A reserved-phase HPLC method was developed for the determination of barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller, and whether there was a close relationship between the contents o...A reserved-phase HPLC method was developed for the determination of barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller, and whether there was a close relationship between the contents of barbaloin and their environments in which they were growing was decided. A Hypersil ODS column (4.6 mm×200 mm, 5 μm)was used with a mobile phase of methanol-water (40:60, containing 0.1% acetic acid), the flow rate being 1.0 mL·min -1, detection wavelength at 359 nm, and the column temperature being 30℃. The linear range of barbaloin was between 0.0726 and 0.726 μg with a correlation coefficient of 0.9998 and the regression equation being Y=1.9202×10 6X-1801.9. Barbaloin was stable in methanol in 48 h and the instrument precision was 1.2% while the method precision was 4.9%. The contents of barbaloin of 12 samples ranged from 6.160 to 319.1 μg·g -1. The method developed was fast and simple with good reproducibility. There was high correlation between the contents of barbaloin and their growing environments.展开更多
Objective: This paper mainly determined the action modes of extract of Aloe vera L. against Tetranychus cinnabarinus Boisduval. Methods: The different action modes, contact action, repellent, fumigant, and oviposition...Objective: This paper mainly determined the action modes of extract of Aloe vera L. against Tetranychus cinnabarinus Boisduval. Methods: The different action modes, contact action, repellent, fumigant, and oviposition inhibition property of the acetone extract of Aloe vera L. leaf against the carmine spider mite Tetranychus cinnabaribus (Boisduval) (Acarina: Tetranychidae) were investigated at 26°C ± 1°C, 75% - 80% relative humidity, and 14:10 light: day cycle in the laboratory. Results: Based on the established toxicity regression line of the Aloe vera L. acetone extract against female adult mites, the median lethal concentrations (LC50) were 0.836 and 0.167 mg/mL for 48 and 72 h, respectively. With processing time increased, the contact acaricidal activity increased and the repellent activity gradually decreased. The main modes of action of the extract against female adult mites were contact and repellent, and preferable effects were observed on adult mites. These results indicate that A. vera L. extract contains acaricidal and repellent bioactive components that may be useful in future control of the phytophagous mites.展开更多
This study attempts to examine the effects of salinity on leaf, stem, and nut of Pistacia vera （Ohady and Kaleh Ghochi cultivars） in order to characterize their anatomical structures with electron and light microsco...This study attempts to examine the effects of salinity on leaf, stem, and nut of Pistacia vera （Ohady and Kaleh Ghochi cultivars） in order to characterize their anatomical structures with electron and light microscopy. A comparative study of the anatomy indicates a difference between the density of simple and glandular trichomes in leaf surface, deposit of wax, the thickness of leaf, the length of epidermal cells, palisade parenchyma cells, crystals in mesophyll, and the manner of compatibility to salinity. The results obtained in this research demonstrate that compatibility of Ohady cultivar is higher than Kaleh Ghochi and the degree of mechanical wounding depends on their development stages. In addition to results of previous studies which considered crop load as the main cause of fruit deformity. The present study indicates that, in spite of some reduction in total production of a tree, salinity decreases fruit deformity and to a high extent prevents the production of deformed fruit. Indeed, a remarkable reduction in production of deformed fruit will be yielded by selecting pistachio varieties which are resistant to salinity. Moreover, salinity increases the resistance of nuts to mechanical wounding. The results of this study suggest that in EC = 7.6, 12.6 deformity of nut is not related to crop load.展开更多
[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia...[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia vera ＇Kerman＇,tissue culture condition and medium and transplant matrix were researched for micropropagation of pistachio,[Result]Mediums of 1/2DKW and 1/2DKW＋3.00 mg/L of 6-BA ＋0.05 mg/L of NAA were suitable medium for seed germination and axillary bud induction of pistachio; Proliferation coefficient was 3.6 on the medium of 1/2DKW ＋4.00 mg/L of 6-BA ＋ 0.05 mg/L of IBA; Rooting rate was up to 75% on the medium of 1/2DKW ＋ 5.00 mg/L of IBA ＋ 1.00 mg/L of NAA; The suitable transplantation matrix was 3/4 sand ＋ 1/4 vermiculite,and survival rate of plant with root was above 79%. [Conclusion]The pistachio （Kerman） tissue culture technology system was established.展开更多
文摘A reserved-phase HPLC method was developed for the determination of barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller, and whether there was a close relationship between the contents of barbaloin and their environments in which they were growing was decided. A Hypersil ODS column (4.6 mm×200 mm, 5 μm)was used with a mobile phase of methanol-water (40:60, containing 0.1% acetic acid), the flow rate being 1.0 mL·min -1, detection wavelength at 359 nm, and the column temperature being 30℃. The linear range of barbaloin was between 0.0726 and 0.726 μg with a correlation coefficient of 0.9998 and the regression equation being Y=1.9202×10 6X-1801.9. Barbaloin was stable in methanol in 48 h and the instrument precision was 1.2% while the method precision was 4.9%. The contents of barbaloin of 12 samples ranged from 6.160 to 319.1 μg·g -1. The method developed was fast and simple with good reproducibility. There was high correlation between the contents of barbaloin and their growing environments.
