We propose a new design of the high channel capacity in Mobile Ad Hoc Network that uses the dense wave-length division multiplexing wavelength enhancement, in which the increasing in channel capacity and sig-nal secur...We propose a new design of the high channel capacity in Mobile Ad Hoc Network that uses the dense wave-length division multiplexing wavelength enhancement, in which the increasing in channel capacity and sig-nal security can be provided. The increasing in number of channel can be obtained by the increasing in wavelength density, while the security is introduced by the specific wavelength filter, which is operated by the Ad Hoc node operator and link with other nodes in coverage by dedicated one-to-one in direct or relay node. The optical communication wavelength enhancement is reviewed. The advantage is that the proposed system can be implemented and used incorporating with the existed communication link in both infrastruc-ture-based and Ad Hoc networks wireless network, where the privacy can be provided, which is discussed in details.展开更多
Mono-, di-, tri-ethanolamines and di-, tri-ethylamines were used to prepare phosphate buffer at pH 2.5 and shown to be capable of enhancing the separation selectivity of chiral drug pinacidil and melittin in bee venom...Mono-, di-, tri-ethanolamines and di-, tri-ethylamines were used to prepare phosphate buffer at pH 2.5 and shown to be capable of enhancing the separation selectivity of chiral drug pinacidil and melittin in bee venom in capillary zone electrophoresis. The reason is due to the reduction of the average apparent mobilities of the analytes.展开更多
The technology of radio frequency （RF） radiation intensification for radio compact antennas based on modulation and enhancement effects of sub-wavelength plasma structures represents an innovative developing strateg...The technology of radio frequency （RF） radiation intensification for radio compact antennas based on modulation and enhancement effects of sub-wavelength plasma structures represents an innovative developing strategy. It exhibits important scientific significance and promising potential of broad applications in various areas of national strategic demands, such as electrical information network and microwave communication, detection and control technology. In this paper, laboratory experiments and corresponding analyses have been carried out to investigate the modulation and enhancement technology of sub-wavelength plasma structure on the RF electromagnetic radiation. An application focused sub-wavelength plasma-added intensification up to ~7 dB higher than the free-space radiation is observed experimentally in giga-hertz （GHz） RF band. The effective radiation enhancement bandwidth covers from 0.85 to 1.17 GHz, while the enhanced electromagnetic signals transmitted by sub-wavelength plasma structures maintain good communication quality. Particularly, differing from the traditional RF electromagnetic radiation enhancement method characterized by focusing the radiation field of antenna in a specific direction, the sub-wavelength plasma-added intensification of the antenna radiation presents an omnidirectional enhancement, which is reported experimentally for the first time. Corresponding performance characteristics and enhancement mechanism analyses are also conducted in this paper. The results have demonstrated the feasibility and promising potential of sub-wavelength plasma modulation in application focused RF communication, and provided the scientific basis for further research and development of sub-wavelength plasma enhanced compact antennas with wide-range requests and good quality for communication.展开更多
Porcine reproductive and respiratory syndrome(PRRS)is one of the most significant diseases affecting the pig industry worldwide.The PRRSV mutation rate is the highest among the RNA viruses.To date,NADC30-like PRRSV an...Porcine reproductive and respiratory syndrome(PRRS)is one of the most significant diseases affecting the pig industry worldwide.The PRRSV mutation rate is the highest among the RNA viruses.To date,NADC30-like PRRSV and highly pathogenic PRRSV(HP-PRRSV)are the dominant epidemic strains in China;however,commercial vaccines do not always provide sufficient cross-protection,and the reasons for insufficient protection are unclear.This study isolated a wild-type NADC30-like PRRSV,SX-YL1806,from Shaanxi Province.Vaccination challenge experiments in piglets showed that commercial modified live virus(MLV)vaccines provided good protection against HP-PRRSV.However,it could not provide sufficient protection against the novel strain SXYL1806.To explore the reasons for this phenomenon,we compared the genomic homology between the MLV strain and HP-PRRSV or NADC30-like PRRSV and found that the MLV strain had a lower genome similarity with NADC30-like PRRSV.Serum neutralization assay showed that MLV-immune serum slightly promoted the homologous HP-PRRSV replication and significantly promoted the heterologous NADC30-like PRRSV strain replication in vitro,suggesting that antibody-dependent enhancement(ADE)might also play a role in decreasing MLV protective efficacy.These findings expand our understanding of the potential factors affecting the protective effect of PRRSV MLV vaccines against the NADC30-like strains.展开更多
