The title compound 2-（（2-fluorobenzyl）thio）-5-（pyridin-4-yl）-1,3,4-oxadiazole（C_（14）H_（10）FN_3OS） was synthesized,and its structure was confirmed by ~1H NMR,MS,elemental analyses and X-ray diffraction. It ...The title compound 2-（（2-fluorobenzyl）thio）-5-（pyridin-4-yl）-1,3,4-oxadiazole（C_（14）H_（10）FN_3OS） was synthesized,and its structure was confirmed by ~1H NMR,MS,elemental analyses and X-ray diffraction. It crystallizes in the monoclinic system,space group P21/n with a = 11.541（16）,b = 8.226（12）,c = 13.683（19） ？,β = 94.872（17）o,V = 1294（3） ？~3,Z = 4 and R = 0.0648 for 2198 observed reflections with I 〉 2σ（I）. The preliminary biological test shows that the title compound has good activity against Pythium ultimum with inhibitory to be 100%.展开更多
Penicillium expansum is the primary postharvest pathogen that attacks fruits and vegetables and threatens human health.Bifidobacterium adolescentis CCFM1108 has been previously demonstrated to inhibit the growth of Pe...Penicillium expansum is the primary postharvest pathogen that attacks fruits and vegetables and threatens human health.Bifidobacterium adolescentis CCFM1108 has been previously demonstrated to inhibit the growth of Penicillium expansum and patulin production;however,its modes of action remain unclear.RNA-seq analysis was used to investigate the underlying antifungal mechanism of metabolites produced by B .adolescents CCFM1108.The expression of genes in the control group and three treatment groups (1/2 MIC CFS,1/4 MIC CFS,and acid treatment groups) was determined and further compared using GO and KEGG analyses.The inhibitory metabolites were found to participate in the dysfunction of the mitochondrial membrane,affecting energy metabolism,reactive oxidative species production,and patulin biosynthesis.Compared with the mixture of multiple metabolites of Bifidobacterium ,the acid mixture affected gene expression in a more significant manner by inhibiting all critical genes included in the patulin biosynthetic pathway.The changes in the patulin biosynthetic were verified by qRT-PCR in the presence of acids.Collectively,the present study provides a clear perspective on the antifungal mechanism of metabolites produced by a B.adolescents strain through transcriptome analysis and suggests that acid mixtures could be potential candidates for the postharvest control of P .expansum .展开更多
基金funded by the Natural Science Foundation of Zhejiang Province(No.LY16C140007)the program of Science and Technology of Jiangsu Province(BY2014108-14)
文摘The title compound 2-（（2-fluorobenzyl）thio）-5-（pyridin-4-yl）-1,3,4-oxadiazole（C_（14）H_（10）FN_3OS） was synthesized,and its structure was confirmed by ~1H NMR,MS,elemental analyses and X-ray diffraction. It crystallizes in the monoclinic system,space group P21/n with a = 11.541（16）,b = 8.226（12）,c = 13.683（19） ？,β = 94.872（17）o,V = 1294（3） ？~3,Z = 4 and R = 0.0648 for 2198 observed reflections with I 〉 2σ（I）. The preliminary biological test shows that the title compound has good activity against Pythium ultimum with inhibitory to be 100%.
基金supported by National Natural Science Foundation of China Key Program(31772090,32001665,31820103010,U1903205)Natural Science Foundation of Jiangsu Province(BE2021623)+1 种基金the Key Scientific and Technological Research Projects(2018AB010)BBSRC Newton Fund Joint Centre Award.
文摘Penicillium expansum is the primary postharvest pathogen that attacks fruits and vegetables and threatens human health.Bifidobacterium adolescentis CCFM1108 has been previously demonstrated to inhibit the growth of Penicillium expansum and patulin production;however,its modes of action remain unclear.RNA-seq analysis was used to investigate the underlying antifungal mechanism of metabolites produced by B .adolescents CCFM1108.The expression of genes in the control group and three treatment groups (1/2 MIC CFS,1/4 MIC CFS,and acid treatment groups) was determined and further compared using GO and KEGG analyses.The inhibitory metabolites were found to participate in the dysfunction of the mitochondrial membrane,affecting energy metabolism,reactive oxidative species production,and patulin biosynthesis.Compared with the mixture of multiple metabolites of Bifidobacterium ,the acid mixture affected gene expression in a more significant manner by inhibiting all critical genes included in the patulin biosynthetic pathway.The changes in the patulin biosynthetic were verified by qRT-PCR in the presence of acids.Collectively,the present study provides a clear perspective on the antifungal mechanism of metabolites produced by a B.adolescents strain through transcriptome analysis and suggests that acid mixtures could be potential candidates for the postharvest control of P .expansum .
