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Separation of CD34 Positive Cells and Determination of Surface Homing Antigen
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作者 王良利 邹萍 游泳 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2000年第2期132-133,共2页
To study the differences in homing potential between bone marrow cells and umbilical blood cells, CD34 positive cells were obtained from bone marrow (BM) and umbilical blood (UB) by the direct cell separation with dom... To study the differences in homing potential between bone marrow cells and umbilical blood cells, CD34 positive cells were obtained from bone marrow (BM) and umbilical blood (UB) by the direct cell separation with domestic immunomagnetic beads. The expression of the two adhesion molecules CD11a/CD18 and CD44 were examined. After separation, CD34 positive cells accounted for 51 %-82 % of the harvested cells and dye-resistance rate was 82 %-88 %. The expression of CD11a/CD18 and CD44 on the surfaces of UB cells was 49. 6 % 1 10. 2 % and 37. 7 % ± 10. 3 % respectively. On BM cells they were 50. 2 % ± 6. 2 % and 34 % ± 13. 3 % respectively. There were no significant differences in the expression of these two molecules. It was concluded .that the cell separation method with domestic immunomagnetic beads was effective and the stem cells from UB could serve as an alternative source for transplantation. 展开更多
关键词 domestic immunomagnetic beads cell separation cd34 positive cells adhesion molecules
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Activation of killer cells with soluble gastric cancer antigen combined with anti-CD3 McAb 被引量:5
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作者 CHEN Qiang, YE Yun Bin and CHEN Zeng 《World Journal of Gastroenterology》 SCIE CAS CSCD 1999年第2期91-92,共2页
INTRODUCTIONTherehavebeenmanyreportsoncancertherapywithlymphokineactivatedkiler(LAK)celsandinterleukin2(IL... INTRODUCTIONTherehavebeenmanyreportsoncancertherapywithlymphokineactivatedkiler(LAK)celsandinterleukin2(IL2),buttheprolife... 展开更多
关键词 STOMACH neoplasms antigens NEOPLASM KILLER cells INTERLEUKIN 2 cd3 McAb
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Detection of microbial antigenic components of circulating immune complexes in HIV patients:Involvement in CD4^+ T lymphocyte count depletion
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作者 Ezeani Michael Chukwudi Onyenekwe CC +7 位作者 Wachukwu CK Anyiam DCD Meludu SC Ukibe RN Ifeanyichukwu M Onochie A Anahalu I Okafor UU 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2010年第10期828-832,共5页
Objective:To investigate the prevalence of microbial antigenic components of circulating immune complexes amongst grades of CD4 T lymphocyte counts in HIV sero positive and seronegative participants.Methods:Polyethele... Objective:To investigate the prevalence of microbial antigenic components of circulating immune complexes amongst grades of CD4 T lymphocyte counts in HIV sero positive and seronegative participants.Methods:Polyethelene glycol(PEG-600) and buffering methods of precipitation and dissociation of immune complexes was used to generate immune solution from sera of 100 HIV sero-positive and 100 HIV sero-negative participants.These were categorized into 3 grades based on CD4 count:】 500 cell/mm,200-499 cell/mm3 and 【200 cell/mm3.The immune solutions were assayed using membrane based immunoassay and antibody titration, along side its unprocessed serum for detection of various microbial antigens and or antibodies. CD4 T cell counts were estimated using Patec Cyflow SL-3 Germany.Results:Antigenic component of immune complexes of various infectious agents was detected in 99 and 70 HIV seropositive and HIV sero-negative participants,respectively.In group A,there were 10 HIV positive participants,including 4(40.0%) had circulating immune complexes(CICs) due to Salmonella species only:1(10.0%) due to Salmonella-Plasmodium falciparum(P.falciparum),SalmonellaP. falciparum-HCV and