The amyloid hypothesis of Alzheimer＇s disease （AD） pathogen- esis maintains that the key event is the production of specific C-terminal amyloid-β （Aβ） peptides following the abnormal proteolytic cleavage of the...The amyloid hypothesis of Alzheimer＇s disease （AD） pathogen- esis maintains that the key event is the production of specific C-terminal amyloid-β （Aβ） peptides following the abnormal proteolytic cleavage of the amyloid precursor protein （Vassar and Citron, 2000）.展开更多
A new algorithm called spatially aware routing algorithm with enhanced learning (SAREL) is proposed to guarantee the rationality of route selecting in inter-vehicle communication scenario. Firstly, the traffic model i...A new algorithm called spatially aware routing algorithm with enhanced learning (SAREL) is proposed to guarantee the rationality of route selecting in inter-vehicle communication scenario. Firstly, the traffic model is discussed and set up by using Poisson distribution. Then we analyze the process of traffic evaluation with enhanced learning, and exploit movement estimation to assist state memorization. The improvement of algorithm is provided at last compared with our previous work. Simulation results show that SAREL algorithm could achieve better performance in packet delivery ratio, especially when network connection ratio is average. Key words mobile ad hoc network - spatially aware routing - enhanced learning CLC number TP 316 Foundation item: Supported by Open Laboratory Foundation by China Ministry of Education (TKLJ9903), Project CarTALK 2000 by the European Commission (IST-2000-28185) and Project FleetNet-Internet on the Road by the German Ministry of Education and Research (01AK025)Biography: HAN Lu (1974-), male, Ph. D candidate, research direction; distributed artificial intelligence.展开更多
Objective: To evaluate the expression of DNA plasmid-harboring modified antibody gene that produces neutralizing human monoclonal antibodies against four serotypes of dengue virus(DENV) without enhancing activity in B...Objective: To evaluate the expression of DNA plasmid-harboring modified antibody gene that produces neutralizing human monoclonal antibodies against four serotypes of dengue virus(DENV) without enhancing activity in BALB/c mice. Methods: We constructed pFUSE-based vectors(pFUSE1 G7 C2hVH and pFUSE1 G7 C2hVL) containing genes encoding the variable domains of the heavy or light chain of the anti-dengue virus antibody 1 G7 C2, a human IgG1 that has been characterized for its neutralizing activity to DENV-1-4. Leucine(L) at positions 234 and 235 on the Fc CH2 domain in pFUSE1 G7 C2hVH was mutated to alanine(A)(LALA mutation) by site direct mutagenesis, and the new plasmid was termed pFUSE1 G7 C2hVHLALA. An equal amount of pFUSE1 G7 C2hVL and 1 G7 C2hG1-LALA plasmids were co-transfected into Chinese hamster ovary cells(CHO-K1) and a single dose of 100 μg 1 G7 C2hG1-LALA plasmid was intramuscularly injected, followed by electroporation in BALB/c mice. The secreted 1 G7 C2hG1-LALA antibodies in cell culture supernatant and mouse serum were examined for their biological functions, neutralization and enhancing activity. Results: The co-transfection of heavy-and light-chain 1 G7 C2hG1-LALA plasmids in CHO-K1 cells produced approximately 3 900 ng/mL human IgG and neutralized 90%-100% all four DENV, with no enhancing activity. Furthermore, the modified human IgG was produced more than 1 000 ng/mL in mouse serum on day 7 post plasmid injection and showed cross-neutralization to four DENV serotypes. Subsequently, antibody production and neutralization decreased rapidly. Nevertheless, the secreted neutralizing 1 G7 C2hG1-LALA in mouse serum demonstrated complete absence of enhancing activities to all DENV serotypes. Conclusions: These findings reveal that a new modified 1 G7 C2h G1-LALA expressing plasmid based on gene transfer is a possible therapeutic antibody candidate against DENV infection.展开更多
IEEE 802.11 distributed coordination function(DCF)can alleviate the collision and hidden station problem,but it doesn't differentiate traffic categories(TC).Therefore,it can't provide sufficient quality of ser...IEEE 802.11 distributed coordination function(DCF)can alleviate the collision and hidden station problem,but it doesn't differentiate traffic categories(TC).Therefore,it can't provide sufficient quality of service(QoS)support for different TC.Recently,a new contention-based enhanced distributed channel access(EDCA)scheme is proposed which provides a probabilistic QoS support.In this paper,an improved EDCA scheme for service differentiation in ad hoc networks is proposed.In this scheme,signal channel resistance coefficient is used to adjust the contention window(CW).It proves that the scheme provides the traffic differentiation,high throughput and low delay through simulation.展开更多
