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Purification,structural elucidation,and in vitro antitumor effects of novel polysaccharides from Bangia fuscopurpurea 被引量:6
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作者 Jingna Wu Xiaoting Chen +2 位作者 Kun Qiao Yongchang Su Zhiyu Liu 《Food Science and Human Wellness》 SCIE 2021年第1期63-71,共9页
A water-soluble polysaccharide,designated BFP-3,was isolated from Bangia fuscopurpurea by hot water extraction,anion-exchange,and size-exclusion chromatography and tested to determine its antitumor activity.The struct... A water-soluble polysaccharide,designated BFP-3,was isolated from Bangia fuscopurpurea by hot water extraction,anion-exchange,and size-exclusion chromatography and tested to determine its antitumor activity.The structural characteristics of BFP-3 were investigated by chemical and spectroscopic methods,including partial acid hydrolysis,methylation analysis,one-and two-dimensional nuclear magnetic resonance,and gas chromatography-mass spectrometry.The results showed that BFP-3 was mainly comprised of rhamnose,arabinose,mannose,glucose,and galactose.Moreover,the weight-average molecular weight of BFP-3 was estimated to be approximately 333 kDa.The backbone of BFP-3 was primarily composed of repeating 5-α-l-Araf-1→(4-α-d-Glcp-1)_(4)→4,6-β-d-Manp-1 units,and the side chains consisted of repeatingβ-d-Galp-1→(4-β-d-Galp-1)_(4)→4,6-β-d-Galp-1→3,4-α-l-Rhap,β-l-Arap-1→(3-β-d-Galp-1)_(3),andβ-l-Arap-1 units.Counting Kit-8 assays revealed that BFP-3 significantly inhibited the proliferation of A2780,COC1,SKOV3,HO-8910,and OVCAR3 ovarian cancer cells in vitro,indicating that BFP-3 could have potential applications in the treatment of ovarian cancer. 展开更多
关键词 Bangia fuscopurpurea POLYSACCHARIDE Structure ovarian cancer antitumor effect
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Study on the Antitumor Effects of Low-energy Laser Irradiation Combined with Cyclophosphamide 被引量:1
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作者 HUANGBao-xu WANGHong-bin +3 位作者 QUZhi-na LIUHuan-qi LIUXi-feng CHENGShao-hui 《Journal of Northeast Agricultural University(English Edition)》 CAS 2003年第1期43-48,共6页
Systematic experiments about the antitumor effects of low energy laser irradiation combined with the traditional antitumor medicine of cyclophosphamide were conducted using the experimental model of mouse S180 ascite... Systematic experiments about the antitumor effects of low energy laser irradiation combined with the traditional antitumor medicine of cyclophosphamide were conducted using the experimental model of mouse S180 ascites sarcoma.The three groups of tumor bearing mice were irradiated upon the inner corners with the dosages of 11 00,14 67 and 22 00 J·cm -2 LELI respectively,and injected with CYT intraperitoneally to observe the changes of the survival time,the ascites growth speed,and the kinetic changes of immune functions.The survival times of the three groups of CYT/LELI combination were obviously longer than those of the tumor and CYT control groups.Correspondingly,the amounts of ascites,tumor cells densities and total tumor cells in CYT/LELI groups decreased significantly,while the death ratio of the tumor cells increased.Comparatively,the group of 22 00 J·cm -2 LELI combined with CYT showed the most ideal antitumor effects,and the life prolongation ratio was up to 53 20%. 展开更多
关键词 Low energy laser irradiation (LELI) cyclophosphamide(CYT) antitumor effects mouse S180 ascites sarcoma
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ANTITUMOR EFFECTS OF WICK OF SUN-FLOWER STEM IN MICE
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作者 李梅 苏树芸 +1 位作者 刘裕 章静波 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1992年第2期23-26,共4页