P.falciparum antigens,respectively.In group B,45(45.4%) HIV seropositive participants with CICs had CD4 T lymphocyte count between 200-499 cells/mm^3.Out of these,20(44.4%) had CICs due to Salmonella species only:9(20%) due to Salmonella-P. falciparum.In group C,there were 44(44.4%) HIV sero-positive participants,including 3(6.8%) due to Salmonella species only:24(54.4%) due to Salmonella-P.falciparum:2(4.5%) due to P. falciparum only.Conclusions:In HIV sero-positive participants,presence of heterogeneity of Salmonella species-P.falciparum antigens was highly incriminated in CD4 count depletion but not homogeneity of malaria parasites antigens.Malaria parasites antigens only were incriminated in CD4^+ count depletion amongst HIV sero-negative participants.Before taking any decision on the management of HIV-1-positive individuals,their malaria and Salmonella paratyphi status should be assessed,but not malaria status alone. 展开更多
关键词 HIV/AIDS Immune complexes MICROBIAL antigenS HIV positive PARTICIPANT cd4^+ LYMPHOCYTE COUNT
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Rejection of Experimental Hodgkins Lymphoma by T-Cells Engineered with a CD19 Chimeric Antigen Receptor
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作者 Anna Swanson Eleanor Cheadle +3 位作者 David Gilham Dorothy Crawford Simon Talbot Ingo Johannessen 《Journal of Cancer Therapy》 2012年第5期553-561,共9页
T cells engineered to express chimeric antigen receptors (CARs) combining an external antibody binding domain with the CD3ζ T cell receptor (TCR) signaling domain for triggering cell activation are being used for imm... T cells engineered to express chimeric antigen receptors (CARs) combining an external antibody binding domain with the CD3ζ T cell receptor (TCR) signaling domain for triggering cell activation are being used for immunotherapeutic targeting of tumor cells in a non-HLA restricted manner. In this study we transduced T cells with a CD19-CAR construct containing a truncated CD34 gene (tCD34) marker and used these to target the B cell antigen CD19 on the surface of a Hodgkin’s lymphoma (HL) cell line (L591) both in vitro and in vivo. Levels of tCD34 expression in transduced peripheral blood mononuclear cells (PBMCs) ranged from 6% - 20% and this was increased to 82% after selection for transduced tCD34+ cells. In vitro cytotoxicity testing on a CD19+ HL cell line (L591) showed specific cell lysis initiated by the CD19-CAR transduced PBMCs. Importantly, CD19-CAR T cells prevented the growth of L591 HL tumor cells when co-injected subcutaneously (sc) in 6/6 severe combined immunodeficient (SCID) mice. There was no evidence of anti-tumor activity when CD19-CAR T cells were infused intravenously (iv) at the same time as L591 HL tumor cells were injected sc. However, 3/6 SCID mice showed tumor rejection within 83 days after iv infusion of CD19-CAR T cells 3 - 9 days after establishment of L591 HL tumors, while all control animals succumbed to tumors within 60 days. Interestingly, immuno-histochemical analysis of L591 HL tumors demonstrated that CD19-CAR T cells were detected not earlier than 11 days after infusion within the tumor mass. These results suggest that CD19 is a potentially attractive target for the immunotherapy of HL. 展开更多
关键词 Hodgkin’s LYMPHOMA cd19 CHIMERIC antigen Receptor Immunotherapy
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Regulatory T cells suppress autoreactive CD4^+ T cell response to bladder epithelial antigen
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作者 Wu-Jiang Liu Yi Luo 《World Journal of Immunology》 2016年第2期105-118,共14页
