Delphinium brunonianum Royle belongs to Ranunculaceae family and has the effects of dispelling wind to relieve itching and cooling blood to detoxify.It was found that the extracts of D.brunonianum had good anticoagula...Delphinium brunonianum Royle belongs to Ranunculaceae family and has the effects of dispelling wind to relieve itching and cooling blood to detoxify.It was found that the extracts of D.brunonianum had good anticoagulant activity which was extracted with 70%ethanol in our previous researches.Then,16 compounds were isolated and identified from the extract of D.brunonianum,among which compounds 5,7-10,12,14,15-16 were isolated from this genus for the first time,and compounds 2-4 were isolated from this plant for the first time.And the coagulation activity assay showed that compounds 10,14 and 15 had good anticoagulant activity by activated partial thromboplastin time(APTT),thrombin time(TT)and prothrombin time(PT)in vitro.展开更多
Ten neolignans or norlignans(1-10)including eight new compounds were isolated from the whole bodies of Polyphaga plancyi Bolivar.Their structures were identified by spectroscopic data.Compounds 3,4,8,and 9 are racemat...Ten neolignans or norlignans(1-10)including eight new compounds were isolated from the whole bodies of Polyphaga plancyi Bolivar.Their structures were identified by spectroscopic data.Compounds 3,4,8,and 9 are racemates indicated by chiral HPLC analysis.Chiral separation followed by ECD calculations allowed to clarify the absolute configurations of all the antipodes.All the new compounds were evaluated for their biological properties toward extracellular matrix in rat renal proximal tubular cells,human cancer cells(K562,A549,and Huh7),EV71,ROCK2,JAK3,DDR1,and coagulation.展开更多
Objective:To study the spine structure of stingray Himantura imbricata(H.imbricata)and to evaluate the anticoagulant properties of the spine extract obtained through various solvents extracts followed by antibacterial...Objective:To study the spine structure of stingray Himantura imbricata(H.imbricata)and to evaluate the anticoagulant properties of the spine extract obtained through various solvents extracts followed by antibacterial activity against human pathogens.Methods:Spines of H.imbricata were collected from Nagappattinam coast,Tamil Nadu,India and their spines were observed under the light microscope.The grounded spines were subjected to extraction of metabolites using methanol,ethanol,chloroform and acetone.Antibacterial activity was evaluated by disc diffusion technique against 10 human pathogens.Similarly,anticoagulant activity was also assessed by following United States Pharmacopeia method.Results:Light microscopic observation of spine revealed that the venom apparatus of the stingray H.imbricata consisted of two to three spines,glandular tissue and a sheath.The spine extract showed potent antibacterial activity against all tested pathogen.Maximum activity(14 mm)was found against Staphylococcus aureus.Crude extract showed 91.50 USP units/mg of anticoagulant activity.Conclusions:Microscopic observations gave new insight about the spine structure of the stingray.The spine extracts of H.imbricate showed potent activity against human pathogens revealed by the good zone of inhibition.Chloroform extracts conferred the most prominent antibacterial activity.The anticoagulant activity was also comparable with that of standard heparin.展开更多
基金This work was funded by Research on Precision Nutrition and Health Food,Department of Science and Technology of Henan Province(CXJD2021006).
文摘Delphinium brunonianum Royle belongs to Ranunculaceae family and has the effects of dispelling wind to relieve itching and cooling blood to detoxify.It was found that the extracts of D.brunonianum had good anticoagulant activity which was extracted with 70%ethanol in our previous researches.Then,16 compounds were isolated and identified from the extract of D.brunonianum,among which compounds 5,7-10,12,14,15-16 were isolated from this genus for the first time,and compounds 2-4 were isolated from this plant for the first time.And the coagulation activity assay showed that compounds 10,14 and 15 had good anticoagulant activity by activated partial thromboplastin time(APTT),thrombin time(TT)and prothrombin time(PT)in vitro.
基金supported by the National Science Fund for Distinguished Young Scholars(81525026)Shenzhen Government’s Plan of Science and Technology(JCYJ20170412110504956).
文摘Ten neolignans or norlignans(1-10)including eight new compounds were isolated from the whole bodies of Polyphaga plancyi Bolivar.Their structures were identified by spectroscopic data.Compounds 3,4,8,and 9 are racemates indicated by chiral HPLC analysis.Chiral separation followed by ECD calculations allowed to clarify the absolute configurations of all the antipodes.All the new compounds were evaluated for their biological properties toward extracellular matrix in rat renal proximal tubular cells,human cancer cells(K562,A549,and Huh7),EV71,ROCK2,JAK3,DDR1,and coagulation.
基金Supported by Center with Potential for Excellence in Particular Area(CPEPA),Ministry of Environmental Forest(MOEF)(Grant No.G4(1)/1011/2012).
文摘Objective:To study the spine structure of stingray Himantura imbricata(H.imbricata)and to evaluate the anticoagulant properties of the spine extract obtained through various solvents extracts followed by antibacterial activity against human pathogens.Methods:Spines of H.imbricata were collected from Nagappattinam coast,Tamil Nadu,India and their spines were observed under the light microscope.The grounded spines were subjected to extraction of metabolites using methanol,ethanol,chloroform and acetone.Antibacterial activity was evaluated by disc diffusion technique against 10 human pathogens.Similarly,anticoagulant activity was also assessed by following United States Pharmacopeia method.Results:Light microscopic observation of spine revealed that the venom apparatus of the stingray H.imbricata consisted of two to three spines,glandular tissue and a sheath.The spine extract showed potent antibacterial activity against all tested pathogen.Maximum activity(14 mm)was found against Staphylococcus aureus.Crude extract showed 91.50 USP units/mg of anticoagulant activity.Conclusions:Microscopic observations gave new insight about the spine structure of the stingray.The spine extracts of H.imbricate showed potent activity against human pathogens revealed by the good zone of inhibition.Chloroform extracts conferred the most prominent antibacterial activity.The anticoagulant activity was also comparable with that of standard heparin.