A well-known Chinese traditional drug, decoction of wick of sun-flower stem, has been used to treat cancer and certain other diseases.1,2 Animal experiments in mice showed that transplantation of Sarcoma 180 (S180) an... A well-known Chinese traditional drug, decoction of wick of sun-flower stem, has been used to treat cancer and certain other diseases.1,2 Animal experiments in mice showed that transplantation of Sarcoma 180 (S180) and of Uterus cervical cancer 14 (U14) were signlflcanly inhibited by this decoction. The Inhibition rates of tumor weight were 33 -81 % and in about 6 - 20% of treated mice, complete tumor regression has been demonstrated. So tar no acute toxic side-effect was noted. This is in contrast to most of the antitumor chemltherapeutic drugs known to produce different degrees of toxlcity or induce Immunosuppression as a side effect. For this reason, we have undertaken the present study. 展开更多
关键词 RATE antitumor effectS OF WICK OF SUN-FLOWER STEM IN MICE
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ANTITUMOR EFFECT OF ACTINIDIA CHINENSIS POLYSACCHARIDE ON MURINE TUMOR
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作者 林佩芳 张菊明 +2 位作者 徐杭民 何一中 郑宜和 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1989年第4期49-52,共4页
A new polysaccharide compound (ACPS-R) has recently been isolated from the root of Actinidia Chinensis Planch. When given inttaperitoneally to the transplantable tumor bearing mice at dose of 75-125 mg/kg, the tumor i... A new polysaccharide compound (ACPS-R) has recently been isolated from the root of Actinidia Chinensis Planch. When given inttaperitoneally to the transplantable tumor bearing mice at dose of 75-125 mg/kg, the tumor inhibition rate was more than 88.8% in Ehrilich ascitic cancer (EAC) or ascitic from of hepatoma (HepA) and more than 49.6% in solid hepatoma (HepS). The treatment effect of ACPS-R on EAC at dose of 80-100 mg kg and 125 mg/kg were comparable to that of cyclophosphamide at dose of 15 mg/kg and 22.5 mg/kg, respectively. ACPS-R could also prolong the life of EAC- or P388-bearing mice, and increase the percentage of EAC-free mice. In addition, when ACPS-R was used in combination with 5-Fu, the antitumor effect was enhanced as compared with 5-Fu alone. A marked increase in cAMP levels end cAMP cGMP ratio of spleen of EAC-bearing mice were observed after treatment of ACPS-R. The increase of both para meters nearly reached the normal levels of healthy mice. The increases of cAMP, cAMP cGMP and tumor remission had statistical significance. It showed an intermediate inhibitory effect of ACPS-R on DNA synthesis by incorporating 3H-TdR into EAC cells.The results indicated that ACPS-R acts as a new antitumor polysaccharide, and the treatment effect of Actinidia root in folk medicine is probably related to ACPS-R. 展开更多
关键词 EAC antitumor effect OF ACTINIDIA CHINENSIS POLYSACCHARIDE ON MURINE TUMOR
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Systematic literature review of the antitumor effect of octreotide in neuroendocrine tumors
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作者 Stephanie M Barrows Beilei Cai +3 位作者 Catherine Copley-Merriman Kelly R Wright Colleen V Castro Raoudha Soufi-Mahjoubi 《World Journal of Meta-Analysis》 2018年第2期9-20,共12页
AIM To provide a comprehensive examination of the existing evidence of the antitumor effect of long-acting octreotide in neuroendocrine tumors(NETs).METHODS A systematic literature review of clinical trials and observ... AIM To provide a comprehensive examination of the existing evidence of the antitumor effect of long-acting octreotide in neuroendocrine tumors(NETs).METHODS A systematic literature review of clinical trials and observational studies was conducted in PubM ed, EMBASE, and Cochrane through January 18, 2017. Conference abstracts for 2015 and 2016 from 5 scientific meetings were also searched.RESULTS Of 41 articles/abstracts identified, 13 unique studies compared octreotide with active or no treatment. Two of the 13 studies were clinical trials; the remaining were observational studies. The phase 3 Placebo-Controlled, Double-Blind, Prospective, Randomized Study of the Effect of Octreotide long-acting repeatable(LAR) in the Control of Tumor Growth in Patients with MetastaticNeuroendocrine Midgut Tumors clinical trial showed that long-acting octreotide significantly prolonged time to tumor progression compared with placebo in patients with functionally active and inactive metastatic midgut NETs; no statistically significant difference in overall survival(OS) was observed, possibly due to the crossover of placebo patients to octreotide. Retrospective observational studies found that long-acting octreotide use was associated with significantly longer OS than no octreotide use for patients with distant metastases although not for those with local/regional disease. CONCLUSION The clinical trial and observational studies with informative evidence support long-acting octreotide's antitumor effect on time to tumor progression and OS. This review showed the rarity of existing studies assessing octreotide's antitumor effect and recommends that future research is warranted. 展开更多