AIM: To investigate the role of regulatory T (Treg) cells in CD4^+ T cell-mediated bladder autoimmune infammation. METHODS: Urothelium-ovalbumin (URO-OVA)/OT-II mice, a double transgenic line that expresses the... AIM: To investigate the role of regulatory T (Treg) cells in CD4^+ T cell-mediated bladder autoimmune infammation. METHODS: Urothelium-ovalbumin (URO-OVA)/OT-II mice, a double transgenic line that expresses the membrane form of the model antigen (Ag) OVA as a self-Ag on the urothelium and the OVA-specific CD4^+ T cell receptor specifc for the I-Ab/OVA323-339 epitope in the periphery, were developed to provide an autoimmune environment for investigation of the role of Treg cells in bladder autoimmune infammation. To facilitate Treg cell analysis, we further developed URO-OVA^GFP-Foxp3/OT-II mice, a derived line of URO-OVA/OT-II mice that express the green fuorescent protein (GFP)-forkhead box protein P3 (Foxp3) fusion protein. RESULTS: URO-OVA/OT-II mice failed to develop bladder infammation despite the presence of autoreactive CD4^+ T cells. By monitoring GFP-positive cells, bladder infltration of CD4^+ Treg cells was observed in URO-OVA^GFP-Foxp3/OT-II mice. The infiltrating Treg cells were functionally active and expressed Treg cell effector molecule as well as marker mRNAs including transforming growth factor-β, interleukin (IL)-10, fibrinogen-like protein 2, and glucocorticoid-induced tumor necrosis factor receptor (GITR). Studies further revealed that Treg cells from URO-OVA^GFP-Foxp3/OT-II mice were suppressive and inhibited autoreactive CD4^+ T cell proliferation and interferon (IFN)-g production in response to OVA Ag stimulation. Depletion of GITR-positive cells led to spontaneous development of bladder infammation and expression of inflammatory factor mRNAs for IFN-γ, IL-6, tumor necrosis factor-α and nerve growth factor in URO-OVA^GFP-Foxp3/OT-II mice. CONCLUSION: Treg cells specifc for bladder epithelial Ag play an important role in immunological homeostasis and the control of CD4^+ T cell-mediated bladder autoimmune infammation. 展开更多
关键词 BLADDER AUTOIMMUNITY Regulatory T cell cd4+ T cells antigen
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Functional expression of CD95/Fas Antigen and Bcl-2 on Cord blood Hematopoietic Progenitor Cells
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作者 MA Yanping(马艳萍) +2 位作者 ZOU Ping (邹萍) 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2002年第1期24-27,共4页
The cell surface expression and functional status of the CD95/Fas antigen on primitive hematopoietic progenitors isolated from human cord blood (CB) were studied. The CD34 + cells freshly isolated from CB displayed ... The cell surface expression and functional status of the CD95/Fas antigen on primitive hematopoietic progenitors isolated from human cord blood (CB) were studied. The CD34 + cells freshly isolated from CB displayed low CD95 expression. The combinations of cytokines such as SCF+FL could up regulate the expression of CD95 in vitro culture and tumor necrosis factor α (TNF α) and interon γ (IFN γ) further increased the CD95 expression induced by positive cytokines. The functional status of CD95 mediated apoptosis were analyzed by incubation of CD34 +CB cells in the presence of anti CD95 monoclonal antibodies (McAbs). The effects of anti CD95 McAbs were measured by viable cell counting, flow cytometry, LTIC and CFU C assays. A decrease of viable cells, CFU C and LTIC numbers were observed in the presence of anti CD95 McAbs and TNF α or IFN γ. However, growth factor deprivation or the early acting cytokine such as SCF and FL cross linking to CD95 caused low apoptosis of CD34 + cells. The correlation of increased intracytoplasmic levels of bcl 2 and the presence of CD95 on fresh CB CD34 + cells suggested that bcl 2 might be involved in protecting against CD95 mediated apoptosis of CB CD34 + cells. 展开更多
关键词 cd95/Fas Bcl 2 expression cord blood cd34 + cells
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Cinnamon extract suppresses experimental colitis through modulation of antigen-presenting cells 被引量:7
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作者 Ho-Keun Kwon Ji-Sun Hwang +8 位作者 Choong-Gu Lee Jae-Seon So Anupama Sahoo Chang-Rok Im Won Kyung Jeon Byoung Seob Ko Sung Haeng Lee Zee Yong Park Sin-Hyeog Im 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第8期976-986,共11页
