Immobilization of antibodies on the self-assembled monolayer by antigen-binding site protection and immobilization kinetic control

The orientation of the biological molecule immobi-lized on a solid surface has been critical in devel-opment of various applications. In this study, ori-entation of antibody was retained by protecting the antigen-binding site of the antibody prior to immo-bilization to -functionalized mixed self-assembled monolayer (SAM) of 12-mercaptododecanoic acid and 1-heptanethiol. More importantly, the number of immobilization bonds formed between each an-tigen-binding site protected antibody molecule and the solid surface was controlled by optimizing the mole fraction of the activated carboxyl group of the linker molecules in the mixed SAM. The amount of antibody used in this study was approximately equivalent to the amount for one monolayer surface coverage. The resulting activity of protected immo-bilized antibody was about 10 fold higher than that of random immobilized

Evaluation of the Protection and Utilization of Great Sites:A Case Study of Zhengzhou City

The protection and utilization of great sites not only are related to the inheritance of historical and cultural heritage and academic research,but also directly affect local economic development and social and cultural dissemination.In this paper,five major great sites in Zhengzhou City(Dahe Village Site,Zhengzhou Shangcheng Site,Xiaoshuangqiao Site,the Capital of Zheng and Han States,and Mausoleum of Song Dynasty Tombs of Song Emperors)are taken as research objects,and a set of index system for evaluating the protection and utilization of great sites is constructed.A comprehensive evaluation is conducted from seven aspects:protection management,research value,site utilization,cultural value dissemination,socio-economic benefits,utilization effects,and regional development coordination.This paper provides a comprehensive and scientific evaluation perspective for the protection and utilization of great sites,which helps to better grasp the direction of site protection and utilization,and develop more practical and effective strategies for site protection and utilization.

Study on Mosuo's Sacred Natural Sites of Mountainous Forest and Protection of Biological Diversity in Yongning-Lugu Lake Area

With Mosuo's sacred natural sites of mountainous forest in Yongning-Lugu Lake area as the research subject,by using the method of ecology,the vegetation type in this area was initially identified.The control sample plot of the same vegetation type at similar altitude and in similar soil condition would be selected and compared with the sample plot in sacred natural sites at length,so as to quantitatively analyze the protection of biological diversity in the perspective of ecological system.It showed that under the circumstance of the damage of ecological environment,complete natural vertical pattern was preserved thanks to sacred natural sites of mountainous forest established by reason of traditional religion and culture.The results of research and quantitative analysis showed that compared with the control sample plot of the same vegetation type,sacred natural sites were high in biological diversity and the species composition of plant community in sacred natural sites was closer to that of primitive forest.The result of quantitative analysis also indicated that sacred natural sites played a remarkable role in protecting vegetation and this protective function became more remarkable if the altitude was higher.And human were reminded to pay close attention to the preservation and protection function of sacred natural sites to natural ecological system at high altitude.

Some Thoughts on the Protection and Display of Yuanmingyuan Site

Via field survey and analysis,the problems that authenticity of Yuanmingyuan site protection is nibbled by modern garden engineering,and Yuanmingyuan site display has low identification degree,insufficient historical information and improper construction site piece display are elaborated. Meanwhile,it is proposed locating Yuanmingyuan site protection and display into the residual landscape after burnt and looted in 1860. Virtual reality technology is used for full-landscape coverage,to realize the comparison between history and status quo,and the dissemination of Yuanmingyuan information is expanded by"internet+"model,which aims to impel the public joining in heritage awareness and cultural relic preservation.

The Principle of Authenticity and the Protection and Renewal of the Qujiang Site in Ancient Chang'an

In order to explore the protection and renewal law of historic sites in the process of contemporary urban construction, combined with the protection and renewal practice of Qujiang garden cultural landscape heritage sites in ancient Chang'an, this paper analyzed the authenticity in the protection and renewal of different historic sites, and explored the possibility of flexible use of the concept of authenticity in the process of protection and renewal of historic sites.