关键词 NEUROENDOCRINE TUMORS antitumor effect OCTREOTIDE Overall SURVIVAL Progression-free SURVIVAL
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Antitumor and synergistic effect of Chinese medicine “Bushen huayu jiedu recipe” and chemotherapy on transplanted animal hepatocarcinoma 被引量:7
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作者 Yong Cao Qing-Hua Xia +1 位作者 Hua Meng An-Pu Zhong 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第33期5218-5220,共3页
AIM: To investigate the antitumor and synergistic effect of Chinese medicine “Bushen huayu jiedu recipe” (recipe for invigorating the kidney, removing blood stasis and toxic substances) and chemotherapy on mice h... AIM: To investigate the antitumor and synergistic effect of Chinese medicine “Bushen huayu jiedu recipe” (recipe for invigorating the kidney, removing blood stasis and toxic substances) and chemotherapy on mice hepatocarcinoma. METHODS: Bushen huayu jiedu recipe (BSHYJDR) consisting of Chinese Cassia Bark, Psoralea, Zedoary, Rhubarb, etc. is equal to 1.5 g/mL liquid of originated herbs after being decoded, filtered, and concentrated. Kunming mice, weighing 18-22 g, were injected with 0.2 mL ascitic hepatocarcinoma H22 containing 1 × 10^7 cells/mL into armpit of the right forelimb of mice. After 24 h, the mice were weighed and randomly divided into tumor-bearing model control group, cisplatin (DDP) group, BSHYJDR high dosage group, low dosage BSHYJDR group, DDP combined with high and low dosage BSHYJDR group, 10 mice in each group. DDP group received injection intraperitoneally (ip) at the dosage of 1 mg/kg (equal to 1/10 LD50), once a day for 4 d. High and low dosage BSHYJDR groups received intragastric BSHYJDR at the dosages of 26.6 and 13.3 g/kg (20 and 10 times each of clinical adult dosage) respectively, while tumor-bearing model group received the equal volume of distilled water once a day for 10 d. On the 11^th d, the mice were weighed and killed, then the tumor was dissected and weighed, the repression rate (RR) was calculated according to the mean weight of tumor (MWT). RESULTS: Compared to the model group (MWT: 1.30±0.73), DDP group (MWT: 0.41±0.09, RR: 68.46%) had a significant difference in the inhibition of hepatocarcinoma H22 (P〈0.01). High dosage BSHYJDR group (MWT: 0.69±0.29, RR: 46.92%) also had a significant difference in inhibition (P〈0.05), while no difference was found in low dosage BSHYJDR group (MVVT: 0.85±0.34, RR: 34.62%) (P〉0.05). When DDP was combined with high dosage BSHYJDR (MWT: 0.29±0.17, RR: 77.69%) and low dosage BSHYJDR (MWT: 0.38±0.21, RR: 70.77%) respectively, we could see improvement of the inhibition effect of DDP on transplanted hepatocarcinoma H22. DDP combined with high dosage BSHYJDR had a significant difference (P〈0.001) compared to DDP, while DDP combined with low dosage BSHYJDR only had a little improvement that is not remarkable. CONCLUSION: Chinese medicine BSHYJDR in combination with chemotherapy can inhibit transplanted hepatocarcinorna in mice. 展开更多
关键词 Bushen huayu jiedu recipe Mice hepatocarcinoma H22 antitumor effects Synergy
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Enhancement of Antitumor Effect of Tegafur/Uracil(UFT) plus Leucovorin by Combined Treatment with Protein-Bound Polysaccharide,PSK,in Mouse Models 被引量:3
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作者 Ryoji Katoh Mitsuru Ooshiro 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2007年第4期295-299,共5页