In order to solve the bad low frequency sound absorption of the Micro-Perforated panel （MPP） absorber, mechanical impedance was introduced in the back of the MPP absorber to form a composite structure. According to ...In order to solve the bad low frequency sound absorption of the Micro-Perforated panel （MPP） absorber, mechanical impedance was introduced in the back of the MPP absorber to form a composite structure. According to the same particle vibration velocity on both sides of a plate, the mechanical impedance plate transfer matrix could be obtained. The units of the mechanical impedance, cavity and MPP were connected in series with the use of the transfer matrix method, thus creating the composite structure＇s theoretical calculation model. The qual- ity factor affecting absorption bandwidth was analyzed. Bandwidth is inversely proportional to the mechanical impedance plate mass. During the experiments, when at close to 400 Hz, the composite structure reached an absorption peak with a coefficient of above 0.8. Experimen- tal results concurred with theoretical calculations. Mechanical resonance is added based on the traditional MPP resonance sound absorption mechanism. Through this, the performance of low frequency sound absorption can be improved without increasing the thickness of the structure. The frequency band can be broadened by reducing the mechanical impedance plate mass and controlling its boundary-damping coefficient.展开更多
文摘We propose a new design of the high channel capacity in Mobile Ad Hoc Network that uses the dense wave-length division multiplexing wavelength enhancement, in which the increasing in channel capacity and sig-nal security can be provided. The increasing in number of channel can be obtained by the increasing in wavelength density, while the security is introduced by the specific wavelength filter, which is operated by the Ad Hoc node operator and link with other nodes in coverage by dedicated one-to-one in direct or relay node. The optical communication wavelength enhancement is reviewed. The advantage is that the proposed system can be implemented and used incorporating with the existed communication link in both infrastruc-ture-based and Ad Hoc networks wireless network, where the privacy can be provided, which is discussed in details.
文摘Mono-, di-, tri-ethanolamines and di-, tri-ethylamines were used to prepare phosphate buffer at pH 2.5 and shown to be capable of enhancing the separation selectivity of chiral drug pinacidil and melittin in bee venom in capillary zone electrophoresis. The reason is due to the reduction of the average apparent mobilities of the analytes.
基金supported by National Natural Science Foundation of China under Grant No. 51577044
文摘The technology of radio frequency （RF） radiation intensification for radio compact antennas based on modulation and enhancement effects of sub-wavelength plasma structures represents an innovative developing strategy. It exhibits important scientific significance and promising potential of broad applications in various areas of national strategic demands, such as electrical information network and microwave communication, detection and control technology. In this paper, laboratory experiments and corresponding analyses have been carried out to investigate the modulation and enhancement technology of sub-wavelength plasma structure on the RF electromagnetic radiation. An application focused sub-wavelength plasma-added intensification up to ~7 dB higher than the free-space radiation is observed experimentally in giga-hertz （GHz） RF band. The effective radiation enhancement bandwidth covers from 0.85 to 1.17 GHz, while the enhanced electromagnetic signals transmitted by sub-wavelength plasma structures maintain good communication quality. Particularly, differing from the traditional RF electromagnetic radiation enhancement method characterized by focusing the radiation field of antenna in a specific direction, the sub-wavelength plasma-added intensification of the antenna radiation presents an omnidirectional enhancement, which is reported experimentally for the first time. Corresponding performance characteristics and enhancement mechanism analyses are also conducted in this paper. The results have demonstrated the feasibility and promising potential of sub-wavelength plasma modulation in application focused RF communication, and provided the scientific basis for further research and development of sub-wavelength plasma enhanced compact antennas with wide-range requests and good quality for communication.