AIM:To investigate the anti-inflammatory effects of cinnamon extract and elucidate its mechanisms for targeting the function of antigen presenting cells.METHODS:Cinnamon extract was used to treat murine macrophage cel... AIM:To investigate the anti-inflammatory effects of cinnamon extract and elucidate its mechanisms for targeting the function of antigen presenting cells.METHODS:Cinnamon extract was used to treat murine macrophage cell line(Raw 264.7),mouse primary antigen-presenting cells(APCs,MHCII+) and CD11c+dendritic cells to analyze the effects of cinnamon extract on APC function.The mechanisms of action of cinnamon extract on APCs were investigated by analyzing cytokine production,and expression of MHC antigens and co-stimulatory molecules by quantitative real-time PCR and flow cytometry.In addition,the effect of cinnamon extract on antigen presentation capacity and APC-dependent T-cell differentiation were analyzed by [H3]-thymidine incorporation and cytokine analysis,respectively.To confirm the anti-inflammatory effects of cinnamon extract in vivo,cinnamon or PBS was orally administered to mice for 20 d followed by induction of experimental colitis with 2,4,6 trinitrobenzenesulfonic acid.The protective effects of cinnamon extract against experimental colitis were measured by checking clinical symptoms,histological analysis and cytokine expression prof iles in inflamed tissue.RESULTS:Treatment with cinnamon extract inhibited maturation of MHCII+ APCs or CD11c+ dendritic cells(DCs) by suppressing expression of co-stimulatory molecules(B7.1,B7.2,ICOS-L),MHCII and cyclooxygenase(COX)-2.Cinnamon extract induced regulatory DCs(rDCs) that produce low levels of pro-inflammatory cytokines [interleukin(IL)-1β,IL-6,IL-12,interferon(IFN)-γ and tumor necrosis factor(TNF)-α] while expressing high levels of immunoregulatory cytokines(IL-10 and transforming growth factor-β).In addition,rDCs generated by cinnamon extract inhibited APC-dependent T-cell proliferation,and converted CD4+ T cells into IL-10high CD4+ T cells.Furthermore,oral administration of cinnamon extract inhibited development and progression of intestinal colitis by inhibiting expression of COX-2 and pro-inflammatory cytokines(IL-1β,IFN-γ and TNF-α),while enhancing IL-10 levels.CONCLUSION:Our study suggests the potential of cinnamon extract as an anti-inflammatory agent by targeting the generation of regulatory APCs and IL-10+ regulatory T cells. 展开更多
关键词 Cinnamon extract Inflammation cd4 antigen antigen presenting cells CYCLOOXYGENASE-2 Tumor necrosis factor-α INTERLEUKIN-10 Inflammatory bowel disease
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超声引导下粗针活检术联合CD34免疫组化标记在隆突性皮肤纤维肉瘤诊断中的应用
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作者 魏莉 孟宪杰 +1 位作者 洪蕾 王丽 《新疆医学》 2024年第4期459-461,469,共4页
目的探讨超声引导下粗针活检术联合CD34在隆突性皮肤纤维肉瘤的诊断中的应用价值。方法回顾性分析于术前经超声引导下粗针活检术及CD34免疫组化标记诊断为隆突性皮肤纤维肉瘤的32例患者的病理资料与超声检查结果,并将其与手术后病理结... 目的探讨超声引导下粗针活检术联合CD34在隆突性皮肤纤维肉瘤的诊断中的应用价值。方法回顾性分析于术前经超声引导下粗针活检术及CD34免疫组化标记诊断为隆突性皮肤纤维肉瘤的32例患者的病理资料与超声检查结果,并将其与手术后病理结果进行对比分析。结果32例患者中,30例患者于术前经超声引导下粗针活检术及CD34免疫组化标记诊断为隆突性皮肤纤维肉瘤,1例患者诊断为“倾向于隆突性皮肤纤维肉瘤”,另1例考虑“间叶源性肿瘤,不能除外纤维肉瘤”。术后病理结果提示31例患者为隆突性皮肤纤维肉瘤,1例患者为纤维肉瘤型皮肤纤维肉瘤。超声引导下粗针活检术联合CD34免疫组化标记诊断隆突性皮肤纤维肉瘤与术后病理结果的符合率为93.75%(30/31)。结论超声引导下粗针活检术联合CD34免疫组化标记诊断隆突性皮肤纤维肉瘤的准确性高,具有较大的临床应用价值。 展开更多
关键词 隆突性皮肤纤维肉瘤 超声引导下粗针穿刺术 超声检查 cd34 病理 临床
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CD34^(+)细胞数对单倍体造血干细胞移植治疗恶性血液病的影响
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作者 彭英楠 边志磊 +3 位作者 张素平 李丽 曹伟杰 万鼎铭 《中国组织工程研究》 CAS 北大核心 2024年第1期1-6,共6页