We evaluated the antitumor effect of combined therapy with tegafur/uracil (UFT) plus leucovorin (LV) (UFT/LV) and protein-bound polysaccharide, PSK, in three mouse models of transplantable tumors. UFT/LV showed ... We evaluated the antitumor effect of combined therapy with tegafur/uracil (UFT) plus leucovorin (LV) (UFT/LV) and protein-bound polysaccharide, PSK, in three mouse models of transplantable tumors. UFT/LV showed antitumor effect against Meth A sarcoma, and the antitumor effect was enhanced when PSK given concomitantly. UFT/LV showed antitumor effect to Lewis lung carcinoma and PSK alone also showed antitumor effect at high dose, but a combination of UFT/LV and PSK resulted in no enhanced antitumor effect. Colon 26 carcinoma was weakly responsive to UFT/LV, and no enhancement of antitumor effect was found even PSK was used in combination. In conclusion, while the effect of PSK varies depending on tumor, combined use of UFT/LV and PSK may be expected to augment the antitumor effect. 展开更多
关键词 UFF LEUCOVORIN PSK Meth A Lewis lung carcinoma colon 26 antitumor effect
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Antitumor Effects of Ethanol Extract from Ventilago leiocarpa Benth on Sarcoma 180 Tumor-Bearing Mice and Possible Immune Mechanism 被引量:4
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作者 CHENG Dao-Hai LIU Ying WANG Li 《Chinese Journal of Integrative Medicine》 SCIE CAS CSCD 2021年第12期905-911,共7页
Objective To explore the antitumor effects of ethanol extract from Ventilago leiocarpa Benth(EEVLB)on sarcoma 180(S180)tumor-bearing mice and the potential mechanism.Methods Sixty mice were randomly assigned to 6 grou... Objective To explore the antitumor effects of ethanol extract from Ventilago leiocarpa Benth(EEVLB)on sarcoma 180(S180)tumor-bearing mice and the potential mechanism.Methods Sixty mice were randomly assigned to 6 groups according to a random number table:normal group,model group,5-fluorouracil(5-FU)group(0.02 g·kg^(−1)),and high-,medium-,low-dose EEVLB groups(100,84,and 56 g of raw material·kg^(−1)body weight,respectively),with 10 mice each group.All treatments were given once daily for 10 consecutive days.Effects of EEVLB on inhibiting tumor growth and immune function in mice were evaluated among all groups after the treatments by detecting tumor inhibition rate,organ index,serum levels of interleukin(IL)-2,-6,-10,CD3^(+)CD4^(+)T lymphocytes,CD4^(+)/CD8^(+)ratio,caspase-3 and Bcl-2.Results EEVLB with different concentrations achieved inhibition of tumor growth in vivo,wherein the high-dose group showed the most significant reduction in tumor weight and increased apoptosis of tumor cells(P<0.05).In addition,both net weight gain and spleen index of mice showed uptrend in EEVLB treatment groups(P<0.05).Besides,serum levels of IL-2 and IL-6,percentages of CD3^(+)CD4^(+)T lymphocytes and ratio of CD4^(+)/CD8^(+)in peripheral blood were elevated in high-and medium-dose EEVLB groups compared with the model group(P<0.05).Also,upregulation of caspase-3 and downregulation of Bcl-2 were observed at protein levels in the high-dose EEVLB group(P<0.01).Conclusions EEVLB exhibits promising antitumor activity in vivo.This effect might be due to activation of apoptotic signaling pathway,increase of cytokine levels and enhancement of immune function in tumor-bearing mice. 展开更多
关键词 Ventilago leiocarpa Benth Chinese folk medicine Yao medicine antitumor effect immune function CASPASE-3 BCL-2
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In vitro antitumor effect of cucurbitacin E on human lung cancer cell line and its molecular mechanism 被引量:4
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作者 JING Si-Yuan WU Zi-Dan +2 位作者 ZHANG Tie-Hua ZHANG Jie WEI Zheng-Yi 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2020年第7期483-490,共8页