基金This research was supported by the National Natural Science Foundation of China(32172846)the Earmarked Fund for CARS-35,the Science and Technology Major Project of Gansu Province(22ZD6NA001)+4 种基金the Science Foundation for Distinguished Young Scholars of Shaanxi Province(2021JC-18)the Natural Science Foundation of Gansu Province(23JRRA1153)the Science and Technology Plan Project of Gansu Province(23JRRA561)the Chinese Academy of Agricultural Science and Technology Innovation Project(CAAS-ASTIP-JBGS-20210602)the Strategic Priority Research Program of the National Center of Technology Innovation for Pigs(NCTIP-XD/C03).
文摘Porcine reproductive and respiratory syndrome(PRRS)is one of the most significant diseases affecting the pig industry worldwide.The PRRSV mutation rate is the highest among the RNA viruses.To date,NADC30-like PRRSV and highly pathogenic PRRSV(HP-PRRSV)are the dominant epidemic strains in China;however,commercial vaccines do not always provide sufficient cross-protection,and the reasons for insufficient protection are unclear.This study isolated a wild-type NADC30-like PRRSV,SX-YL1806,from Shaanxi Province.Vaccination challenge experiments in piglets showed that commercial modified live virus(MLV)vaccines provided good protection against HP-PRRSV.However,it could not provide sufficient protection against the novel strain SXYL1806.To explore the reasons for this phenomenon,we compared the genomic homology between the MLV strain and HP-PRRSV or NADC30-like PRRSV and found that the MLV strain had a lower genome similarity with NADC30-like PRRSV.Serum neutralization assay showed that MLV-immune serum slightly promoted the homologous HP-PRRSV replication and significantly promoted the heterologous NADC30-like PRRSV strain replication in vitro,suggesting that antibody-dependent enhancement(ADE)might also play a role in decreasing MLV protective efficacy.These findings expand our understanding of the potential factors affecting the protective effect of PRRSV MLV vaccines against the NADC30-like strains.
文摘The amyloid hypothesis of Alzheimer＇s disease （AD） pathogen- esis maintains that the key event is the production of specific C-terminal amyloid-β （Aβ） peptides following the abnormal proteolytic cleavage of the amyloid precursor protein （Vassar and Citron, 2000）.
文摘A new algorithm called spatially aware routing algorithm with enhanced learning (SAREL) is proposed to guarantee the rationality of route selecting in inter-vehicle communication scenario. Firstly, the traffic model is discussed and set up by using Poisson distribution. Then we analyze the process of traffic evaluation with enhanced learning, and exploit movement estimation to assist state memorization. The improvement of algorithm is provided at last compared with our previous work. Simulation results show that SAREL algorithm could achieve better performance in packet delivery ratio, especially when network connection ratio is average. Key words mobile ad hoc network - spatially aware routing - enhanced learning CLC number TP 316 Foundation item: Supported by Open Laboratory Foundation by China Ministry of Education (TKLJ9903), Project CarTALK 2000 by the European Commission (IST-2000-28185) and Project FleetNet-Internet on the Road by the German Ministry of Education and Research (01AK025)Biography: HAN Lu (1974-), male, Ph. D candidate, research direction; distributed artificial intelligence.
