Objective:To investigate the effects of electroacupuncture(EA)on the behaviors of rat with anxiety disorder,and the expressions of hippocampal neurotransmitters including 5-hydroxytryptamine(5-HT),norepinephrine(NE)an...Objective:To investigate the effects of electroacupuncture(EA)on the behaviors of rat with anxiety disorder,and the expressions of hippocampal neurotransmitters including 5-hydroxytryptamine(5-HT),norepinephrine(NE)and dopamine(DA),and the expressions of hippocampal B-cell lymphoma-2(Bcl-2)and Bcl-2 associated X(Bax).Methods:Forty-six male Wistar rats were randomly divided into a control group(n=10);a model group(n=12),an EA group(n=12),and a drug group(n=12).Except the control group,the other three groups were established into rat models of anxiety disorder using uncertain empty bottle stimulation.Rats in the EA group and the drug group received corresponding interventions for 15 consecutive days[EA group was given EA at Baihui(GV 20)and Sanyinjiao(SP 6);the drug group was given aqueous solution of alprazolam via intragastric administration].After intervention,all four groups received open-field test(OFT)and elevated plus-maze(EPM)for behavioral evaluations.The expressions of 5-HT,NE and DA in hippocampus were determined by fluorescence spectroscopy(FS)while the expressions of Bcl-2 and Bax proteins in hippocampus were determined by Western blot(WB).Results:The OFT horizontal scores in the control group,EA group and drug group were significantly higher than that in the model group(all P<0.05),and the difference between the EA group and the drug group was statistically insignificant(P>0.05);the OFT vertical scores in the model group,EA group and drug group were significantly lower than the score in the control group(all P<0.05).The EPM percent of open-arm entries(OE%)in the control group,EA group and drug group was higher than that in the model group(P<0.05),and the differences among these three groups were statistically insignificant(P>0.05);though the percent of open-arm total time(OT%)in the EA group was lower than that in the control group(P<0.05);the difference was statistically insignificant when compared with the drug group(P>0.05),and it was significantly higher than that in the model group(P<0.05).The expression of 5-HT in the EA group was higher than that in the control group(P<0.05);the expression of 5-HT in the EA group was significantly lower than that in the model group(P<0.05);the difference between the EA group and the drug group was statistically insignificantly(P>0.05).The expression of NE in the model group was significantly higher than that in the other three groups(P<0.0S),and there was no significant difference among these three groups(P>0.05).The expression of DA in the EA group was significantly higher than that in the control group and the drug group(both P<0.05),while the difference between the EA group and the model group was statistically insignificant(P>0.05).The expression of Bax in the model group was significantly higher than that in the other three groups(all P<0.05),whereas the expression of Bcl-2 in the model group was significantly lower than that in the other three groups(all P<0.05),and the differences in both Bax and Bcl-2 among the other three groups were statistically insignificant(all P>0.05).Bax/Bcl-2 in the EA group was significantly higher than that in the control group(P<0.05)and lower than that in the model group(P<0.05),and the difference was statistically insignificant when compared with the drug group(P>0.05).Conclusion:EA shows promising effects in attenuating rats'anxiety disorder,which may be achieved by the down-regulation of the expressions of 5-HT and NE in the hippocampus and/or inhibition of hippocampal neuronal apoptosis.The efficacy is comparable to that of intervention with alprazolam.展开更多
文摘Objective:To investigate the effects of electroacupuncture(EA)on the behaviors of rat with anxiety disorder,and the expressions of hippocampal neurotransmitters including 5-hydroxytryptamine(5-HT),norepinephrine(NE)and dopamine(DA),and the expressions of hippocampal B-cell lymphoma-2(Bcl-2)and Bcl-2 associated X(Bax).Methods:Forty-six male Wistar rats were randomly divided into a control group(n=10);a model group(n=12),an EA group(n=12),and a drug group(n=12).Except the control group,the other three groups were established into rat models of anxiety disorder using uncertain empty bottle stimulation.Rats in the EA group and the drug group received corresponding interventions for 15 consecutive days[EA group was given EA at Baihui(GV 20)and Sanyinjiao(SP 6);the drug group was given aqueous solution of alprazolam via intragastric administration].After intervention,all four groups received open-field test(OFT)and elevated plus-maze(EPM)for behavioral evaluations.The expressions of 5-HT,NE and DA in hippocampus were determined by fluorescence spectroscopy(FS)while the expressions of Bcl-2 and Bax proteins in hippocampus were determined by Western blot(WB).Results:The OFT horizontal scores in the control group,EA group and drug group were significantly higher than that in the model group(all P<0.05),and the difference between the EA group and the drug group was statistically insignificant(P>0.05);the OFT vertical scores in the model group,EA group and drug group were significantly lower than the score in the control group(all P<0.05).The EPM percent of open-arm entries(OE%)in the control group,EA group and drug group was higher than that in the model group(P<0.05),and the differences among these three groups were statistically insignificant(P>0.05);though the percent of open-arm total time(OT%)in the EA group was lower than that in the control group(P<0.05);the difference was statistically insignificant when compared with the drug group(P>0.05),and it was significantly higher than that in the model group(P<0.05).The expression of 5-HT in the EA group was higher than that in the control group(P<0.05);the expression of 5-HT in the EA group was significantly lower than that in the model group(P<0.05);the difference between the EA group and the drug group was statistically insignificantly(P>0.05).The expression of NE in the model group was significantly higher than that in the other three groups(P<0.0S),and there was no significant difference among these three groups(P>0.05).The expression of DA in the EA group was significantly higher than that in the control group and the drug group(both P<0.05),while the difference between the EA group and the model group was statistically insignificant(P>0.05).The expression of Bax in the model group was significantly higher than that in the other three groups(all P<0.05),whereas the expression of Bcl-2 in the model group was significantly lower than that in the other three groups(all P<0.05),and the differences in both Bax and Bcl-2 among the other three groups were statistically insignificant(all P>0.05).Bax/Bcl-2 in the EA group was significantly higher than that in the control group(P<0.05)and lower than that in the model group(P<0.05),and the difference was statistically insignificant when compared with the drug group(P>0.05).Conclusion:EA shows promising effects in attenuating rats'anxiety disorder,which may be achieved by the down-regulation of the expressions of 5-HT and NE in the hippocampus and/or inhibition of hippocampal neuronal apoptosis.The efficacy is comparable to that of intervention with alprazolam.