Cucurbitacin E(CuE) is previously reported to exhibit antitumor effect by several means.In this study, CuE acted as a tyrosine kinase inhibitor interfering with the epidermal growth factor receptor/mitogen-activated p... Cucurbitacin E(CuE) is previously reported to exhibit antitumor effect by several means.In this study, CuE acted as a tyrosine kinase inhibitor interfering with the epidermal growth factor receptor/mitogen-activated protein kinase(EGFR/MAPK) signaling pathway and subsequently induced apoptosis and cell cycle arrest in non-small-cell lung cancer(NSCLC) cell line A549.The apoptosis regulators, cleaved Caspases-3 and Caspases-9, were observed to be increased with the treatment of CuE.The activated transcription factor STAT3 and the apoptosis inhibitor protein survivin were also observed to be reduced.The cell cycle regulators, CyclinA2, cylinB1, CyclinD1 and CyclinE, were also investigated and the results suggested that the cell cycle was arrested at G1/G0 phase.Treatment of CuE also altered the existence status of most of the participants in the EGFR/MAPK signaling.Phosphorylation of EGFR enhanced significantly, leading to the alteration of members downstream, either total amount or phosphorylation level, notably,MEK1/2 and ERK1/2.Moreover, the results of molecular simulation brought an insight on the interaction mechanism between CuE and EGFR.In summary, CuE exhibited anti-proliferative effect against A549 cells by targeting the EGFR/MAPK signaling pathway. 展开更多
关键词 Cucurbitacin E antitumor effect Lung cancer cell APOPTOSIS Cell cycle arrest
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PLASMID VACCINE ENCODING HN GENE FROM NEWCASTLE DISEASE VIRUS HAS MARKED ANTITUMORAL EFFECT IN VITRO
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作者 薛立娟 金宁一 +2 位作者 龚伟 王宏伟 李萍 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2003年第3期161-166,共6页
Objective: To explore the antitumor effects of hemaagglutinin-neuraminase gene (HN gene) from Newcastle disease virus. Methods: Plasmid vaccine of pIRHN was constructed and transfected into HeLa cells. The expression... Objective: To explore the antitumor effects of hemaagglutinin-neuraminase gene (HN gene) from Newcastle disease virus. Methods: Plasmid vaccine of pIRHN was constructed and transfected into HeLa cells. The expression of HN was analyzed by Western blot analysis, and the mode of cell death was detected by fluorescence microscope, gel electrophoresis and TUNEL assay and the expression of p53 and bcl-2 was also analyzed in transfected Hela cells. The effect of pIRHN on sialic acid contents in the Hela cell was examined. Results: pIRHN nucleic acid vaccines could be expressed in eukaryotic cell. pIRHN could induce apoptosis after HeLa cells were transfected. The effect of antitumor responses of pIRHN was correlated with the contents of sialic acid in tumor cells, and there was no prominent evidence for the relatedness of the antitumor effect with the expression of p53 and bcl-2. Conclusion: pIRHN may become a new antitumor biological agent. 展开更多
关键词 Newcastle disease virus Hemaagglutinin- neuraminase gene Western blot antitumor effect Nucleic acid vaccines Apoptosis
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EFFECT OF ANTITUMOR DRUGS ON THE ACTIVITY OF DNA TOPOISOMERASE I
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作者 钱毅 曾桂超 张迺蘅 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1990年第4期40-44,共5页
The activity of DNA topoisomerase Ⅱ prepared from either normal or tumor tissues were compared. It was found that the unknotting activity of the enzyme in malignant tumor cells was higher than that in normal cells. W... The activity of DNA topoisomerase Ⅱ prepared from either normal or tumor tissues were compared. It was found that the unknotting activity of the enzyme in malignant tumor cells was higher than that in normal cells. We selected some antitumor drugs including Chinese traditional medicine, and observed their effects on the unknotting activity of topoisomerase Ⅱ. The results showed that inhibition of the unknotting activity of the enzyme required very low concentrations of drugs, but much higher concentrations were required for other tested. Some antitumor drugs had no effect on the enzyme were also proved. It is interesting that carrageenan, an antiviral drug, strongly blocked the unknotting activity although its antitumor activity has not been reported. 展开更多
关键词 DNA effect OF antitumor DRUGS ON THE ACTIVITY OF DNA TOPOISOMERASE I
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THE ANTITUMOR ACTIVITY OF LYMPHOTOXIN AND THE EFFECT OF INTERLEUKIN-2 ON ITS FUNCTION
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作者 沈泽荔 何俊森 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1990年第4期45-49,共5页