背景:单倍体造血干细胞移植与较高的植入功能不良相关,因此经常要求更高的CD34^(+)细胞数量,但现有研究关于异基因造血干细胞移植CD34+细胞剂量和研究终点关系的结论是有争议的。目的:探究CD34^(+)细胞数对单倍体造血干细胞移植治疗恶... 背景:单倍体造血干细胞移植与较高的植入功能不良相关,因此经常要求更高的CD34^(+)细胞数量,但现有研究关于异基因造血干细胞移植CD34+细胞剂量和研究终点关系的结论是有争议的。目的:探究CD34^(+)细胞数对单倍体造血干细胞移植治疗恶性血液疾病临床结果的影响。方法:纳入2019年1月至2021年12月期间于郑州大学第一附属医院造血干细胞移植中心行单倍体造血干细胞移植的恶性血液病患者,总计135例。结合既往研究结果及移植中心经验,以CD34+细胞数5.0×10^(6)/kg为截止点,将队列分为2组。评估两组的移植物植入情况、复发率及非复发死亡率、总生存期和无进展生存期等相关临床指标。结果与结论:①CD34+细胞剂量与血小板的植入相关,高剂量组血小板的植入时间早于低剂量组(14 d vs.16 d,P=0.013)。②两组患者3年总生存期无显著差异(67.5%vs.53.8%,P=0.257);两组间的无进展生存期也无显著性差异(65.6%vs.44.2%,P=0.106),但根据疾病风险指数(DRI)进行分层分析后发现低危患者高剂量组的3年无进展生存期较低剂量组升高(72.0%vs.49.3%,P=0.036)。③高剂量组3年累积复发率小于低剂量组(16.0%vs.33.5%,P=0.05)。④两组100 d内非复发死亡率高剂量组大于低剂量组,但无显著差异(17.3%vs.6.7%,P=0.070);进行分层分析发现,高危患者中高剂量组100 d内非复发死亡率明显高于低剂量组(20.0%vs.3.3%,P=0.046)。⑤综上所述,输注>5.0×10^(6)/kg的CD34^(+)细胞可促进血小板早期植入,可改善移植中低危风险患者的3年无进展生存期,并且降低移植后累积复发率;但在高危患者中,高剂量CD34+细胞导致移植后100 d内的非复发死亡率增高,考虑可能与移植后早期重度急性移植物抗宿主病的发生增多相关,因此考虑对回输高剂量CD34+细胞的患者应加强移植物抗宿主病的监测。 展开更多
关键词 cd34^(+)细胞 单倍体造血干细胞移植 恶性血液病 总生存 无进展生存 复发 非复发死亡
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浅表性CD34阳性纤维母细胞肿瘤6例临床病理分析及文献复习
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作者 刘四春 汤衡 +2 位作者 胡怀远 朱娜娜 胡向阳 《临床与实验病理学杂志》 CAS 北大核心 2024年第5期526-530,共5页
目的探讨浅表性CD34阳性纤维母细胞肿瘤(superficial CD34 positive fibroblastic tumors,SCPFT)的临床病理特点、免疫表型、诊断和鉴别诊断。方法回顾性分析6例SCPFT,行常规HE染色、免疫组化EliVision法染色。应用FISH法检测PRDM10基... 目的探讨浅表性CD34阳性纤维母细胞肿瘤(superficial CD34 positive fibroblastic tumors,SCPFT)的临床病理特点、免疫表型、诊断和鉴别诊断。方法回顾性分析6例SCPFT,行常规HE染色、免疫组化EliVision法染色。应用FISH法检测PRDM10基因断裂情况,并复习相关文献资料。结果6例SCPFT患者中,男性2例、女性4例。其中4例肿块位于下肢皮下,1例位于下腹部,另1例位于外阴。镜检:肿瘤细胞排列呈束状、实片状,细胞核明显多形性或畸形,核仁明显,可见核内假包涵体,胞质嗜酸性,部分区域细胞呈上皮样,未见核分裂象,间质散在少量炎细胞,免疫表型:肿瘤细胞弥漫强表达CD34和INI1,部分细胞表达CK(AE1/AE3),不表达SMA、CD68、desmin、S-100、CD31和ERG,Ki67增殖指数低于3%。FISH检测:4例SCPFT行FISH检测,其中3例PRDM10呈阳性。结论SCPFT是新近报道的一种软组织肿瘤,具有交界性或低度恶性生物学行为,诊断需与多种CD34阳性的软组织肿瘤相鉴别,避免过度诊断及治疗。 展开更多
关键词 纤维母细胞肿瘤 cd34 免疫组织化学 FISH检测
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The human leucocyte differentiation antigens (HLDA) workshops: the evolv-ing role of antibodies in research, diagnosis and therapy 被引量:2
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作者 Heddy ZOLA Bernadette SWART 《Cell Research》 SCIE CAS CSCD 2005年第9期691-694,共4页
The 8^th International Workshop on Human Leucocyte Differentiation Antigens (chaired by HZ and managed by BS) was run over a 4-year period and culminated in a conference in December 2004. Here we review the achievem... The 8^th International Workshop on Human Leucocyte Differentiation Antigens (chaired by HZ and managed by BS) was run over a 4-year period and culminated in a conference in December 2004. Here we review the achievements of the HLDA Workshops and provide links to information on CD molecules and antibodies against them, including the 93 new CDs assigned in the 8^th Workshop. We consider what remains to be achieved (including an estimate of the number of leucocyte surface molecules still to be discovered), and how the field can best move forward. 展开更多
关键词 leucocyte differentiation antigens cd molecules cell markers
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Bcl-2与CD34在基底细胞癌和毛发上皮瘤中的表达及意义
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作者 刘季 季珊维 《医药前沿》 2024年第9期144-146,共3页
目的:探讨B淋巴细胞瘤-2基因(Bcl-2)和集落分化抗原34(CD34)在基底细胞癌和毛发上皮瘤中的表达及意义。方法:选取2008年1月—2023年9月常熟市第五人民医院收治的基底细胞癌和毛发上皮瘤143例,根据不同疾病类型将患者分成毛发上皮瘤组(n=... 目的:探讨B淋巴细胞瘤-2基因(Bcl-2)和集落分化抗原34(CD34)在基底细胞癌和毛发上皮瘤中的表达及意义。方法:选取2008年1月—2023年9月常熟市第五人民医院收治的基底细胞癌和毛发上皮瘤143例,根据不同疾病类型将患者分成毛发上皮瘤组(n=85)和基底细胞癌组(n=58),对所有患者进行免疫组化检查,比较Bcl-2与CD34在不同疾病中的表达。结果:基底细胞癌组Bcl-2表达的阳性率为100.00%,CD34表达的阳性率为0.00%,毛发上皮瘤组Bcl-2表达的阳性率为89.41%,CD34表达的阳性率为13.48%,两组Bcl-2表达阳性率比较,差异有统计学意义(P<0.05)。Bcl-2在基底细胞癌诊断中的敏感度高于CD34,特异度低于CD34,差异有统计学意义(P<0.05);两种检查方式在基底细胞癌诊断中的准确率比较,差异无统计学意义(P>0.05)。Bcl-2在毛发上皮瘤诊断中的敏感度高于CD34,特异度低于CD34,差异有统计学意义(P<0.05);两种检查方式的准确率比较,差异无统计学意义(P>0.05)。结论:Bcl-2在基底细胞癌和毛发上皮瘤中呈高表达,在疾病的鉴别诊断中具有重要意义。CD34基底细胞癌中不表达,在毛发上皮瘤呈低表达,对于疾病的诊断和鉴别无显著意义。 展开更多
关键词 基底细胞癌 毛发上皮瘤 B淋巴细胞瘤-2基因 cd34 免疫组化
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Impact of PRRSV on activation and viability of antigen presenting cells 被引量:4
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作者 Irene M Rodríguez-Gómez Jaime Gómez-Laguna Librado Carrasco 《World Journal of Virology》 2013年第4期146-151,共6页
