旨在利用全基因组关联分析探寻敖汉细毛羊羊毛性状新的分子标记和候选基因。本研究采集1~2周岁的健康敖汉细毛羊耳组织与羊毛作为试验素材,其中,母羊248只,公羊81只,总计329只。羊毛进行性状测定(包括纤维直径、自然长度、伸直长度、伸...旨在利用全基因组关联分析探寻敖汉细毛羊羊毛性状新的分子标记和候选基因。本研究采集1~2周岁的健康敖汉细毛羊耳组织与羊毛作为试验素材,其中,母羊248只,公羊81只,总计329只。羊毛进行性状测定(包括纤维直径、自然长度、伸直长度、伸直率),并对表型数据进行描述性统计和相关性分析。利用绵羊40K液相SNP芯片对全部个体进行基因分型。使用Plink 1.07软件对芯片数据进行质控,使用GCTA软件和PopLDdecay软件对质控数据进行群体结构分析。利用GMEMA混合线性模型对4种羊毛性状进行了全基因组关联分析(genomewide association study,GWAS),利用在线软件对候选基因进行GO和KEGG富集分析。质控后得到329只个体的30079个SNPs位点用于后续分析。通过GWAS分析筛选出4个在全基因组上显著相关的SNPs位点可能影响羊毛经济性状,分别位于1号、6号及8号染色体上。筛选出9个在染色体水平上显著相关的SNPs位点可能对羊毛性状具有潜在意义,分别位于3、5、8、11、18、21、22、25号染色体,寻找到39个可能影响羊毛性状的候选基因。本研究结果为后续探究敖汉细毛羊羊毛性状的遗传机制及分子育种标记开发提供重要参考。展开更多
[Objective] To get major genes for wool traits regulation from immune genes. [Methods] Microarray technology was used to detect differentially expressed immune genes between body side skin （more wool growing） and gr...[Objective] To get major genes for wool traits regulation from immune genes. [Methods] Microarray technology was used to detect differentially expressed immune genes between body side skin （more wool growing） and groin skin （no wool growing） of Aohan fine wool sheep. [Results] 46 immune genes （fold change 〉2.0） were identified and classified, and then 6 of which were selected for QPCR confir- mation. The degree of consistency of the QPCR and microarray results was 66.67%, [Conclusion] Immune privilege may participate in wool growth regulation.展开更多
Background： Increasing the polyunsaturated fatty acid （PUFA） content and decreasing the saturated fatty acid （SFA） content of mutton can help to improve its nutritional value for consumers. Several laboratories h...Background： Increasing the polyunsaturated fatty acid （PUFA） content and decreasing the saturated fatty acid （SFA） content of mutton can help to improve its nutritional value for consumers. Several laboratories have evaluated the effects of vitamin E on the fatty acid （FA） composition of muscle in sheep. However, little information is available on wool sheep, even though wool sheep breeds are an important source of mutton, especially in northern China where sheep are extensively farmed. The present study was designed to address the effects of vitamin E on muscle FA composition in male Aohan fine-wool sheep. Methods： Forty-two male Aohan fine-wool lambs （5 mo old） with similar initial body weight were randomly divided into seven groups and fed diets supplemented with 0 （control group）, 20, 100, 200, 1,000, 2,000, or 2,400 IU/sheep/d vitamin E for 12 mo. Three lambs from each group were slaughtered to measure vitamin E and FA content in the Iongissimus lumborum （LL） and gluteus medius （GM） muscles. Results： Vitamin E concentrations in the LL and GM increased significantly after 12 mo of vitamin E supplementation （P 〈 0.05）. However, this increase did not occur in a dose-dependent manner because the muscle vitamin E concentration was highest in the 200 IU/sheep/d group. Dietary vitamin E supplementation also caused a significant reduction in SFA content and an increase in monounsaturated FA （MUFA） content in the LL and GM （P 〈 0.05）. All six doses of vitamin E significantly increased cis9 tronsl -conjugated linoleic acid （cgtl -CLA） content in the LL compared with the control group （P 〈 0.05）. Conclusions： Dietary supplementation with vitamin E increased muscle vitamin E content and improved the nutritional value of mutton by decreasing SFA content and increasing MUFA and c9tl 1-CLA contents in Aohan fine-wool sheep. These effects were greatest in sheep fed a diet containing 200 IU/sheep/d vitamin E.展开更多
The evolvement of a vulnerable ecological region is a dynamic process, which is affected by various factors. During the evolvement process, human activities have a decisive effect. The purpose of studying vulnerable e...The evolvement of a vulnerable ecological region is a dynamic process, which is affected by various factors. During the evolvement process, human activities have a decisive effect. The purpose of studying vulnerable ecological region is to control human economic activities and to develop a negative feedback modulation mechanism.This paper established a model of vulnerable ecological region's evolvement by considering four synthetic variables.These synthetic variables are ecological carrying capacity, ecological resilience, economic development intensity, and economic development velocity. Finally, Ongniud Banner and Aohan Banner in North China were taken as study cases to simulate the evolvement processes of vulnerable ecological regions under different conditions of economic development. The results show that human activities have an important influence on the evolvement trend of vulnerable ecological region.展开更多