The effect of lymphotoxin (LT)-containing supernatant produced by lectin-stimulated human lymphocytes on tumor cells and the relation between interleukin-2 (IL-2) and LT were studied in this article. Results showed th... The effect of lymphotoxin (LT)-containing supernatant produced by lectin-stimulated human lymphocytes on tumor cells and the relation between interleukin-2 (IL-2) and LT were studied in this article. Results showed that LT-containing superna-tants had cytotoxicities on many different kinds of tumor cells from human and mice, that actinomycin D increased the LT activities on target cells and that IL-2 had the ability to increase the cytotoxicity of human PBMC on tumor cells, after being treated with LT, the target cells were more easy to kill by PBMC as well. 展开更多
关键词 THE antitumor ACTIVITY OF LYMPHOTOXIN AND THE effect OF INTERLEUKIN-2 ON ITS FUNCTION MTT
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通消阳和颗粒联合环磷酰胺对4T1乳腺癌荷瘤小鼠移植瘤的影响
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作者 陈晶 林晓峰 +1 位作者 孙跃峰 张月 《中医药学报》 CAS 2024年第8期31-39,共9页
目的:观察通消阳和颗粒联合环磷酰胺对4T1乳腺癌荷瘤小鼠的作用。方法:选取BALB/c雌性小鼠建立4T1乳腺癌荷瘤模型,随机分成模型组、通消阳和颗粒组(颗粒组)、通消阳和颗粒^(+)环磷酰胺组(联合组)及环磷酰胺组,每组8只,另选取8只作为空... 目的:观察通消阳和颗粒联合环磷酰胺对4T1乳腺癌荷瘤小鼠的作用。方法:选取BALB/c雌性小鼠建立4T1乳腺癌荷瘤模型,随机分成模型组、通消阳和颗粒组(颗粒组)、通消阳和颗粒^(+)环磷酰胺组(联合组)及环磷酰胺组,每组8只,另选取8只作为空白组。监测各组荷瘤小鼠肿瘤生长情况,绘制乳腺肿瘤时间-生长曲线;计算各组小鼠肿瘤抑制率;检测外周血白细胞计数及中性粒细胞、淋巴细胞和单核细胞的比例;测定肝、肾功能的生化指标;检测脾脏免疫细胞数量及CD4^(+)T和CD8^(+)T细胞比例;培养各组小鼠肺、脑、肝及外周血细胞,用于检测肿瘤细胞远处器官转移情况。结果:与模型组相比,联合组小鼠移植瘤增长速度最缓慢(P<0.01),抑瘤率最高(P<0.01),白细胞计数显著降低(P<0.01);与环磷酰胺组相比,联合组小鼠淋巴细胞比例明显升高(P<0.01),中性粒细胞及单核细胞比例降低,差异有统计学意义(P<0.01),肝肾功能指标正常,无早期损害,T、B淋巴细胞升高、MDSCs细胞降低及CD4^(+)T和CD8^(+)T细胞比例升高,差异有统计学意义(P<0.05),乳腺癌细胞无远处器官转移情况。结论:通消阳和颗粒联合环磷酰胺可显著抑制乳腺癌荷瘤小鼠移植瘤生长;通消阳和颗粒可以减少环磷酰胺所致的小鼠肝肾功能损害、免疫功能减低等毒性反应,联合环磷酰胺可以明显提高乳腺癌小鼠的免疫功能并抑制肿瘤细胞的远处转移。 展开更多
关键词 通消阳和颗粒 环磷酰胺 乳腺癌 抑瘤作用 远处转移
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小檗碱药物的研究进展 被引量:1
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作者 张海霞 梅梅 《广东化工》 CAS 2024年第1期75-77,85,共4页
代谢性疾病发病率的增加严重影响了全世界的人类健康和生命安全。从天然化合物中寻找治疗代谢性疾病的有效药物具有重要意义。小檗碱一种重要的季铵类生物碱,多存在于许多传统药用植物中,近年来,动物和临床研究都证明,小檗碱及其代谢产... 代谢性疾病发病率的增加严重影响了全世界的人类健康和生命安全。从天然化合物中寻找治疗代谢性疾病的有效药物具有重要意义。小檗碱一种重要的季铵类生物碱,多存在于许多传统药用植物中,近年来,动物和临床研究都证明,小檗碱及其代谢产物对代谢性疾病具有良好的治疗效果。本文综述了小檗碱合成及提取工艺,及其在糖尿病、高尿酸血症等代谢性疾病中的药理活性,为进一步开发和利用小檗碱提供更加坚实的理论依据。 展开更多
关键词 小檗碱 合成及提取 抗菌作用 抗肿瘤作用 降血糖作用
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载阿霉素纳米粒温敏凝胶复合体系的体内缓释性能和抗肿瘤作用及瘤内滞留性能评价
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作者 杜华康 陆苑 +4 位作者 金阳 陈玉颖 王永林 李勇军 刘文 《贵州医科大学学报》 CAS 2024年第3期361-367,374,共8页
目的探讨载阿霉素(DOX)纳米粒温敏凝胶(DOX-NPs-Gel)复合体系的体内缓释性能、抗肿瘤作用及瘤内滞留性能。方法18只SD雄性大鼠随机均分为DOX组、DOX-碘油组(4 g/L DOX溶液1 mL与碘油1 mL混匀现配,5 mL/kg)及DOX-NPs-Gel组(5 mg/kg DOX+2... 目的探讨载阿霉素(DOX)纳米粒温敏凝胶(DOX-NPs-Gel)复合体系的体内缓释性能、抗肿瘤作用及瘤内滞留性能。方法18只SD雄性大鼠随机均分为DOX组、DOX-碘油组(4 g/L DOX溶液1 mL与碘油1 mL混匀现配,5 mL/kg)及DOX-NPs-Gel组(5 mg/kg DOX+2 g/L DOX),腹腔注射,分别于给药后不同时间点经眼底静脉丛穿刺取血,采用超高效液相色谱-串联质谱(UPLC-MS/MS)技术检测血浆中DOX的浓度,利用WinNonLin 8.1软件拟合主要药代参数[半衰期(t 1/2)、峰浓度(C max)、曲线下面积(AUC)、清除率(CL)、平均驻留时间(MRT)];培养、收集小鼠肝癌细胞株H22细胞制成悬液,单次接种于90只雄性ICR小鼠右腋皮下构建荷瘤模型,分2部分进行实验,第一部分取荷瘤小鼠36只均分为生理盐水组(注射用生理盐水,5 mL/kg)、空白NPs-Gel组(100 mg空白NPs冻干粉分散于1 mL空白Gel,5 mL/kg)、碘油组(碘油,5 mL/kg)、DOX组(2 g/L DOX,5 mL/kg)、DOX-碘油组(4 g/L DOX溶液1 mL与碘油1 mL混匀现配,5 mL/kg)及DOX-NPs-Gel组(2 g/L DOX,5 mL/kg),瘤内注射、给药1次,观测10 d,给药后每2天称体质量1次并计算各组小鼠的体质量变化百分比,称重后同时测量各组小鼠瘤体宽度、长度并计算肿瘤体积(V),观测结束时脱颈处死、剥离肿瘤组织,称取各组小鼠瘤体质量并计算抑瘤率,然后制作切片采用苏木精-伊红(HE)染色观察组织学特征;第二部分取荷瘤小鼠54只均分为DOX组、DOX-碘油组及DOX-NPs-Gel组,给药剂量和途径同前,给药后12、24、48、72、96及120 h时取各组3只小鼠脱颈处死,剥离肿瘤组织,采用UPLC-MS/MS检测瘤体组织中各时间点的DOX瘤内滞留率。结果体内缓释性能研究结果显示,相比于其余组,DOX-NPs-Gel组大鼠t 1/2延长、CL降低(P<0.05);抗肿瘤作用考察结果显示,与其它治疗组相比,DOX-NPs-Gel组小鼠肿瘤组织生长最慢,瘤重降低(P<0.05),抑瘤率最高(76.55%),HE染色显示肿瘤组织出现大范围凋亡和坏死情况;瘤内滞留性能考察结果显示,DOX-NPs-Gel组小鼠第5天时滞留率为(44.14±3.84)%,其余组第4天时滞留率已降至0%。结论与DOX和DOX-碘油相比,DOX-NPs-Gel在动物体内具有更强的缓释性能、抗肿瘤作用及瘤内滞留性能。 展开更多
关键词 纳米粒 温敏凝胶 药代动力学 抗肿瘤作用 缓释作用 肝动脉化疗栓塞术
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紫杉醇PLGA纳米粒的表征及体外抗肿瘤作用研究
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作者 王晓静 郭子硕 +4 位作者 张海桐 陈宛灵 李加玲 杜守颖 李鹏跃 《中国药房》 CAS 北大核心 2024年第22期2721-2725,共5页