Temporal and Spatial Differentiation of Cultural Heritage in Jingzhou Area of National Great Site Protection and Its Influencing Factors

In the context of territorial spatial planning,the overall coordinated protection has become a consensus in the field of cultural heritage protection,and the study on the spatial and temporal pattern of trans-regional cultural heritage is of great significance to the overall protection.Based on the cultural relics protection units in Jingzhou area of national great site protection,the temporal and spatial distribution characteristics and influencing factors of cultural heritage resources were studied with the help of ArcGIS.The data show that on the whole,the cultural heritage in Jingzhou area of national great site protection presented the time series of “a large span,more at two ends” and the spatial distribution pattern of “one main,two secondary and three cores”.Cultural heritage in different historical periods had the characteristics of differentiated regional aggregation.The formation of the spatial pattern of different types of cultural heritage was closely related to the local historical and cultural background.Cultural heritage was affected by rivers,terrain,transportation,land,culture and other factors.It was mainly distributed along rivers in areas with little topographic fluctuation and low altitudes.The development and construction of cultural heritage sites were weak,and the ecological foundation was poor.However,the traffic accessibility was good,and the cultural heritage was deep.The research results are expected to provide a scientific basis for guiding the large site protection area to strengthen the integrated protection of cultural resources.

The Status Quo and Problems of Red Cultural Sites in the New Era and Their Exploration Paths: Taking the Luxi Region as an Example

Red cultural site is the carrier of red culture,containing profound revolutionary history and value of the times,we should deeply excavate the intrinsic potential of red cultural sites,pay attention to the ontological protection of red cultural sites,develop and improve the red cultural innovation projects,and strengthen the red culture itself and supporting the construction.This paper takes the old revolutionary area of Luxi as an example,conducts field investigation and visits based on the local county records and party history materials,and investigates a total of five red cultural sites and memorial halls in Luxi,focusing on the actual achievements and problems exposed in the protection and development of the current red cultural sites,to provide references and thinking for the protection,development,and innovative utilization of red cultural sites in the new era of our country.

Protection of Environment from Damaged Nuclear Station and Transparent Inflatable Blanket for Cities—Protection from Radioactive Dust and Chemical, Biological Weapons

The author, in a series of previous articles, designed the “AB Dome” made of transparent thin film supported by a small additional air overpressure for the purpose of covering a city or other important large installations or sub-regions. In present article the author offers a variation in which a damaged nuclear station can be quickly covered by such a cheap inflatable dome. By containing the radioactive dust from the damaged nuclear station, the danger zone is reduced to about 2 km2 rather than large regions which requires the resettlement of huge masses of people and which stops indus-try in large areas. If there is a big city (as Tokyo) near the nuclear disaster or there is already a dangerous amount of radioactive dust near a city, the city may also be covered by a large inflatable transparent Dome. The building of a gi-gantic inflatable AB Dome over an empty flat surface is not difficult. The cover is spread on a flat surface and a venti-lator (fan system) pumps air under the film cover and lifts the new dome into place but inflation takes many hours. However, to cover a city, garden, forest or other obstacle course in contrast to an empty, mowed field, the thin film cannot be easily deployed over building or trees without risking damage to it by snagging and other complications. This article proposes a new method which solves this problem. The design is a double film blanket filled by light gas such as, methane, hydrogen, or helium - although of these, methane will be the most practical and least likely to leak. Sections of this AB Blanket are lighter than air and will rise in the atmosphere. They can be made on a flat area serving as an as-sembly area and delivered by dirigible or helicopter to station at altitude over the city. Here they connect to the already assembled AB Blanket subassemblies, cover the city in an AB Dome and protect it from bad weather, chemical, bio-logical and radioactive fallout or particulates. After assembly of the dome is completed, the light gas can be replaced by (heavier but cheaper) air. Two projects for Tokyo (Japan) and Moscow (Russia) are used in this paper for sample computation.

SITE CLASSIFICATION FOR PROTECTIVE FOREST AFFORESTATION ON THE COAST OF CHINA

Land for protective forest on the coast has special site conditions, and site classification is the scientific basis for seaboard afforestation. The site classification system on the coast zone and islands of China may be classified into five levels-site region (sub - region), district, class, group, and type. The land division for afforestation is carried out by the principle of enviornmental heterogeneity among regions, sub-region and district on large scale, according to the difference of air temperature, moisture and type of coast geomorphy. It may be classified into 7 regions, 12 sub-regions and 55 districts. The medium and small scaled division for site class, group and type, subdivided in a site district, are based on medium topography, topographic climate, micro - relief and soil conditions.