文摘旨在利用全基因组关联分析探寻敖汉细毛羊羊毛性状新的分子标记和候选基因。本研究采集1~2周岁的健康敖汉细毛羊耳组织与羊毛作为试验素材,其中,母羊248只,公羊81只,总计329只。羊毛进行性状测定(包括纤维直径、自然长度、伸直长度、伸直率),并对表型数据进行描述性统计和相关性分析。利用绵羊40K液相SNP芯片对全部个体进行基因分型。使用Plink 1.07软件对芯片数据进行质控,使用GCTA软件和PopLDdecay软件对质控数据进行群体结构分析。利用GMEMA混合线性模型对4种羊毛性状进行了全基因组关联分析(genomewide association study,GWAS),利用在线软件对候选基因进行GO和KEGG富集分析。质控后得到329只个体的30079个SNPs位点用于后续分析。通过GWAS分析筛选出4个在全基因组上显著相关的SNPs位点可能影响羊毛经济性状,分别位于1号、6号及8号染色体上。筛选出9个在染色体水平上显著相关的SNPs位点可能对羊毛性状具有潜在意义,分别位于3、5、8、11、18、21、22、25号染色体,寻找到39个可能影响羊毛性状的候选基因。本研究结果为后续探究敖汉细毛羊羊毛性状的遗传机制及分子育种标记开发提供重要参考。
基金Supported by Project of National Hair Sheep Industry Technology System(CARS-40)~~
文摘[Objective] To get major genes for wool traits regulation from immune genes. [Methods] Microarray technology was used to detect differentially expressed immune genes between body side skin （more wool growing） and groin skin （no wool growing） of Aohan fine wool sheep. [Results] 46 immune genes （fold change 〉2.0） were identified and classified, and then 6 of which were selected for QPCR confir- mation. The degree of consistency of the QPCR and microarray results was 66.67%, [Conclusion] Immune privilege may participate in wool growth regulation.
基金financially supported by projects 200903060CARS-39 from China Agricultural Ministry
文摘Background： Increasing the polyunsaturated fatty acid （PUFA） content and decreasing the saturated fatty acid （SFA） content of mutton can help to improve its nutritional value for consumers. Several laboratories have evaluated the effects of vitamin E on the fatty acid （FA） composition of muscle in sheep. However, little information is available on wool sheep, even though wool sheep breeds are an important source of mutton, especially in northern China where sheep are extensively farmed. The present study was designed to address the effects of vitamin E on muscle FA composition in male Aohan fine-wool sheep. Methods： Forty-two male Aohan fine-wool lambs （5 mo old） with similar initial body weight were randomly divided into seven groups and fed diets supplemented with 0 （control group）, 20, 100, 200, 1,000, 2,000, or 2,400 IU/sheep/d vitamin E for 12 mo. Three lambs from each group were slaughtered to measure vitamin E and FA content in the Iongissimus lumborum （LL） and gluteus medius （GM） muscles. Results： Vitamin E concentrations in the LL and GM increased significantly after 12 mo of vitamin E supplementation （P 〈 0.05）. However, this increase did not occur in a dose-dependent manner because the muscle vitamin E concentration was highest in the 200 IU/sheep/d group. Dietary vitamin E supplementation also caused a significant reduction in SFA content and an increase in monounsaturated FA （MUFA） content in the LL and GM （P 〈 0.05）. All six doses of vitamin E significantly increased cis9 tronsl -conjugated linoleic acid （cgtl -CLA） content in the LL compared with the control group （P 〈 0.05）. Conclusions： Dietary supplementation with vitamin E increased muscle vitamin E content and improved the nutritional value of mutton by decreasing SFA content and increasing MUFA and c9tl 1-CLA contents in Aohan fine-wool sheep. These effects were greatest in sheep fed a diet containing 200 IU/sheep/d vitamin E.
基金Under the auspices of the National Natural Science Foundation of China (No. 40301052)the 10th Five-year National Key Technology R&D Program of Ministry of Science and Technology (No. 2001BA606A-01)
文摘The evolvement of a vulnerable ecological region is a dynamic process, which is affected by various factors. During the evolvement process, human activities have a decisive effect. The purpose of studying vulnerable ecological region is to control human economic activities and to develop a negative feedback modulation mechanism.This paper established a model of vulnerable ecological region's evolvement by considering four synthetic variables.These synthetic variables are ecological carrying capacity, ecological resilience, economic development intensity, and economic development velocity. Finally, Ongniud Banner and Aohan Banner in North China were taken as study cases to simulate the evolvement processes of vulnerable ecological regions under different conditions of economic development. The results show that human activities have an important influence on the evolvement trend of vulnerable ecological region.