目的表征紫杉醇纳米粒(PTX-PLGA-NPs),并评价其对Lewis肺癌细胞的体外抑制作用。方法对以乳化溶剂挥发法所制PTX-PLGA-NPs的粒径、多分散性指数(PDI)、Zeta电位、微观形态、包封率、载药量、紫外-可见光吸收特性、稳定性等进行表征;以小... 目的表征紫杉醇纳米粒(PTX-PLGA-NPs),并评价其对Lewis肺癌细胞的体外抑制作用。方法对以乳化溶剂挥发法所制PTX-PLGA-NPs的粒径、多分散性指数(PDI)、Zeta电位、微观形态、包封率、载药量、紫外-可见光吸收特性、稳定性等进行表征;以小鼠Lewis肺癌细胞为对象,以PTX对照品为参照,分别采用CCK-8法、Calcein-AM/PI双染法检测PTX-PLGA-NPs的细胞毒性和体外杀伤活性,分别采用AnnexinⅤ-FITC/PI染色法、PI染色法评估PTX-PLGA-NPs对细胞凋亡及周期的影响。结果PTXPLGA-NPs呈类球形,平均粒径为(172.03±0.95)nm,PDI为0.098±0.012,Zeta电位为(-1.76±0.02)mV;包封率和载药量分别为(52.32±0.66)%、(7.07±0.18)%,紫外-可见光吸收特征不受载体聚乳酸-羟基乙酸共聚物的影响;4℃下避光放置7 d时,其粒径无明显变化,平均PDI(放置1、2、4、7 d)均小于0.3。与PTX对照品组相比,PTX-PLGA-NPs组有更多细胞处于死亡状态,其存活率(当PTX质量浓度为11.2μg/mL时)显著降低,凋亡率和G2期细胞比例均显著升高(P<0.05)。结论所制PTX-PLGA-NPs粒径均一、分散均匀、性质稳定,对肺癌细胞的体外杀伤作用较PTX强。 展开更多
关键词 紫杉醇 聚乳酸-羟基乙酸共聚物 纳米粒 表征 LEWIS肺癌细胞 体外抗肿瘤作用
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Inhibitory effect of acetylshikonin on human gastric carcinoma cell line SGC-7901 in vitro and in vivo 被引量:10
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作者 Yun Zeng Gang Liu Li-Ming Zhou 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第15期1816-1820,共5页
AIM:To investigate the inhibitory effect of acetylshikonin on human gastric carcinoma cell line SGC-7901 and its mechanism. METHODS:MTT assay was used to assess the inhibitory effect of acetylshikonin on proliferation... AIM:To investigate the inhibitory effect of acetylshikonin on human gastric carcinoma cell line SGC-7901 and its mechanism. METHODS:MTT assay was used to assess the inhibitory effect of acetylshikonin on proliferation of SGC-7901 cells.Apopt osis-inducing effect was determined by flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling with Hoechst staining.Expression of mRNA and protein in Bcl-2 and Bax was analyzed by reverse transcription-polymerase chain reaction and Western blot.Antitumor effect of acetylshikonin on a mouse SGC-7901 model was also determined. RESULTS:Forty-eight hours after treatment with acetylshikonin,MTT assay showed that acetylshikonin inhibited the proliferation of SGC-7901 cells in a dose-dependent manner.The half maximal inhibitory concentration of acetylshikonin to SGC-7901 cells was 0.428±0.07 mg/L.Cell shrinkage,nuclear pyknosis and chromatin condensation,which are the characteristics of cell apoptosis,were observed in treated SGC-7901 cells and the percentage of apoptosis increased in a dose-dependent manner.Acetylshikonin downregulated the expression of Bcl-2 and up-regulated the expression of Bax in the treated SGC-7901 cells compared with the controls.The experiment in vivo showed that 0.5,1,and 2 mg/kg of acetylshikonin significantly inhibited the growth of tumor in the mouse SGC-7901 model,with an inhibitory rate of 25.00%-55.76%. CONCLUSION:Acetylshikonin inhibits the growth of SGC-7901 cells in vitro and in vivo by inducing cell apoptosis. 展开更多
关键词 Acetylshikonin antitumor effect SGC-7901cells APOPTOSIS
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HCA587/MAGE-C2蛋白联合CFA和CpG佐剂的免疫应答及抗肿瘤效应
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作者 焦薇 谭媛芳 +3 位作者 陈惠媛 范秋颖 尹艳慧 陈娟娟 《中国免疫学杂志》 CAS CSCD 北大核心 2024年第3期572-576,共5页
目的:探究HCA587/MAGE-C2蛋白在不同免疫策略下诱导抗原特异性免疫应答的能力及在小鼠模型中的抗肿瘤作用。方法:HCA587蛋白与弗氏完全佐剂(CFA)/弗氏不完全佐剂(IFA)、不同剂量CpG ODN 1826(CpG)联合免疫C57BL/6J小鼠,比较不同方案诱... 目的:探究HCA587/MAGE-C2蛋白在不同免疫策略下诱导抗原特异性免疫应答的能力及在小鼠模型中的抗肿瘤作用。方法:HCA587蛋白与弗氏完全佐剂(CFA)/弗氏不完全佐剂(IFA)、不同剂量CpG ODN 1826(CpG)联合免疫C57BL/6J小鼠,比较不同方案诱导的细胞免疫和体液免疫水平。酶联免疫斑点试验(ELISpot)检测脾细胞产生IFN-γ的频率;ELISA检测抗HCA587特异性抗体;流式细胞术分析胞内细胞因子染色(ICCS)。将B16-HCA587肿瘤细胞皮下接种于C57BL/6J小鼠右侧胁腹部建立荷瘤小鼠模型,采用免疫比较方案中细胞和体液免疫应答最强的策略进行治疗;游标卡尺测量肿瘤体积;Log-rank检验评估生存曲线。结果:HCA587蛋白联合CFA和50μg CpG免疫方案诱导出能分泌抗原特异性IFN-γ的脾细胞频数最多,诱导抗HCA587的IgG抗体滴度最高,诱导出的IFN-γ^(+)CD4^(+)T细胞比例最高(P<0.05)。肿瘤治疗模型中,HCA587蛋白联合CFA和50μg CpG可显著抑制肿瘤生长(P=0.026),但Log-rank检验显示其对生存期无明显影响(P>0.05)。结论:佐剂CFA和50μg CpG联合组成的HCA587蛋白疫苗在小鼠模型中能诱导强大的细胞免疫应答和体液免疫应答,并具有一定抗肿瘤作用,为肿瘤抗原蛋白疫苗临床前研究提供了新的实验数据。 展开更多
关键词 HCA587蛋白 佐剂 IFN-Γ CD4^(+)T细胞 抗肿瘤作用
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Preparation, characterization, pharmacokinetics and anticancer effects of PEGylated β-elemene liposomes 被引量:6
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作者 Bingtao Zhai Qibiao Wu +15 位作者 Wengang Wang Mingming Zhang Xuemeng Han Qiujie Li Peng Chen Xiaying Chen Xingxing Huang Guohua Li Qin Zhang Ruonan Zhang Yu Xiang Shuiping Liu Ting Duan Jianshu Lou Tian Xie Xinbing Sui 《Cancer Biology & Medicine》 SCIE CAS CSCD 2020年第1期60-75,共16页