文摘Objective: This paper mainly determined the action modes of extract of Aloe vera L. against Tetranychus cinnabarinus Boisduval. Methods: The different action modes, contact action, repellent, fumigant, and oviposition inhibition property of the acetone extract of Aloe vera L. leaf against the carmine spider mite Tetranychus cinnabaribus (Boisduval) (Acarina: Tetranychidae) were investigated at 26°C ± 1°C, 75% - 80% relative humidity, and 14:10 light: day cycle in the laboratory. Results: Based on the established toxicity regression line of the Aloe vera L. acetone extract against female adult mites, the median lethal concentrations (LC50) were 0.836 and 0.167 mg/mL for 48 and 72 h, respectively. With processing time increased, the contact acaricidal activity increased and the repellent activity gradually decreased. The main modes of action of the extract against female adult mites were contact and repellent, and preferable effects were observed on adult mites. These results indicate that A. vera L. extract contains acaricidal and repellent bioactive components that may be useful in future control of the phytophagous mites.
文摘This study attempts to examine the effects of salinity on leaf, stem, and nut of Pistacia vera （Ohady and Kaleh Ghochi cultivars） in order to characterize their anatomical structures with electron and light microscopy. A comparative study of the anatomy indicates a difference between the density of simple and glandular trichomes in leaf surface, deposit of wax, the thickness of leaf, the length of epidermal cells, palisade parenchyma cells, crystals in mesophyll, and the manner of compatibility to salinity. The results obtained in this research demonstrate that compatibility of Ohady cultivar is higher than Kaleh Ghochi and the degree of mechanical wounding depends on their development stages. In addition to results of previous studies which considered crop load as the main cause of fruit deformity. The present study indicates that, in spite of some reduction in total production of a tree, salinity decreases fruit deformity and to a high extent prevents the production of deformed fruit. Indeed, a remarkable reduction in production of deformed fruit will be yielded by selecting pistachio varieties which are resistant to salinity. Moreover, salinity increases the resistance of nuts to mechanical wounding. The results of this study suggest that in EC = 7.6, 12.6 deformity of nut is not related to crop load.
基金Supported by Beijing Natural Science Foundation (6072005)Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality(PXM2009-014207-076874)~~
文摘[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia vera ＇Kerman＇,tissue culture condition and medium and transplant matrix were researched for micropropagation of pistachio,[Result]Mediums of 1/2DKW and 1/2DKW＋3.00 mg/L of 6-BA ＋0.05 mg/L of NAA were suitable medium for seed germination and axillary bud induction of pistachio; Proliferation coefficient was 3.6 on the medium of 1/2DKW ＋4.00 mg/L of 6-BA ＋ 0.05 mg/L of IBA; Rooting rate was up to 75% on the medium of 1/2DKW ＋ 5.00 mg/L of IBA ＋ 1.00 mg/L of NAA; The suitable transplantation matrix was 3/4 sand ＋ 1/4 vermiculite,and survival rate of plant with root was above 79%. [Conclusion]The pistachio （Kerman） tissue culture technology system was established.