Porcine reproductive and respiratory syndrome(PRRS) is one of the most important diseases of swine industry. The causal agent, PRRS-virus(PRRSV), is able to evade the host immune response and survive in the organism c... Porcine reproductive and respiratory syndrome(PRRS) is one of the most important diseases of swine industry. The causal agent, PRRS-virus(PRRSV), is able to evade the host immune response and survive in the organism causing transient infections. Despite all scientific efforts, there are still some gaps in the knowledge of the pathogenesis of this disease. Antigen presenting cells(APCs), as initiators of the immune response, are located in the first line of defense against microorganisms, and are responsible for antigen recognition, processing and presentation. Dendritic cells(DCs) are the main type of APC involved in antigen presentation and they are susceptible to PRRSV infection. Thus, PRRSV replication in DCs may trigger off different mechanisms to impair the onset of a host effective immune response against the virus. On the one side, PRRSV may impair the basic functions of DCs by regulating the expression of major histocompatibility complex class Ⅱ and CD80/86. Other strategy followed by the virus is the induction of cell death of APCs by apoptosis, necrosis or both of them. The impairment and/or cell death ofAPCs could lead to a failure in the onset of an efficient immune response, as long as cells could not properly activate T cells. Future aspects to take into account are also discussed in this review. 展开更多
关键词 Porcine REPRODUCTIVE and respiratory syndrome antigen PRESENTING CELLS DENDRITIC CELLS Immune response Major HISTOCOMPATIBILITY complex classⅡ cd80/86 Cell death Apoptosis
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Key role of human leukocyte antigen in modulating human immunodeficiency virus progression: An overview of the possible applications 被引量:1
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作者 Alba Grifoni Carla Montesano +1 位作者 Vittorio Colizzi Massimo Amicosante 《World Journal of Virology》 2015年第2期124-133,共10页
Host and viral factors deeply influence the human immunodeficiency virus(HIV) disease progression. Among them human leukocyte antigen(HLA) locus plays a key role at different levels. In fact, genes of the HLA locus ha... Host and viral factors deeply influence the human immunodeficiency virus(HIV) disease progression. Among them human leukocyte antigen(HLA) locus plays a key role at different levels. In fact, genes of the HLA locus have shown the peculiar capability to modulate both innate and adaptive immune responses. In particular, HLA class Ⅰmolecules are recognized by CD8+ T-cells and natural killers(NK) cells towards the interaction with T cell receptor(TCR) and Killer Immunoglobulin Receptor(KIR) 3DL1 respectively. Polymorphisms within the different HLA alleles generate structural changes in HLA classⅠpeptide-binding pockets. Amino acid changes in the peptide-binding pocket lead to the presentation of a different set of peptides to T and NK cells. This review summarizes the role of HLA in HIV progression toward acquired immunodeficiency disease syndrome and its receptors. Recently, many studies have been focused on determining the HLA binding-peptides. The novel use of immune-informatics tools, from the prediction of the HLA-bound peptides to the modification of the HLAreceptor complexes, is considered. A better knowledge of HLA peptide presentation and recognition are allowing new strategies for immune response manipulation to be applied against HIV virus. 展开更多
关键词 HUMAN IMMUNODEFICIENCY virus PROGRESSION HUMAN LEUKOCYTE antigen EPITOPE IMMUNOINFORMATICS cd8+T lymphocytes
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Long Non-coding RNA PCED1B Antisense RNA 1 Promotes Cell Proliferation and Invasion in Hepatocellular Carcinoma by Regulating the MicroRNA-34a/CD44 Axis
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作者 Jian-gang BI Qi LI +3 位作者 Yu-sheng GUO Li-ping LIU Shi-yun BAO Ping XU 《Current Medical Science》 SCIE CAS 2024年第3期503-511,共9页