Objective:This study aimed to develop a new polyethylene glycol(PEG)ylatedβ-elemene liposome(PEG-Lipo-β-E)and evaluate its characterization,pharmacokinetics,antitumor effects and safety in vitro and in vivo.Methods:... Objective:This study aimed to develop a new polyethylene glycol(PEG)ylatedβ-elemene liposome(PEG-Lipo-β-E)and evaluate its characterization,pharmacokinetics,antitumor effects and safety in vitro and in vivo.Methods:The liposomes were prepared by ethanol injection and high-pressure micro-jet homogenization.Characterization of the liposomes was conducted,and drug content,entrapment efficiency(EE),in vitro release and stability were studied by ultra-fast liquid chromatography(UFLC)and a liquid surface method.Blood was drawn from rats to establish the pharmacokinetic parameters.The anticancer effect was evaluated in a KU-19-19 bladder cancer xenograft model.Histological analyses were performed to evaluate safety.Results:The PEG-Lipo-β-E showed good stability and was characterized as 83.31±0.181 nm in size,0.279±0.004 in polydispersity index(PDI),-21.4±1.06 mV in zeta potential,6.65±0.02 in pH,5.024±0.107 mg/mL inβ-elemene(β-E)content,and 95.53±1.712%in average EE.The Fourier transform infrared spectroscopy(FTIR)and differential scanning calorimetry(DSC)indicated the formation of PEG-Lipo-β-E.Compared to elemene injection,PEG-Lipo-β-E demonstrated a 1.75-fold decrease in clearance,a 1.62-fold increase in half-life,and a 1.76-fold increase in area under the concentration-time curves(AUCs)from 0 hour to 1.5 hours(P<0.05).PEG-Lipo-β-E also showed an enhanced anticancer effect in vivo.Histological analyses showed that there was no evidence of toxicity to the heart,kidney,liver,lung or spleen.Conclusions:The present study demonstrates PEG-Lipo-β-E as a new formulation with ease of preparation,high EE,good stability,improved bioavailability and antitumor effects. 展开更多
关键词 Β-ELEMENE PEGylated liposome PHARMACOKINETICS antitumor effect bladder cancer
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The Inhibitory Effect of Oridonin on the Growth of Fifteen Human Cancer Cell Lines 被引量:32
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作者 Junhui Chen Shaobin Wang +4 位作者 Dongyang Chen Guisheng Chang Qingfeng Xin Shoujun Yuan Zhongying Shen 《Chinese Journal of Clinical Oncology》 CSCD 2007年第1期16-20,共5页
OBJECTIVE To study the inhibitory effect of oridonin on the growth of cancer cells. METHODS Fifteen human cancer cell lines were subjected to various concentrations of oridonin in culture medium. The inhibitory rate o... OBJECTIVE To study the inhibitory effect of oridonin on the growth of cancer cells. METHODS Fifteen human cancer cell lines were subjected to various concentrations of oridonin in culture medium. The inhibitory rate of cell growth was measured by the MTT assay, and compared with a negative control and 5-Fu-positive control. RESULTS The 50% inhibiting concentration (IC50) and maximal inhibition (Imax) of oridonin shown by studying the growth of the cancer cell lines were as follows: leukemias (HL60 cells: 3.9 μg/ml and 73.8%, K562 cells: 4.3 μg/ml and 76.2%); esophageal cancers(SHEEC cells: 15.4 μg/ml and 99.2%, Eca109 cells: 15.1 μg/ml and 84.6%, TEl cells: 4.0 μg/ml and 70.2%); gastric cancers (BGC823 cells: 7.6 μg/ml and 98.7%, SGC7901 cells: 12.3 μg/ml and 85.7%); colon cancers (HT29 cells: 13.6 μg/ml and 97.2%, HCT cells: 14.5 μg/ml and 96.5%); liver cancers (Bel7402 cells: 15.2 μg/ml and 89.2%, HepG2 cells: 7.1 μg/ml and 88.3%); pancreatic cancer (PC3 cells: 11.3 μg/ml and 68.4%); lung cancer (A549 cells: 18.6 μg/ml and 98.0% ); breast cancer (MCF7 cells: 18.4 μg/ml and 84.7%); uterine cervix cancer (Hela cells: 13.7 μg/ml and 98.5%). CONCLUSION Oridonin had a relatively wide anti-tumor spectrum, and a relatively strong inhibitory effect on the growth of the 15 human cancer cells. Inhibitory effects were concentration dependent. 展开更多
关键词 ORIDONIN antitumor effect in vitro study.
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