基金supported by the Faculty of Tropical Medicine,Mahidol University,Thailand,Research Fund through a Research and Researcher for Industry(RRi,Grant Number PHD59I0063 for SB)TRF Grant for New Researcher(TRG,Grant Number TRG5980015 for CP)the Office of the National Research Council of Thailand-Japan Society for the Promotion of Science(JSPS)or NRCT-JSPS
文摘Objective: To evaluate the expression of DNA plasmid-harboring modified antibody gene that produces neutralizing human monoclonal antibodies against four serotypes of dengue virus(DENV) without enhancing activity in BALB/c mice. Methods: We constructed pFUSE-based vectors(pFUSE1 G7 C2hVH and pFUSE1 G7 C2hVL) containing genes encoding the variable domains of the heavy or light chain of the anti-dengue virus antibody 1 G7 C2, a human IgG1 that has been characterized for its neutralizing activity to DENV-1-4. Leucine(L) at positions 234 and 235 on the Fc CH2 domain in pFUSE1 G7 C2hVH was mutated to alanine(A)(LALA mutation) by site direct mutagenesis, and the new plasmid was termed pFUSE1 G7 C2hVHLALA. An equal amount of pFUSE1 G7 C2hVL and 1 G7 C2hG1-LALA plasmids were co-transfected into Chinese hamster ovary cells(CHO-K1) and a single dose of 100 μg 1 G7 C2hG1-LALA plasmid was intramuscularly injected, followed by electroporation in BALB/c mice. The secreted 1 G7 C2hG1-LALA antibodies in cell culture supernatant and mouse serum were examined for their biological functions, neutralization and enhancing activity. Results: The co-transfection of heavy-and light-chain 1 G7 C2hG1-LALA plasmids in CHO-K1 cells produced approximately 3 900 ng/mL human IgG and neutralized 90%-100% all four DENV, with no enhancing activity. Furthermore, the modified human IgG was produced more than 1 000 ng/mL in mouse serum on day 7 post plasmid injection and showed cross-neutralization to four DENV serotypes. Subsequently, antibody production and neutralization decreased rapidly. Nevertheless, the secreted neutralizing 1 G7 C2hG1-LALA in mouse serum demonstrated complete absence of enhancing activities to all DENV serotypes. Conclusions: These findings reveal that a new modified 1 G7 C2h G1-LALA expressing plasmid based on gene transfer is a possible therapeutic antibody candidate against DENV infection.
文摘IEEE 802.11 distributed coordination function(DCF)can alleviate the collision and hidden station problem,but it doesn't differentiate traffic categories(TC).Therefore,it can't provide sufficient quality of service(QoS)support for different TC.Recently,a new contention-based enhanced distributed channel access(EDCA)scheme is proposed which provides a probabilistic QoS support.In this paper,an improved EDCA scheme for service differentiation in ad hoc networks is proposed.In this scheme,signal channel resistance coefficient is used to adjust the contention window(CW).It proves that the scheme provides the traffic differentiation,high throughput and low delay through simulation.
基金supported by the Scientific Research Foundation for Senior Professional of Jiangsu University(11JDG096)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions((2011)6)
文摘In order to solve the bad low frequency sound absorption of the Micro-Perforated panel （MPP） absorber, mechanical impedance was introduced in the back of the MPP absorber to form a composite structure. According to the same particle vibration velocity on both sides of a plate, the mechanical impedance plate transfer matrix could be obtained. The units of the mechanical impedance, cavity and MPP were connected in series with the use of the transfer matrix method, thus creating the composite structure＇s theoretical calculation model. The qual- ity factor affecting absorption bandwidth was analyzed. Bandwidth is inversely proportional to the mechanical impedance plate mass. During the experiments, when at close to 400 Hz, the composite structure reached an absorption peak with a coefficient of above 0.8. Experimen- tal results concurred with theoretical calculations. Mechanical resonance is added based on the traditional MPP resonance sound absorption mechanism. Through this, the performance of low frequency sound absorption can be improved without increasing the thickness of the structure. The frequency band can be broadened by reducing the mechanical impedance plate mass and controlling its boundary-damping coefficient.