Objective This study aimed to examine the role of long non-coding RNA PCED1B antisense RNA 1(PCED1B-AS1)in the development of hepatocellular carcinoma(HCC).Methods A total of 62 pairs of HCC tissues and adjacent non-t... Objective This study aimed to examine the role of long non-coding RNA PCED1B antisense RNA 1(PCED1B-AS1)in the development of hepatocellular carcinoma(HCC).Methods A total of 62 pairs of HCC tissues and adjacent non-tumor tissues were obtained from 62 HCC patients.The interactions of PCED1B-AS1 and microRNA-34a(miR-34a)were detected by dual luciferase activity assay and RNA pull-down assay.The RNA expression levels of PCED1B-AS1,miR-34a and CD44 were detected by RT-qPCR,and the protein expression level of CD44 was determined by Western blotting.The cell proliferation was detected by cell proliferation assay,and the cell invasion and migration by transwell invasion assay.The HCC tumor growth after PCED1B-AS1 was downregulated was determined by in vivo animal study.Results PCED1B-AS1 was highly expressed in HCC tissues,which was associated with poor survival of HCC patients.Furthermore,PCED1B-AS1 interacted with miR-34a in HCC cells,but they did not regulate the expression of each other.Additionally,PCED1B-AS1 increased the expression level of CD44,which was targeted by miR-34a.The cell proliferation and invasion assay revealed that miR-34a inhibited the proliferation and invasion of HCC in vitro,while CD44 exhibited the opposite effects.Furthermore,PCED1B-AS1 suppressed the role of miR-34a.Moreover,the knockdown of PCED1B-AS1 repressed the HCC tumor growth in nude mice in vivo.Conclusion PCED1B-AS1 may play an oncogenic role by regulating the miR-34a/CD44 axis in HCC. 展开更多
关键词 long non-coding RNA PCED1B antisense RNA 1(PCED1B-AS1) hepatocellular carcinoma microRNA-34a(miR-34a) cd44 proliferation INVASION
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Studies on mechanism of Sialy Lewis-X antigen in liver metastases of human colorectal carcinoma 被引量:19
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作者 Xiao Wei Li~1 Yan Qing Ding~1 Jun Jie Cai~1 Shao Qing Yang~2 Lian Bing An~3 Dong Fang Qiao~3 ~1Department of Pathology,Nanfang Hospital of the First Military Medical University,Guangzhou 510515,Guangdong Province,China ~2The Northern Hospital of PLA,Shenyang 110015,Liaoning Province,China ~3Department of Electronmicroscopy,First Military Medical University,Guangzhou 510515,Gangdong Province,ChinaDr.Xiao Wei Li graduated from the First Military Medical University with a MM degree in 1999.Physician in Charge of pathology,having 6 papers published. 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第3期425-430,共6页
INTRODUCTIONSialyl Lewis-X antigen ,correlated with carcinoma, is a group of carbohydrate antigen containing oligosaccharide expressed of embryonic tisue and glycoproteins on cell surface of embryonic tissue[1].The SL... INTRODUCTIONSialyl Lewis-X antigen ,correlated with carcinoma, is a group of carbohydrate antigen containing oligosaccharide expressed of embryonic tisue and glycoproteins on cell surface of embryonic tissue[1].The SLeX antigen located on cell surface is synthesized principally by two enzymes ,al ,3fucosyltransfrease and a2, 3sialyctransferase.In adults ,SLeX antigen is expressed principally on the surfaces of granulocytic cells and some tumor cells . 展开更多
关键词 Animals Antibodies Monoclonal antigens cd15 Cell Adhesion Colorectal Neoplasms E-Selectin Endothelium Vascular Flow Cytometry HT29 Cells Humans Immunohistochemistry In Situ Hybridization Liver Neoplasms MICE Mice Inbred BALB C Mice Nude Microscopy Electron Microscopy Electron Scanning N-Acetylneuraminic Acid RNA Messenger Research Support Non-U.S. Gov't Tumor Cells Cultured Umbilical Veins
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The Secondary Structure of Heated Whey Protein andIts Hydrolysates Antigenicity