Fresh Efforts to Protect Peking Man Site

CAS scientists have worked out a plan to further protect the Peking Man site at Zhoukoudian (ZKD) in Beijing, and to renovate research facilities at this internationally renowned paleoanthropological and paleolithic location.……

乡村古建筑史迹数字化保护设计

该文从乡村古建筑史迹数字化发展面临的问题出发,选取桂林全州县屏山渡红军古街作为研究对象,通过实地考察收集到的图像和数据,构建出三维数字场景,再现当地建筑环境,达到对乡村古建筑史迹数字化保护的目的。通过数字化的方式,吸引更多的人关注乡村保护,实现文化遗产的传承,同时也对于未来的发展提出一些想法和期望。

良渚古城老虎岭水坝遗址考古剖面保护研究与工程实践

良渚古城老虎岭水坝遗址是我国迄今发现最早的大型水利工程系统中的一处典型遗存,对中国文明起源和世界文明史的研究具有重要意义。为清晰地展示老虎岭水坝土遗址上“草裹泥”筑坝工艺的痕迹,针对温热、潮湿、多雨环境土遗址保护的难题,在总结吸取国内土遗址保护经验教训的基础上,借助浙江大学的研究成果和敦煌研究院的工程经验,提出了一套避免使用化学材料加固,而以“黑屋+隔水+控湿”为主的小环境控制工程策略,包括:构建遮光大棚以避雨和抑制绿色生物生长;铺设防水毯以防止坝体渗水;设置电加热玻璃罩以保湿和封闭展示面;采用气相补水以调控空气湿度和土体含水率;采用植物精油等绿色环保技术防治微生物;同时做好遗址实时监测和管护等。这套综合保护措施是土遗址保护理念和工程实践的一次新的尝试。

CTCS-2+ATO列控系统对短站坪长度需求适应性研究

研究目的:对于有地下线路的铁路工程,由于开挖土方等方面的问题,要求站坪长度尽量缩短,以节省工程投资。现有CTCS-2+ATO列控系统因其安全防护距离设置位置、车载设备安装位置、适应的运行速度较高等原因,对站坪长度要求较CBTC列控系统长,成为在市域铁路运用时的不利因素,需要对其进行短站坪长度需求的适应性分析并提出适宜的解决方法,提升系统的竞争力。研究结论:(1)侧线股道增设安全线,基于列车停稳信息及时解锁过走防护区段、利用应答器报文增加过走防护区段信息、及时回缩列控移动授权范围等配套方法,将安全距离、附加距离设于目标点信号机的内方,利用安全线及咽喉区线路缩短股道区线路长度;(2)合理约束车载应答器接收天线安装位置,根据市域列车运行速度计算、确定可靠解析出报文所需要的应答器距离信号机距离,以及应答器组间最小距离要求,最终确定股道出站应答器安装位置最小长度需求;(3)经折返线道岔侧向的进路不发低频码,轨道电路长度仅考虑轨道占用检查时间,进一步缩短折返道岔区段长度要求;(4)本研究成果在市域铁路中有广阔的运用前景。

世界遗产地保护传承导向的镇域国土空间规划探索

自北京总规批复以来,国土空间规划按照三级三类四体系的整体框架层层推进,镇域国土空间规划作为“中腰”部分,重在对控制要求的上传下达,做好各类指标和空间管控要素的刚性传导.北京历史悠久,文化资源丰富,是全国文化中心,如何保护好北京历史文化“金名片”,将历史文化保护的要求更好地传导到镇域国土空间规划中,又能向下贯彻落实,指引城乡发展建设,让历史文化遗产能够保得住、用得好是一个值得研究的课题.在分析世界遗产地面临的核心挑战的基础上,提出镇域国土空间规划应转变认知,协调好“遗产与区域、文物与本底、文化与空间、保护与发展”四组关系,并以世界文化遗产保护与乡镇发展共荣的十三陵实践为例,搭建起“整体认知-格局引领-分层保护-传承发展”的国土空间规划编制技术框架,以期能给相关工作提供有益借鉴.

