展开更多
本文报道吸水链霉菌应城变种(Streptomyces hygroscopicus var yingchengensis)种内融合子FR-008产生的新抗生素(简称FR-008抗生素)对3种蚊虫幼虫的灭蚊活性以及蚊龄、温度、紫外线对FR-008抗生素灭蚊活性的影响。实验表明:FR-008抗生素...本文报道吸水链霉菌应城变种(Streptomyces hygroscopicus var yingchengensis)种内融合子FR-008产生的新抗生素(简称FR-008抗生素)对3种蚊虫幼虫的灭蚊活性以及蚊龄、温度、紫外线对FR-008抗生素灭蚊活性的影响。实验表明:FR-008抗生素对3种蚊虫幼虫均有较强的致死作用。对3龄致倦库蚊幼虫的LD_(50),24h为1.3309μg/ml,48hLD_(50)为0.5021μg/ml;对3龄白纹伊蚊幼虫的LD_(50),24h为2.0113μg/ml,48hLD_(50)为0.6321μg/ml;对3龄中华按蚊幼虫LD_(50),24h为2.8317μg/ml。不同虫龄对PR-008抗生素的敏感性不同,1龄至2龄幼虫比4龄初幼虫敏感。48h,1龄至2龄致倦库蚊幼虫LD_(50)为0.9055μg/ml;4龄初致倦库蚊幼虫LD_(50)为4.7124μg/ml。FR-008抗生素,在19～31℃之间,随温度升高灭蚊效果更好。经紫外线照射4h的2.5μg/ml FR-008抗生素稀释液,其活性降低为非照射组的51.4%。展开更多
文摘Aim A liquid chromatographic method for the determination ofcandicidin/FR-008 and related components in fermentation broth has been developed. Methods Therewere four major components in the candicidin/FR-008 complex, which were separated by HPLC under thefollowing conditions: SB-C8 column (4.6 mm x 250 mm, 5 μm) was used, the mobile phase consisted ofacetonitrileam-monium acteate (20 mmol·L^(-1) , pH 4.0) (40:60, V/V) , with a flow rate of 1 .0mL·min^(-1) , the UV detection wavelength was 380 nm, and the whole process was performed at 25℃ .Results The linearity was obtained in the range of 6.25 - 500 μg· mL^(-1) candicidin/FR-008 withthe regression equation of Y = 20 461 x + 30 748 and the correlation coefficient of 0.999 1. Theinstrument precision was 1.84% and the method precision was 3.8%. Conclusion This method isaccurate, rapid and simple; it can be used for determination of candicidin/FR-008 and relatedcomponents in fermentation broth.
文摘本文报道吸水链霉菌应城变种(Streptomyces hygroscopicus var yingchengensis)种内融合子FR-008产生的新抗生素(简称FR-008抗生素)对3种蚊虫幼虫的灭蚊活性以及蚊龄、温度、紫外线对FR-008抗生素灭蚊活性的影响。实验表明:FR-008抗生素对3种蚊虫幼虫均有较强的致死作用。对3龄致倦库蚊幼虫的LD_(50),24h为1.3309μg/ml,48hLD_(50)为0.5021μg/ml;对3龄白纹伊蚊幼虫的LD_(50),24h为2.0113μg/ml,48hLD_(50)为0.6321μg/ml;对3龄中华按蚊幼虫LD_(50),24h为2.8317μg/ml。不同虫龄对PR-008抗生素的敏感性不同,1龄至2龄幼虫比4龄初幼虫敏感。48h,1龄至2龄致倦库蚊幼虫LD_(50)为0.9055μg/ml;4龄初致倦库蚊幼虫LD_(50)为4.7124μg/ml。FR-008抗生素,在19～31℃之间,随温度升高灭蚊效果更好。经紫外线照射4h的2.5μg/ml FR-008抗生素稀释液,其活性降低为非照射组的51.4%。
