Excessive free radicals can cause oxidative stress (OS),which lead to neurodegenerative diseases (ND) are harmful to the human body.Therefore,it is necessary to explore dietary antioxidants and neuroprotectants from n...Excessive free radicals can cause oxidative stress (OS),which lead to neurodegenerative diseases (ND) are harmful to the human body.Therefore,it is necessary to explore dietary antioxidants and neuroprotectants from nutrient foods.Chaga mushroom (Inonotus obliquus ) has been used as an important functional food in many countries for centuries.This present study was carried out to discover active compounds from I.obliquus and investigate the in vitro antioxidant activity and neuroprotective activity.Sixteen natural products including two new isocoumarins (1 –2 ),a new 8-O -4′-neolignan (3 ),a new cyclic diarylheptanoid (4 ),and twelve known compounds (5 –16 ) were separated from I.obliquus .Their structures were elucidated by extensive spectroscopic analyses of high resolution electrospray mass spectrometry (HRESIMS),ultra violet (UV),and nuclear magnetic resonance (NMR).Among them,compounds 1 and 2 revealed remarkable antioxidant activities through the comprehensive analysis of DPPH,ABTS,and ferric reducing antioxidant power (FRAP) methods.Meanwhile,compound 1 demonstrated significant neuroprotective activity by increasing SH-SY5Y cells viability and preventing mitochondrial damage.Apart from that,electronic analyses were performed using the highest occupied molecular orbital (HOMO),and the lowest unoccupied molecular orbital (LUMO) to analyze compound 1 .These results indicated that compound 1 had a significant implication for the search of natural raw material to prevent OS and nerve damage in the field of functional foods,and the isocoumarin fragment as well as an oxygenated cis double-bond might be the active sites to play prominent parts in antioxidant and neuroprotective activities of compound 1 .展开更多
以桦褐孔菌子实体为材料,用不同溶剂对其进行分步提取,确定有效的提取工艺并对得到的提取物进行生物活性鉴定,生物活性主要是抗菌性和抗氧化性。结果显示,对乙醇浸膏进行萃取时,依次分步加入石油醚、乙酸乙酯、正丁醇150~200 m L得到萃...以桦褐孔菌子实体为材料,用不同溶剂对其进行分步提取,确定有效的提取工艺并对得到的提取物进行生物活性鉴定,生物活性主要是抗菌性和抗氧化性。结果显示,对乙醇浸膏进行萃取时,依次分步加入石油醚、乙酸乙酯、正丁醇150~200 m L得到萃取液,控制旋蒸、烘干温度在50℃以下,即可得到有生物活性的乙酸乙酯提取物和正丁醇提取物。乙酸乙酯提取物和正丁醇提取物的提取率约为0.1%。展开更多
基金supported by China Postdoctoral Science Foundation(2020M680987)Science and Technology Planning Project of Liaoning Province(2021JH1/10400049)+1 种基金Career Development Support Plan for Young and Middle-aged Teachers in Shenyang Pharmaceutical University(ZQN2018006)the Project of Innovation Team Foundation(LT2015027).
文摘Excessive free radicals can cause oxidative stress (OS),which lead to neurodegenerative diseases (ND) are harmful to the human body.Therefore,it is necessary to explore dietary antioxidants and neuroprotectants from nutrient foods.Chaga mushroom (Inonotus obliquus ) has been used as an important functional food in many countries for centuries.This present study was carried out to discover active compounds from I.obliquus and investigate the in vitro antioxidant activity and neuroprotective activity.Sixteen natural products including two new isocoumarins (1 –2 ),a new 8-O -4′-neolignan (3 ),a new cyclic diarylheptanoid (4 ),and twelve known compounds (5 –16 ) were separated from I.obliquus .Their structures were elucidated by extensive spectroscopic analyses of high resolution electrospray mass spectrometry (HRESIMS),ultra violet (UV),and nuclear magnetic resonance (NMR).Among them,compounds 1 and 2 revealed remarkable antioxidant activities through the comprehensive analysis of DPPH,ABTS,and ferric reducing antioxidant power (FRAP) methods.Meanwhile,compound 1 demonstrated significant neuroprotective activity by increasing SH-SY5Y cells viability and preventing mitochondrial damage.Apart from that,electronic analyses were performed using the highest occupied molecular orbital (HOMO),and the lowest unoccupied molecular orbital (LUMO) to analyze compound 1 .These results indicated that compound 1 had a significant implication for the search of natural raw material to prevent OS and nerve damage in the field of functional foods,and the isocoumarin fragment as well as an oxygenated cis double-bond might be the active sites to play prominent parts in antioxidant and neuroprotective activities of compound 1 .
文摘以桦褐孔菌子实体为材料,用不同溶剂对其进行分步提取,确定有效的提取工艺并对得到的提取物进行生物活性鉴定,生物活性主要是抗菌性和抗氧化性。结果显示,对乙醇浸膏进行萃取时,依次分步加入石油醚、乙酸乙酯、正丁醇150~200 m L得到萃取液,控制旋蒸、烘干温度在50℃以下,即可得到有生物活性的乙酸乙酯提取物和正丁醇提取物。乙酸乙酯提取物和正丁醇提取物的提取率约为0.1%。