郑州国棉三厂纺织工业遗产保护与再生研析

中国近代纺织工业是洋务运动实现“自强求富”、民族资本“实业救国”的重要工业类型之一,是中国近代工业化的重要内容,在中国近代工业史上具有十分重要的意义。中华人民共和国成立初期的6大纺织工业基地中,郑州国棉三厂是其中规模较大、质量较高、延续时间较长的工业厂区。基于工业遗产病害分析、结构评估和博物馆展示利用需求,采取文物建筑修缮加固、文物建筑风貌恢复、展览空间活化利用、新旧建筑融合共生的策略,实现工业建筑遗产向工业遗址博物馆的活化利用,为近代纺织工业遗产的保护与再生提供借鉴。

红色遗址数字化保护现状及应用策略研究——以吉林省为例

梳理国内数字化政策,总结国内红色遗址保护现状,探索数字化技术在红色遗址保护中的发展方向,为吉林省红色遗址提供新的保护方法与途径。通过搜索整理中国知网(CNKI)的学术期刊文献,并借助CiteSpace进行可视化分析,得出2010—2023年红色遗址保护现状及研究趋势。研究发现传统的保护手段具有诸多弊端,需要数字化技术的介入提供红色遗址保护的新思路,当前数字化技术正逐渐应用于红色遗址的开发利用、设计施工、展示宣传过程中,成为未来发展的新趋势,为吉林省红色遗址保护提供参考。

基于本体的欧洲水利遗产信息资源组织研究

欧洲水利遗产丰富,为从中获取水利遗产历史信息组织知识和经验,促进我国水利遗产的系统性保护和传承创新,有必要系统梳理和组织欧洲水利遗产信息资源。采用情报学理论与方法分析了欧洲水利遗产现状,结合信息技术发展趋势构建了欧洲水利遗产数字化保护信息技术方法体系,借助本体技术设计欧洲水利遗产信息资源组织过程,并提出了欧洲水利遗产信息资源组织合作管理平台的实现思路。研究结果表明:提出的组织框架有利于欧洲水利遗产数字化保护,随着信息技术发展,能够实现欧洲水利遗产信息资源共享应用。

燎祭对三星堆古象牙周际土壤微生物群落的影响

三星堆遗址四号祭祀坑象牙表面留有灼烧痕迹,其上附着黑色灰烬.为了解黑色灰烬土对古象牙保存的影响,本研究比较了灰烬土和对照黄土的理化性质,并对其中微生物进行了高通量测序和多组学分析.结果发现,灰烬土中水分和有机碳含量显著高于未灼烧黄土(P<0.05).高通量测序显示,灰烬土和黄土中均以变形菌门(Proteobacteria)和担子菌门(Basidiomycota)占优势,灰烬土中含有更高丰度的细菌属Methylotenera、Flavobacterium和Ac⁃idovorax,以及真菌属Mortierella、Penicillium和Aspergillus.代谢组显示,灰烬土中L-苹果酸、泛酸等有机酸显著低于黄土,而对古象牙腐蚀性更强的草酸则显著高于黄土(P<0.05).因此,烧灼引起土壤理化性质的显著变化,使灰烬土中含有更高水分和有机碳,以及更低pH值,其微生物群落结构和组成改变导致草酸增高,增加了出土古象牙的腐蚀风险.

“公园城市”视角下遗址及其周边区域更新建设策略

伴随我国“十三五”规划的圆满完成,对遗址及其周边空间的保护利用迈入全新阶段。但从我国大遗址保护利用现状来看,仍有一些问题亟待解决,“公园城市”所蕴含的部分原则与方法正是解决此类问题的有效手段。文章结合大遗址保护理念、“公园城市”理念及相关实践案例,总结出了整体统一、生态优先、文化为核、以人为本四条基本更新建设原则。从上述四条原则出发对遗址保护区域提出对应的绿化形式与展示手法,对遗址周边区域从地形、水系、植物、建筑、道路及设施六个方面出发,提出针对性的景观更新建设策略。以期对同类空间起到借鉴作用,使得遗址及其周边区域得以充分展现自身风韵,更好地服务居民生活,助力城市高效发展。

仪表着陆系统下滑信标运行保护区仿真研究

仪表着陆系统下滑信标运行保护区的科学防护,对于确保民用航空导航空间信号质量、保障航空器进近阶段运行安全、提升机场运行效率均具有重要意义。结合下滑信标运行保护区概念的演进,对民用航空国家标准、行业标准,以及国际民用航空公约附件十第I卷要求的下滑信标运行保护区的划设情况进行了对比分析。以特大型航空器A380-800为例,对特定运行类别的下滑信标运行保护区进行了研究,以期作为国际民用航空公约附件十第I卷关于下滑信标保护区相关数据的补充和实际工程论证的示例。给出了针对特定机场实际运行进行下滑信标场地保护的典型研究方法以及下滑信标运行保护区的保护建议,为机场的建设和运行提供参考。