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作者 PANG Zhi-hua ZHU Jun +4 位作者 WU Wei-jing WANG Fang REN Fa-zheng ZHANG Lu-da GUO Hui-yuan 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2011年第11期3055-3059,共5页
Fourier transform infrared spectroscopy(FTIR) and circular dichroism(CD) were used to investigate the conformational changes of heated whey protein(WP) and the corresponding changes in the hydrolysates immunoreactivit... Fourier transform infrared spectroscopy(FTIR) and circular dichroism(CD) were used to investigate the conformational changes of heated whey protein(WP) and the corresponding changes in the hydrolysates immunoreactivity were determined by competitive enzyme-linked immunosorbent assay(ELISA).Results showed that the contents of α-helix and β-sheet of WP did not decrease much under mild heating conditions and the antigenicity was relatively high;when the heating intensity increased(70 ℃ for 25 min or 75 ℃ for 20 min),the content of α-helix and β-sheet decreased to the minimum,so was the antigenicity;However,when the WP was heated at even higher temperature and for a longer time,the β-sheet associated with protein aggregation begun to increase and the antigenicity increased correspondingly.It was concluded that the conformations of heated WP and the antigenicity of its hydrolysates are related and the optimum structure for decreasing the hydrolysates antigeniity is the least content of α-helix and β-sheet.Establishing the relationship between the WP secondary structure and WP hydrolysates antigenicity is significant to supply the reference for antigenicity reduction by enzymolysis. 展开更多
关键词 FTIR cd Whey protein Heat Treatment antigenICITY
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CD34和S100共表达的ALK重排皮肤梭形细胞肿瘤2例及文献复习
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作者 安晋 李丽 《中国临床新医学》 2024年第7期745-748,共4页
目的报道2例具有独特形态学特征、CD34和S100共表达的间变性淋巴瘤激酶(ALK)重排皮肤梭形细胞肿瘤,以提高临床医师对该类软组织梭形细胞肿瘤的认识。方法收集2例外院会诊的病例,采用免疫组化EnVision二步法检测肿瘤中vimentin、ALK(D5F3... 目的报道2例具有独特形态学特征、CD34和S100共表达的间变性淋巴瘤激酶(ALK)重排皮肤梭形细胞肿瘤,以提高临床医师对该类软组织梭形细胞肿瘤的认识。方法收集2例外院会诊的病例,采用免疫组化EnVision二步法检测肿瘤中vimentin、ALK(D5F3)、BCL2、CD34及Ki-67的表达情况。1例使用荧光原位杂交(FISH)法断裂探针、1例通过二代测序(NGS)检测ALK基因重排。结果形态学上,肿瘤细胞由温和的梭形细胞或卵圆形细胞构成,呈束状或漩涡状排列,间质玻璃样变及黏液变性,可见较多开放的血管腔。该类肿瘤同时表达CD34和S100,存在ALK基因重排。结论2例CD34和S100共表达的ALK重排皮肤梭形细胞肿瘤具有独特的形态学,具有相同的免疫组化表达及分子特征,与NTRK重排梭形细胞肿瘤具有相似的临床病理学特征。 展开更多
关键词 ALK重排 cd34和S100共表达 皮肤梭形细胞肿瘤
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高频彩超结合血清CD34、Tg、VEGF诊断甲状腺癌的临床价值
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作者 杨林靖 《医疗装备》 2024年第7期94-96,共3页
目的分析高频彩超结合血清细胞表面的唾液黏蛋白(CD)34、甲状腺球蛋白(Tg)、血管内皮生成因子(VEGF)诊断甲状腺癌的临床价值。方法选取2018年1月至2023年10月医院收治的50例疑似甲状腺癌患者,均采用高频彩超诊断,并检测Tg、VEGF、CD34... 目的分析高频彩超结合血清细胞表面的唾液黏蛋白(CD)34、甲状腺球蛋白(Tg)、血管内皮生成因子(VEGF)诊断甲状腺癌的临床价值。方法选取2018年1月至2023年10月医院收治的50例疑似甲状腺癌患者,均采用高频彩超诊断,并检测Tg、VEGF、CD34表达。以手术病理结果为金标准,分为良性组与恶性组,分析对甲状腺癌的诊断价值。结果恶性组收缩期峰值流速(PSV)、阻力指数(RI)高于良性组,血流分级3级占比率高于良性组,纵横比>1、内部微钙化、后方衰减占比率均高于良性组(P<0.05)。恶性组血清Tg、VEGF、CD34表达水平均高于良性组(P<0.05)。绘制受试者工作特征曲线(ROC)后,高频彩超结合血清Tg、VEGF、CD34预测甲状腺癌的灵敏度(93.30%)、特异度(92.50%)均高于单一指标(P<0.05)。结论高频彩超结合血清CD34、Tg、VEGF表达诊断甲状腺癌的临床价值较高。 展开更多
关键词 高频彩超 cd34 TG VEGF 甲状腺癌
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CD34和D2-40在原发性结肠腺癌中的表达及与伴淋巴结转移状态的关系
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作者 赵学影 侯仕璇 +1 位作者 许远霞 陆进 《包头医学院学报》 CAS 2024年第10期19-22,50,共5页
目的:探讨原发性结肠腺癌(colon adenocarcinoma,COAD)组织中血管和淋巴管标志物CD34(CD34 Molecule)和D2-40(又称Podoplanin)标记的癌胚抗原M2A的表达及其意义。方法:采用免疫组织化学Elivision TM plus法检测60例COAD组织和4例癌旁正... 目的:探讨原发性结肠腺癌(colon adenocarcinoma,COAD)组织中血管和淋巴管标志物CD34(CD34 Molecule)和D2-40(又称Podoplanin)标记的癌胚抗原M2A的表达及其意义。方法:采用免疫组织化学Elivision TM plus法检测60例COAD组织和4例癌旁正常组织中CD34与D2-40标记的M2A蛋白表达,计数CD34阳性的微血管密度和D2-40阳性的微淋巴管密度,并分析其与COAD临床病理参数的关系。结果:在COAD组织中,CD34、D2-40阳性的微血管密度和微淋巴管密度高于癌旁正常组织(P<0.05)。COAD组织中的CD34微血管密度与病变部位有关,右半结肠癌患者中CD34微血管密度明显高于左半结肠癌患者(P<0.05);伴肠系膜淋巴结转移的COAD组织中CD34微血管密度与D2-40微淋巴管密度呈正相关(P<0.05)。结论:CD34和D2-40标记M2A蛋白在COAD组织中高表达,并且在伴肠系膜淋巴结转移状态下二者呈正相关,提示它们可能协同参与COAD淋巴结转移的发生。 展开更多
关键词 结肠腺癌 微血管密度 微淋巴管密度 cd34 D2-40
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